pycom07g26520

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0029335
Physical Location & Seq
Forward (+)
26514947 .. 26515830
884 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 600 bp
ATGCACATTTGTCACCTCAAGTGCATCAAAATATCTGAGCAAACGGGTCATGGATCCGCGGGTCGGGGCGGGGCGTGGTGGGGACAAATTTCAGAGAAAACGGGCCCTCGGTCCGAACCTTTTCCACCGTTCATTAAAGGGAGATTAAATTTCCACCGTTTATTTCTAACACATAGCCACATAGCTTATGTCGAATTCTCCAGACTCCAGAGTCCTCCCAGATCCCCGACTCTTCTCGACCTCGAATTCTCCCAACTTCGTCCATTCATTAACTGGCCGGCGTTCTTCTTAGTTTCAGCAACCATTTCCAGCCTCCAGTCGTCTGGGGTTAGTCGGTCTCCAGAGTCATTTCCCATTTCATCCATGCCTGGCAATGACGATGTCGGCTTCTCTGAGAAAGAGAAGAACCTCGAGCTCTTCCTCAAAGTCGGATTGGACGAGTGCACGGCCAAAAACATCGTCGCCAACAACAAAGTCACTGCCAACCTCACCATTGTCATTCACGAGGTTGCGGTTACTGATGGATGTAGTCGAACAGTTGGTAATCTCCTCTACAAGGATTTTCTCGCAGCGGCTAAGGAAGGAGCTTGGATGGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

200

Amino Acids

21.93

Weight (kDa)

8.38

Isoelectric Point (pI)

62.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_synt_1c_R1 PF04558 135 - 186 1.4e-10 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029335)

Species Orthologous Gene IDs
pyrus_communis pycom07g26520
rosa_multiflora Rmu_co8387699.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 210
AccII CGCG 1 cut(s) 59
AciI CCGC 5 cut(s) 57, 59, 69, 512, 572
AclWI GGATC 3 cut(s) 48, 61, 216
AcoI YGGCCR 2 cut(s) 275, 447
AcsI RAATTY 4 cut(s) 87, 148, 194, 245
AfiI CCNNNNNNNGG 2 cut(s) 63, 556
AjnI CCWGG 1 cut(s) 367
AluBI AGCT 3 cut(s) 185, 415, 587
AluI AGCT 3 cut(s) 185, 415, 587
Alw21I GWGCWC 2 cut(s) 417, 446
Alw26I GTCTC 1 cut(s) 342
Alw44I GTGCAC 1 cut(s) 442
AlwI GGATC 3 cut(s) 48, 61, 216
Ama87I CYCGRG 1 cut(s) 410
AoxI GGCC 3 cut(s) 103, 275, 447
ApaI GGGCCC 1 cut(s) 107
ApaLI GTGCAC 1 cut(s) 442
ApeKI GCWGC 1 cut(s) 569
ApoI RAATTY 4 cut(s) 87, 148, 194, 245
ArsI GACNNNNNNTTYG 2 cut(s) 444, 476
Asp700I GAANNNNTTC 1 cut(s) 120
AspS9I GGNCC 3 cut(s) 103, 104, 111
AsuHPI GGTGA 2 cut(s) 5, 481
AvaI CYCGRG 1 cut(s) 410
AvaII GGWCC 1 cut(s) 111
BaeGI GKGCMC 2 cut(s) 107, 446
BamHI GGATCC 1 cut(s) 53
BanII GRGCYC 2 cut(s) 107, 417
BauI CACGAG 1 cut(s) 503
Bbv12I GWGCWC 2 cut(s) 417, 446
BbvI GCAGC 1 cut(s) 581
BccI CCATC 2 cut(s) 515, 586
BceAI ACGGC 1 cut(s) 462
BciT130I CCWGG 1 cut(s) 369
BcoDI GTCTC 1 cut(s) 342
BisI GCNGC 2 cut(s) 570, 573
BlsI GCNGC 2 cut(s) 571, 574
Bme1390I CCNGG 1 cut(s) 369
Bme18I GGWCC 1 cut(s) 111
BmeT110I CYCGRG 1 cut(s) 410
BmgT120I GGNCC 3 cut(s) 103, 104, 111
BmiI GGNNCC 2 cut(s) 55, 105
BmrFI CCNGG 1 cut(s) 369
BmsI GCATC 1 cut(s) 33
BpmI CTGGAG 4 cut(s) 184, 191, 299, 324
Bpu10I CCTNAGC 1 cut(s) 576
BsaI GGTCTC 1 cut(s) 342
BsaJI CCNNGG 2 cut(s) 57, 107
Bsc4I CCNNNNNNNGG 2 cut(s) 63, 556
Bse118I RCCGGY 1 cut(s) 277
Bse1I ACTGG 2 cut(s) 278, 316
Bse3DI GCAATG 1 cut(s) 379
BseBI CCWGG 1 cut(s) 369
BseDI CCNNGG 2 cut(s) 57, 107
BseGI GGATG 3 cut(s) 359, 530, 597
BseLI CCNNNNNNNGG 2 cut(s) 63, 556
BseMI GCAATG 1 cut(s) 379
BseMII CTCAG 2 cut(s) 27, 384
BseNI ACTGG 2 cut(s) 278, 316
BseRI GAGGAG 1 cut(s) 539
BseSI GKGCMC 2 cut(s) 107, 446
BseXI GCAGC 1 cut(s) 581
Bsh1236I CGCG 1 cut(s) 59
BshFI GGCC 3 cut(s) 105, 277, 449
BsiHKAI GWGCWC 2 cut(s) 417, 446
BsiHKCI CYCGRG 1 cut(s) 410
BsiSI CCGG 1 cut(s) 278
BslFI GGGAC 1 cut(s) 96
BslI CCNNNNNNNGG 2 cut(s) 63, 556
BsmAI GTCTC 1 cut(s) 342
BsmFI GGGAC 1 cut(s) 96
BsnI GGCC 3 cut(s) 105, 277, 449
Bso31I GGTCTC 1 cut(s) 342
BsoBI CYCGRG 1 cut(s) 410
Bsp120I GGGCCC 1 cut(s) 103
Bsp1286I GDGCHC 3 cut(s) 107, 417, 446
Bsp143I GATC 2 cut(s) 53, 221
BspACI CCGC 5 cut(s) 57, 59, 69, 512, 572
BspANI GGCC 3 cut(s) 105, 277, 449
BspCNI CTCAG 2 cut(s) 28, 385
BspFNI CGCG 1 cut(s) 59
BspLI GGNNCC 2 cut(s) 55, 105
BspPI GGATC 3 cut(s) 48, 61, 216
BspQI GCTCTTC 1 cut(s) 422
BspTNI GGTCTC 1 cut(s) 342
BsrDI GCAATG 1 cut(s) 379
BsrFI RCCGGY 1 cut(s) 277
BsrI ACTGG 2 cut(s) 278, 316
BssAI RCCGGY 1 cut(s) 277
BssECI CCNNGG 2 cut(s) 57, 107
BssMI GATC 2 cut(s) 53, 221
BssSI CACGAG 1 cut(s) 503
Bst2BI CACGAG 1 cut(s) 503
Bst2UI CCWGG 1 cut(s) 369
Bst4CI ACNGT 3 cut(s) 129, 158, 538
Bst6I CTCTTC 2 cut(s) 237, 422
BstC8I GCNNGC 1 cut(s) 279
BstDEI CTNAG 4 cut(s) 36, 289, 393, 576
BstDSI CCRYGG 1 cut(s) 57
BstENI CCTNNNNNAGG 1 cut(s) 554
BstF5I GGATG 3 cut(s) 359, 530, 597
BstFNI CGCG 1 cut(s) 59
BstKTI GATC 2 cut(s) 56, 224
BstMAI GTCTC 1 cut(s) 342
BstMBI GATC 2 cut(s) 53, 221
BstNI CCWGG 1 cut(s) 369
BstSCI CCNGG 1 cut(s) 367
BstSLI GKGCMC 2 cut(s) 107, 446
BstUI CGCG 1 cut(s) 59
BstV1I GCAGC 1 cut(s) 581
BstX2I RGATCY 2 cut(s) 53, 221
BstXI CCANNNNNNTGG 1 cut(s) 323
BstYI RGATCY 2 cut(s) 53, 221
BsuRI GGCC 3 cut(s) 105, 277, 449
BtgI CCRYGG 1 cut(s) 57
BtsCI GGATG 3 cut(s) 359, 530, 597
BtsI GCAGTG 1 cut(s) 477
BtsIMutI CAGTG 1 cut(s) 477
Cac8I GCNNGC 1 cut(s) 279
Cfr10I RCCGGY 1 cut(s) 277
Cfr13I GGNCC 3 cut(s) 103, 104, 111
Cfr42I CCGCGG 1 cut(s) 60
CpoI CGGWCCG 1 cut(s) 111
CspI CGGWCCG 1 cut(s) 111
CviAII CATG 2 cut(s) 50, 364
DdeI CTNAG 4 cut(s) 36, 289, 393, 576
DpnI GATC 2 cut(s) 55, 223
DpnII GATC 2 cut(s) 53, 221
DrdI GACNNNNNNGTC 1 cut(s) 210
DseDI GACNNNNNNGTC 1 cut(s) 210
EaeI YGGCCR 2 cut(s) 275, 447
Eam1104I CTCTTC 2 cut(s) 237, 422
EarI CTCTTC 2 cut(s) 237, 422
Ecl136II GAGCTC 1 cut(s) 415
Eco24I GRGCYC 2 cut(s) 107, 417
Eco31I GGTCTC 1 cut(s) 342
Eco47I GGWCC 1 cut(s) 111
Eco53kI GAGCTC 1 cut(s) 415
Eco88I CYCGRG 1 cut(s) 410
EcoICRI GAGCTC 1 cut(s) 415
EcoNI CCTNNNNNAGG 1 cut(s) 554
EcoO109I RGGNCCY 1 cut(s) 104
EcoRI GAATTC 2 cut(s) 194, 245
EcoRII CCWGG 1 cut(s) 367
EcoT38I GRGCYC 2 cut(s) 107, 417
FaeI CATG 2 cut(s) 53, 367
FaiI YATR 5 cut(s) 51, 174, 182, 189, 365
FaqI GGGAC 1 cut(s) 96
FatI CATG 2 cut(s) 49, 363
FauI CCCGC 2 cut(s) 52, 62
Fnu4HI GCNGC 2 cut(s) 570, 573
FokI GGATG 2 cut(s) 346, 537
FriOI GRGCYC 2 cut(s) 107, 417
Fsp4HI GCNGC 2 cut(s) 570, 573
GluI GCNGC 2 cut(s) 570, 573
GsuI CTGGAG 4 cut(s) 184, 191, 299, 324
HaeIII GGCC 3 cut(s) 105, 277, 449
HapII CCGG 1 cut(s) 278
Hin1II CATG 2 cut(s) 53, 367
HinfI GANTC 4 cut(s) 204, 211, 229, 344
HpaII CCGG 1 cut(s) 278
HphI GGTGA 2 cut(s) 5, 481
Hpy166II GTNNAC 1 cut(s) 444
Hpy188I TCNGA 5 cut(s) 37, 94, 115, 394, 431
Hpy188III TCNNGA 5 cut(s) 201, 208, 236, 341, 503
Hpy8I GTNNAC 1 cut(s) 444
Hpy99I CGWCG 1 cut(s) 464
HpyAV CCTTC 1 cut(s) 575
HpyCH4III ACNGT 3 cut(s) 129, 158, 538
HpyCH4V TGCA 3 cut(s) 4, 24, 444
HpyF3I CTNAG 4 cut(s) 36, 289, 393, 576
Hsp92II CATG 2 cut(s) 53, 367
KroI GCCGGC 1 cut(s) 277
KroNI GCCGGC 1 cut(s) 279
KspI CCGCGG 1 cut(s) 60
Kzo9I GATC 2 cut(s) 53, 221
LguI GCTCTTC 1 cut(s) 422
LmnI GCTCC 1 cut(s) 584
Lsp1109I GCAGC 1 cut(s) 581
LweI GCATC 1 cut(s) 33
MaeIII GTNAC 3 cut(s) 11, 475, 514
MalI GATC 2 cut(s) 55, 223
MboI GATC 2 cut(s) 53, 221
MboII GAAGA 4 cut(s) 224, 277, 409, 415
MflI RGATCY 2 cut(s) 53, 221
MhlI GDGCHC 3 cut(s) 107, 417, 446
MluCI AATT 4 cut(s) 87, 148, 194, 245
MlyI GAGTC 4 cut(s) 198, 220, 223, 353
MmeI TCCRAC 1 cut(s) 409
MroNI GCCGGC 1 cut(s) 277
MroXI GAANNNNTTC 1 cut(s) 120
MseI TTAA 3 cut(s) 135, 146, 270
MspA1I CMGCKG 2 cut(s) 59, 572
MspI CCGG 1 cut(s) 278
MspR9I CCNGG 1 cut(s) 369
MvaI CCWGG 1 cut(s) 369
MvnI CGCG 1 cut(s) 59
NaeI GCCGGC 1 cut(s) 279
NdeII GATC 2 cut(s) 53, 221
NgoMIV GCCGGC 1 cut(s) 277
NlaIII CATG 2 cut(s) 53, 367
NlaIV GGNNCC 2 cut(s) 55, 105
NmuCI GTSAC 2 cut(s) 11, 475
PaeR7I CTCGAG 1 cut(s) 410
PciSI GCTCTTC 1 cut(s) 422
PcsI WCGNNNNNNNCGW 1 cut(s) 435
PdiI GCCGGC 1 cut(s) 279
PdmI GAANNNNTTC 1 cut(s) 120
PflFI GACNNNGTC 1 cut(s) 380
PkrI GCNGC 2 cut(s) 571, 574
PleI GAGTC 4 cut(s) 198, 219, 223, 352
PpsI GAGTC 4 cut(s) 198, 219, 223, 352
Psp124BI GAGCTC 1 cut(s) 417
Psp6I CCWGG 1 cut(s) 367
PspGI CCWGG 1 cut(s) 367
PspN4I GGNNCC 2 cut(s) 55, 105
PspOMI GGGCCC 1 cut(s) 103
PspPI GGNCC 3 cut(s) 103, 104, 111
PspXI VCTCGAGB 1 cut(s) 410
PsuI RGATCY 2 cut(s) 53, 221
PsyI GACNNNGTC 1 cut(s) 380
Rsr2I CGGWCCG 1 cut(s) 111
RsrII CGGWCCG 1 cut(s) 111
SacI GAGCTC 1 cut(s) 417
SacII CCGCGG 1 cut(s) 60
SapI GCTCTTC 1 cut(s) 422
SaqAI TTAA 3 cut(s) 135, 146, 270
SatI GCNGC 2 cut(s) 570, 573
Sau3AI GATC 2 cut(s) 53, 221
Sau96I GGNCC 3 cut(s) 103, 104, 111
SchI GAGTC 4 cut(s) 198, 220, 223, 353
ScrFI CCNGG 1 cut(s) 369
SduI GDGCHC 3 cut(s) 107, 417, 446
SetI ASST 9 cut(s) 18, 121, 187, 243, 411, 417, 489, 510, 589
SfaNI GCATC 1 cut(s) 33
Sfr274I CTCGAG 1 cut(s) 410
Sfr303I CCGCGG 1 cut(s) 60
SgrBI CCGCGG 1 cut(s) 60
SinI GGWCC 1 cut(s) 111
SlaI CTCGAG 1 cut(s) 410
SmlI CTYRAG 2 cut(s) 17, 410
SmoI CTYRAG 2 cut(s) 17, 410
Sse9I AATT 4 cut(s) 87, 148, 194, 245
SsiI CCGC 5 cut(s) 57, 59, 69, 512, 572
SstI GAGCTC 1 cut(s) 417
StyD4I CCNGG 1 cut(s) 367
TaaI ACNGT 3 cut(s) 129, 158, 538
TaqI TCGA 5 cut(s) 192, 237, 243, 411, 532
TaqII GACCGA 2 cut(s) 99, 324
TasI AATT 4 cut(s) 87, 148, 194, 245
TauI GCSGC 1 cut(s) 575
Tru1I TTAA 3 cut(s) 135, 146, 270
Tru9I TTAA 3 cut(s) 135, 146, 270
TscAI CASTG 1 cut(s) 484
TseFI GTSAC 2 cut(s) 11, 475
TseI GCWGC 1 cut(s) 569
Tsp45I GTSAC 2 cut(s) 11, 475
TspDTI ATGAA 3 cut(s) 121, 256, 348
TspRI CASTG 1 cut(s) 484
Tth111I GACNNNGTC 1 cut(s) 380
VneI GTGCAC 1 cut(s) 442
VpaK11BI GGWCC 1 cut(s) 111
XagI CCTNNNNNAGG 1 cut(s) 554
XapI RAATTY 4 cut(s) 87, 148, 194, 245
XcmI CCANNNNNNNNNTGG 1 cut(s) 270
XhoI CTCGAG 1 cut(s) 410
XmnI GAANNNNTTC 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.