pycom08g05820

Belongs to the universal ribosomal protein uS3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0029340
Physical Location & Seq
Reverse (-)
4385864 .. 4386420
557 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 366 bp
ATGGAGAGTGGTGCAAAGGGATGTGAGGTTATTGTTAGTGGAAAGCTCAGGGCAGCACGTGCCAAGTCAATGAAGTTTAAGGATGGGTACATGATTTCCTCTGGTCAGCCCGTCAATGAGTACATTGACTCAGCCGTGAGGCACGTTCTTCTTAGACAGTTAATTGTGTCTTTGCTCTGGCAACTGCAACAGGGTGTGCTTGGTATTAAGGTGAAGATCATGCTTGACTGGGATCCCAAGGGCAAGCAGGGACCAATGACTCCATTGCCTGATTTGGTGACCATCCACACCCCCAAGGAAGAACAGTATCTTCCCGTTCCACCCGCAGCAATCAACATAGAGCCAGTTCCAGTTGCAGTAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
KH_2 Ribosomal_S3_C

Protein Analysis

122

Amino Acids

13.24

Weight (kDa)

9.1

Isoelectric Point (pI)

50.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S3_C PF00189 1 - 73 9.7e-15 Ribosomal protein S3, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029340)

Species Orthologous Gene IDs
pyrus_communis pycom08g05820
rosa_multiflora Rmu_sc0010056.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 324
AclWI GGATC 2 cut(s) 227, 240
AcvI CACGTG 1 cut(s) 59
AfaI GTAC 2 cut(s) 89, 122
AluBI AGCT 2 cut(s) 46, 362
AluI AGCT 2 cut(s) 46, 362
AlwI GGATC 2 cut(s) 227, 240
ApeKI GCWGC 2 cut(s) 53, 326
AspS9I GGNCC 1 cut(s) 251
AsuHPI GGTGA 2 cut(s) 223, 289
AvaII GGWCC 1 cut(s) 251
BamHI GGATCC 1 cut(s) 232
BbrPI CACGTG 1 cut(s) 59
BbvI GCAGC 2 cut(s) 65, 338
BccI CCATC 2 cut(s) 77, 290
BceAI ACGGC 1 cut(s) 119
BisI GCNGC 2 cut(s) 54, 327
BlsI GCNGC 2 cut(s) 55, 328
Bme18I GGWCC 1 cut(s) 251
BmgT120I GGNCC 1 cut(s) 251
BmiI GGNNCC 2 cut(s) 234, 252
BmrI ACTGGG 1 cut(s) 238
BmuI ACTGGG 1 cut(s) 238
Bpu10I CCTNAGC 1 cut(s) 47
BsaAI YACGTR 1 cut(s) 59
BsaJI CCNNGG 2 cut(s) 237, 294
Bse1I ACTGG 3 cut(s) 233, 344, 350
Bse3DI GCAATG 1 cut(s) 263
BseDI CCNNGG 2 cut(s) 237, 294
BseGI GGATG 3 cut(s) 26, 88, 282
BseMI GCAATG 1 cut(s) 263
BseMII CTCAG 2 cut(s) 61, 144
BseNI ACTGG 3 cut(s) 233, 344, 350
BseXI GCAGC 2 cut(s) 65, 338
BslFI GGGAC 1 cut(s) 264
BsmFI GGGAC 1 cut(s) 264
Bsp143I GATC 2 cut(s) 216, 232
BspACI CCGC 1 cut(s) 324
BspCNI CTCAG 2 cut(s) 60, 143
BspLI GGNNCC 2 cut(s) 234, 252
BspPI GGATC 2 cut(s) 227, 240
BsrDI GCAATG 1 cut(s) 263
BsrI ACTGG 3 cut(s) 233, 344, 350
BssECI CCNNGG 2 cut(s) 237, 294
BssMI GATC 2 cut(s) 216, 232
BssT1I CCWWGG 2 cut(s) 237, 294
Bst4CI ACNGT 2 cut(s) 159, 306
BstAPI GCANNNNNTGC 1 cut(s) 59
BstBAI YACGTR 1 cut(s) 59
BstC8I GCNNGC 1 cut(s) 245
BstDEI CTNAG 3 cut(s) 47, 130, 152
BstEII GGTNACC 1 cut(s) 277
BstF5I GGATG 3 cut(s) 26, 88, 282
BstKTI GATC 2 cut(s) 219, 235
BstMBI GATC 2 cut(s) 216, 232
BstMWI GCNNNNNNNGC 1 cut(s) 59
BstPI GGTNACC 1 cut(s) 277
BstV1I GCAGC 2 cut(s) 65, 338
BstX2I RGATCY 1 cut(s) 232
BstYI RGATCY 1 cut(s) 232
BtsCI GGATG 3 cut(s) 26, 88, 282
Cac8I GCNNGC 1 cut(s) 245
Cfr13I GGNCC 1 cut(s) 251
Csp6I GTAC 2 cut(s) 88, 121
CviAII CATG 2 cut(s) 91, 220
CviJI RGCY 5 cut(s) 46, 109, 134, 343, 362
CviKI_1 RGCY 5 cut(s) 46, 109, 134, 343, 362
CviQI GTAC 2 cut(s) 88, 121
DdeI CTNAG 3 cut(s) 47, 130, 152
DpnI GATC 2 cut(s) 218, 234
DpnII GATC 2 cut(s) 216, 232
Eco130I CCWWGG 2 cut(s) 237, 294
Eco47I GGWCC 1 cut(s) 251
Eco72I CACGTG 1 cut(s) 59
Eco91I GGTNACC 1 cut(s) 277
EcoO65I GGTNACC 1 cut(s) 277
EcoT14I CCWWGG 2 cut(s) 237, 294
ErhI CCWWGG 2 cut(s) 237, 294
FaeI CATG 2 cut(s) 94, 223
FaiI YATR 3 cut(s) 92, 221, 338
FaqI GGGAC 1 cut(s) 264
FatI CATG 2 cut(s) 90, 219
FauI CCCGC 1 cut(s) 331
Fnu4HI GCNGC 2 cut(s) 54, 327
FokI GGATG 3 cut(s) 33, 95, 269
Fsp4HI GCNGC 2 cut(s) 54, 327
GluI GCNGC 2 cut(s) 54, 327
Hin1II CATG 2 cut(s) 94, 223
HinfI GANTC 2 cut(s) 128, 259
HphI GGTGA 2 cut(s) 223, 289
HpyCH4III ACNGT 2 cut(s) 159, 306
HpyCH4IV ACGT 2 cut(s) 58, 144
HpyCH4V TGCA 3 cut(s) 14, 187, 356
HpyF10VI GCNNNNNNNGC 1 cut(s) 59
HpyF3I CTNAG 3 cut(s) 47, 130, 152
HpySE526I ACGT 2 cut(s) 58, 144
Hsp92II CATG 2 cut(s) 94, 223
Kzo9I GATC 2 cut(s) 216, 232
LpnPI CCDG 8 cut(s) 34, 87, 163, 176, 214, 233, 282, 357
Lsp1109I GCAGC 2 cut(s) 65, 338
MaeII ACGT 2 cut(s) 58, 144
MaeIII GTNAC 1 cut(s) 277
MalI GATC 2 cut(s) 218, 234
MboI GATC 2 cut(s) 216, 232
MboII GAAGA 4 cut(s) 140, 226, 302, 311
MflI RGATCY 1 cut(s) 232
MluCI AATT 1 cut(s) 162
MlyI GAGTC 2 cut(s) 122, 253
MnlI CCTC 3 cut(s) 19, 109, 132
MseI TTAA 4 cut(s) 78, 161, 207, 364
MwoI GCNNNNNNNGC 1 cut(s) 59
NdeII GATC 2 cut(s) 216, 232
NlaIII CATG 2 cut(s) 94, 223
NlaIV GGNNCC 2 cut(s) 234, 252
NmuCI GTSAC 1 cut(s) 277
PkrI GCNGC 2 cut(s) 55, 328
PleI GAGTC 2 cut(s) 122, 253
PmaCI CACGTG 1 cut(s) 59
PmlI CACGTG 1 cut(s) 59
PpsI GAGTC 2 cut(s) 122, 253
Ppu21I YACGTR 1 cut(s) 59
PspCI CACGTG 1 cut(s) 59
PspEI GGTNACC 1 cut(s) 277
PspN4I GGNNCC 2 cut(s) 234, 252
PspPI GGNCC 1 cut(s) 251
PsuI RGATCY 1 cut(s) 232
RsaI GTAC 2 cut(s) 89, 122
RsaNI GTAC 2 cut(s) 88, 121
SaqAI TTAA 4 cut(s) 78, 161, 207, 364
SatI GCNGC 2 cut(s) 54, 327
Sau3AI GATC 2 cut(s) 216, 232
Sau96I GGNCC 1 cut(s) 251
SchI GAGTC 2 cut(s) 122, 253
SetI ASST 6 cut(s) 30, 48, 61, 147, 213, 364
SinI GGWCC 1 cut(s) 251
Sse9I AATT 1 cut(s) 162
SsiI CCGC 1 cut(s) 324
StyI CCWWGG 2 cut(s) 237, 294
TaaI ACNGT 2 cut(s) 159, 306
TaiI ACGT 2 cut(s) 61, 147
TasI AATT 1 cut(s) 162
TatI WGTACW 1 cut(s) 120
Tru1I TTAA 4 cut(s) 78, 161, 207, 364
Tru9I TTAA 4 cut(s) 78, 161, 207, 364
TseFI GTSAC 1 cut(s) 277
TseI GCWGC 2 cut(s) 53, 326
Tsp45I GTSAC 1 cut(s) 277
TspDTI ATGAA 1 cut(s) 86
VpaK11BI GGWCC 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.