pycom08g17010

Mechanosensitive ion channel protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0028381
Physical Location & Seq
Reverse (-)
17203260 .. 17205636
2377 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 780 bp
ATGCCTCATGCGGCTGCTACGAGGTTTTTGTCTGATTGGAGAAGTCACAATAATCGTGGATGCCGTAATCCAGATGCTTGTCTTGTAGGAAAGGGTCGAGTGCATATGGTTAGCGTCAATCTTCCGTCGCATGGTTTGGGTGCCTGCAGTCTCCATCTTTTGAGCAGTGTACGTGGCCCAATAGGTCCAGTTTCTTCCAGATGCAATGTCTTCGTGTGCAGATCTGTTTTAGGTCCAGGTGGAGGAAACGGGGCTCCTGTTCTGAAATCTGCTACTCTTGTTTTGACAAGGTCCTATGGTACTTTACGTGGAAGCCCTGCTTTACCTAAATTGATCCCAGCAGTGGCTATCATTGCTTTTGCTGTTTGGGGTATTGGGCCACTATTGCGTCTCGGCAGGGTTATTTTTCTTCGACGGAATGATAATACTTGGATGAAAAGTGGGTCACACTATGTCATGACTTCTTACCTTCAGCCCTTGCTTCTCTGGACTGGAGCGACACTTTTATGCAGAGCATTGGATCCTGTAGTCCTACCTTCAGAAGCTAGCCAGGCTGTCAAGCAACGTCTTATAAATTTTACACAATCATTATCTACAGTGCTGACATTTGCCTATTGTTTGTCAAGCTTAATTCAACAAGCACAGAAGTTCTTTATGGAGACAAGTGACCCAAGAAAATGGGCTTCAATTTCGCTGGAAAGGCGGTTTATTCTGCAGTTTGGGTTGCTGCGGTATCATTGTTCATGGAATTGCTGGGATTCTCAACCCAGAAATGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MS_channel

Protein Analysis

260

Amino Acids

28.46

Weight (kDa)

10.43

Isoelectric Point (pI)

49.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MSL2_3 PF25237 150 - 232 2.9e-36 Mechanosensitive channel protein 2/3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028381)

Species Orthologous Gene IDs
malus_domestica MD15G1385200.v1.1
pyrus_communis pycom08g17010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 572
AccB1I GGYRCC 1 cut(s) 140
AciI CCGC 3 cut(s) 11, 703, 730
AclWI GGATC 3 cut(s) 328, 515, 528
AcsI RAATTY 1 cut(s) 574
AcuI CTGAAG 2 cut(s) 455, 522
AfaI GTAC 2 cut(s) 171, 301
AfiI CCNNNNNNNGG 5 cut(s) 131, 242, 343, 773, 774
AgsI TTSAA 2 cut(s) 635, 687
AjnI CCWGG 2 cut(s) 235, 549
AluBI AGCT 2 cut(s) 545, 627
AluI AGCT 2 cut(s) 545, 627
Alw26I GTCTC 3 cut(s) 155, 395, 653
AlwI GGATC 3 cut(s) 328, 515, 528
AoxI GGCC 2 cut(s) 175, 377
ApeKI GCWGC 2 cut(s) 14, 727
ApoI RAATTY 1 cut(s) 574
AspS9I GGNCC 5 cut(s) 176, 185, 233, 291, 377
AsuNHI GCTAGC 1 cut(s) 545
AvaII GGWCC 3 cut(s) 185, 233, 291
BamHI GGATCC 1 cut(s) 520
BanI GGYRCC 1 cut(s) 140
BanII GRGCYC 1 cut(s) 256
BbsI GAAGAC 1 cut(s) 202
BbvI GCAGC 1 cut(s) 714
BccI CCATC 1 cut(s) 162
BceAI ACGGC 1 cut(s) 48
BcgI CGANNNNNNTGC 2 cut(s) 193, 227
BciT130I CCWGG 2 cut(s) 237, 551
BcoDI GTCTC 3 cut(s) 155, 395, 653
BfaI CTAG 1 cut(s) 546
BfmI CTRYAG 4 cut(s) 145, 525, 594, 713
BglII AGATCT 1 cut(s) 221
BisI GCNGC 3 cut(s) 12, 15, 728
BlsI GCNGC 3 cut(s) 13, 16, 729
Bme1390I CCNGG 2 cut(s) 237, 551
Bme18I GGWCC 3 cut(s) 185, 233, 291
BmgT120I GGNCC 5 cut(s) 176, 185, 233, 291, 377
BmiI GGNNCC 3 cut(s) 142, 255, 522
BmrFI CCNGG 2 cut(s) 237, 551
BmsI GCATC 3 cut(s) 50, 64, 191
BmtI GCTAGC 1 cut(s) 549
BpiI GAAGAC 1 cut(s) 202
BpmI CTGGAG 1 cut(s) 513
BsaAI YACGTR 2 cut(s) 173, 308
Bsc4I CCNNNNNNNGG 5 cut(s) 131, 242, 343, 773, 774
Bse1I ACTGG 2 cut(s) 188, 496
Bse3DI GCAATG 2 cut(s) 211, 351
BseBI CCWGG 2 cut(s) 237, 551
BseGI GGATG 2 cut(s) 65, 438
BseLI CCNNNNNNNGG 5 cut(s) 131, 242, 343, 773, 774
BseMI GCAATG 2 cut(s) 211, 351
BseNI ACTGG 2 cut(s) 188, 496
BseXI GCAGC 1 cut(s) 714
BseYI CCCAGC 2 cut(s) 337, 753
BsgI GTGCAG 1 cut(s) 238
BshFI GGCC 2 cut(s) 177, 379
BshNI GGYRCC 1 cut(s) 140
BslI CCNNNNNNNGG 5 cut(s) 131, 242, 343, 773, 774
BsmAI GTCTC 3 cut(s) 155, 395, 653
BsmBI CGTCTC 1 cut(s) 395
BsnI GGCC 2 cut(s) 177, 379
Bsp1286I GDGCHC 1 cut(s) 256
Bsp143I GATC 3 cut(s) 221, 333, 520
BspACI CCGC 3 cut(s) 11, 703, 730
BspANI GGCC 2 cut(s) 177, 379
BspHI TCATGA 1 cut(s) 456
BspLI GGNNCC 3 cut(s) 142, 255, 522
BspMAI CTGCAG 2 cut(s) 149, 717
BspOI GCTAGC 1 cut(s) 549
BspPI GGATC 3 cut(s) 328, 515, 528
BspT107I GGYRCC 1 cut(s) 140
BsrDI GCAATG 2 cut(s) 211, 351
BsrI ACTGG 2 cut(s) 188, 496
BssMI GATC 3 cut(s) 221, 333, 520
Bst2UI CCWGG 2 cut(s) 237, 551
Bst4CI ACNGT 1 cut(s) 598
BstBAI YACGTR 2 cut(s) 173, 308
BstC8I GCNNGC 2 cut(s) 145, 547
BstF5I GGATG 2 cut(s) 65, 438
BstKTI GATC 3 cut(s) 224, 336, 523
BstMAI GTCTC 3 cut(s) 155, 395, 653
BstMBI GATC 3 cut(s) 221, 333, 520
BstMWI GCNNNNNNNGC 4 cut(s) 353, 385, 551, 700
BstNI CCWGG 2 cut(s) 237, 551
BstSCI CCNGG 2 cut(s) 235, 549
BstSFI CTRYAG 4 cut(s) 145, 525, 594, 713
BstV1I GCAGC 1 cut(s) 714
BstV2I GAAGAC 1 cut(s) 202
BstX2I RGATCY 2 cut(s) 221, 520
BstXI CCANNNNNNTGG 1 cut(s) 678
BstYI RGATCY 2 cut(s) 221, 520
BsuRI GGCC 2 cut(s) 177, 379
BtsCI GGATG 2 cut(s) 65, 438
BtsI GCAGTG 2 cut(s) 172, 348
BtsIMutI CAGTG 3 cut(s) 172, 348, 603
Cac8I GCNNGC 2 cut(s) 145, 547
CciI TCATGA 1 cut(s) 456
Cfr13I GGNCC 5 cut(s) 176, 185, 233, 291, 377
CseI GACGC 2 cut(s) 103, 377
Csp6I GTAC 2 cut(s) 170, 300
CspCI CAANNNNNGTGG 2 cut(s) 37, 72
CviAII CATG 4 cut(s) 8, 131, 457, 744
CviQI GTAC 2 cut(s) 170, 300
DpnI GATC 3 cut(s) 223, 335, 522
DpnII GATC 3 cut(s) 221, 333, 520
Eco24I GRGCYC 1 cut(s) 256
Eco47I GGWCC 3 cut(s) 185, 233, 291
Eco57I CTGAAG 2 cut(s) 455, 522
EcoO109I RGGNCCY 1 cut(s) 291
EcoRII CCWGG 2 cut(s) 235, 549
EcoT38I GRGCYC 1 cut(s) 256
Esp3I CGTCTC 1 cut(s) 395
FaeI CATG 4 cut(s) 11, 134, 460, 747
FalI AAGNNNNNCTT 2 cut(s) 304, 336
FatI CATG 4 cut(s) 7, 130, 456, 743
FauNDI CATATG 1 cut(s) 105
Fnu4HI GCNGC 3 cut(s) 12, 15, 728
FokI GGATG 2 cut(s) 72, 445
FriOI GRGCYC 1 cut(s) 256
Fsp4HI GCNGC 3 cut(s) 12, 15, 728
FspBI CTAG 1 cut(s) 546
GluI GCNGC 3 cut(s) 12, 15, 728
GsaI CCCAGC 2 cut(s) 341, 757
GsuI CTGGAG 1 cut(s) 513
HaeIII GGCC 2 cut(s) 177, 379
HgaI GACGC 2 cut(s) 103, 377
Hin1II CATG 4 cut(s) 11, 134, 460, 747
HindIII AAGCTT 1 cut(s) 625
HinfI GANTC 1 cut(s) 758
Hpy166II GTNNAC 1 cut(s) 170
Hpy188I TCNGA 3 cut(s) 34, 264, 541
Hpy188III TCNNGA 4 cut(s) 71, 198, 457, 487
Hpy8I GTNNAC 1 cut(s) 170
Hpy99I CGWCG 2 cut(s) 130, 417
HpyAV CCTTC 2 cut(s) 479, 546
HpyCH4III ACNGT 1 cut(s) 598
HpyCH4IV ACGT 3 cut(s) 172, 307, 565
HpyCH4V TGCA 6 cut(s) 103, 147, 204, 219, 510, 715
HpyF10VI GCNNNNNNNGC 4 cut(s) 353, 385, 551, 700
HpySE526I ACGT 3 cut(s) 172, 307, 565
Hsp92II CATG 4 cut(s) 11, 134, 460, 747
Kzo9I GATC 3 cut(s) 221, 333, 520
LmnI GCTCC 2 cut(s) 259, 494
Lsp1109I GCAGC 1 cut(s) 714
LweI GCATC 3 cut(s) 50, 64, 191
MaeI CTAG 1 cut(s) 546
MaeII ACGT 3 cut(s) 172, 307, 565
MaeIII GTNAC 3 cut(s) 44, 444, 665
MalI GATC 3 cut(s) 223, 335, 522
MboI GATC 3 cut(s) 221, 333, 520
MboII GAAGA 4 cut(s) 113, 186, 202, 401
MflI RGATCY 2 cut(s) 221, 520
MhlI GDGCHC 1 cut(s) 256
MluCI AATT 5 cut(s) 329, 574, 630, 687, 748
MnlI CCTC 3 cut(s) 15, 15, 236
MseI TTAA 1 cut(s) 629
MslI CAYNNNNRTG 1 cut(s) 505
MspR9I CCNGG 2 cut(s) 237, 551
MvaI CCWGG 2 cut(s) 237, 551
MwoI GCNNNNNNNGC 4 cut(s) 353, 385, 551, 700
NdeI CATATG 1 cut(s) 105
NdeII GATC 3 cut(s) 221, 333, 520
NheI GCTAGC 1 cut(s) 545
NlaIII CATG 4 cut(s) 11, 134, 460, 747
NlaIV GGNNCC 3 cut(s) 142, 255, 522
NmeAIII GCCGAG 1 cut(s) 372
NmuCI GTSAC 3 cut(s) 44, 444, 665
PagI TCATGA 1 cut(s) 456
PcsI WCGNNNNNNNCGW 1 cut(s) 61
PfeI GAWTC 1 cut(s) 758
PflFI GACNNNGTC 1 cut(s) 289
PkrI GCNGC 3 cut(s) 13, 16, 729
Ppu21I YACGTR 2 cut(s) 173, 308
PpuMI RGGWCCY 1 cut(s) 291
PsiI TTATAA 1 cut(s) 572
Psp5II RGGWCCY 1 cut(s) 291
Psp6I CCWGG 2 cut(s) 235, 549
PspFI CCCAGC 2 cut(s) 337, 753
PspGI CCWGG 2 cut(s) 235, 549
PspN4I GGNNCC 3 cut(s) 142, 255, 522
PspPI GGNCC 5 cut(s) 176, 185, 233, 291, 377
PspPPI RGGWCCY 1 cut(s) 291
PstI CTGCAG 2 cut(s) 149, 717
PsuI RGATCY 2 cut(s) 221, 520
PsyI GACNNNGTC 1 cut(s) 289
RsaI GTAC 2 cut(s) 171, 301
RsaNI GTAC 2 cut(s) 170, 300
RseI CAYNNNNRTG 1 cut(s) 505
SaqAI TTAA 1 cut(s) 629
SatI GCNGC 3 cut(s) 12, 15, 728
Sau3AI GATC 3 cut(s) 221, 333, 520
Sau96I GGNCC 5 cut(s) 176, 185, 233, 291, 377
ScrFI CCNGG 2 cut(s) 237, 551
SduI GDGCHC 1 cut(s) 256
SfaNI GCATC 3 cut(s) 50, 64, 191
SfcI CTRYAG 4 cut(s) 145, 525, 594, 713
SinI GGWCC 3 cut(s) 185, 233, 291
SmiMI CAYNNNNRTG 1 cut(s) 505
Sse9I AATT 5 cut(s) 329, 574, 630, 687, 748
SsiI CCGC 3 cut(s) 11, 703, 730
SspMI CTAG 1 cut(s) 546
StyD4I CCNGG 2 cut(s) 235, 549
TaaI ACNGT 1 cut(s) 598
TaiI ACGT 3 cut(s) 175, 310, 568
TaqI TCGA 2 cut(s) 97, 412
TasI AATT 5 cut(s) 329, 574, 630, 687, 748
TauI GCSGC 1 cut(s) 14
TfiI GAWTC 1 cut(s) 758
Tru1I TTAA 1 cut(s) 629
Tru9I TTAA 1 cut(s) 629
TscAI CASTG 3 cut(s) 172, 348, 603
TseFI GTSAC 3 cut(s) 44, 444, 665
TseI GCWGC 2 cut(s) 14, 727
Tsp45I GTSAC 3 cut(s) 44, 444, 665
TspDTI ATGAA 2 cut(s) 449, 732
TspGWI ACGGA 2 cut(s) 114, 430
TspRI CASTG 3 cut(s) 172, 348, 603
Tth111I GACNNNGTC 1 cut(s) 289
VpaK11BI GGWCC 3 cut(s) 185, 233, 291
XapI RAATTY 1 cut(s) 574
XspI CTAG 1 cut(s) 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.