pycom08g11280

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0028741
Physical Location & Seq
Forward (+)
9569825 .. 9571639
1815 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g11280.1

Sequence Viewer

Length: 1725 bp
ATGGCTGGAGCTTTTATCAGCGATGAGGCTTCTCTCTCTGCTTCGGTCCAGCTGCTTTGCAAAAGGATTGATTCGTGTGAATTGGGTTACTCGTTCGGGCCAACCAAACTTGATAAGTCACTTGTGAATAAACTCAAGTTGGCGCTGCTAACCCTCGATGCAGTGCTCGACGAAGCACATGAGAAGGAATTTCGGGACGCTTATGTAGCAAACTCGCTTGACGAGTTCAGAAAAGCAGTCTCTGATACGAAGGCCCTACTGGATGAGATCGATCTGCATGTGTCCCAGAATAAGATGGAAGCAAAAGATCAGTCCACGATTACAACCCCCGTGAACATCCTCTCTGATTCTTTTCAACGCGTGGATAGAGAGATAGCCGAGTTATCTAAGAGACTAGAATTCCTTGCAAACCAAAAAGATGTGCTCGGTCTTGGAAAAGGTCTTCGCAAGGTTTCACAAGCACCACCAACTTCCTTGTTTGTTGAATCATGTATATATGGTAGAGAACAAGACAGAGATAAGTTAATTAAGCTATTGATATCTGACGACTATGGAAGCAGTAGTCATGTATCTGTCATCACCATCGAAGGAATGGTTGGGATTGGTAAGACAACCCTTGCTCAACTAATTTACAATGATGAATATGTGCCACAGTCCTTTGATCTTCGAGCTTGGGTTTATGTTTCTGAAGATTTCGATGCTACGAGAGTCACTAAAACCATTCTTGAGTCGGTCACTGCAACTGTTTGTGATATTAACGATCTAAATTTCATGCAAGCCAATCTCAAAGACCGAGTGATGGGAAAGAAATTTTTATTTATATTGGATGACCTTTGCAATGAGAATTATCATGTCTGGAAGCTCCTGCAAGCTCCCTTTCAGTACGGGGCCAAGGGAAGCAAGGTCGTGATAACAACACGGAACACAACAGTTGCATCAATTGTCCGCAATCTTCCTACTTACTGTTTGCAACCGCTATCTGGTGAAGATACTTGGCTTTTAATTCTACAACATGCTTTGGGAAACAGAGACCCCATTTCACACCGAAACATTGAAAAACTTGACAAGGAAACTGCAGTGCGTAAGTGCCATGGTTTGCCTTTAATTGCAAAAGCACTTGGGGGTCTCTTAAGGCACACTGTTTTTGAGGAATGGCACCGCATATTAAATAGCAACTTTTGGGAGCTACCCTCGATTGCTCGTGATCTTCCAACCTGCTTACTACAATACATGAGGCAAAAGGGACACGAATCACATAACATTGGCCACTCTACTAGGACGAAGGCCAATTCAGCAGAAAAGATCTTTTTGGTCACCAGCCTCTGCTTAGAAATTGTATCAGCTGCTTGTGATCAGGCTTCCTCGCCAAGCAAGCCCGGCTATGCACTTTCTGGTATGCTCTTGGCTATTGCAGCTGTACTCATTTGCATATGGGAGCTCATTCACAAAGGGAGAGAAGAAAACTTTGTGCGGAGGAAGCAGCAGGGGATACGGTCATGGATTTACTACATGCTTTTGGGTTCTCTCCCTAATATTTACGGCTTACTTGGTGGTATCACTCAGTTTGTTTTTTCAACAGTTCAATATGCTTACGTAATCCAGCGTGCTGAAACTCCGATCAAAATGTCCCTCTTGCCTACTGTCTTTCTTATATGTTTGATTACTATGAGATTAAGCAGGAAACGAATGCAGACCGTAGATAAGACTGCTGAACATACAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
FNIP LRR_8 NB-ARC

Protein Analysis

575

Amino Acids

64.5

Weight (kDa)

7.9

Isoelectric Point (pI)

41.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 39 - 104 5.4e-07 Rx N-terminal domain
NB-ARC PF00931 169 - 339 4.2e-28 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028741)

Species Orthologous Gene IDs
malus_domestica MD00G1172900.v1.1
pyrus_communis pycom08g11280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1223
AccB1I GGYRCC 1 cut(s) 1155
AccII CGCG 1 cut(s) 360
AciI CCGC 4 cut(s) 946, 974, 1159, 1471
AcoI YGGCCR 1 cut(s) 1264
AcsI RAATTY 4 cut(s) 188, 398, 766, 809
AcuI CTGAAG 1 cut(s) 708
AfaI GTAC 2 cut(s) 884, 1419
AfiI CCNNNNNNNGG 2 cut(s) 799, 980
AflII CTTAAG 1 cut(s) 1129
AflIII ACRYGT 1 cut(s) 358
AgsI TTSAA 5 cut(s) 356, 485, 1055, 1575, 1583
AjuI GAANNNNNNNTTGG 2 cut(s) 579, 611
Alw21I GWGCWC 3 cut(s) 168, 426, 1440
Alw26I GTCTC 4 cut(s) 244, 385, 1023, 1130
AlwNI CAGNNNCTG 2 cut(s) 242, 1323
AoxI GGCC 5 cut(s) 98, 252, 888, 1264, 1284
ApeKI GCWGC 5 cut(s) 52, 145, 1343, 1412, 1480
ApoI RAATTY 4 cut(s) 188, 398, 766, 809
ArsI GACNNNNNNTTYG 2 cut(s) 296, 328
AspLEI GCGC 1 cut(s) 145
AspS9I GGNCC 4 cut(s) 46, 98, 253, 888
AsuC2I CCSGG 1 cut(s) 1377
AsuHPI GGTGA 3 cut(s) 571, 995, 1306
AvaII GGWCC 1 cut(s) 46
BalI TGGCCA 1 cut(s) 1266
BanI GGYRCC 1 cut(s) 1155
BanII GRGCYC 1 cut(s) 1440
BauI CACGAG 1 cut(s) 1200
BbsI GAAGAC 1 cut(s) 434
Bbv12I GWGCWC 3 cut(s) 168, 426, 1440
BbvI GCAGC 5 cut(s) 39, 132, 1330, 1424, 1492
BccI CCATC 3 cut(s) 289, 590, 793
BceAI ACGGC 1 cut(s) 1555
BciVI GTATCC 1 cut(s) 1482
BclI TGATCA 1 cut(s) 1351
BcnI CCSGG 1 cut(s) 1377
BcoDI GTCTC 4 cut(s) 244, 385, 1023, 1130
BfaI CTAG 2 cut(s) 395, 1275
BfmI CTRYAG 1 cut(s) 1074
BfoI RGCGCY 1 cut(s) 146
BfrI CTTAAG 1 cut(s) 1129
BfuAI ACCTGC 1 cut(s) 1223
BfuI GTATCC 1 cut(s) 1482
BglII AGATCT 1 cut(s) 1302
BisI GCNGC 5 cut(s) 53, 146, 1344, 1413, 1481
BlsI GCNGC 5 cut(s) 54, 147, 1345, 1414, 1482
Bme1390I CCNGG 1 cut(s) 1377
Bme18I GGWCC 1 cut(s) 46
BmgT120I GGNCC 4 cut(s) 46, 98, 253, 888
BmiI GGNNCC 2 cut(s) 889, 1157
BmrFI CCNGG 1 cut(s) 1377
BmsI GCATC 3 cut(s) 148, 688, 944
BpiI GAAGAC 1 cut(s) 434
BplI GAGNNNNNCTC 2 cut(s) 1175, 1207
BpmI CTGGAG 1 cut(s) 27
BpuEI CTTGAG 2 cut(s) 119, 746
BpuMI CCSGG 1 cut(s) 1377
Bsa29I ATCGAT 1 cut(s) 270
BsaAI YACGTR 1 cut(s) 1594
BsaI GGTCTC 2 cut(s) 1023, 1130
BsaJI CCNNGG 2 cut(s) 891, 1090
Bsc4I CCNNNNNNNGG 2 cut(s) 799, 980
Bse1I ACTGG 1 cut(s) 264
Bse3DI GCAATG 1 cut(s) 844
BseCI ATCGAT 1 cut(s) 270
BseDI CCNNGG 2 cut(s) 891, 1090
BseGI GGATG 3 cut(s) 268, 336, 832
BseLI CCNNNNNNNGG 2 cut(s) 799, 980
BseMI GCAATG 1 cut(s) 844
BseMII CTCAG 1 cut(s) 1574
BseNI ACTGG 1 cut(s) 264
BseXI GCAGC 5 cut(s) 39, 132, 1330, 1424, 1492
Bsh1236I CGCG 1 cut(s) 360
BshFI GGCC 5 cut(s) 100, 254, 890, 1266, 1286
BshNI GGYRCC 1 cut(s) 1155
BshVI ATCGAT 1 cut(s) 270
BsiHKAI GWGCWC 3 cut(s) 168, 426, 1440
BsiSI CCGG 1 cut(s) 1377
BslFI GGGAC 4 cut(s) 209, 268, 1257, 1612
BslI CCNNNNNNNGG 2 cut(s) 799, 980
BsmAI GTCTC 4 cut(s) 244, 385, 1023, 1130
BsmFI GGGAC 4 cut(s) 209, 268, 1257, 1612
BsmI GAATGC 1 cut(s) 1692
BsnI GGCC 5 cut(s) 100, 254, 890, 1266, 1286
Bso31I GGTCTC 2 cut(s) 1023, 1130
Bsp1286I GDGCHC 3 cut(s) 168, 426, 1440
Bsp143I GATC 9 cut(s) 267, 271, 307, 661, 760, 1204, 1302, 1351, 1617
Bsp19I CCATGG 1 cut(s) 1090
BspACI CCGC 4 cut(s) 946, 974, 1159, 1471
BspANI GGCC 5 cut(s) 100, 254, 890, 1266, 1286
BspCNI CTCAG 1 cut(s) 1573
BspDI ATCGAT 1 cut(s) 270
BspFNI CGCG 1 cut(s) 360
BspLI GGNNCC 2 cut(s) 889, 1157
BspMAI CTGCAG 1 cut(s) 1078
BspMI ACCTGC 1 cut(s) 1223
BspT107I GGYRCC 1 cut(s) 1155
BspTI CTTAAG 1 cut(s) 1129
BspTNI GGTCTC 2 cut(s) 1023, 1130
BsrDI GCAATG 1 cut(s) 844
BsrI ACTGG 1 cut(s) 264
BssECI CCNNGG 2 cut(s) 891, 1090
BssMI GATC 9 cut(s) 267, 271, 307, 661, 760, 1204, 1302, 1351, 1617
BssSI CACGAG 1 cut(s) 1200
BssT1I CCWWGG 2 cut(s) 891, 1090
Bst2BI CACGAG 1 cut(s) 1200
Bst4CI ACNGT 9 cut(s) 654, 745, 931, 965, 1141, 1494, 1579, 1642, 1696
BstAFI CTTAAG 1 cut(s) 1129
BstBAI YACGTR 1 cut(s) 1594
BstC8I GCNNGC 4 cut(s) 777, 870, 1373, 1605
BstDEI CTNAG 3 cut(s) 387, 1327, 1560
BstDSI CCRYGG 1 cut(s) 1090
BstEII GGTNACC 1 cut(s) 1312
BstF5I GGATG 3 cut(s) 268, 336, 832
BstFNI CGCG 1 cut(s) 360
BstH2I RGCGCY 1 cut(s) 146
BstHHI GCGC 1 cut(s) 145
BstKTI GATC 9 cut(s) 270, 274, 310, 664, 763, 1207, 1305, 1354, 1620
BstMAI GTCTC 4 cut(s) 244, 385, 1023, 1130
BstMBI GATC 9 cut(s) 267, 271, 307, 661, 760, 1204, 1302, 1351, 1617
BstMWI GCNNNNNNNGC 6 cut(s) 206, 1292, 1372, 1377, 1412, 1477
BstNSI RCATGY 3 cut(s) 281, 1016, 1513
BstPI GGTNACC 1 cut(s) 1312
BstSCI CCNGG 1 cut(s) 1375
BstSFI CTRYAG 1 cut(s) 1074
BstSNI TACGTA 1 cut(s) 1594
BstUI CGCG 1 cut(s) 360
BstV1I GCAGC 5 cut(s) 39, 132, 1330, 1424, 1492
BstV2I GAAGAC 1 cut(s) 434
BstX2I RGATCY 1 cut(s) 1302
BstYI RGATCY 1 cut(s) 1302
Bsu15I ATCGAT 1 cut(s) 270
BsuI GTATCC 1 cut(s) 1482
BsuRI GGCC 5 cut(s) 100, 254, 890, 1266, 1286
BsuTUI ATCGAT 1 cut(s) 270
BtgI CCRYGG 1 cut(s) 1090
BtgZI GCGATG 1 cut(s) 36
BtsCI GGATG 3 cut(s) 268, 336, 832
BtsI GCAGTG 3 cut(s) 168, 735, 1083
BtsIMutI CAGTG 4 cut(s) 168, 735, 1083, 1137
BveI ACCTGC 1 cut(s) 1223
Cac8I GCNNGC 4 cut(s) 777, 870, 1373, 1605
CaiI CAGNNNCTG 2 cut(s) 242, 1323
CfoI GCGC 1 cut(s) 145
Cfr13I GGNCC 4 cut(s) 46, 98, 253, 888
ClaI ATCGAT 1 cut(s) 270
CseI GACGC 1 cut(s) 206
Csp6I GTAC 2 cut(s) 883, 1418
CviQI GTAC 2 cut(s) 883, 1418
DdeI CTNAG 3 cut(s) 387, 1327, 1560
DpnI GATC 9 cut(s) 269, 273, 309, 663, 762, 1206, 1304, 1353, 1619
DpnII GATC 9 cut(s) 267, 271, 307, 661, 760, 1204, 1302, 1351, 1617
EaeI YGGCCR 1 cut(s) 1264
Ecl136II GAGCTC 1 cut(s) 1438
Eco105I TACGTA 1 cut(s) 1594
Eco130I CCWWGG 2 cut(s) 891, 1090
Eco24I GRGCYC 1 cut(s) 1440
Eco31I GGTCTC 2 cut(s) 1023, 1130
Eco32I GATATC 1 cut(s) 540
Eco47I GGWCC 1 cut(s) 46
Eco53kI GAGCTC 1 cut(s) 1438
Eco57I CTGAAG 1 cut(s) 708
Eco91I GGTNACC 1 cut(s) 1312
EcoICRI GAGCTC 1 cut(s) 1438
EcoO109I RGGNCCY 1 cut(s) 253
EcoO65I GGTNACC 1 cut(s) 1312
EcoRI GAATTC 1 cut(s) 398
EcoRV GATATC 1 cut(s) 540
EcoT14I CCWWGG 2 cut(s) 891, 1090
EcoT38I GRGCYC 1 cut(s) 1440
ErhI CCWWGG 2 cut(s) 891, 1090
FaqI GGGAC 4 cut(s) 209, 268, 1257, 1612
FauNDI CATATG 1 cut(s) 1430
FbaI TGATCA 1 cut(s) 1351
Fnu4HI GCNGC 5 cut(s) 53, 146, 1344, 1413, 1481
FokI GGATG 3 cut(s) 275, 323, 839
FriOI GRGCYC 1 cut(s) 1440
Fsp4HI GCNGC 5 cut(s) 53, 146, 1344, 1413, 1481
FspBI CTAG 2 cut(s) 395, 1275
GlaI GCGC 1 cut(s) 144
GluI GCNGC 5 cut(s) 53, 146, 1344, 1413, 1481
GsuI CTGGAG 1 cut(s) 27
HaeII RGCGCY 1 cut(s) 146
HaeIII GGCC 5 cut(s) 100, 254, 890, 1266, 1286
HapII CCGG 1 cut(s) 1377
HgaI GACGC 1 cut(s) 206
HhaI GCGC 1 cut(s) 145
Hin6I GCGC 1 cut(s) 143
HinP1I GCGC 1 cut(s) 143
HinfI GANTC 6 cut(s) 71, 347, 485, 708, 728, 1250
HpaII CCGG 1 cut(s) 1377
HphI GGTGA 3 cut(s) 571, 995, 1306
Hpy166II GTNNAC 2 cut(s) 315, 334
Hpy188I TCNGA 6 cut(s) 230, 244, 346, 544, 688, 1617
Hpy188III TCNNGA 5 cut(s) 194, 725, 856, 907, 1202
Hpy8I GTNNAC 2 cut(s) 315, 334
Hpy99I CGWCG 1 cut(s) 173
HpyAV CCTTC 4 cut(s) 178, 244, 581, 1276
HpyCH4III ACNGT 9 cut(s) 654, 745, 931, 965, 1141, 1494, 1579, 1642, 1696
HpyCH4IV ACGT 1 cut(s) 1593
HpyF10VI GCNNNNNNNGC 6 cut(s) 206, 1292, 1372, 1377, 1412, 1477
HpyF3I CTNAG 3 cut(s) 387, 1327, 1560
HpySE526I ACGT 1 cut(s) 1593
HspAI GCGC 1 cut(s) 143
Ksp22I TGATCA 1 cut(s) 1351
Kzo9I GATC 9 cut(s) 267, 271, 307, 661, 760, 1204, 1302, 1351, 1617
LmnI GCTCC 5 cut(s) 8, 867, 877, 1183, 1435
Lsp1109I GCAGC 5 cut(s) 39, 132, 1330, 1424, 1492
LweI GCATC 3 cut(s) 148, 688, 944
MaeI CTAG 2 cut(s) 395, 1275
MaeII ACGT 1 cut(s) 1593
MaeIII GTNAC 5 cut(s) 86, 117, 709, 733, 1312
MalI GATC 9 cut(s) 269, 273, 309, 663, 762, 1206, 1304, 1353, 1619
MboI GATC 9 cut(s) 267, 271, 307, 661, 760, 1204, 1302, 1351, 1617
MboII GAAGA 7 cut(s) 434, 656, 701, 944, 998, 1199, 1469
MfeI CAATTG 1 cut(s) 939
MflI RGATCY 1 cut(s) 1302
MhlI GDGCHC 3 cut(s) 168, 426, 1440
MlsI TGGCCA 1 cut(s) 1266
MluI ACGCGT 1 cut(s) 358
MluNI TGGCCA 1 cut(s) 1266
MlyI GAGTC 2 cut(s) 717, 737
MmeI TCCRAC 1 cut(s) 1235
Mox20I TGGCCA 1 cut(s) 1266
MscI TGGCCA 1 cut(s) 1266
MseI TTAA 8 cut(s) 524, 528, 756, 1001, 1103, 1130, 1166, 1673
Msp20I TGGCCA 1 cut(s) 1266
MspA1I CMGCKG 3 cut(s) 52, 1343, 1415
MspCI CTTAAG 1 cut(s) 1129
MspI CCGG 1 cut(s) 1377
MspR9I CCNGG 1 cut(s) 1377
MunI CAATTG 1 cut(s) 939
Mva1269I GAATGC 1 cut(s) 1692
MvnI CGCG 1 cut(s) 360
MwoI GCNNNNNNNGC 6 cut(s) 206, 1292, 1372, 1377, 1412, 1477
NciI CCSGG 1 cut(s) 1377
NcoI CCATGG 1 cut(s) 1090
NdeI CATATG 1 cut(s) 1430
NdeII GATC 9 cut(s) 267, 271, 307, 661, 760, 1204, 1302, 1351, 1617
NlaIV GGNNCC 2 cut(s) 889, 1157
NmeAIII GCCGAG 1 cut(s) 403
NmuCI GTSAC 4 cut(s) 117, 709, 733, 1312
NspI RCATGY 3 cut(s) 281, 1016, 1513
PacI TTAATTAA 1 cut(s) 528
PctI GAATGC 1 cut(s) 1692
PfeI GAWTC 4 cut(s) 71, 347, 485, 1250
PkrI GCNGC 5 cut(s) 54, 147, 1345, 1414, 1482
PleI GAGTC 2 cut(s) 716, 736
PpsI GAGTC 2 cut(s) 716, 736
Ppu21I YACGTR 1 cut(s) 1594
Psp124BI GAGCTC 1 cut(s) 1440
PspEI GGTNACC 1 cut(s) 1312
PspN4I GGNNCC 2 cut(s) 889, 1157
PspPI GGNCC 4 cut(s) 46, 98, 253, 888
PstI CTGCAG 1 cut(s) 1078
PstNI CAGNNNCTG 2 cut(s) 242, 1323
PsuI RGATCY 1 cut(s) 1302
PvuII CAGCTG 3 cut(s) 52, 1343, 1415
RsaI GTAC 2 cut(s) 884, 1419
RsaNI GTAC 2 cut(s) 883, 1418
SacI GAGCTC 1 cut(s) 1440
SaqAI TTAA 8 cut(s) 524, 528, 756, 1001, 1103, 1130, 1166, 1673
SatI GCNGC 5 cut(s) 53, 146, 1344, 1413, 1481
Sau3AI GATC 9 cut(s) 267, 271, 307, 661, 760, 1204, 1302, 1351, 1617
Sau96I GGNCC 4 cut(s) 46, 98, 253, 888
SchI GAGTC 2 cut(s) 717, 737
ScrFI CCNGG 1 cut(s) 1377
SduI GDGCHC 3 cut(s) 168, 426, 1440
SfaNI GCATC 3 cut(s) 148, 688, 944
SfcI CTRYAG 1 cut(s) 1074
SinI GGWCC 1 cut(s) 46
SmlI CTYRAG 3 cut(s) 134, 725, 1129
SmoI CTYRAG 3 cut(s) 134, 725, 1129
SnaBI TACGTA 1 cut(s) 1594
SsiI CCGC 4 cut(s) 946, 974, 1159, 1471
SspI AATATT 1 cut(s) 1534
SspMI CTAG 2 cut(s) 395, 1275
SstI GAGCTC 1 cut(s) 1440
StyD4I CCNGG 1 cut(s) 1375
StyI CCWWGG 2 cut(s) 891, 1090
TaaI ACNGT 9 cut(s) 654, 745, 931, 965, 1141, 1494, 1579, 1642, 1696
TaiI ACGT 1 cut(s) 1596
TaqI TCGA 7 cut(s) 156, 168, 270, 585, 667, 696, 1193
TaqII GACCGA 4 cut(s) 34, 416, 721, 807
TatI WGTACW 1 cut(s) 1417
TfiI GAWTC 4 cut(s) 71, 347, 485, 1250
Tru1I TTAA 8 cut(s) 524, 528, 756, 1001, 1103, 1130, 1166, 1673
Tru9I TTAA 8 cut(s) 524, 528, 756, 1001, 1103, 1130, 1166, 1673
TscAI CASTG 4 cut(s) 168, 742, 1083, 1144
TseFI GTSAC 4 cut(s) 117, 709, 733, 1312
TseI GCWGC 5 cut(s) 52, 145, 1343, 1412, 1480
Tsp45I GTSAC 4 cut(s) 117, 709, 733, 1312
TspDTI ATGAA 2 cut(s) 654, 760
TspGWI ACGGA 1 cut(s) 934
TspRI CASTG 4 cut(s) 168, 742, 1083, 1144
Vha464I CTTAAG 1 cut(s) 1129
VpaK11BI GGWCC 1 cut(s) 46
XapI RAATTY 4 cut(s) 188, 398, 766, 809
XceI RCATGY 3 cut(s) 281, 1016, 1513
XcmI CCANNNNNNNNNTGG 1 cut(s) 589
XspI CTAG 2 cut(s) 395, 1275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.