pycom08g14600

UPF0695 membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0028646
Physical Location & Seq
Forward (+)
13909119 .. 13909601
483 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 483 bp
ATGCGACGGATTATATCAGGTTTGTTTCTTGCGGGCTACTCCATCATTTTTAGGCTAGAGACAGCAAGGGGTTTTAGGCATCTTCTTGATAGGAGCTTAGTATGTGGTGTCACTAGCTCTAGAAGGAGCTCGAGGGTTGATAGCTACAAGCGTCACCAGGTAGCTTTAGCAGTATTCTTGCTGATGCTAGGTTCTCTATGGGGTGTGAGTGGAATATTGTTGAGAGAGGAATTTAACAGCGACAGCAGCGAGGTGCAACTGTGGTTGGGTTGCATAGTTGGACCTCTTGGTGTGTGGATCCGGTGGTTCTTAACCTGGCTTAATGGACGTGTATTAGGGATGGCGGGGTTGTTTAAATGGGTTCTATTTGGTACTCTAATTGCCAATGTTTCTGCAGCGTTTGTGTTGGCTGCCCTGTCTACTGTGAAGAAAGCGGGAAGCTCTTGTGATATGATCTCTGTCCGTATCCACATGATTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRCB

Protein Analysis

161

Amino Acids

17.79

Weight (kDa)

10.81

Isoelectric Point (pI)

44.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRCB PF02537 91 - 142 4.3e-06 CrcB-like protein, Camphor Resistance (CrcB)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028646)

Species Orthologous Gene IDs
malus_domestica MD08G1170500.v1.1
pyrus_communis pycom08g14600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 419
AciI CCGC 3 cut(s) 32, 344, 434
AclWI GGATC 2 cut(s) 292, 305
AcsI RAATTY 1 cut(s) 230
AfaI GTAC 1 cut(s) 373
AflIII ACRYGT 1 cut(s) 328
AjiI CACGTC 1 cut(s) 329
AjnI CCWGG 2 cut(s) 156, 314
AluBI AGCT 6 cut(s) 96, 117, 129, 144, 164, 441
AluI AGCT 6 cut(s) 96, 117, 129, 144, 164, 441
Alw21I GWGCWC 1 cut(s) 131
Alw26I GTCTC 1 cut(s) 53
AlwI GGATC 2 cut(s) 292, 305
Ama87I CYCGRG 1 cut(s) 130
ApeKI GCWGC 3 cut(s) 246, 395, 410
ApoI RAATTY 1 cut(s) 230
AspS9I GGNCC 1 cut(s) 281
AsuHPI GGTGA 1 cut(s) 146
AvaI CYCGRG 1 cut(s) 130
AvaII GGWCC 1 cut(s) 281
BamHI GGATCC 1 cut(s) 297
BanII GRGCYC 1 cut(s) 131
Bbv12I GWGCWC 1 cut(s) 131
BbvI GCAGC 3 cut(s) 258, 397, 407
BccI CCATC 2 cut(s) 50, 334
BciT130I CCWGG 2 cut(s) 158, 316
BciVI GTATCC 1 cut(s) 476
BcoDI GTCTC 1 cut(s) 53
BfaI CTAG 4 cut(s) 56, 114, 120, 188
BfmI CTRYAG 1 cut(s) 393
BfuI GTATCC 1 cut(s) 476
BisI GCNGC 3 cut(s) 247, 396, 411
BlsI GCNGC 3 cut(s) 248, 397, 412
Bme1390I CCNGG 2 cut(s) 158, 316
Bme18I GGWCC 1 cut(s) 281
BmeT110I CYCGRG 1 cut(s) 130
BmgBI CACGTC 1 cut(s) 329
BmgT120I GGNCC 1 cut(s) 281
BmiI GGNNCC 1 cut(s) 299
BmrFI CCNGG 2 cut(s) 158, 316
BmsI GCATC 2 cut(s) 88, 174
BsaWI WCCGGW 1 cut(s) 300
BseBI CCWGG 2 cut(s) 158, 316
BseGI GGATG 1 cut(s) 345
BseXI GCAGC 3 cut(s) 258, 397, 407
BsiHKAI GWGCWC 1 cut(s) 131
BsiHKCI CYCGRG 1 cut(s) 130
BsiSI CCGG 1 cut(s) 301
BsmAI GTCTC 1 cut(s) 53
BsoBI CYCGRG 1 cut(s) 130
Bsp1286I GDGCHC 1 cut(s) 131
Bsp143I GATC 2 cut(s) 297, 453
BspACI CCGC 3 cut(s) 32, 344, 434
BspLI GGNNCC 1 cut(s) 299
BspMAI CTGCAG 1 cut(s) 397
BspPI GGATC 2 cut(s) 292, 305
BssMI GATC 2 cut(s) 297, 453
Bst2UI CCWGG 2 cut(s) 158, 316
Bst4CI ACNGT 2 cut(s) 261, 424
BstC8I GCNNGC 1 cut(s) 34
BstDEI CTNAG 1 cut(s) 97
BstF5I GGATG 1 cut(s) 345
BstKTI GATC 2 cut(s) 300, 456
BstMAI GTCTC 1 cut(s) 53
BstMBI GATC 2 cut(s) 297, 453
BstMWI GCNNNNNNNGC 1 cut(s) 246
BstNI CCWGG 2 cut(s) 158, 316
BstSCI CCNGG 2 cut(s) 156, 314
BstSFI CTRYAG 1 cut(s) 393
BstV1I GCAGC 3 cut(s) 258, 397, 407
BstX2I RGATCY 1 cut(s) 297
BstYI RGATCY 1 cut(s) 297
BsuI GTATCC 1 cut(s) 476
BtrI CACGTC 1 cut(s) 329
BtsCI GGATG 1 cut(s) 345
Cac8I GCNNGC 1 cut(s) 34
Cfr13I GGNCC 1 cut(s) 281
CseI GACGC 1 cut(s) 140
CsiI ACCWGGT 1 cut(s) 156
Csp6I GTAC 1 cut(s) 372
CviAII CATG 1 cut(s) 472
CviQI GTAC 1 cut(s) 372
DdeI CTNAG 1 cut(s) 97
DpnI GATC 2 cut(s) 299, 455
DpnII GATC 2 cut(s) 297, 453
DraI TTTAAA 1 cut(s) 355
Ecl136II GAGCTC 1 cut(s) 129
Eco24I GRGCYC 1 cut(s) 131
Eco47I GGWCC 1 cut(s) 281
Eco53kI GAGCTC 1 cut(s) 129
Eco88I CYCGRG 1 cut(s) 130
EcoICRI GAGCTC 1 cut(s) 129
EcoRII CCWGG 2 cut(s) 156, 314
EcoT38I GRGCYC 1 cut(s) 131
FaeI CATG 1 cut(s) 475
FaiI YATR 6 cut(s) 14, 103, 199, 275, 452, 473
FatI CATG 1 cut(s) 471
FauI CCCGC 3 cut(s) 25, 337, 427
FblI GTMKAC 1 cut(s) 419
Fnu4HI GCNGC 3 cut(s) 247, 396, 411
FokI GGATG 1 cut(s) 352
FriOI GRGCYC 1 cut(s) 131
Fsp4HI GCNGC 3 cut(s) 247, 396, 411
FspBI CTAG 4 cut(s) 56, 114, 120, 188
GluI GCNGC 3 cut(s) 247, 396, 411
HapII CCGG 1 cut(s) 301
HgaI GACGC 1 cut(s) 140
Hin1II CATG 1 cut(s) 475
HpaII CCGG 1 cut(s) 301
HphI GGTGA 1 cut(s) 146
Hpy166II GTNNAC 1 cut(s) 420
Hpy188III TCNNGA 2 cut(s) 86, 120
Hpy8I GTNNAC 1 cut(s) 420
Hpy99I CGWCG 1 cut(s) 9
HpyAV CCTTC 1 cut(s) 117
HpyCH4III ACNGT 2 cut(s) 261, 424
HpyCH4IV ACGT 1 cut(s) 328
HpyCH4V TGCA 3 cut(s) 256, 273, 395
HpyF10VI GCNNNNNNNGC 1 cut(s) 246
HpyF3I CTNAG 1 cut(s) 97
HpySE526I ACGT 1 cut(s) 328
Hsp92II CATG 1 cut(s) 475
Kzo9I GATC 2 cut(s) 297, 453
LmnI GCTCC 2 cut(s) 93, 126
LpnPI CCDG 7 cut(s) 3, 143, 170, 301, 314, 328, 428
Lsp1109I GCAGC 3 cut(s) 258, 397, 407
LweI GCATC 2 cut(s) 88, 174
MabI ACCWGGT 1 cut(s) 156
MaeI CTAG 4 cut(s) 56, 114, 120, 188
MaeII ACGT 1 cut(s) 328
MaeIII GTNAC 2 cut(s) 109, 152
MalI GATC 2 cut(s) 299, 455
MboI GATC 2 cut(s) 297, 453
MboII GAAGA 2 cut(s) 74, 439
MflI RGATCY 1 cut(s) 297
MhlI GDGCHC 1 cut(s) 131
MluCI AATT 2 cut(s) 230, 378
MmeI TCCRAC 1 cut(s) 259
MnlI CCTC 4 cut(s) 126, 220, 244, 294
MseI TTAA 4 cut(s) 234, 311, 321, 354
MspI CCGG 1 cut(s) 301
MspR9I CCNGG 2 cut(s) 158, 316
MvaI CCWGG 2 cut(s) 158, 316
MwoI GCNNNNNNNGC 1 cut(s) 246
NdeII GATC 2 cut(s) 297, 453
NlaIII CATG 1 cut(s) 475
NlaIV GGNNCC 1 cut(s) 299
NmuCI GTSAC 2 cut(s) 109, 152
PaeR7I CTCGAG 1 cut(s) 130
PkrI GCNGC 3 cut(s) 248, 397, 412
Psp124BI GAGCTC 1 cut(s) 131
Psp6I CCWGG 2 cut(s) 156, 314
PspGI CCWGG 2 cut(s) 156, 314
PspN4I GGNNCC 1 cut(s) 299
PspPI GGNCC 1 cut(s) 281
PspXI VCTCGAGB 1 cut(s) 130
PstI CTGCAG 1 cut(s) 397
PsuI RGATCY 1 cut(s) 297
RsaI GTAC 1 cut(s) 373
RsaNI GTAC 1 cut(s) 372
SacI GAGCTC 1 cut(s) 131
SaqAI TTAA 4 cut(s) 234, 311, 321, 354
SatI GCNGC 3 cut(s) 247, 396, 411
Sau3AI GATC 2 cut(s) 297, 453
Sau96I GGNCC 1 cut(s) 281
ScrFI CCNGG 2 cut(s) 158, 316
SduI GDGCHC 1 cut(s) 131
SexAI ACCWGGT 1 cut(s) 156
SfaNI GCATC 2 cut(s) 88, 174
SfcI CTRYAG 1 cut(s) 393
Sfr274I CTCGAG 1 cut(s) 130
SinI GGWCC 1 cut(s) 281
SlaI CTCGAG 1 cut(s) 130
SmlI CTYRAG 1 cut(s) 130
SmoI CTYRAG 1 cut(s) 130
Sse9I AATT 2 cut(s) 230, 378
SsiI CCGC 3 cut(s) 32, 344, 434
SspI AATATT 1 cut(s) 216
SspMI CTAG 4 cut(s) 56, 114, 120, 188
SstI GAGCTC 1 cut(s) 131
StyD4I CCNGG 2 cut(s) 156, 314
TaaI ACNGT 2 cut(s) 261, 424
TaiI ACGT 1 cut(s) 331
TaqI TCGA 1 cut(s) 131
TasI AATT 2 cut(s) 230, 378
Tru1I TTAA 4 cut(s) 234, 311, 321, 354
Tru9I TTAA 4 cut(s) 234, 311, 321, 354
TseFI GTSAC 2 cut(s) 109, 152
TseI GCWGC 3 cut(s) 246, 395, 410
Tsp45I GTSAC 2 cut(s) 109, 152
TspGWI ACGGA 2 cut(s) 22, 452
VpaK11BI GGWCC 1 cut(s) 281
XapI RAATTY 1 cut(s) 230
XbaI TCTAGA 1 cut(s) 119
XhoI CTCGAG 1 cut(s) 130
XmiI GTMKAC 1 cut(s) 419
XspI CTAG 4 cut(s) 56, 114, 120, 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.