pycom09g06340

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0028436
Physical Location & Seq
Forward (+)
4666643 .. 4668506
1864 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 318 bp
ATGCCTGTGGAGCAGCTTGTTCAAACTCTTTGTGATACCAAGCAGGGGTACACGCAGTTTGGTGGTCTCCGGCCATTTGGTCTTTACATGAGTGATCCGAGCGGAAACTATGCTGGCTGGAAAGCTGCTGTGATTGGGGCTAACAACCAAGCTGCGCAGTCAATGCTTAAGCAGGATTACAAGGATGATATGACTAGGGAAGAGGGAGTCGAGCTAGCGCTGAAGGTGCTCAGTAAAACCATGGACAGCACAAGTCTCACCCCAGAGAAGCTTGAATTGGCTGAGGTCTTCGTATATATAACTAGCCGTGGTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019773 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome Proteasome_A_N

Protein Analysis

106

Amino Acids

11.56

Weight (kDa)

4.82

Isoelectric Point (pI)

47.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 28 - 95 4.8e-17 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028436)

Species Orthologous Gene IDs
malus_domestica MD09G1144000.v1.1
pyrus_communis pycom09g06340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 156
AccBSI CCGCTC 1 cut(s) 102
AciI CCGC 1 cut(s) 102
AclWI GGATC 1 cut(s) 89
AcoI YGGCCR 1 cut(s) 71
AcuI CTGAAG 1 cut(s) 242
AfaI GTAC 1 cut(s) 50
AfeI AGCGCT 1 cut(s) 219
AfiI CCNNNNNNNGG 1 cut(s) 45
AflII CTTAAG 1 cut(s) 167
AgsI TTSAA 2 cut(s) 23, 275
AjuI GAANNNNNNNTTGG 2 cut(s) 260, 292
AluBI AGCT 6 cut(s) 16, 125, 152, 214, 271, 315
AluI AGCT 6 cut(s) 16, 125, 152, 214, 271, 315
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 2 cut(s) 71, 260
AlwI GGATC 1 cut(s) 89
Aor51HI AGCGCT 1 cut(s) 219
AoxI GGCC 1 cut(s) 71
ApeKI GCWGC 3 cut(s) 13, 125, 152
AspLEI GCGC 2 cut(s) 157, 220
AsuHPI GGTGA 1 cut(s) 250
AsuNHI GCTAGC 1 cut(s) 214
BbsI GAAGAC 1 cut(s) 280
Bbv12I GWGCWC 1 cut(s) 231
BbvCI CCTCAGC 1 cut(s) 282
BbvI GCAGC 3 cut(s) 25, 112, 139
BceAI ACGGC 1 cut(s) 291
BcoDI GTCTC 2 cut(s) 71, 260
BfaI CTAG 4 cut(s) 195, 215, 303, 316
BfoI RGCGCY 1 cut(s) 221
BfrI CTTAAG 1 cut(s) 167
BisI GCNGC 3 cut(s) 14, 126, 153
BlsI GCNGC 3 cut(s) 15, 127, 154
BmtI GCTAGC 1 cut(s) 218
BpiI GAAGAC 1 cut(s) 280
Bpu10I CCTNAGC 1 cut(s) 282
BsaI GGTCTC 1 cut(s) 71
BsaJI CCNNGG 2 cut(s) 240, 307
Bsc4I CCNNNNNNNGG 1 cut(s) 45
BseDI CCNNGG 2 cut(s) 240, 307
BseGI GGATG 1 cut(s) 190
BseLI CCNNNNNNNGG 1 cut(s) 45
BseMII CTCAG 2 cut(s) 244, 273
BseXI GCAGC 3 cut(s) 25, 112, 139
BshFI GGCC 1 cut(s) 73
BsiHKAI GWGCWC 1 cut(s) 231
BsiSI CCGG 1 cut(s) 70
BslI CCNNNNNNNGG 1 cut(s) 45
BsmAI GTCTC 2 cut(s) 71, 260
BsnI GGCC 1 cut(s) 73
Bso31I GGTCTC 1 cut(s) 71
Bsp1286I GDGCHC 1 cut(s) 231
Bsp143I GATC 1 cut(s) 94
Bsp19I CCATGG 1 cut(s) 240
BspACI CCGC 1 cut(s) 102
BspANI GGCC 1 cut(s) 73
BspCNI CTCAG 2 cut(s) 243, 274
BspOI GCTAGC 1 cut(s) 218
BspPI GGATC 1 cut(s) 89
BspTI CTTAAG 1 cut(s) 167
BspTNI GGTCTC 1 cut(s) 71
BsrBI CCGCTC 1 cut(s) 102
BssECI CCNNGG 2 cut(s) 240, 307
BssMI GATC 1 cut(s) 94
BssT1I CCWWGG 1 cut(s) 240
Bst6I CTCTTC 1 cut(s) 195
BstAFI CTTAAG 1 cut(s) 167
BstAPI GCANNNNNTGC 1 cut(s) 163
BstC8I GCNNGC 2 cut(s) 115, 216
BstDEI CTNAG 2 cut(s) 230, 282
BstDSI CCRYGG 2 cut(s) 240, 307
BstF5I GGATG 1 cut(s) 190
BstH2I RGCGCY 1 cut(s) 221
BstHHI GCGC 2 cut(s) 157, 220
BstKTI GATC 1 cut(s) 97
BstMAI GTCTC 2 cut(s) 71, 260
BstMBI GATC 1 cut(s) 94
BstMWI GCNNNNNNNGC 4 cut(s) 10, 163, 226, 312
BstV1I GCAGC 3 cut(s) 25, 112, 139
BstV2I GAAGAC 1 cut(s) 280
BsuRI GGCC 1 cut(s) 73
BtgI CCRYGG 2 cut(s) 240, 307
BtsCI GGATG 1 cut(s) 190
Cac8I GCNNGC 2 cut(s) 115, 216
CfoI GCGC 2 cut(s) 157, 220
Csp6I GTAC 1 cut(s) 49
CviAII CATG 2 cut(s) 88, 241
CviQI GTAC 1 cut(s) 49
DdeI CTNAG 2 cut(s) 230, 282
DpnI GATC 1 cut(s) 96
DpnII GATC 1 cut(s) 94
EaeI YGGCCR 1 cut(s) 71
Eam1104I CTCTTC 1 cut(s) 195
EarI CTCTTC 1 cut(s) 195
Eco130I CCWWGG 1 cut(s) 240
Eco31I GGTCTC 1 cut(s) 71
Eco47III AGCGCT 1 cut(s) 219
Eco57I CTGAAG 1 cut(s) 242
EcoT14I CCWWGG 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 240
FaeI CATG 2 cut(s) 91, 244
FaiI YATR 7 cut(s) 89, 111, 191, 242, 295, 297, 299
FatI CATG 2 cut(s) 87, 240
Fnu4HI GCNGC 3 cut(s) 14, 126, 153
FokI GGATG 1 cut(s) 197
Fsp4HI GCNGC 3 cut(s) 14, 126, 153
FspBI CTAG 4 cut(s) 195, 215, 303, 316
FspI TGCGCA 1 cut(s) 156
GlaI GCGC 2 cut(s) 156, 219
GluI GCNGC 3 cut(s) 14, 126, 153
HaeII RGCGCY 1 cut(s) 221
HaeIII GGCC 1 cut(s) 73
HapII CCGG 1 cut(s) 70
HhaI GCGC 2 cut(s) 157, 220
Hin1II CATG 2 cut(s) 91, 244
Hin6I GCGC 2 cut(s) 155, 218
HinP1I GCGC 2 cut(s) 155, 218
HindIII AAGCTT 1 cut(s) 269
HinfI GANTC 1 cut(s) 207
HpaII CCGG 1 cut(s) 70
HphI GGTGA 1 cut(s) 250
Hpy166II GTNNAC 1 cut(s) 51
Hpy188I TCNGA 1 cut(s) 99
Hpy8I GTNNAC 1 cut(s) 51
HpyAV CCTTC 1 cut(s) 217
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 163, 226, 312
HpyF3I CTNAG 2 cut(s) 230, 282
Hsp92II CATG 2 cut(s) 91, 244
HspAI GCGC 2 cut(s) 155, 218
Kzo9I GATC 1 cut(s) 94
LmnI GCTCC 1 cut(s) 10
LpnPI CCDG 7 cut(s) 18, 29, 83, 99, 103, 158, 276
Lsp1109I GCAGC 3 cut(s) 25, 112, 139
MaeI CTAG 4 cut(s) 195, 215, 303, 316
MalI GATC 1 cut(s) 96
MbiI CCGCTC 1 cut(s) 102
MboI GATC 1 cut(s) 94
MboII GAAGA 2 cut(s) 212, 280
MhlI GDGCHC 1 cut(s) 231
MluCI AATT 1 cut(s) 275
MlyI GAGTC 1 cut(s) 216
MnlI CCTC 2 cut(s) 196, 277
MseI TTAA 1 cut(s) 168
MspCI CTTAAG 1 cut(s) 167
MspI CCGG 1 cut(s) 70
MwoI GCNNNNNNNGC 4 cut(s) 10, 163, 226, 312
NcoI CCATGG 1 cut(s) 240
NdeII GATC 1 cut(s) 94
NheI GCTAGC 1 cut(s) 214
NlaIII CATG 2 cut(s) 91, 244
NsbI TGCGCA 1 cut(s) 156
PkrI GCNGC 3 cut(s) 15, 127, 154
PleI GAGTC 1 cut(s) 215
PpsI GAGTC 1 cut(s) 215
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
SaqAI TTAA 1 cut(s) 168
SatI GCNGC 3 cut(s) 14, 126, 153
Sau3AI GATC 1 cut(s) 94
SchI GAGTC 1 cut(s) 216
SduI GDGCHC 1 cut(s) 231
SetI ASST 8 cut(s) 18, 127, 154, 216, 228, 273, 288, 317
SmlI CTYRAG 1 cut(s) 167
SmoI CTYRAG 1 cut(s) 167
Sse9I AATT 1 cut(s) 275
SsiI CCGC 1 cut(s) 102
SspMI CTAG 4 cut(s) 195, 215, 303, 316
StyI CCWWGG 1 cut(s) 240
TaqI TCGA 1 cut(s) 210
TasI AATT 1 cut(s) 275
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TseI GCWGC 3 cut(s) 13, 125, 152
Vha464I CTTAAG 1 cut(s) 167
XspI CTAG 4 cut(s) 195, 215, 303, 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.