pycom07g24690

Actin depolymerisation factor/cofilin -like domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0028852
Physical Location & Seq
Forward (+)
25307674 .. 25308611
938 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 621 bp
ATGACTTTTCATTTCTTTGCAGCCACAGAAGCTTTAGAGAATTCTCAGCCTTTGTTTTGCCCCTTAAAATTCTCTTCCCCTCCGCAATTTTCTGGTCTCAGCCTTTCCCCCCAAAAACAAGGATTTCTCCTTCTGCTGCTAAACTTTCCATCTCTCTTTCTTTTCCGGCCTTCTTCTTCATTTCGCACTACACTCCGTCTCTTTTCCGAGGACAAAATGGCGTCCGGCATGGCCGTGAATGATGAATGCAAGCTCAAGTTCGTGGAGCTAAAAGCGAAGAGAAACTATCGATTCATCGTATTCAAGATTGACAAACAAGAAGTGGTGGTAGAGAAAGTTGGCAGCCCAGATGAAACATACGAGGATTTCGCGGCGTCGCTGCCTGCTGATGAGTGCCGCTATGCTGTCTTTGATTTTGATTTCACCACTGCTGAGAACTGCCAGAAAAGCAAGATTTTCTTCATTGCATGGTCTCCTGAGACATCAAAGGTGAGAATGAAGATGGTGTATGCAAGCTCTAAAGATCGATTCAAGAGGGAACTCGACGGCATTCAGGTCGAGTTGCAGGCAACCGATCCGAGCGAAATGAGCCTCGACATTATTAAAGGCCGAGCTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF zf-CCHC zf-CCHC_4

Protein Analysis

207

Amino Acids

23.56

Weight (kDa)

6.62

Isoelectric Point (pI)

49.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 76 - 204 1.5e-41 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028852)

Species Orthologous Gene IDs
malus_domestica MD07G1269800.v1.1
pyrus_communis pycom07g24690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 371
AciI CCGC 3 cut(s) 83, 371, 397
AclWI GGATC 1 cut(s) 569
AcoI YGGCCR 1 cut(s) 231
AcsI RAATTY 2 cut(s) 40, 68
AcyI GRCGYC 2 cut(s) 221, 374
AgsI TTSAA 2 cut(s) 304, 532
AluBI AGCT 5 cut(s) 32, 253, 268, 516, 614
AluI AGCT 5 cut(s) 32, 253, 268, 516, 614
Alw21I GWGCWC 1 cut(s) 616
Alw26I GTCTC 4 cut(s) 101, 203, 473, 477
AlwI GGATC 1 cut(s) 569
AoxI GGCC 3 cut(s) 167, 231, 607
ApeKI GCWGC 4 cut(s) 20, 136, 342, 379
ApoI RAATTY 2 cut(s) 40, 68
AsuHPI GGTGA 2 cut(s) 415, 502
BanII GRGCYC 1 cut(s) 616
Bbv12I GWGCWC 1 cut(s) 616
BbvI GCAGC 4 cut(s) 32, 123, 354, 366
BccI CCATC 2 cut(s) 157, 496
BceAI ACGGC 2 cut(s) 218, 562
BcgI CGANNNNNNTGC 2 cut(s) 538, 572
BcoDI GTCTC 4 cut(s) 101, 203, 473, 477
BisI GCNGC 6 cut(s) 21, 137, 343, 372, 380, 397
BlsI GCNGC 6 cut(s) 22, 138, 344, 373, 381, 398
BpuEI CTTGAG 1 cut(s) 239
Bsa29I ATCGAT 2 cut(s) 289, 526
BsaHI GRCGYC 2 cut(s) 221, 374
BsaI GGTCTC 2 cut(s) 101, 477
BsaJI CCNNGG 1 cut(s) 207
Bse3DI GCAATG 1 cut(s) 462
BseCI ATCGAT 2 cut(s) 289, 526
BseDI CCNNGG 1 cut(s) 207
BseMI GCAATG 1 cut(s) 462
BseMII CTCAG 4 cut(s) 59, 112, 423, 468
BseXI GCAGC 4 cut(s) 32, 123, 354, 366
Bsh1236I CGCG 1 cut(s) 371
BshFI GGCC 3 cut(s) 169, 233, 609
BshVI ATCGAT 2 cut(s) 289, 526
BsiHKAI GWGCWC 1 cut(s) 616
BsiSI CCGG 2 cut(s) 166, 225
BsmAI GTCTC 4 cut(s) 101, 203, 473, 477
BsmBI CGTCTC 1 cut(s) 203
BsmI GAATGC 2 cut(s) 251, 549
BsnI GGCC 3 cut(s) 169, 233, 609
Bso31I GGTCTC 2 cut(s) 101, 477
Bsp1286I GDGCHC 1 cut(s) 616
Bsp143I GATC 2 cut(s) 523, 574
BspACI CCGC 3 cut(s) 83, 371, 397
BspANI GGCC 3 cut(s) 169, 233, 609
BspCNI CTCAG 4 cut(s) 58, 111, 424, 469
BspDI ATCGAT 2 cut(s) 289, 526
BspFNI CGCG 1 cut(s) 371
BspPI GGATC 1 cut(s) 569
BspTNI GGTCTC 2 cut(s) 101, 477
BsrDI GCAATG 1 cut(s) 462
BssECI CCNNGG 1 cut(s) 207
BssMI GATC 2 cut(s) 523, 574
BssNI GRCGYC 2 cut(s) 221, 374
Bst6I CTCTTC 2 cut(s) 79, 272
BstACI GRCGYC 2 cut(s) 221, 374
BstC8I GCNNGC 4 cut(s) 251, 384, 514, 567
BstDEI CTNAG 4 cut(s) 45, 98, 432, 477
BstFNI CGCG 1 cut(s) 371
BstKTI GATC 2 cut(s) 526, 577
BstMAI GTCTC 4 cut(s) 101, 203, 473, 477
BstMBI GATC 2 cut(s) 523, 574
BstMWI GCNNNNNNNGC 3 cut(s) 29, 447, 588
BstUI CGCG 1 cut(s) 371
BstV1I GCAGC 4 cut(s) 32, 123, 354, 366
Bsu15I ATCGAT 2 cut(s) 289, 526
BsuRI GGCC 3 cut(s) 169, 233, 609
BsuTUI ATCGAT 2 cut(s) 289, 526
BtsI GCAGTG 1 cut(s) 426
BtsIMutI CAGTG 1 cut(s) 426
Cac8I GCNNGC 4 cut(s) 251, 384, 514, 567
ClaI ATCGAT 2 cut(s) 289, 526
CseI GACGC 2 cut(s) 210, 363
CviAII CATG 2 cut(s) 229, 468
DdeI CTNAG 4 cut(s) 45, 98, 432, 477
DpnI GATC 2 cut(s) 525, 576
DpnII GATC 2 cut(s) 523, 574
EaeI YGGCCR 1 cut(s) 231
Eam1104I CTCTTC 2 cut(s) 79, 272
EarI CTCTTC 2 cut(s) 79, 272
Ecl136II GAGCTC 1 cut(s) 614
Eco24I GRGCYC 1 cut(s) 616
Eco31I GGTCTC 2 cut(s) 101, 477
Eco53kI GAGCTC 1 cut(s) 614
EcoICRI GAGCTC 1 cut(s) 614
EcoRI GAATTC 1 cut(s) 40
EcoT38I GRGCYC 1 cut(s) 616
Esp3I CGTCTC 1 cut(s) 203
FaeI CATG 2 cut(s) 232, 471
FaiI YATR 5 cut(s) 230, 358, 402, 469, 510
FalI AAGNNNNNCTT 2 cut(s) 443, 475
FatI CATG 2 cut(s) 228, 467
Fnu4HI GCNGC 6 cut(s) 21, 137, 343, 372, 380, 397
FriOI GRGCYC 1 cut(s) 616
Fsp4HI GCNGC 6 cut(s) 21, 137, 343, 372, 380, 397
GluI GCNGC 6 cut(s) 21, 137, 343, 372, 380, 397
HaeIII GGCC 3 cut(s) 169, 233, 609
HapII CCGG 2 cut(s) 166, 225
HgaI GACGC 2 cut(s) 210, 363
Hin1I GRCGYC 2 cut(s) 221, 374
Hin1II CATG 2 cut(s) 232, 471
HindIII AAGCTT 1 cut(s) 30
HinfI GANTC 2 cut(s) 291, 528
HpaII CCGG 2 cut(s) 166, 225
HphI GGTGA 2 cut(s) 415, 502
Hpy188I TCNGA 2 cut(s) 208, 579
Hpy188III TCNNGA 3 cut(s) 304, 476, 532
Hpy99I CGWCG 2 cut(s) 379, 548
HpyAV CCTTC 2 cut(s) 140, 180
HpyCH4V TGCA 5 cut(s) 20, 249, 467, 512, 565
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 447, 588
HpyF3I CTNAG 4 cut(s) 45, 98, 432, 477
Hsp92I GRCGYC 2 cut(s) 221, 374
Hsp92II CATG 2 cut(s) 232, 471
Kzo9I GATC 2 cut(s) 523, 574
LmnI GCTCC 1 cut(s) 265
LpnPI CCDG 9 cut(s) 78, 179, 238, 360, 396, 455, 489, 539, 551
Lsp1109I GCAGC 4 cut(s) 32, 123, 354, 366
MalI GATC 2 cut(s) 525, 576
MboI GATC 2 cut(s) 523, 574
MboII GAAGA 6 cut(s) 66, 165, 168, 289, 451, 511
MhlI GDGCHC 1 cut(s) 616
MluCI AATT 3 cut(s) 40, 68, 86
MnlI CCTC 5 cut(s) 90, 202, 355, 528, 602
MseI TTAA 3 cut(s) 65, 603, 619
MslI CAYNNNNRTG 1 cut(s) 233
MspI CCGG 2 cut(s) 166, 225
Mva1269I GAATGC 2 cut(s) 251, 549
MvnI CGCG 1 cut(s) 371
MwoI GCNNNNNNNGC 3 cut(s) 29, 447, 588
NdeII GATC 2 cut(s) 523, 574
NlaIII CATG 2 cut(s) 232, 471
PctI GAATGC 2 cut(s) 251, 549
PfeI GAWTC 2 cut(s) 291, 528
PkrI GCNGC 6 cut(s) 22, 138, 344, 373, 381, 398
Psp124BI GAGCTC 1 cut(s) 616
RseI CAYNNNNRTG 1 cut(s) 233
SacI GAGCTC 1 cut(s) 616
SaqAI TTAA 3 cut(s) 65, 603, 619
SatI GCNGC 6 cut(s) 21, 137, 343, 372, 380, 397
Sau3AI GATC 2 cut(s) 523, 574
SduI GDGCHC 1 cut(s) 616
SetI ASST 7 cut(s) 34, 255, 270, 492, 518, 558, 616
SmiMI CAYNNNNRTG 1 cut(s) 233
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 3 cut(s) 40, 68, 86
SsiI CCGC 3 cut(s) 83, 371, 397
SstI GAGCTC 1 cut(s) 616
TaqI TCGA 5 cut(s) 289, 526, 543, 558, 594
TasI AATT 3 cut(s) 40, 68, 86
TauI GCSGC 2 cut(s) 374, 399
TfiI GAWTC 2 cut(s) 291, 528
Tru1I TTAA 3 cut(s) 65, 603, 619
Tru9I TTAA 3 cut(s) 65, 603, 619
TscAI CASTG 1 cut(s) 433
TseI GCWGC 4 cut(s) 20, 136, 342, 379
TspDTI ATGAA 6 cut(s) 168, 258, 283, 366, 451, 512
TspGWI ACGGA 1 cut(s) 185
TspRI CASTG 1 cut(s) 433
XapI RAATTY 2 cut(s) 40, 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.