pycom09g17720

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0020829
Physical Location & Seq
Forward (+)
18221607 .. 18222071
465 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g17720.1

Sequence Viewer

Length: 465 bp
ATGTCGAAGAACGATTCGACTTCGAAAGGCTCGGGAATCGGCGCCGCCGGCTCCATGGAGGAGCTGAAGAAGATCTTCAACAAGTTCGACAAGAACGGCGACGGCAGGATCTCCTGCGACGAGCTCAGGGGCATTTTCGGCGAGCTCGGCTCCGAGACGGCCTCCCTGGAGGAGGTGGAGCGCATTATGGCGGAGTTCGACAAGGACGGCGACGGCCACATTGACATCGCGGAGTTCGCCGAGATCATCAATGGCGGATCCACCAAGGAGCTTCGCGACGCGTTCGACCTATACGATCTTGACAAGAACGGGCTGATCTCCAAAAGTGAGCTGCACGAGGTGCTGAAGCGGCTGGGGCAGAAGTGCTCGCTCAAGGAGTGCGCGAAGATGATCGGCTCCGTCGACGCCGACGGCGATGGCCACGTCAACTTCGCCGAGTTCAAGAAGATGATGACTCGAAACTAA

Protein Analysis

155

Amino Acids

16.85

Weight (kDa)

4.89

Isoelectric Point (pI)

33.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 20 - 82 2.7e-14 EF-hand domain pair
EF-hand_1 PF00036 21 - 46 2.6e-08 EF hand domain
EF-hand_6 PF13405 21 - 50 2.2e-08 EF-hand domain
EF-hand_5 PF13202 22 - 43 1.6e-06 EF hand
EF-hand_1 PF00036 58 - 83 1.2e-07 EF hand domain
EF-hand_7 PF13499 89 - 152 2.1e-15 EF-hand domain pair
EF-hand_1 PF00036 90 - 118 3.3e-07 EF hand domain
EF-hand_6 PF13405 90 - 119 1.3e-07 EF-hand domain
EF-hand_8 PF13833 116 - 154 7.5e-06 EF-hand domain pair
EF-hand_1 PF00036 126 - 153 1.3e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020829)

Species Orthologous Gene IDs
malus_domestica MD09G1262900.v1.1 MD17G1257900.v1.1
prunus_persica Prupe.3G160600_v2.0.a1
pyrus_communis pycom09g17720 pycom17g25990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 41
AccI GTMKAC 1 cut(s) 402
AccII CGCG 4 cut(s) 230, 276, 281, 383
AciI CCGC 5 cut(s) 45, 191, 230, 255, 349
AclWI GGATC 3 cut(s) 116, 252, 265
AcoI YGGCCR 2 cut(s) 214, 418
AcuI CTGAAG 2 cut(s) 86, 365
AcyI GRCGYC 2 cut(s) 42, 405
AdeI CACNNNGTG 1 cut(s) 340
AfiI CCNNNNNNNGG 1 cut(s) 172
AflIII ACRYGT 1 cut(s) 279
AgsI TTSAA 2 cut(s) 79, 442
AjiI CACGTC 1 cut(s) 424
AjnI CCWGG 1 cut(s) 165
AluBI AGCT 5 cut(s) 64, 124, 145, 271, 331
AluI AGCT 5 cut(s) 64, 124, 145, 271, 331
Alw21I GWGCWC 3 cut(s) 126, 147, 368
Alw26I GTCTC 1 cut(s) 149
AlwI GGATC 3 cut(s) 116, 252, 265
Ama87I CYCGRG 1 cut(s) 31
AoxI GGCC 3 cut(s) 159, 214, 418
ApeKI GCWGC 1 cut(s) 331
Asp700I GAANNNNTTC 1 cut(s) 74
AspLEI GCGC 3 cut(s) 44, 183, 383
AsuII TTCGAA 1 cut(s) 23
AvaI CYCGRG 1 cut(s) 31
BalI TGGCCA 1 cut(s) 420
BamHI GGATCC 1 cut(s) 257
BanI GGYRCC 1 cut(s) 41
BanII GRGCYC 2 cut(s) 126, 147
BauI CACGAG 1 cut(s) 335
Bbv12I GWGCWC 3 cut(s) 126, 147, 368
BbvI GCAGC 1 cut(s) 318
BccI CCATC 1 cut(s) 410
BceAI ACGGC 6 cut(s) 112, 118, 174, 223, 229, 427
BciT130I CCWGG 1 cut(s) 167
BcoDI GTCTC 1 cut(s) 149
BfoI RGCGCY 1 cut(s) 45
BglII AGATCT 1 cut(s) 72
BisI GCNGC 3 cut(s) 45, 332, 350
BlsI GCNGC 3 cut(s) 46, 333, 351
Bme1390I CCNGG 1 cut(s) 167
BmeT110I CYCGRG 1 cut(s) 31
BmgBI CACGTC 1 cut(s) 424
BmiI GGNNCC 5 cut(s) 43, 52, 151, 259, 397
BmrFI CCNGG 1 cut(s) 167
BplI GAGNNNNNCTC 4 cut(s) 134, 166, 146, 178
BpmI CTGGAG 1 cut(s) 188
Bpu10I CCTNAGC 1 cut(s) 125
Bpu14I TTCGAA 1 cut(s) 23
BpuEI CTTGAG 1 cut(s) 356
BsaHI GRCGYC 2 cut(s) 42, 405
BsaJI CCNNGG 3 cut(s) 54, 165, 264
Bsc4I CCNNNNNNNGG 1 cut(s) 172
Bse118I RCCGGY 1 cut(s) 47
BseBI CCWGG 1 cut(s) 167
BseDI CCNNGG 3 cut(s) 54, 165, 264
BseLI CCNNNNNNNGG 1 cut(s) 172
BseMII CTCAG 1 cut(s) 139
BseRI GAGGAG 2 cut(s) 74, 185
BseXI GCAGC 1 cut(s) 318
BseYI CCCAGC 1 cut(s) 352
BsgI GTGCAG 1 cut(s) 317
Bsh1236I CGCG 4 cut(s) 230, 276, 281, 383
BshFI GGCC 3 cut(s) 161, 216, 420
BshNI GGYRCC 1 cut(s) 41
BsiHKAI GWGCWC 3 cut(s) 126, 147, 368
BsiHKCI CYCGRG 1 cut(s) 31
BsiSI CCGG 1 cut(s) 48
BslI CCNNNNNNNGG 1 cut(s) 172
BsmAI GTCTC 1 cut(s) 149
BsmBI CGTCTC 1 cut(s) 149
BsnI GGCC 3 cut(s) 161, 216, 420
BsoBI CYCGRG 1 cut(s) 31
Bsp119I TTCGAA 1 cut(s) 23
Bsp1286I GDGCHC 3 cut(s) 126, 147, 368
Bsp143I GATC 7 cut(s) 72, 108, 243, 257, 295, 315, 390
Bsp19I CCATGG 1 cut(s) 54
Bsp68I TCGCGA 1 cut(s) 276
BspACI CCGC 5 cut(s) 45, 191, 230, 255, 349
BspANI GGCC 3 cut(s) 161, 216, 420
BspCNI CTCAG 1 cut(s) 138
BspFNI CGCG 4 cut(s) 230, 276, 281, 383
BspLI GGNNCC 5 cut(s) 43, 52, 151, 259, 397
BspPI GGATC 3 cut(s) 116, 252, 265
BspT104I TTCGAA 1 cut(s) 23
BspT107I GGYRCC 1 cut(s) 41
BsrFI RCCGGY 1 cut(s) 47
BssAI RCCGGY 1 cut(s) 47
BssECI CCNNGG 3 cut(s) 54, 165, 264
BssMI GATC 7 cut(s) 72, 108, 243, 257, 295, 315, 390
BssNI GRCGYC 2 cut(s) 42, 405
BssSI CACGAG 1 cut(s) 335
BssT1I CCWWGG 2 cut(s) 54, 264
Bst2BI CACGAG 1 cut(s) 335
Bst2UI CCWGG 1 cut(s) 167
BstACI GRCGYC 2 cut(s) 42, 405
BstAPI GCANNNNNTGC 1 cut(s) 340
BstBI TTCGAA 1 cut(s) 23
BstC8I GCNNGC 3 cut(s) 49, 143, 368
BstDEI CTNAG 1 cut(s) 125
BstDSI CCRYGG 1 cut(s) 54
BstENI CCTNNNNNAGG 1 cut(s) 170
BstFNI CGCG 4 cut(s) 230, 276, 281, 383
BstH2I RGCGCY 1 cut(s) 45
BstHHI GCGC 3 cut(s) 44, 183, 383
BstKTI GATC 7 cut(s) 75, 111, 246, 260, 298, 318, 393
BstMAI GTCTC 1 cut(s) 149
BstMBI GATC 7 cut(s) 72, 108, 243, 257, 295, 315, 390
BstMWI GCNNNNNNNGC 7 cut(s) 48, 138, 147, 236, 340, 349, 355
BstNI CCWGG 1 cut(s) 167
BstSCI CCNGG 1 cut(s) 165
BstUI CGCG 4 cut(s) 230, 276, 281, 383
BstV1I GCAGC 1 cut(s) 318
BstX2I RGATCY 3 cut(s) 72, 108, 257
BstYI RGATCY 3 cut(s) 72, 108, 257
BsuRI GGCC 3 cut(s) 161, 216, 420
BtgI CCRYGG 1 cut(s) 54
BtgZI GCGATG 2 cut(s) 211, 429
BtrI CACGTC 1 cut(s) 424
BtuMI TCGCGA 1 cut(s) 276
Cac8I GCNNGC 3 cut(s) 49, 143, 368
CfoI GCGC 3 cut(s) 44, 183, 383
Cfr10I RCCGGY 1 cut(s) 47
CseI GACGC 2 cut(s) 287, 413
CviAII CATG 1 cut(s) 55
DdeI CTNAG 1 cut(s) 125
DinI GGCGCC 1 cut(s) 43
DpnI GATC 7 cut(s) 74, 110, 245, 259, 297, 317, 392
DpnII GATC 7 cut(s) 72, 108, 243, 257, 295, 315, 390
DraIII CACNNNGTG 1 cut(s) 340
EaeI YGGCCR 2 cut(s) 214, 418
EciI GGCGGA 2 cut(s) 206, 270
Ecl136II GAGCTC 2 cut(s) 124, 145
Eco130I CCWWGG 2 cut(s) 54, 264
Eco24I GRGCYC 2 cut(s) 126, 147
Eco53kI GAGCTC 2 cut(s) 124, 145
Eco57I CTGAAG 2 cut(s) 86, 365
Eco88I CYCGRG 1 cut(s) 31
EcoICRI GAGCTC 2 cut(s) 124, 145
EcoNI CCTNNNNNAGG 1 cut(s) 170
EcoRII CCWGG 1 cut(s) 165
EcoT14I CCWWGG 2 cut(s) 54, 264
EcoT38I GRGCYC 2 cut(s) 126, 147
EgeI GGCGCC 1 cut(s) 43
EheI GGCGCC 1 cut(s) 43
ErhI CCWWGG 2 cut(s) 54, 264
Esp3I CGTCTC 1 cut(s) 149
FaeI CATG 1 cut(s) 58
FaiI YATR 3 cut(s) 56, 188, 292
FalI AAGNNNNNCTT 2 cut(s) 59, 91
FatI CATG 1 cut(s) 54
FblI GTMKAC 1 cut(s) 402
Fnu4HI GCNGC 3 cut(s) 45, 332, 350
FriOI GRGCYC 2 cut(s) 126, 147
Fsp4HI GCNGC 3 cut(s) 45, 332, 350
GlaI GCGC 3 cut(s) 43, 182, 382
GluI GCNGC 3 cut(s) 45, 332, 350
GsaI CCCAGC 1 cut(s) 356
GsuI CTGGAG 1 cut(s) 188
HaeII RGCGCY 1 cut(s) 45
HaeIII GGCC 3 cut(s) 161, 216, 420
HapII CCGG 1 cut(s) 48
HgaI GACGC 2 cut(s) 287, 413
HhaI GCGC 3 cut(s) 44, 183, 383
Hin1I GRCGYC 2 cut(s) 42, 405
Hin1II CATG 1 cut(s) 58
Hin6I GCGC 3 cut(s) 42, 181, 381
HinP1I GCGC 3 cut(s) 42, 181, 381
HincII GTYRAC 2 cut(s) 403, 427
HindII GTYRAC 2 cut(s) 403, 427
HinfI GANTC 3 cut(s) 14, 36, 454
HpaII CCGG 1 cut(s) 48
Hpy166II GTNNAC 2 cut(s) 403, 427
Hpy188I TCNGA 1 cut(s) 154
Hpy188III TCNNGA 4 cut(s) 33, 275, 299, 442
Hpy8I GTNNAC 2 cut(s) 403, 427
Hpy99I CGWCG 7 cut(s) 104, 122, 215, 281, 404, 407, 413
HpyCH4IV ACGT 1 cut(s) 423
HpyCH4V TGCA 1 cut(s) 334
HpyF10VI GCNNNNNNNGC 7 cut(s) 48, 138, 147, 236, 340, 349, 355
HpyF3I CTNAG 1 cut(s) 125
HpySE526I ACGT 1 cut(s) 423
Hsp92I GRCGYC 2 cut(s) 42, 405
Hsp92II CATG 1 cut(s) 58
HspAI GCGC 3 cut(s) 42, 181, 381
KasI GGCGCC 1 cut(s) 41
KroI GCCGGC 1 cut(s) 47
KroNI GCCGGC 1 cut(s) 49
Kzo9I GATC 7 cut(s) 72, 108, 243, 257, 295, 315, 390
LmnI GCTCC 6 cut(s) 56, 61, 155, 178, 268, 401
LpnPI CCDG 7 cut(s) 61, 91, 112, 127, 152, 179, 338
Lsp1109I GCAGC 1 cut(s) 318
MaeII ACGT 1 cut(s) 423
MalI GATC 7 cut(s) 74, 110, 245, 259, 297, 317, 392
MboI GATC 7 cut(s) 72, 108, 243, 257, 295, 315, 390
MboII GAAGA 6 cut(s) 19, 67, 79, 82, 397, 457
MflI RGATCY 3 cut(s) 72, 108, 257
MhlI GDGCHC 3 cut(s) 126, 147, 368
MlsI TGGCCA 1 cut(s) 420
MluI ACGCGT 1 cut(s) 279
MluNI TGGCCA 1 cut(s) 420
Mly113I GGCGCC 1 cut(s) 42
MlyI GAGTC 1 cut(s) 448
MnlI CCTC 5 cut(s) 52, 163, 166, 172, 331
Mox20I TGGCCA 1 cut(s) 420
MroNI GCCGGC 1 cut(s) 47
MroXI GAANNNNTTC 1 cut(s) 74
MscI TGGCCA 1 cut(s) 420
Msp20I TGGCCA 1 cut(s) 420
MspI CCGG 1 cut(s) 48
MspR9I CCNGG 1 cut(s) 167
MvaI CCWGG 1 cut(s) 167
MvnI CGCG 4 cut(s) 230, 276, 281, 383
MwoI GCNNNNNNNGC 7 cut(s) 48, 138, 147, 236, 340, 349, 355
NaeI GCCGGC 1 cut(s) 49
NarI GGCGCC 1 cut(s) 42
NcoI CCATGG 1 cut(s) 54
NdeII GATC 7 cut(s) 72, 108, 243, 257, 295, 315, 390
NgoMIV GCCGGC 1 cut(s) 47
NlaIII CATG 1 cut(s) 58
NlaIV GGNNCC 5 cut(s) 43, 52, 151, 259, 397
NmeAIII GCCGAG 3 cut(s) 126, 265, 460
NruI TCGCGA 1 cut(s) 276
NspV TTCGAA 1 cut(s) 23
PcsI WCGNNNNNNNCGW 3 cut(s) 291, 399, 411
PdiI GCCGGC 1 cut(s) 49
PdmI GAANNNNTTC 1 cut(s) 74
PfeI GAWTC 2 cut(s) 14, 36
PkrI GCNGC 3 cut(s) 46, 333, 351
PleI GAGTC 1 cut(s) 448
PluTI GGCGCC 1 cut(s) 45
PpsI GAGTC 1 cut(s) 448
Psp124BI GAGCTC 2 cut(s) 126, 147
Psp6I CCWGG 1 cut(s) 165
PspFI CCCAGC 1 cut(s) 352
PspGI CCWGG 1 cut(s) 165
PspN4I GGNNCC 5 cut(s) 43, 52, 151, 259, 397
PsuI RGATCY 3 cut(s) 72, 108, 257
RruI TCGCGA 1 cut(s) 276
SacI GAGCTC 2 cut(s) 126, 147
SalI GTCGAC 1 cut(s) 401
SatI GCNGC 3 cut(s) 45, 332, 350
Sau3AI GATC 7 cut(s) 72, 108, 243, 257, 295, 315, 390
SchI GAGTC 1 cut(s) 448
ScrFI CCNGG 1 cut(s) 167
SduI GDGCHC 3 cut(s) 126, 147, 368
SetI ASST 9 cut(s) 66, 126, 147, 177, 273, 291, 333, 342, 426
SfoI GGCGCC 1 cut(s) 43
SfuI TTCGAA 1 cut(s) 23
SgrDI CGTCGACG 1 cut(s) 401
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
SsiI CCGC 5 cut(s) 45, 191, 230, 255, 349
SspDI GGCGCC 1 cut(s) 41
SstI GAGCTC 2 cut(s) 126, 147
StyD4I CCNGG 1 cut(s) 165
StyI CCWWGG 2 cut(s) 54, 264
TaiI ACGT 1 cut(s) 426
TaqI TCGA 8 cut(s) 5, 17, 23, 87, 198, 285, 402, 457
TauI GCSGC 2 cut(s) 47, 352
TfiI GAWTC 2 cut(s) 14, 36
TseI GCWGC 1 cut(s) 331
TspGWI ACGGA 1 cut(s) 388
XagI CCTNNNNNAGG 1 cut(s) 170
XmiI GTMKAC 1 cut(s) 402
XmnI GAANNNNTTC 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.