pycom09g10900

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0029362
Physical Location & Seq
Forward (+)
9019322 .. 9021256
1935 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 615 bp
ATGAAGTATAAGAAGAAGAATCATACCTCAGCGTCAAGCTCCAAATTGTCAACAATGGTGAGGAGGAGAAGCACGACACTCACCTGGTTTTTCCTCATCTTTCTCTCTCTAATAGCCTTTGGAATCTTTGTCCCTGTCTTTGCTCTTCTACCTCCTCTCTCTTCCCACTCTCCCCACCAACAACAACTGCAGCACCATCCCAGCACCAAAAGGAATCAGGATCATGCCAAAAAGTTTGAAATTTCAGAGGACAAGTTCTGGAAGGATGGTCAGCCTTTCCAGATCATTGGGGGTGACTTGCACTATTTCCGGGTTCTTCCTGAGTACTGGGAAGATAGGTTGTTGAGAGCAAAGGCATTGGGATTAAATACGATTCAAACTTATGTTCCCTGGAACCTACATGAACCAAGAGCTGGTATTTTGGATTTTGACGGTATTGCAGATTTGGTTGCATTTCTCAAACTCTCCCAGAAGCTGGGTATTCTTGTAATGCTTCGACCTGGACCATATATATGTGCAGAGTGGGATTTGGGAGGTTTCCCTCCTTGGTTACTTGCCATCAATCCAGCTCCCAGACTACGATCGATCAGATCCCATTTTCCTTCAGTTGGTTGA

Protein Analysis

205

Amino Acids

23.43

Weight (kDa)

9.9

Isoelectric Point (pI)

54.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 85 - 200 8.7e-49 Glycosyl hydrolases family 35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0029362)

Species Orthologous Gene IDs
pyrus_communis pycom09g10900
rosa_multiflora Rmu_co8295193.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 413, 475
AclWI GGATC 2 cut(s) 228, 585
AcsI RAATTY 1 cut(s) 240
AcuI CTGAAG 1 cut(s) 588
AfaI GTAC 1 cut(s) 326
AfiI CCNNNNNNNGG 3 cut(s) 413, 475, 608
AgsI TTSAA 2 cut(s) 239, 377
AjnI CCWGG 3 cut(s) 83, 389, 499
AluBI AGCT 4 cut(s) 39, 413, 475, 569
AluI AGCT 4 cut(s) 39, 413, 475, 569
AlwI GGATC 2 cut(s) 228, 585
AlwNI CAGNNNCTG 1 cut(s) 475
ApeKI GCWGC 1 cut(s) 190
ApoI RAATTY 1 cut(s) 240
AspS9I GGNCC 1 cut(s) 503
AsuC2I CCSGG 1 cut(s) 311
AsuHPI GGTGA 3 cut(s) 70, 73, 305
AvaII GGWCC 1 cut(s) 503
BbvCI CCTCAGC 1 cut(s) 28
BbvI GCAGC 1 cut(s) 202
BccI CCATC 3 cut(s) 204, 260, 566
BciT130I CCWGG 3 cut(s) 85, 391, 501
BcnI CCSGG 1 cut(s) 311
BfmI CTRYAG 1 cut(s) 188
BisI GCNGC 1 cut(s) 191
BlsI GCNGC 1 cut(s) 192
BmcAI AGTACT 1 cut(s) 326
Bme1390I CCNGG 4 cut(s) 85, 311, 391, 501
Bme18I GGWCC 1 cut(s) 503
BmgT120I GGNCC 1 cut(s) 503
BmiI GGNNCC 1 cut(s) 395
BmrFI CCNGG 4 cut(s) 85, 311, 391, 501
BmrI ACTGGG 1 cut(s) 337
BmuI ACTGGG 1 cut(s) 337
Bpu10I CCTNAGC 1 cut(s) 28
BpuMI CCSGG 1 cut(s) 311
Bsa29I ATCGAT 1 cut(s) 584
BsaJI CCNNGG 2 cut(s) 389, 545
Bsc4I CCNNNNNNNGG 3 cut(s) 413, 475, 608
Bse1I ACTGG 1 cut(s) 332
BseBI CCWGG 3 cut(s) 85, 391, 501
BseCI ATCGAT 1 cut(s) 584
BseDI CCNNGG 2 cut(s) 389, 545
BseGI GGATG 2 cut(s) 196, 271
BseLI CCNNNNNNNGG 3 cut(s) 413, 475, 608
BseMII CTCAG 2 cut(s) 42, 312
BseNI ACTGG 1 cut(s) 332
BseRI GAGGAG 3 cut(s) 76, 79, 144
BseXI GCAGC 1 cut(s) 202
BseYI CCCAGC 2 cut(s) 200, 475
BsgI GTGCAG 1 cut(s) 537
Bsh1285I CGRYCG 1 cut(s) 584
BshVI ATCGAT 1 cut(s) 584
BsiEI CGRYCG 1 cut(s) 584
BsiSI CCGG 1 cut(s) 310
BslFI GGGAC 1 cut(s) 116
BslI CCNNNNNNNGG 3 cut(s) 413, 475, 608
BsmFI GGGAC 1 cut(s) 116
Bsp143I GATC 5 cut(s) 220, 282, 581, 585, 590
BspCNI CTCAG 2 cut(s) 41, 313
BspDI ATCGAT 1 cut(s) 584
BspLI GGNNCC 1 cut(s) 395
BspMAI CTGCAG 1 cut(s) 192
BspPI GGATC 2 cut(s) 228, 585
BspQI GCTCTTC 1 cut(s) 150
BsrI ACTGG 1 cut(s) 332
BssECI CCNNGG 2 cut(s) 389, 545
BssMI GATC 5 cut(s) 220, 282, 581, 585, 590
BssT1I CCWWGG 1 cut(s) 545
Bst2UI CCWGG 3 cut(s) 85, 391, 501
Bst4CI ACNGT 1 cut(s) 434
Bst6I CTCTTC 2 cut(s) 150, 166
BstDEI CTNAG 2 cut(s) 28, 321
BstF5I GGATG 2 cut(s) 196, 271
BstKTI GATC 5 cut(s) 223, 285, 584, 588, 593
BstMBI GATC 5 cut(s) 220, 282, 581, 585, 590
BstMCI CGRYCG 1 cut(s) 584
BstNI CCWGG 3 cut(s) 85, 391, 501
BstSCI CCNGG 4 cut(s) 83, 309, 389, 499
BstSFI CTRYAG 1 cut(s) 188
BstV1I GCAGC 1 cut(s) 202
BstX2I RGATCY 1 cut(s) 590
BstXI CCANNNNNNTGG 1 cut(s) 287
BstYI RGATCY 1 cut(s) 590
Bsu15I ATCGAT 1 cut(s) 584
BsuTUI ATCGAT 1 cut(s) 584
BtsCI GGATG 2 cut(s) 196, 271
CaiI CAGNNNCTG 1 cut(s) 475
Cfr13I GGNCC 1 cut(s) 503
ClaI ATCGAT 1 cut(s) 584
CseI GACGC 1 cut(s) 21
CsiI ACCWGGT 1 cut(s) 83
Csp6I GTAC 1 cut(s) 325
CviAII CATG 2 cut(s) 224, 401
CviJI RGCY 6 cut(s) 39, 116, 274, 413, 475, 569
CviKI_1 RGCY 6 cut(s) 39, 116, 274, 413, 475, 569
CviQI GTAC 1 cut(s) 325
DdeI CTNAG 2 cut(s) 28, 321
DpnI GATC 5 cut(s) 222, 284, 583, 587, 592
DpnII GATC 5 cut(s) 220, 282, 581, 585, 590
Eam1104I CTCTTC 2 cut(s) 150, 166
EarI CTCTTC 2 cut(s) 150, 166
Eco130I CCWWGG 1 cut(s) 545
Eco47I GGWCC 1 cut(s) 503
Eco57I CTGAAG 1 cut(s) 588
EcoRII CCWGG 3 cut(s) 83, 389, 499
EcoT14I CCWWGG 1 cut(s) 545
ErhI CCWWGG 1 cut(s) 545
FaeI CATG 2 cut(s) 227, 404
FaiI YATR 9 cut(s) 9, 24, 225, 384, 402, 508, 510, 512, 514
FaqI GGGAC 1 cut(s) 116
FatI CATG 2 cut(s) 223, 400
Fnu4HI GCNGC 1 cut(s) 191
FokI GGATG 2 cut(s) 183, 278
Fsp4HI GCNGC 1 cut(s) 191
GluI GCNGC 1 cut(s) 191
GsaI CCCAGC 2 cut(s) 204, 479
HapII CCGG 1 cut(s) 310
HgaI GACGC 1 cut(s) 21
Hin1II CATG 2 cut(s) 227, 404
HincII GTYRAC 1 cut(s) 51
HindII GTYRAC 1 cut(s) 51
HinfI GANTC 4 cut(s) 19, 123, 214, 373
HpaII CCGG 1 cut(s) 310
HphI GGTGA 3 cut(s) 70, 73, 305
Hpy166II GTNNAC 1 cut(s) 51
Hpy188I TCNGA 2 cut(s) 247, 590
Hpy188III TCNNGA 4 cut(s) 218, 259, 280, 320
Hpy8I GTNNAC 1 cut(s) 51
HpyAV CCTTC 2 cut(s) 256, 612
HpyCH4III ACNGT 1 cut(s) 434
HpyCH4V TGCA 5 cut(s) 190, 301, 440, 452, 518
HpyF3I CTNAG 2 cut(s) 28, 321
Hsp92II CATG 2 cut(s) 227, 404
Kzo9I GATC 5 cut(s) 220, 282, 581, 585, 590
LguI GCTCTTC 1 cut(s) 150
LmnI GCTCC 2 cut(s) 44, 574
Lsp1109I GCAGC 1 cut(s) 202
MabI ACCWGGT 1 cut(s) 83
MaeIII GTNAC 2 cut(s) 293, 549
MalI GATC 5 cut(s) 222, 284, 583, 587, 592
MboI GATC 5 cut(s) 220, 282, 581, 585, 590
MboII GAAGA 6 cut(s) 25, 28, 137, 153, 308, 344
MflI RGATCY 1 cut(s) 590
MluCI AATT 2 cut(s) 44, 240
MnlI CCTC 9 cut(s) 37, 54, 57, 104, 162, 165, 241, 527, 552
MseI TTAA 1 cut(s) 365
MslI CAYNNNNRTG 1 cut(s) 511
MspI CCGG 1 cut(s) 310
MspR9I CCNGG 4 cut(s) 85, 311, 391, 501
MvaI CCWGG 3 cut(s) 85, 391, 501
NciI CCSGG 1 cut(s) 311
NdeII GATC 5 cut(s) 220, 282, 581, 585, 590
NlaIII CATG 2 cut(s) 227, 404
NlaIV GGNNCC 1 cut(s) 395
NmuCI GTSAC 1 cut(s) 293
PciSI GCTCTTC 1 cut(s) 150
PfeI GAWTC 4 cut(s) 19, 123, 214, 373
PflMI CCANNNNNTGG 2 cut(s) 413, 475
PkrI GCNGC 1 cut(s) 192
Ple19I CGATCG 1 cut(s) 584
Psp6I CCWGG 3 cut(s) 83, 389, 499
PspFI CCCAGC 2 cut(s) 200, 475
PspGI CCWGG 3 cut(s) 83, 389, 499
PspN4I GGNNCC 1 cut(s) 395
PspPI GGNCC 1 cut(s) 503
PstI CTGCAG 1 cut(s) 192
PstNI CAGNNNCTG 1 cut(s) 475
PsuI RGATCY 1 cut(s) 590
PvuI CGATCG 1 cut(s) 584
RsaI GTAC 1 cut(s) 326
RsaNI GTAC 1 cut(s) 325
RseI CAYNNNNRTG 1 cut(s) 511
SapI GCTCTTC 1 cut(s) 150
SaqAI TTAA 1 cut(s) 365
SatI GCNGC 1 cut(s) 191
Sau3AI GATC 5 cut(s) 220, 282, 581, 585, 590
Sau96I GGNCC 1 cut(s) 503
ScaI AGTACT 1 cut(s) 326
ScrFI CCNGG 4 cut(s) 85, 311, 391, 501
SexAI ACCWGGT 1 cut(s) 83
SfcI CTRYAG 1 cut(s) 188
SinI GGWCC 1 cut(s) 503
SmiMI CAYNNNNRTG 1 cut(s) 511
Sse9I AATT 2 cut(s) 44, 240
StyD4I CCNGG 4 cut(s) 83, 309, 389, 499
StyI CCWWGG 1 cut(s) 545
TaaI ACNGT 1 cut(s) 434
TaqI TCGA 2 cut(s) 496, 584
TasI AATT 2 cut(s) 44, 240
TatI WGTACW 1 cut(s) 324
TfiI GAWTC 4 cut(s) 19, 123, 214, 373
Tru1I TTAA 1 cut(s) 365
Tru9I TTAA 1 cut(s) 365
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 1 cut(s) 190
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 2 cut(s) 17, 417
Van91I CCANNNNNTGG 2 cut(s) 413, 475
VpaK11BI GGWCC 1 cut(s) 503
XapI RAATTY 1 cut(s) 240
ZrmI AGTACT 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.