pycom08g18500

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0024661
Physical Location & Seq
Forward (+)
18654587 .. 18655189
603 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 552 bp
ATGGATTTGTGGGTTCGTCGCCTAACGGGTTTTACTCTAGATGTGGGTTTGTTGAAGATGCAGCTTAATACTGGTTATGCTCAGAGTGGCGACCAACGTGCTTGTGAGGCTTTTGGGTTTGCTTGCCGTATGCAGAGGCAAGGATTGCATCCAAATCGGGTCACTCTTGCTAGCTTACTTCAGGCAGCGTCGCAGTTAGAAGCACTGGAAGAAGGACGTTCGGTCCATGGCTATGCTATTAGAAGAGGAATTGGTGGTTCGGATGAAGTGTTCGAAACCAGTCTCGTGGACATGTATATAACAAATGTGGATCCCCCTAGAAGGGTCGCCCGCATTTTTGGCGAAATGGATAGAAAGACTATTGGTTCTTGGAATGTGATGATTGCTGGTTATTTTAAAATAGAGCAACCCTTGGAGGCTTTCTGCCTTTTCTGTCAAGTGATGGAAGAAAATTTTGTCCCGGATTTGATAACTTTGTCCAATGGGATTCTGTCTTGTGCTTGCTTGAATTATTTGCGTCCAGGAAACAGTATCCACGGTTACATTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

184

Amino Acids

20.49

Weight (kDa)

5.92

Isoelectric Point (pI)

44.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 121 - 160 3.6e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024661)

Species Orthologous Gene IDs
pyrus_communis pycom08g18500 pycom08g18510 pycom111g04970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 331
AclWI GGATC 2 cut(s) 305, 318
AcsI RAATTY 1 cut(s) 451
AcuI CTGAAG 1 cut(s) 164
AfiI CCNNNNNNNGG 2 cut(s) 321, 322
AflIII ACRYGT 1 cut(s) 291
AgsI TTSAA 2 cut(s) 55, 508
AhdI GACNNNNNGTC 1 cut(s) 221
AjnI CCWGG 1 cut(s) 520
AloI GAACNNNNNNTCC 2 cut(s) 254, 286
AluBI AGCT 2 cut(s) 64, 174
AluI AGCT 2 cut(s) 64, 174
Alw26I GTCTC 1 cut(s) 287
AlwI GGATC 2 cut(s) 305, 318
ApeKI GCWGC 2 cut(s) 61, 185
ApoI RAATTY 1 cut(s) 451
AspS9I GGNCC 1 cut(s) 223
AsuC2I CCSGG 1 cut(s) 461
AsuII TTCGAA 1 cut(s) 273
AsuNHI GCTAGC 1 cut(s) 170
AvaII GGWCC 1 cut(s) 223
BamHI GGATCC 1 cut(s) 310
BauI CACGAG 1 cut(s) 284
BbvI GCAGC 2 cut(s) 73, 197
BccI CCATC 1 cut(s) 436
BceAI ACGGC 1 cut(s) 111
BcgI CGANNNNNNTGC 4 cut(s) 80, 114, 137, 171
BciT130I CCWGG 1 cut(s) 522
BciVI GTATCC 1 cut(s) 542
BcnI CCSGG 1 cut(s) 461
BcoDI GTCTC 1 cut(s) 287
BfaI CTAG 3 cut(s) 38, 171, 318
BfuI GTATCC 1 cut(s) 542
BisI GCNGC 2 cut(s) 62, 186
BlsI GCNGC 2 cut(s) 63, 187
Bme1390I CCNGG 2 cut(s) 461, 522
Bme18I GGWCC 1 cut(s) 223
BmeRI GACNNNNNGTC 1 cut(s) 221
BmgT120I GGNCC 1 cut(s) 223
BmiI GGNNCC 1 cut(s) 312
BmrFI CCNGG 2 cut(s) 461, 522
BmsI GCATC 2 cut(s) 48, 157
BmtI GCTAGC 1 cut(s) 174
Bpu14I TTCGAA 1 cut(s) 273
BpuMI CCSGG 1 cut(s) 461
BsaJI CCNNGG 3 cut(s) 226, 411, 535
Bsc4I CCNNNNNNNGG 2 cut(s) 321, 322
Bse1I ACTGG 3 cut(s) 76, 210, 279
BseBI CCWGG 1 cut(s) 522
BseDI CCNNGG 3 cut(s) 226, 411, 535
BseGI GGATG 2 cut(s) 148, 268
BseLI CCNNNNNNNGG 2 cut(s) 321, 322
BseMII CTCAG 1 cut(s) 95
BseNI ACTGG 3 cut(s) 76, 210, 279
BseXI GCAGC 2 cut(s) 73, 197
BsiSI CCGG 1 cut(s) 461
BslFI GGGAC 1 cut(s) 443
BslI CCNNNNNNNGG 2 cut(s) 321, 322
BsmAI GTCTC 1 cut(s) 287
BsmFI GGGAC 1 cut(s) 443
Bsp119I TTCGAA 1 cut(s) 273
Bsp143I GATC 1 cut(s) 310
Bsp19I CCATGG 1 cut(s) 226
BspACI CCGC 1 cut(s) 331
BspCNI CTCAG 1 cut(s) 94
BspLI GGNNCC 1 cut(s) 312
BspOI GCTAGC 1 cut(s) 174
BspPI GGATC 2 cut(s) 305, 318
BspT104I TTCGAA 1 cut(s) 273
BsrI ACTGG 3 cut(s) 76, 210, 279
BssECI CCNNGG 3 cut(s) 226, 411, 535
BssMI GATC 1 cut(s) 310
BssSI CACGAG 1 cut(s) 284
BssT1I CCWWGG 2 cut(s) 226, 411
Bst2BI CACGAG 1 cut(s) 284
Bst2UI CCWGG 1 cut(s) 522
Bst4CI ACNGT 2 cut(s) 530, 539
Bst6I CTCTTC 1 cut(s) 238
BstAPI GCANNNNNTGC 1 cut(s) 145
BstBI TTCGAA 1 cut(s) 273
BstC8I GCNNGC 4 cut(s) 124, 172, 331, 502
BstDEI CTNAG 1 cut(s) 81
BstDSI CCRYGG 2 cut(s) 226, 535
BstF5I GGATG 2 cut(s) 148, 268
BstKTI GATC 1 cut(s) 313
BstMAI GTCTC 1 cut(s) 287
BstMBI GATC 1 cut(s) 310
BstMWI GCNNNNNNNGC 3 cut(s) 107, 145, 339
BstNI CCWGG 1 cut(s) 522
BstNSI RCATGY 1 cut(s) 295
BstSCI CCNGG 2 cut(s) 459, 520
BstV1I GCAGC 2 cut(s) 73, 197
BstX2I RGATCY 1 cut(s) 310
BstXI CCANNNNNNTGG 1 cut(s) 286
BstYI RGATCY 1 cut(s) 310
BsuI GTATCC 1 cut(s) 542
BtgI CCRYGG 2 cut(s) 226, 535
BtsCI GGATG 2 cut(s) 148, 268
BtsIMutI CAGTG 1 cut(s) 203
Cac8I GCNNGC 4 cut(s) 124, 172, 331, 502
Cfr13I GGNCC 1 cut(s) 223
CseI GACGC 2 cut(s) 177, 506
CviAII CATG 2 cut(s) 227, 292
CviJI RGCY 5 cut(s) 64, 110, 174, 231, 419
CviKI_1 RGCY 5 cut(s) 64, 110, 174, 231, 419
DdeI CTNAG 1 cut(s) 81
DpnI GATC 1 cut(s) 312
DpnII GATC 1 cut(s) 310
DraI TTTAAA 1 cut(s) 397
DriI GACNNNNNGTC 1 cut(s) 221
Eam1104I CTCTTC 1 cut(s) 238
Eam1105I GACNNNNNGTC 1 cut(s) 221
EarI CTCTTC 1 cut(s) 238
Eco130I CCWWGG 2 cut(s) 226, 411
Eco47I GGWCC 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 164
EcoRII CCWGG 1 cut(s) 520
EcoT14I CCWWGG 2 cut(s) 226, 411
ErhI CCWWGG 2 cut(s) 226, 411
FaeI CATG 2 cut(s) 230, 295
FaiI YATR 7 cut(s) 78, 131, 228, 234, 293, 297, 299
FaqI GGGAC 1 cut(s) 443
FatI CATG 2 cut(s) 226, 291
FauI CCCGC 1 cut(s) 338
Fnu4HI GCNGC 2 cut(s) 62, 186
FokI GGATG 2 cut(s) 135, 275
Fsp4HI GCNGC 2 cut(s) 62, 186
FspBI CTAG 3 cut(s) 38, 171, 318
GluI GCNGC 2 cut(s) 62, 186
HapII CCGG 1 cut(s) 461
HgaI GACGC 2 cut(s) 177, 506
Hin1II CATG 2 cut(s) 230, 295
HinfI GANTC 1 cut(s) 487
HpaII CCGG 1 cut(s) 461
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 2 cut(s) 84, 262
Hpy188III TCNNGA 1 cut(s) 38
Hpy8I GTNNAC 1 cut(s) 289
Hpy99I CGWCG 2 cut(s) 21, 193
HpyAV CCTTC 2 cut(s) 206, 315
HpyCH4III ACNGT 2 cut(s) 530, 539
HpyCH4IV ACGT 2 cut(s) 97, 217
HpyCH4V TGCA 3 cut(s) 61, 133, 148
HpyF10VI GCNNNNNNNGC 3 cut(s) 107, 145, 339
HpyF3I CTNAG 1 cut(s) 81
HpySE526I ACGT 2 cut(s) 97, 217
Hsp92II CATG 2 cut(s) 230, 295
Kzo9I GATC 1 cut(s) 310
LpnPI CCDG 8 cut(s) 57, 167, 191, 292, 372, 474, 507, 534
Lsp1109I GCAGC 2 cut(s) 73, 197
LweI GCATC 2 cut(s) 48, 157
MaeI CTAG 3 cut(s) 38, 171, 318
MaeII ACGT 2 cut(s) 97, 217
MaeIII GTNAC 2 cut(s) 160, 539
MalI GATC 1 cut(s) 312
MboI GATC 1 cut(s) 310
MboII GAAGA 4 cut(s) 67, 221, 255, 458
MflI RGATCY 1 cut(s) 310
MluCI AATT 3 cut(s) 249, 451, 508
MnlI CCTC 4 cut(s) 100, 129, 239, 409
MseI TTAA 2 cut(s) 66, 396
MslI CAYNNNNRTG 1 cut(s) 231
MspI CCGG 1 cut(s) 461
MspR9I CCNGG 2 cut(s) 461, 522
MvaI CCWGG 1 cut(s) 522
MwoI GCNNNNNNNGC 3 cut(s) 107, 145, 339
NciI CCSGG 1 cut(s) 461
NcoI CCATGG 1 cut(s) 226
NdeII GATC 1 cut(s) 310
NheI GCTAGC 1 cut(s) 170
NlaIII CATG 2 cut(s) 230, 295
NlaIV GGNNCC 1 cut(s) 312
NmuCI GTSAC 1 cut(s) 160
NspI RCATGY 1 cut(s) 295
NspV TTCGAA 1 cut(s) 273
PciI ACATGT 1 cut(s) 291
PfeI GAWTC 1 cut(s) 487
PfoI TCCNGGA 2 cut(s) 459, 520
PkrI GCNGC 2 cut(s) 63, 187
PscI ACATGT 1 cut(s) 291
Psp6I CCWGG 1 cut(s) 520
PspGI CCWGG 1 cut(s) 520
PspN4I GGNNCC 1 cut(s) 312
PspPI GGNCC 1 cut(s) 223
PsuI RGATCY 1 cut(s) 310
RseI CAYNNNNRTG 1 cut(s) 231
SaqAI TTAA 2 cut(s) 66, 396
SatI GCNGC 2 cut(s) 62, 186
Sau3AI GATC 1 cut(s) 310
Sau96I GGNCC 1 cut(s) 223
ScrFI CCNGG 2 cut(s) 461, 522
SetI ASST 4 cut(s) 66, 100, 176, 220
SfaNI GCATC 2 cut(s) 48, 157
SfuI TTCGAA 1 cut(s) 273
SinI GGWCC 1 cut(s) 223
SmiMI CAYNNNNRTG 1 cut(s) 231
Sse9I AATT 3 cut(s) 249, 451, 508
SsiI CCGC 1 cut(s) 331
SspMI CTAG 3 cut(s) 38, 171, 318
StyD4I CCNGG 2 cut(s) 459, 520
StyI CCWWGG 2 cut(s) 226, 411
TaaI ACNGT 2 cut(s) 530, 539
TaiI ACGT 2 cut(s) 100, 220
TaqI TCGA 1 cut(s) 273
TaqII GACCGA 1 cut(s) 211
TasI AATT 3 cut(s) 249, 451, 508
TfiI GAWTC 1 cut(s) 487
Tru1I TTAA 2 cut(s) 66, 396
Tru9I TTAA 2 cut(s) 66, 396
TscAI CASTG 1 cut(s) 210
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 2 cut(s) 61, 185
Tsp45I GTSAC 1 cut(s) 160
TspDTI ATGAA 1 cut(s) 279
TspRI CASTG 1 cut(s) 210
VpaK11BI GGWCC 1 cut(s) 223
XapI RAATTY 1 cut(s) 451
XbaI TCTAGA 1 cut(s) 37
XceI RCATGY 1 cut(s) 295
XspI CTAG 3 cut(s) 38, 171, 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.