pycom09g17460

RNA polymerase II transcription elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0021905
Physical Location & Seq
Forward (+)
17966452 .. 17969288
2837 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1155 bp
ATGGCGACGAATCGGAAGAGCAACGAAGACGACGAGCCAAACGGTGCTCCGGAGACCGACCGGTGGTACAGCCTAACCCTAGGTTCTTCCTTCGTGGACCACCGTCCCTCCCCCAAGTTCTGCACCTTACGCTATGAATTTAAACCGGCTTCAATTGACAAGAACCAACCAGGGTTGTTACACAAGAGCAAGGACAATAGAGTTGCATTAGAATTTCACAACAACCAACACGGAAAACCCAAAGTGACTTTTGAAGGTGTGAGTGAGGATCACAAGAAAGACGAGGCCGTTTTGTTCTTTGATGGTGAGGGTTTTCGGTTGGAGAGGTTGCACCGAGCAGTGAAGCGGTTGAGGCATGTCAGGCTGCCTGGTGAATCTGCAGCTCCAGTGGCCGCAGTCACGAGTACATCAGTTTCAGCGGTGGAATCTTATTCTCCACCAACCGGTGGCAAAAGACCAAAGCTTCAGTCGTTGAACAAGGACATTCTTCCTAATATGCCACCGGTGGAGTTGAAAGAAATTGATATCAACAGCTTAGCGAGCTTAGACCCAGGGCCAAAACCTGAAACCAGCGAGGATTTGGACTGTCCGCCTTCCTTACCAAATCCACCAACTGCATCTCCTGATGCACAAAGAAGCGATGAAATGGAGGAAGAAGTGGATGTTGTCATTGATGATGATGACAATGAAGATGACGGTGATAATGATGATGATATATCTGAGAAGGGAAGATCTCCTGGAAAGGAATTTCACACCGGCATTGACATTAACTTACCTCATCCAGGAGATATGGATGATGAGATTGCTGATGCGGATATAAGTGATGATGTGATGTATAATGGCCCAAATGCTGCCAAAGCGCTCAGAGGTCAGGTTAATGCTGAGGTGGGAGAGAAGCACACTGATATGGATGATGAGGTTGTGGATGTTAGTGATGATGTGATAGATAACGGCCCAAATGCTGCAGAAGCCCTCAAAGCTCAGGTGAATGCCGAGGTGGGAGAGAAAGAGACGTCAAGCTCAAATGATACTAGTGGGAGTGAGAGTAGCAGTGGAAGCGGAAGCGGAAGTGGAAGCGAAAGTGAAAGCGAGAGTGAGAGTAGCAGCAGTGATAATGAAAGCAGTGAAGGTGGCGACTCAGTAAATTCTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EAF

Protein Analysis

385

Amino Acids

41.3

Weight (kDa)

4.35

Isoelectric Point (pI)

48.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EAF PF09816 23 - 121 9.9e-22 RNA polymerase II transcription elongation factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021905)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38050
malus_domestica MD09G1260000.v1.1
prunus_persica Prupe.3G151600_v2.0.a1
pyrus_communis pycom09g17460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1016
AccB7I CCANNNNNTGG 1 cut(s) 446
AccIII TCCGGA 1 cut(s) 49
AciI CCGC 7 cut(s) 346, 393, 419, 590, 812, 1059, 1065
AclWI GGATC 1 cut(s) 276
AcoI YGGCCR 1 cut(s) 390
AcsI RAATTY 4 cut(s) 137, 212, 746, 1144
AcuI CTGAAG 1 cut(s) 449
AcyI GRCGYC 1 cut(s) 1013
AfaI GTAC 2 cut(s) 68, 406
AfeI AGCGCT 1 cut(s) 861
AfiI CCNNNNNNNGG 4 cut(s) 63, 443, 446, 782
AgeI ACCGGT 3 cut(s) 60, 443, 502
AgsI TTSAA 4 cut(s) 153, 254, 475, 514
AhlI ACTAGT 1 cut(s) 1031
AjnI CCWGG 5 cut(s) 169, 367, 550, 736, 781
AluBI AGCT 6 cut(s) 383, 463, 534, 543, 980, 1020
AluI AGCT 6 cut(s) 383, 463, 534, 543, 980, 1020
Alw21I GWGCWC 1 cut(s) 49
Alw26I GTCTC 2 cut(s) 47, 1004
AlwI GGATC 1 cut(s) 276
Aor13HI TCCGGA 1 cut(s) 49
Aor51HI AGCGCT 1 cut(s) 861
AoxI GGCC 5 cut(s) 285, 390, 554, 841, 952
ApeKI GCWGC 5 cut(s) 364, 380, 851, 962, 1104
ApoI RAATTY 4 cut(s) 137, 212, 746, 1144
ArsI GACNNNNNNTTYG 2 cut(s) 452, 484
AsiGI ACCGGT 3 cut(s) 60, 443, 502
AspA2I CCTAGG 1 cut(s) 79
AspLEI GCGC 1 cut(s) 862
AspS9I GGNCC 4 cut(s) 97, 554, 842, 953
AsuHPI GGTGA 4 cut(s) 317, 383, 710, 997
AvaII GGWCC 1 cut(s) 97
AvrII CCTAGG 1 cut(s) 79
BauI CACGAG 1 cut(s) 400
BbsI GAAGAC 1 cut(s) 33
Bbv12I GWGCWC 1 cut(s) 49
BbvCI CCTCAGC 1 cut(s) 882
BbvI GCAGC 5 cut(s) 351, 392, 838, 949, 1116
BccI CCATC 1 cut(s) 296
BceAI ACGGC 2 cut(s) 272, 967
BciT130I CCWGG 5 cut(s) 171, 369, 552, 738, 783
BcoDI GTCTC 2 cut(s) 47, 1004
BcuI ACTAGT 1 cut(s) 1031
BfaI CTAG 3 cut(s) 80, 1032, 1153
BfmI CTRYAG 2 cut(s) 378, 963
BfoI RGCGCY 1 cut(s) 863
BglII AGATCT 1 cut(s) 731
BisI GCNGC 6 cut(s) 365, 381, 393, 852, 963, 1105
BlnI CCTAGG 1 cut(s) 79
BlpI GCTNAGC 1 cut(s) 535
BlsI GCNGC 6 cut(s) 366, 382, 394, 853, 964, 1106
Bme1390I CCNGG 5 cut(s) 171, 369, 552, 738, 783
Bme18I GGWCC 1 cut(s) 97
BmgT120I GGNCC 4 cut(s) 97, 554, 842, 953
BmrFI CCNGG 5 cut(s) 171, 369, 552, 738, 783
BmsI GCATC 3 cut(s) 616, 626, 799
BoxI GACNNNNGTC 1 cut(s) 102
BpiI GAAGAC 1 cut(s) 33
BpmI CTGGAG 1 cut(s) 369
Bpu10I CCTNAGC 2 cut(s) 882, 981
Bpu1102I GCTNAGC 1 cut(s) 535
BsaHI GRCGYC 1 cut(s) 1013
BsaI GGTCTC 1 cut(s) 47
BsaJI CCNNGG 5 cut(s) 79, 170, 550, 551, 993
BsaWI WCCGGW 4 cut(s) 49, 60, 443, 502
BsaXI ACNNNNNCTCC 6 cut(s) 92, 122, 604, 634, 1030, 1060
Bsc4I CCNNNNNNNGG 4 cut(s) 63, 443, 446, 782
Bse118I RCCGGY 5 cut(s) 60, 145, 443, 502, 755
Bse1I ACTGG 1 cut(s) 386
BseAI TCCGGA 1 cut(s) 49
BseBI CCWGG 5 cut(s) 171, 369, 552, 738, 783
BseDI CCNNGG 5 cut(s) 79, 170, 550, 551, 993
BseGI GGATG 5 cut(s) 667, 778, 799, 916, 931
BseLI CCNNNNNNNGG 4 cut(s) 63, 443, 446, 782
BseMII CTCAG 5 cut(s) 711, 873, 877, 995, 1152
BseNI ACTGG 1 cut(s) 386
BseXI GCAGC 5 cut(s) 351, 392, 838, 949, 1116
BsgI GTGCAG 1 cut(s) 106
Bsh1285I CGRYCG 1 cut(s) 61
BshFI GGCC 5 cut(s) 287, 392, 556, 843, 954
BshTI ACCGGT 3 cut(s) 60, 443, 502
BsiEI CGRYCG 1 cut(s) 61
BsiHKAI GWGCWC 1 cut(s) 49
BsiSI CCGG 6 cut(s) 50, 61, 146, 444, 503, 756
BslFI GGGAC 1 cut(s) 90
BslI CCNNNNNNNGG 4 cut(s) 63, 443, 446, 782
BsmAI GTCTC 2 cut(s) 47, 1004
BsmBI CGTCTC 1 cut(s) 1004
BsmFI GGGAC 1 cut(s) 90
BsmI GAATGC 1 cut(s) 994
BsnI GGCC 5 cut(s) 287, 392, 556, 843, 954
Bso31I GGTCTC 1 cut(s) 47
Bsp1286I GDGCHC 1 cut(s) 49
Bsp13I TCCGGA 1 cut(s) 49
Bsp143I GATC 2 cut(s) 268, 731
Bsp1720I GCTNAGC 1 cut(s) 535
BspACI CCGC 7 cut(s) 346, 393, 419, 590, 812, 1059, 1065
BspANI GGCC 5 cut(s) 287, 392, 556, 843, 954
BspCNI CTCAG 5 cut(s) 712, 874, 876, 994, 1151
BspEI TCCGGA 1 cut(s) 49
BspMAI CTGCAG 2 cut(s) 382, 967
BspPI GGATC 1 cut(s) 276
BspQI GCTCTTC 1 cut(s) 11
BspTNI GGTCTC 1 cut(s) 47
BsrFI RCCGGY 5 cut(s) 60, 145, 443, 502, 755
BsrI ACTGG 1 cut(s) 386
BssAI RCCGGY 5 cut(s) 60, 145, 443, 502, 755
BssECI CCNNGG 5 cut(s) 79, 170, 550, 551, 993
BssMI GATC 2 cut(s) 268, 731
BssNI GRCGYC 1 cut(s) 1013
BssSI CACGAG 1 cut(s) 400
BssT1I CCWWGG 1 cut(s) 79
Bst2BI CACGAG 1 cut(s) 400
Bst2UI CCWGG 5 cut(s) 171, 369, 552, 738, 783
Bst4CI ACNGT 4 cut(s) 44, 104, 587, 698
Bst6I CTCTTC 1 cut(s) 11
BstACI GRCGYC 1 cut(s) 1013
BstC8I GCNNGC 1 cut(s) 541
BstDEI CTNAG 7 cut(s) 535, 544, 720, 863, 882, 981, 1138
BstENI CCTNNNNNAGG 1 cut(s) 780
BstF5I GGATG 5 cut(s) 667, 778, 799, 916, 931
BstH2I RGCGCY 1 cut(s) 863
BstHHI GCGC 1 cut(s) 862
BstKTI GATC 2 cut(s) 271, 734
BstMAI GTCTC 2 cut(s) 47, 1004
BstMBI GATC 2 cut(s) 268, 731
BstMCI CGRYCG 1 cut(s) 61
BstMWI GCNNNNNNNGC 9 cut(s) 129, 352, 361, 389, 540, 857, 968, 977, 1056
BstNI CCWGG 5 cut(s) 171, 369, 552, 738, 783
BstNSI RCATGY 1 cut(s) 359
BstPAI GACNNNNGTC 1 cut(s) 102
BstSCI CCNGG 5 cut(s) 169, 367, 550, 736, 781
BstSFI CTRYAG 2 cut(s) 378, 963
BstV1I GCAGC 5 cut(s) 351, 392, 838, 949, 1116
BstV2I GAAGAC 1 cut(s) 33
BstX2I RGATCY 1 cut(s) 731
BstYI RGATCY 1 cut(s) 731
BsuRI GGCC 5 cut(s) 287, 392, 556, 843, 954
BtgZI GCGATG 1 cut(s) 654
BtsCI GGATG 5 cut(s) 667, 778, 799, 916, 931
BtsI GCAGTG 4 cut(s) 345, 1057, 1114, 1129
BtsIMutI CAGTG 6 cut(s) 345, 393, 900, 1057, 1114, 1129
Cac8I GCNNGC 1 cut(s) 541
CfoI GCGC 1 cut(s) 862
Cfr10I RCCGGY 5 cut(s) 60, 145, 443, 502, 755
Cfr13I GGNCC 4 cut(s) 97, 554, 842, 953
Csp6I GTAC 2 cut(s) 67, 405
CspAI ACCGGT 3 cut(s) 60, 443, 502
CviAII CATG 1 cut(s) 356
CviQI GTAC 2 cut(s) 67, 405
DdeI CTNAG 7 cut(s) 535, 544, 720, 863, 882, 981, 1138
DpnI GATC 2 cut(s) 270, 733
DpnII GATC 2 cut(s) 268, 731
DraI TTTAAA 1 cut(s) 142
EaeI YGGCCR 1 cut(s) 390
Eam1104I CTCTTC 1 cut(s) 11
EarI CTCTTC 1 cut(s) 11
EciI GGCGGA 1 cut(s) 579
Eco130I CCWWGG 1 cut(s) 79
Eco31I GGTCTC 1 cut(s) 47
Eco32I GATATC 1 cut(s) 526
Eco47I GGWCC 1 cut(s) 97
Eco47III AGCGCT 1 cut(s) 861
Eco57I CTGAAG 1 cut(s) 449
EcoNI CCTNNNNNAGG 1 cut(s) 780
EcoRII CCWGG 5 cut(s) 169, 367, 550, 736, 781
EcoRV GATATC 1 cut(s) 526
EcoT14I CCWWGG 1 cut(s) 79
ErhI CCWWGG 1 cut(s) 79
Esp3I CGTCTC 1 cut(s) 1004
FaeI CATG 1 cut(s) 359
FaiI YATR 8 cut(s) 135, 357, 497, 716, 791, 818, 837, 908
FaqI GGGAC 1 cut(s) 90
FatI CATG 1 cut(s) 355
Fnu4HI GCNGC 6 cut(s) 365, 381, 393, 852, 963, 1105
FokI GGATG 5 cut(s) 674, 765, 806, 923, 938
Fsp4HI GCNGC 6 cut(s) 365, 381, 393, 852, 963, 1105
FspBI CTAG 3 cut(s) 80, 1032, 1153
GlaI GCGC 1 cut(s) 861
GluI GCNGC 6 cut(s) 365, 381, 393, 852, 963, 1105
GsuI CTGGAG 1 cut(s) 369
HaeII RGCGCY 1 cut(s) 863
HaeIII GGCC 5 cut(s) 287, 392, 556, 843, 954
HapII CCGG 6 cut(s) 50, 61, 146, 444, 503, 756
HhaI GCGC 1 cut(s) 862
Hin1I GRCGYC 1 cut(s) 1013
Hin1II CATG 1 cut(s) 359
Hin6I GCGC 1 cut(s) 860
HinP1I GCGC 1 cut(s) 860
HindIII AAGCTT 1 cut(s) 461
HinfI GANTC 4 cut(s) 10, 374, 425, 1136
HpaII CCGG 6 cut(s) 50, 61, 146, 444, 503, 756
HphI GGTGA 4 cut(s) 317, 383, 710, 997
Hpy166II GTNNAC 1 cut(s) 97
Hpy188I TCNGA 3 cut(s) 15, 721, 866
Hpy188III TCNNGA 3 cut(s) 50, 400, 623
Hpy8I GTNNAC 1 cut(s) 97
Hpy99I CGWCG 2 cut(s) 10, 35
HpyAV CCTTC 5 cut(s) 100, 248, 603, 718, 1121
HpyCH4III ACNGT 4 cut(s) 44, 104, 587, 698
HpyCH4IV ACGT 1 cut(s) 1013
HpyCH4V TGCA 7 cut(s) 123, 206, 331, 380, 617, 629, 965
HpyF10VI GCNNNNNNNGC 9 cut(s) 129, 352, 361, 389, 540, 857, 968, 977, 1056
HpyF3I CTNAG 7 cut(s) 535, 544, 720, 863, 882, 981, 1138
HpySE526I ACGT 1 cut(s) 1013
Hsp92I GRCGYC 1 cut(s) 1013
Hsp92II CATG 1 cut(s) 359
HspAI GCGC 1 cut(s) 860
Kpn2I TCCGGA 1 cut(s) 49
Kzo9I GATC 2 cut(s) 268, 731
LguI GCTCTTC 1 cut(s) 11
LmnI GCTCC 2 cut(s) 52, 388
Lsp1109I GCAGC 5 cut(s) 351, 392, 838, 949, 1116
LweI GCATC 3 cut(s) 616, 626, 799
MaeI CTAG 3 cut(s) 80, 1032, 1153
MaeII ACGT 1 cut(s) 1013
MaeIII GTNAC 3 cut(s) 177, 244, 397
MalI GATC 2 cut(s) 270, 733
MboI GATC 2 cut(s) 268, 731
MboII GAAGA 7 cut(s) 28, 38, 78, 479, 665, 701, 741
MfeI CAATTG 1 cut(s) 153
MflI RGATCY 1 cut(s) 731
MhlI GDGCHC 1 cut(s) 49
MluCI AATT 6 cut(s) 137, 153, 212, 519, 746, 1144
MlyI GAGTC 1 cut(s) 1130
MmeI TCCRAC 1 cut(s) 300
MroI TCCGGA 1 cut(s) 49
MseI TTAA 3 cut(s) 141, 768, 876
MslI CAYNNNNRTG 1 cut(s) 905
MspA1I CMGCKG 1 cut(s) 419
MspI CCGG 6 cut(s) 50, 61, 146, 444, 503, 756
MspR9I CCNGG 5 cut(s) 171, 369, 552, 738, 783
MunI CAATTG 1 cut(s) 153
Mva1269I GAATGC 1 cut(s) 994
MvaI CCWGG 5 cut(s) 171, 369, 552, 738, 783
MwoI GCNNNNNNNGC 9 cut(s) 129, 352, 361, 389, 540, 857, 968, 977, 1056
NdeII GATC 2 cut(s) 268, 731
NlaIII CATG 1 cut(s) 359
NmeAIII GCCGAG 1 cut(s) 1018
NmuCI GTSAC 2 cut(s) 244, 397
NspI RCATGY 1 cut(s) 359
PasI CCCWGGG 1 cut(s) 551
PciSI GCTCTTC 1 cut(s) 11
PcsI WCGNNNNNNNCGW 1 cut(s) 30
PctI GAATGC 1 cut(s) 994
PfeI GAWTC 3 cut(s) 10, 374, 425
PflMI CCANNNNNTGG 1 cut(s) 446
PfoI TCCNGGA 2 cut(s) 736, 781
PinAI ACCGGT 3 cut(s) 60, 443, 502
PkrI GCNGC 6 cut(s) 366, 382, 394, 853, 964, 1106
PleI GAGTC 1 cut(s) 1130
PpsI GAGTC 1 cut(s) 1130
PshAI GACNNNNGTC 1 cut(s) 102
Psp6I CCWGG 5 cut(s) 169, 367, 550, 736, 781
PspGI CCWGG 5 cut(s) 169, 367, 550, 736, 781
PspPI GGNCC 4 cut(s) 97, 554, 842, 953
PstI CTGCAG 2 cut(s) 382, 967
PsuI RGATCY 1 cut(s) 731
RsaI GTAC 2 cut(s) 68, 406
RsaNI GTAC 2 cut(s) 67, 405
RseI CAYNNNNRTG 1 cut(s) 905
SapI GCTCTTC 1 cut(s) 11
SaqAI TTAA 3 cut(s) 141, 768, 876
SatI GCNGC 6 cut(s) 365, 381, 393, 852, 963, 1105
Sau3AI GATC 2 cut(s) 268, 731
Sau96I GGNCC 4 cut(s) 97, 554, 842, 953
SchI GAGTC 1 cut(s) 1130
ScrFI CCNGG 5 cut(s) 171, 369, 552, 738, 783
SduI GDGCHC 1 cut(s) 49
SfaNI GCATC 3 cut(s) 616, 626, 799
SfcI CTRYAG 2 cut(s) 378, 963
SgrAI CRCCGGYG 1 cut(s) 502
SinI GGWCC 1 cut(s) 97
SmiMI CAYNNNNRTG 1 cut(s) 905
SpeI ACTAGT 1 cut(s) 1031
Sse9I AATT 6 cut(s) 137, 153, 212, 519, 746, 1144
SsiI CCGC 7 cut(s) 346, 393, 419, 590, 812, 1059, 1065
SspMI CTAG 3 cut(s) 80, 1032, 1153
StyD4I CCNGG 5 cut(s) 169, 367, 550, 736, 781
StyI CCWWGG 1 cut(s) 79
TaaI ACNGT 4 cut(s) 44, 104, 587, 698
TaiI ACGT 1 cut(s) 1016
TaqII GACCGA 1 cut(s) 71
TasI AATT 6 cut(s) 137, 153, 212, 519, 746, 1144
TatI WGTACW 1 cut(s) 404
TauI GCSGC 1 cut(s) 395
TfiI GAWTC 3 cut(s) 10, 374, 425
Tru1I TTAA 3 cut(s) 141, 768, 876
Tru9I TTAA 3 cut(s) 141, 768, 876
TscAI CASTG 6 cut(s) 345, 393, 907, 1057, 1114, 1129
TseFI GTSAC 2 cut(s) 244, 397
TseI GCWGC 5 cut(s) 364, 380, 851, 962, 1104
Tsp45I GTSAC 2 cut(s) 244, 397
TspDTI ATGAA 4 cut(s) 150, 657, 702, 1131
TspGWI ACGGA 1 cut(s) 246
TspRI CASTG 6 cut(s) 345, 393, 907, 1057, 1114, 1129
Van91I CCANNNNNTGG 1 cut(s) 446
VpaK11BI GGWCC 1 cut(s) 97
XagI CCTNNNNNAGG 1 cut(s) 780
XapI RAATTY 4 cut(s) 137, 212, 746, 1144
XceI RCATGY 1 cut(s) 359
XcmI CCANNNNNNNNNTGG 1 cut(s) 577
XmaJI CCTAGG 1 cut(s) 79
XspI CTAG 3 cut(s) 80, 1032, 1153
ZraI GACGTC 1 cut(s) 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.