pycom08g18320

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0029138
Physical Location & Seq
Forward (+)
18559808 .. 18563655
3848 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1434 bp
ATGGGAGGGGGAGAGCATGAAATCGCTTTAGAGGAGGCACTAATATCTTCCACGTTGAGAAAGCAAAGGCTGCAGGAAATGGAAGGGAAATGGACGGAGTTCCTGAAAGAGCTTAAGAAGGTGAGCTACATGGCAGCGCCGATGGTGGCTGTCATGGTGATGCAGTACCTTATACAGGTTGTTTCCGTGATGATGGTTGGACATGTCGATCAGCTCTCTCTTTCCGGGGTCTCCATCGCCACCTCTTTCACTAGTGTTACTGGCTTCAGTCTTCTTTTTGGTCTAGCTGGTGCACTGGAGACTCTCTGTGGACAAGCATATGGGGCACAGCAATATCAAAAGCTTGGAATTCATACTTACACCTCCATCCTATGTCTCTGTCTCGTCTGTCTCCCAATATCCCTCCTATGGGTATTCACAGACAAACTTCTAAATTTGATAGTCTGTCTCATCCCTAATCTTTTCTCTTACGCCATTCTTCAATCGCTCATCCGGTATTTCCAGACGCAGAGTTTGATTCTTCCGATGTTGTATAGCTCAGGTGCAACTCTGTGTTTGCATGTACCTCTTTGTTGGGCTTTCACCCTTAAACTGAACATAGGAAGTGTTGGAGCAGCATTAGCAATAACCTTCTCTTACTGGTTTAATGTGATTGTACTCGGGTTTTACATGAAGTATTCGGTGGCATGTGAAAAGACTCGTGCTCGTGTGCTTTCAGTAGAAGTCATTCGAAATGTTAAAGAATTCTTCTGTTTTGCTACTCCATCAGCTGCAATGGCTTGTCTTGAATGGTGGTCCTATGAGGTGCTCATTTTGCTTTCTGGGCTATTAGCAAATCCGAAACCGGAAGCATCAGTGCTTTCTATATGCTTTACAATTACTTATTTGCACTACTACATACCATATGCTTTTGGAGCCACAGCAAGCACTAGGGTTTCAAATGAGTTAGGAGCTGGGAATCCTCGCGCTGCTAAAGTGACAGTCTGTGCTATTACGGTTCTTGCAATCGCGGAAATGGTTGTTGTAAGCACAACTCTTTTCTCTTACCGCCATAATCTCGGATATGCATTCAGCAAAGACAACGAGGTTGTGGAGCGTATAGCAGATATGGGTCTTCTTATTAGTCTCTCCATTGTTATGGATGGGTTACAAGCACTACTTTCAGGGGTTGCTAGAGGAACAGGATGGCAGGAGATAGGAGCTTATGTAAATCTGGGGGCATATTATTTGGTTGGCACTCCGGTGGGTGTAGTGCTTGCCTTTGTTGTACACTTGAGAGCCAAGGGCCTATGGATTGGATTATTGACAGGGTCTACTGTTCAGGCTCTCCTGCTTGCTCTTAAAACTGGTCTCACAAATTGGCAAAAGCAGTCTTCACTTGCAAGAGAGAGGATGCTGAAAGAGGTCGTACCTATAGTGGAATCAGTTGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

478

Amino Acids

52.16

Weight (kDa)

8.35

Isoelectric Point (pI)

28.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 47 - 147 3.3e-22 MatE
MatE PF01554 255 - 417 7.3e-27 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029138)

Species Orthologous Gene IDs
prunus_persica Prupe.1G545500_v2.0.a1
pyrus_communis pycom08g18320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1313
AccII CGCG 2 cut(s) 966, 1010
AciI CCGC 2 cut(s) 1010, 1048
AcsI RAATTY 3 cut(s) 348, 433, 743
AcuI CTGAAG 1 cut(s) 250
AfaI GTAC 5 cut(s) 167, 564, 657, 1269, 1410
AfiI CCNNNNNNNGG 3 cut(s) 175, 408, 409
AflII CTTAAG 1 cut(s) 113
AflIII ACRYGT 1 cut(s) 202
AgsI TTSAA 3 cut(s) 482, 788, 939
AhlI ACTAGT 1 cut(s) 251
AleI CACNNNNGTG 1 cut(s) 1241
AluBI AGCT 9 cut(s) 112, 126, 214, 287, 343, 537, 770, 953, 1202
AluI AGCT 9 cut(s) 112, 126, 214, 287, 343, 537, 770, 953, 1202
Alw21I GWGCWC 3 cut(s) 295, 706, 810
Alw26I GTCTC 8 cut(s) 235, 293, 380, 386, 395, 452, 1130, 1355
Alw44I GTGCAC 1 cut(s) 291
Ama87I CYCGRG 1 cut(s) 659
AoxI GGCC 1 cut(s) 1285
ApaLI GTGCAC 1 cut(s) 291
ApeKI GCWGC 5 cut(s) 70, 134, 614, 770, 968
ApoI RAATTY 3 cut(s) 348, 433, 743
ArsI GACNNNNNNTTYG 2 cut(s) 496, 528
Asp700I GAANNNNTTC 1 cut(s) 726
AspLEI GCGC 2 cut(s) 139, 968
AspS9I GGNCC 2 cut(s) 795, 1285
AsuC2I CCSGG 1 cut(s) 226
AsuHPI GGTGA 3 cut(s) 133, 169, 574
AsuII TTCGAA 1 cut(s) 730
AvaI CYCGRG 1 cut(s) 659
AvaII GGWCC 1 cut(s) 795
BaeGI GKGCMC 2 cut(s) 295, 328
BarI GAAGNNNNNNTAC 2 cut(s) 110, 142
BauI CACGAG 2 cut(s) 699, 705
BbsI GAAGAC 3 cut(s) 263, 1106, 1365
Bbv12I GWGCWC 3 cut(s) 295, 706, 810
BbvI GCAGC 5 cut(s) 57, 146, 626, 757, 955
BccI CCATC 7 cut(s) 136, 187, 242, 374, 772, 1136, 1179
BcnI CCSGG 1 cut(s) 226
BcoDI GTCTC 8 cut(s) 235, 293, 380, 386, 395, 452, 1130, 1355
BcuI ACTAGT 1 cut(s) 251
BfaI CTAG 4 cut(s) 252, 284, 930, 1173
BfmI CTRYAG 2 cut(s) 71, 1413
BfoI RGCGCY 1 cut(s) 140
BfrI CTTAAG 1 cut(s) 113
BisI GCNGC 5 cut(s) 71, 135, 615, 771, 969
BlsI GCNGC 5 cut(s) 72, 136, 616, 772, 970
Bme1390I CCNGG 1 cut(s) 226
Bme18I GGWCC 1 cut(s) 795
BmeT110I CYCGRG 1 cut(s) 659
BmgT120I GGNCC 2 cut(s) 795, 1285
BmiI GGNNCC 1 cut(s) 916
BmrFI CCNGG 1 cut(s) 226
BmsI GCATC 3 cut(s) 150, 860, 1383
BpiI GAAGAC 3 cut(s) 263, 1106, 1365
BpmI CTGGAG 1 cut(s) 317
Bpu10I CCTNAGC 1 cut(s) 538
Bpu14I TTCGAA 1 cut(s) 730
BpuEI CTTGAG 1 cut(s) 1294
BpuMI CCSGG 1 cut(s) 226
BsaI GGTCTC 2 cut(s) 235, 1355
BsaJI CCNNGG 2 cut(s) 225, 1281
BsaWI WCCGGW 3 cut(s) 492, 844, 1240
BsaXI ACNNNNNCTCC 2 cut(s) 1191, 1221
Bsc4I CCNNNNNNNGG 3 cut(s) 175, 408, 409
Bse1I ACTGG 4 cut(s) 265, 300, 644, 1351
Bse3DI GCAATG 1 cut(s) 780
BseDI CCNNGG 2 cut(s) 225, 1281
BseGI GGATG 6 cut(s) 366, 450, 489, 1147, 1190, 1398
BseLI CCNNNNNNNGG 3 cut(s) 175, 408, 409
BseMI GCAATG 1 cut(s) 780
BseMII CTCAG 1 cut(s) 552
BseNI ACTGG 4 cut(s) 265, 300, 644, 1351
BseRI GAGGAG 1 cut(s) 47
BseSI GKGCMC 2 cut(s) 295, 328
BseXI GCAGC 5 cut(s) 57, 146, 626, 757, 955
BseYI CCCAGC 1 cut(s) 953
Bsh1236I CGCG 2 cut(s) 966, 1010
BshFI GGCC 1 cut(s) 1287
BsiHKAI GWGCWC 3 cut(s) 295, 706, 810
BsiHKCI CYCGRG 1 cut(s) 659
BsiSI CCGG 4 cut(s) 225, 493, 845, 1241
BslI CCNNNNNNNGG 3 cut(s) 175, 408, 409
BsmAI GTCTC 8 cut(s) 235, 293, 380, 386, 395, 452, 1130, 1355
BsmI GAATGC 1 cut(s) 1067
BsnI GGCC 1 cut(s) 1287
Bso31I GGTCTC 2 cut(s) 235, 1355
BsoBI CYCGRG 1 cut(s) 659
Bsp119I TTCGAA 1 cut(s) 730
Bsp1286I GDGCHC 4 cut(s) 295, 328, 706, 810
Bsp1407I TGTACA 1 cut(s) 1267
Bsp143I GATC 1 cut(s) 208
BspACI CCGC 2 cut(s) 1010, 1048
BspANI GGCC 1 cut(s) 1287
BspCNI CTCAG 1 cut(s) 551
BspFNI CGCG 2 cut(s) 966, 1010
BspLI GGNNCC 1 cut(s) 916
BspMAI CTGCAG 1 cut(s) 75
BspT104I TTCGAA 1 cut(s) 730
BspTI CTTAAG 1 cut(s) 113
BspTNI GGTCTC 2 cut(s) 235, 1355
BsrDI GCAATG 1 cut(s) 780
BsrGI TGTACA 1 cut(s) 1267
BsrI ACTGG 4 cut(s) 265, 300, 644, 1351
BssECI CCNNGG 2 cut(s) 225, 1281
BssMI GATC 1 cut(s) 208
BssSI CACGAG 2 cut(s) 699, 705
BssT1I CCWWGG 1 cut(s) 1281
Bst2BI CACGAG 2 cut(s) 699, 705
Bst4CI ACNGT 3 cut(s) 982, 997, 1318
BstAFI CTTAAG 1 cut(s) 113
BstAPI GCANNNNNTGC 1 cut(s) 70
BstAUI TGTACA 1 cut(s) 1267
BstBI TTCGAA 1 cut(s) 730
BstC8I GCNNGC 3 cut(s) 925, 1257, 1335
BstDEI CTNAG 1 cut(s) 538
BstENI CCTNNNNNAGG 1 cut(s) 173
BstF5I GGATG 6 cut(s) 366, 450, 489, 1147, 1190, 1398
BstFNI CGCG 2 cut(s) 966, 1010
BstH2I RGCGCY 1 cut(s) 140
BstHHI GCGC 2 cut(s) 139, 968
BstKTI GATC 1 cut(s) 211
BstMAI GTCTC 8 cut(s) 235, 293, 380, 386, 395, 452, 1130, 1355
BstMBI GATC 1 cut(s) 208
BstMWI GCNNNNNNNGC 7 cut(s) 70, 323, 620, 776, 814, 823, 914
BstNSI RCATGY 3 cut(s) 206, 563, 690
BstSCI CCNGG 1 cut(s) 224
BstSFI CTRYAG 2 cut(s) 71, 1413
BstSLI GKGCMC 2 cut(s) 295, 328
BstUI CGCG 2 cut(s) 966, 1010
BstV1I GCAGC 5 cut(s) 57, 146, 626, 757, 955
BstV2I GAAGAC 3 cut(s) 263, 1106, 1365
BstXI CCANNNNNNTGG 1 cut(s) 1138
BsuRI GGCC 1 cut(s) 1287
BtgZI GCGATG 1 cut(s) 220
BtsCI GGATG 6 cut(s) 366, 450, 489, 1147, 1190, 1398
BtsIMutI CAGTG 2 cut(s) 293, 861
Cac8I GCNNGC 3 cut(s) 925, 1257, 1335
CfoI GCGC 2 cut(s) 139, 968
Cfr13I GGNCC 2 cut(s) 795, 1285
CseI GACGC 1 cut(s) 514
Csp6I GTAC 5 cut(s) 166, 563, 656, 1268, 1409
CviAII CATG 7 cut(s) 17, 130, 154, 203, 560, 670, 687
CviQI GTAC 5 cut(s) 166, 563, 656, 1268, 1409
DdeI CTNAG 1 cut(s) 538
DpnI GATC 1 cut(s) 210
DpnII GATC 1 cut(s) 208
Eco130I CCWWGG 1 cut(s) 1281
Eco31I GGTCTC 2 cut(s) 235, 1355
Eco47I GGWCC 1 cut(s) 795
Eco57I CTGAAG 1 cut(s) 250
Eco88I CYCGRG 1 cut(s) 659
EcoNI CCTNNNNNAGG 1 cut(s) 173
EcoO109I RGGNCCY 1 cut(s) 1285
EcoRI GAATTC 2 cut(s) 348, 743
EcoT14I CCWWGG 1 cut(s) 1281
EcoT22I ATGCAT 1 cut(s) 1069
ErhI CCWWGG 1 cut(s) 1281
FaeI CATG 7 cut(s) 20, 133, 157, 206, 563, 673, 690
FalI AAGNNNNNCTT 2 cut(s) 1143, 1175
FatI CATG 7 cut(s) 16, 129, 153, 202, 559, 669, 686
FauNDI CATATG 2 cut(s) 319, 904
FblI GTMKAC 1 cut(s) 1313
Fnu4HI GCNGC 5 cut(s) 71, 135, 615, 771, 969
FokI GGATG 6 cut(s) 353, 437, 476, 1154, 1197, 1405
Fsp4HI GCNGC 5 cut(s) 71, 135, 615, 771, 969
FspBI CTAG 4 cut(s) 252, 284, 930, 1173
GlaI GCGC 2 cut(s) 138, 967
GluI GCNGC 5 cut(s) 71, 135, 615, 771, 969
GsaI CCCAGC 1 cut(s) 957
GsuI CTGGAG 1 cut(s) 317
HaeII RGCGCY 1 cut(s) 140
HaeIII GGCC 1 cut(s) 1287
HapII CCGG 4 cut(s) 225, 493, 845, 1241
HgaI GACGC 1 cut(s) 514
HhaI GCGC 2 cut(s) 139, 968
Hin1II CATG 7 cut(s) 20, 133, 157, 206, 563, 673, 690
Hin6I GCGC 2 cut(s) 137, 966
HinP1I GCGC 2 cut(s) 137, 966
HindIII AAGCTT 1 cut(s) 341
HinfI GANTC 5 cut(s) 301, 517, 697, 958, 1421
HpaII CCGG 4 cut(s) 225, 493, 845, 1241
HphI GGTGA 3 cut(s) 133, 169, 574
Hpy166II GTNNAC 4 cut(s) 293, 311, 1270, 1314
Hpy188I TCNGA 3 cut(s) 525, 840, 1061
Hpy188III TCNNGA 3 cut(s) 103, 502, 785
Hpy8I GTNNAC 4 cut(s) 293, 311, 1270, 1314
HpyAV CCTTC 3 cut(s) 77, 112, 640
HpyCH4III ACNGT 3 cut(s) 982, 997, 1318
HpyCH4IV ACGT 1 cut(s) 53
HpyF10VI GCNNNNNNNGC 7 cut(s) 70, 323, 620, 776, 814, 823, 914
HpyF3I CTNAG 1 cut(s) 538
HpySE526I ACGT 1 cut(s) 53
Hsp92II CATG 7 cut(s) 20, 133, 157, 206, 563, 673, 690
HspAI GCGC 2 cut(s) 137, 966
Kzo9I GATC 1 cut(s) 208
LmnI GCTCC 5 cut(s) 611, 914, 950, 1093, 1199
Lsp1109I GCAGC 5 cut(s) 57, 146, 626, 757, 955
LweI GCATC 3 cut(s) 150, 860, 1383
MaeI CTAG 4 cut(s) 252, 284, 930, 1173
MaeII ACGT 1 cut(s) 53
MaeIII GTNAC 3 cut(s) 256, 976, 1146
MalI GATC 1 cut(s) 210
MboI GATC 1 cut(s) 208
MboII GAAGA 7 cut(s) 39, 263, 470, 512, 739, 1106, 1365
MhlI GDGCHC 4 cut(s) 295, 328, 706, 810
MluCI AATT 5 cut(s) 348, 433, 743, 876, 1357
MlyI GAGTC 2 cut(s) 295, 691
MmeI TCCRAC 3 cut(s) 178, 589, 1408
Mph1103I ATGCAT 1 cut(s) 1069
MroXI GAANNNNTTC 1 cut(s) 726
MseI TTAA 5 cut(s) 114, 588, 645, 738, 1341
MslI CAYNNNNRTG 3 cut(s) 158, 1136, 1241
MspA1I CMGCKG 1 cut(s) 770
MspCI CTTAAG 1 cut(s) 113
MspI CCGG 4 cut(s) 225, 493, 845, 1241
MspR9I CCNGG 1 cut(s) 226
Mva1269I GAATGC 1 cut(s) 1067
MvnI CGCG 2 cut(s) 966, 1010
MwoI GCNNNNNNNGC 7 cut(s) 70, 323, 620, 776, 814, 823, 914
NciI CCSGG 1 cut(s) 226
NdeI CATATG 2 cut(s) 319, 904
NdeII GATC 1 cut(s) 208
NlaIII CATG 7 cut(s) 20, 133, 157, 206, 563, 673, 690
NlaIV GGNNCC 1 cut(s) 916
NmuCI GTSAC 1 cut(s) 976
NsiI ATGCAT 1 cut(s) 1069
NspI RCATGY 3 cut(s) 206, 563, 690
NspV TTCGAA 1 cut(s) 730
OliI CACNNNNGTG 1 cut(s) 1241
PciI ACATGT 1 cut(s) 202
PctI GAATGC 1 cut(s) 1067
PdmI GAANNNNTTC 1 cut(s) 726
PfeI GAWTC 3 cut(s) 517, 958, 1421
PflFI GACNNNGTC 1 cut(s) 1309
PkrI GCNGC 5 cut(s) 72, 136, 616, 772, 970
PleI GAGTC 2 cut(s) 295, 691
PpsI GAGTC 2 cut(s) 295, 691
PscI ACATGT 1 cut(s) 202
PspFI CCCAGC 1 cut(s) 953
PspN4I GGNNCC 1 cut(s) 916
PspPI GGNCC 2 cut(s) 795, 1285
PstI CTGCAG 1 cut(s) 75
PsyI GACNNNGTC 1 cut(s) 1309
PvuII CAGCTG 1 cut(s) 770
RsaI GTAC 5 cut(s) 167, 564, 657, 1269, 1410
RsaNI GTAC 5 cut(s) 166, 563, 656, 1268, 1409
RseI CAYNNNNRTG 3 cut(s) 158, 1136, 1241
SaqAI TTAA 5 cut(s) 114, 588, 645, 738, 1341
SatI GCNGC 5 cut(s) 71, 135, 615, 771, 969
Sau3AI GATC 1 cut(s) 208
Sau96I GGNCC 2 cut(s) 795, 1285
SchI GAGTC 2 cut(s) 295, 691
ScrFI CCNGG 1 cut(s) 226
SduI GDGCHC 4 cut(s) 295, 328, 706, 810
SfaNI GCATC 3 cut(s) 150, 860, 1383
SfcI CTRYAG 2 cut(s) 71, 1413
SfuI TTCGAA 1 cut(s) 730
SinI GGWCC 1 cut(s) 795
SmiMI CAYNNNNRTG 3 cut(s) 158, 1136, 1241
SmlI CTYRAG 2 cut(s) 113, 1273
SmoI CTYRAG 2 cut(s) 113, 1273
SpeI ACTAGT 1 cut(s) 251
Sse9I AATT 5 cut(s) 348, 433, 743, 876, 1357
SsiI CCGC 2 cut(s) 1010, 1048
SspMI CTAG 4 cut(s) 252, 284, 930, 1173
StyD4I CCNGG 1 cut(s) 224
StyI CCWWGG 1 cut(s) 1281
TaaI ACNGT 3 cut(s) 982, 997, 1318
TaiI ACGT 1 cut(s) 56
TaqI TCGA 2 cut(s) 207, 730
TasI AATT 5 cut(s) 348, 433, 743, 876, 1357
TatI WGTACW 2 cut(s) 655, 1267
TfiI GAWTC 3 cut(s) 517, 958, 1421
Tru1I TTAA 5 cut(s) 114, 588, 645, 738, 1341
Tru9I TTAA 5 cut(s) 114, 588, 645, 738, 1341
TscAI CASTG 2 cut(s) 300, 861
TseFI GTSAC 1 cut(s) 976
TseI GCWGC 5 cut(s) 70, 134, 614, 770, 968
Tsp45I GTSAC 1 cut(s) 976
TspDTI ATGAA 3 cut(s) 33, 341, 686
TspGWI ACGGA 2 cut(s) 110, 175
TspRI CASTG 2 cut(s) 300, 861
Tth111I GACNNNGTC 1 cut(s) 1309
Vha464I CTTAAG 1 cut(s) 113
VneI GTGCAC 1 cut(s) 291
VpaK11BI GGWCC 1 cut(s) 795
XagI CCTNNNNNAGG 1 cut(s) 173
XapI RAATTY 3 cut(s) 348, 433, 743
XceI RCATGY 3 cut(s) 206, 563, 690
XmiI GTMKAC 1 cut(s) 1313
XmnI GAANNNNTTC 1 cut(s) 726
XspI CTAG 4 cut(s) 252, 284, 930, 1173
Zsp2I ATGCAT 1 cut(s) 1069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.