pycom10g10300

glomerular visceral epithelial cell migration

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0028201
Physical Location & Seq
Forward (+)
13607132 .. 13608637
1506 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1374 bp
ATGGGGGTTGACACTGTAGTCAAGCTCTTACTTATGTTGCTTCTCTCAGCGGTGGAGTTTAGTTGTCATAAGTTGCATTTGGTTGGGACAAGAGATATAGTTTCTGTCGAAGACATGGCTAAAATATCTAATGTGAGTCTTGGTCTACTACCAGTTCTCTGTAACTGTATTGCTACTGCTGAGCATGGAACCCTCTCCCTAACTACTGTGGACTTGATACTTAGAAATTTCTTGACTCCCAACACTTGGTTCCCCATTATACAGAACCACCTCCAGCTTCAGCATGTGATTCTGAAGCTTCAAAATAAAAATGCTCTTGAATCTGTGCCTGTTATAATGAAGTTCTTTATGACTCTTGCCCGTGTTAGGCAGGGTGCAGAAATGCTTATTAATTATGGCTTTCTCTCGTCTCTGAGATTCTTGTTCGCTGGATACTTGGATGGTACGTCTTCTTCCGAAACTATTGATAACAGAAATTCCAACTCATCATCTGAGAAACTGGAAAAGCCTCGGCAAATTTGGGGACTTGGTTTGGCAGTCATCACAGTTATGGTTGAGTCATTGGGAGACAGTTCTGCTTGTTCCAATCTTGTGGAGAATGTAATACCTTACTTCTTTTCAGAGAAAGACCATATGATTTCTTACTATCTAAGTGCTCCGGATTTCCCATCTGATGATCATGACAAGAAAAGACCTTGGGCTCAGCAGAGACAAACTTCTCATACTGATCTTAAAGAAACTGAGCACACTCTCATGCTAATGTGTGTGCTAGCAAAACATTGGAATTCCTGGGTTAAGTCCATGAAAGAATTGGATTCCCAGTTGAGAGAGAAAAGTATCCGTCTTTTAGCCTTTATCAGTCGGGGAACTCAATGGCTTGGAGAACCTTCTAGTCTTAGTGCCCCTCTGTTATGTCCTTCTGTCCTTAAAGAGGAATTTGATGGCTGGAAGAAACCCTCATTTATGAACAGTAAAAGCGGATGGTTTGCTCTTTCAGCCCTCAGTTGTGTTTCAAAACCAAAATTCTCCACCATCCCTACCACATCTACTGCGCTGGTTATTAAAACTCAAGCAACTGTAAATGGCAGTCACATTTCTCAGTCATATTTCTCCGACTCTATTGCCCTACAGATCTACAGAATAACTTTTCTCCTTTTGAAGTTCCTGAGCTTGCAAGCTGAGGGTGCTGCTAGAAGGGAAGAAGAGGTTGGTTTTGTAGATCTTGACCATTTTCCTGAGCTTCCAATGCCTGATCGATCTTACATGGCTTACAGGACCAAGCAATTACAATTGTTACAGAGCTGTGTGTGGTTAACAGATCAAACGAGATTCAAATTGAAGTCCAGAGTATCTGTTGCTTACTGCTACAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Nup188

Protein Analysis

458

Amino Acids

51.63

Weight (kDa)

8.45

Isoelectric Point (pI)

40.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028201)

Species Orthologous Gene IDs
malus_domestica MD10G1119600.v1.1
pyrus_communis pycom10g10300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 335
AasI GACNNNNNNGTC 1 cut(s) 17
AccB7I CCANNNNNTGG 1 cut(s) 246
AccI GTMKAC 1 cut(s) 145
AccIII TCCGGA 1 cut(s) 658
AciI CCGC 2 cut(s) 50, 978
AcsI RAATTY 6 cut(s) 226, 475, 516, 784, 935, 1022
AcuI CTGAAG 2 cut(s) 263, 314
AfaI GTAC 1 cut(s) 445
AfiI CCNNNNNNNGG 3 cut(s) 246, 366, 931
AgsI TTSAA 6 cut(s) 302, 320, 1014, 1159, 1333, 1339
AjnI CCWGG 1 cut(s) 788
AjuI GAANNNNNNNTTGG 4 cut(s) 233, 265, 1191, 1223
AluBI AGCT 7 cut(s) 25, 277, 298, 1170, 1178, 1240, 1302
AluI AGCT 7 cut(s) 25, 277, 298, 1170, 1178, 1240, 1302
Alw21I GWGCWC 2 cut(s) 658, 747
Alw26I GTCTC 3 cut(s) 414, 561, 703
Aor13HI TCCGGA 1 cut(s) 658
ApeKI GCWGC 1 cut(s) 1187
ApoI RAATTY 6 cut(s) 226, 475, 516, 784, 935, 1022
AseI ATTAAT 1 cut(s) 390
AspLEI GCGC 1 cut(s) 1054
AspS9I GGNCC 1 cut(s) 1275
AsuNHI GCTAGC 1 cut(s) 769
AvaII GGWCC 1 cut(s) 1275
BaeGI GKGCMC 1 cut(s) 904
BanII GRGCYC 1 cut(s) 703
BarI GAAGNNNNNNTAC 2 cut(s) 436, 468
BbsI GAAGAC 2 cut(s) 117, 441
Bbv12I GWGCWC 2 cut(s) 658, 747
BbvCI CCTCAGC 1 cut(s) 1179
BbvI GCAGC 1 cut(s) 1174
BccI CCATC 5 cut(s) 434, 676, 935, 975, 1040
BcgI CGANNNNNNTGC 2 cut(s) 1103, 1137
BciT130I CCWGG 1 cut(s) 790
BciVI GTATCC 2 cut(s) 425, 848
BclI TGATCA 1 cut(s) 676
BcoDI GTCTC 3 cut(s) 414, 561, 703
BfaI CTAG 3 cut(s) 770, 891, 1191
BfmI CTRYAG 3 cut(s) 15, 1127, 1135
BfuI GTATCC 2 cut(s) 425, 848
BglII AGATCT 2 cut(s) 1131, 1219
BisI GCNGC 1 cut(s) 1188
BlpI GCTNAGC 2 cut(s) 180, 702
BlsI GCNGC 1 cut(s) 1189
Bme1390I CCNGG 1 cut(s) 790
Bme18I GGWCC 1 cut(s) 1275
BmgT120I GGNCC 1 cut(s) 1275
BmiI GGNNCC 2 cut(s) 190, 251
BmrFI CCNGG 1 cut(s) 790
BmrI ACTGGG 1 cut(s) 814
BmtI GCTAGC 1 cut(s) 773
BmuI ACTGGG 1 cut(s) 814
BpiI GAAGAC 2 cut(s) 117, 441
BpmI CTGGAG 1 cut(s) 257
Bpu10I CCTNAGC 3 cut(s) 1166, 1179, 1236
Bpu1102I GCTNAGC 2 cut(s) 180, 702
BpuEI CTTGAG 1 cut(s) 1053
Bsa29I ATCGAT 1 cut(s) 1255
BsaJI CCNNGG 3 cut(s) 509, 695, 789
BsaWI WCCGGW 1 cut(s) 658
Bsc4I CCNNNNNNNGG 3 cut(s) 246, 366, 931
Bse1I ACTGG 3 cut(s) 152, 504, 820
BseAI TCCGGA 1 cut(s) 658
BseBI CCWGG 1 cut(s) 790
BseCI ATCGAT 1 cut(s) 1255
BseDI CCNNGG 3 cut(s) 509, 695, 789
BseGI GGATG 3 cut(s) 445, 986, 1032
BseLI CCNNNNNNNGG 3 cut(s) 246, 366, 931
BseNI ACTGG 3 cut(s) 152, 504, 820
BseSI GKGCMC 1 cut(s) 904
BseXI GCAGC 1 cut(s) 1174
BsgI GTGCAG 1 cut(s) 396
BshVI ATCGAT 1 cut(s) 1255
BsiHKAI GWGCWC 2 cut(s) 658, 747
BsiSI CCGG 1 cut(s) 659
BslFI GGGAC 2 cut(s) 100, 537
BslI CCNNNNNNNGG 3 cut(s) 246, 366, 931
BsmAI GTCTC 3 cut(s) 414, 561, 703
BsmBI CGTCTC 1 cut(s) 414
BsmFI GGGAC 2 cut(s) 100, 537
Bsp1286I GDGCHC 4 cut(s) 658, 703, 747, 904
Bsp13I TCCGGA 1 cut(s) 658
Bsp143I GATC 7 cut(s) 676, 727, 1131, 1219, 1252, 1256, 1318
Bsp1720I GCTNAGC 2 cut(s) 180, 702
BspACI CCGC 2 cut(s) 50, 978
BspDI ATCGAT 1 cut(s) 1255
BspEI TCCGGA 1 cut(s) 658
BspHI TCATGA 1 cut(s) 679
BspLI GGNNCC 2 cut(s) 190, 251
BspOI GCTAGC 1 cut(s) 773
BsrI ACTGG 3 cut(s) 152, 504, 820
BssECI CCNNGG 3 cut(s) 509, 695, 789
BssMI GATC 7 cut(s) 676, 727, 1131, 1219, 1252, 1256, 1318
BssT1I CCWWGG 1 cut(s) 695
Bst2UI CCWGG 1 cut(s) 790
Bst4CI ACNGT 7 cut(s) 16, 167, 208, 547, 572, 971, 1078
Bst6I CTCTTC 1 cut(s) 1197
BstC8I GCNNGC 3 cut(s) 771, 1172, 1176
BstENI CCTNNNNNAGG 1 cut(s) 929
BstF5I GGATG 3 cut(s) 445, 986, 1032
BstHHI GCGC 1 cut(s) 1054
BstKTI GATC 7 cut(s) 679, 730, 1134, 1222, 1255, 1259, 1321
BstMAI GTCTC 3 cut(s) 414, 561, 703
BstMBI GATC 7 cut(s) 676, 727, 1131, 1219, 1252, 1256, 1318
BstMWI GCNNNNNNNGC 3 cut(s) 995, 1184, 1246
BstNI CCWGG 1 cut(s) 790
BstNSI RCATGY 1 cut(s) 287
BstSCI CCNGG 1 cut(s) 788
BstSFI CTRYAG 3 cut(s) 15, 1127, 1135
BstSLI GKGCMC 1 cut(s) 904
BstV1I GCAGC 1 cut(s) 1174
BstV2I GAAGAC 2 cut(s) 117, 441
BstX2I RGATCY 2 cut(s) 1131, 1219
BstXI CCANNNNNNTGG 1 cut(s) 592
BstYI RGATCY 2 cut(s) 1131, 1219
Bsu15I ATCGAT 1 cut(s) 1255
BsuI GTATCC 2 cut(s) 425, 848
BsuTUI ATCGAT 1 cut(s) 1255
BtsCI GGATG 3 cut(s) 445, 986, 1032
BtsIMutI CAGTG 1 cut(s) 12
Cac8I GCNNGC 3 cut(s) 771, 1172, 1176
CciI TCATGA 1 cut(s) 679
CfoI GCGC 1 cut(s) 1054
Cfr13I GGNCC 1 cut(s) 1275
ClaI ATCGAT 1 cut(s) 1255
Csp6I GTAC 1 cut(s) 444
CviAII CATG 7 cut(s) 115, 185, 284, 680, 754, 802, 1264
CviQI GTAC 1 cut(s) 444
DpnI GATC 7 cut(s) 678, 729, 1133, 1221, 1254, 1258, 1320
DpnII GATC 7 cut(s) 676, 727, 1131, 1219, 1252, 1256, 1318
DrdI GACNNNNNNGTC 1 cut(s) 17
DseDI GACNNNNNNGTC 1 cut(s) 17
Eam1104I CTCTTC 1 cut(s) 1197
EarI CTCTTC 1 cut(s) 1197
Eco130I CCWWGG 1 cut(s) 695
Eco24I GRGCYC 1 cut(s) 703
Eco47I GGWCC 1 cut(s) 1275
Eco57I CTGAAG 2 cut(s) 263, 314
EcoNI CCTNNNNNAGG 1 cut(s) 929
EcoRI GAATTC 1 cut(s) 784
EcoRII CCWGG 1 cut(s) 788
EcoT14I CCWWGG 1 cut(s) 695
EcoT38I GRGCYC 1 cut(s) 703
ErhI CCWWGG 1 cut(s) 695
Esp3I CGTCTC 1 cut(s) 414
FaeI CATG 7 cut(s) 118, 188, 287, 683, 757, 805, 1267
FaqI GGGAC 2 cut(s) 100, 537
FatI CATG 7 cut(s) 114, 184, 283, 679, 753, 801, 1263
FauNDI CATATG 1 cut(s) 633
FbaI TGATCA 1 cut(s) 676
FblI GTMKAC 1 cut(s) 145
Fnu4HI GCNGC 1 cut(s) 1188
FokI GGATG 3 cut(s) 452, 993, 1019
FriOI GRGCYC 1 cut(s) 703
Fsp4HI GCNGC 1 cut(s) 1188
FspBI CTAG 3 cut(s) 770, 891, 1191
GlaI GCGC 1 cut(s) 1053
GluI GCNGC 1 cut(s) 1188
GsuI CTGGAG 1 cut(s) 257
HapII CCGG 1 cut(s) 659
HhaI GCGC 1 cut(s) 1054
Hin1II CATG 7 cut(s) 118, 188, 287, 683, 757, 805, 1267
Hin6I GCGC 1 cut(s) 1052
HinP1I GCGC 1 cut(s) 1052
HincII GTYRAC 2 cut(s) 10, 1314
HindII GTYRAC 2 cut(s) 10, 1314
HindIII AAGCTT 1 cut(s) 296
HpaI GTTAAC 1 cut(s) 1314
HpaII CCGG 1 cut(s) 659
Hpy166II GTNNAC 4 cut(s) 10, 146, 211, 1314
Hpy188I TCNGA 7 cut(s) 294, 414, 457, 493, 622, 673, 1114
Hpy188III TCNNGA 8 cut(s) 232, 317, 659, 680, 1165, 1223, 1235, 1344
Hpy8I GTNNAC 4 cut(s) 10, 146, 211, 1314
HpyAV CCTTC 3 cut(s) 897, 927, 1188
HpyCH4III ACNGT 7 cut(s) 16, 167, 208, 547, 572, 971, 1078
HpyCH4IV ACGT 1 cut(s) 446
HpyCH4V TGCA 3 cut(s) 76, 377, 1174
HpyF10VI GCNNNNNNNGC 3 cut(s) 995, 1184, 1246
HpySE526I ACGT 1 cut(s) 446
Hsp92II CATG 7 cut(s) 118, 188, 287, 683, 757, 805, 1267
HspAI GCGC 1 cut(s) 1052
Kpn2I TCCGGA 1 cut(s) 658
Ksp22I TGATCA 1 cut(s) 676
KspAI GTTAAC 1 cut(s) 1314
Kzo9I GATC 7 cut(s) 676, 727, 1131, 1219, 1252, 1256, 1318
LmnI GCTCC 1 cut(s) 661
Lsp1109I GCAGC 1 cut(s) 1174
MaeI CTAG 3 cut(s) 770, 891, 1191
MaeII ACGT 1 cut(s) 446
MaeIII GTNAC 3 cut(s) 161, 1088, 1293
MalI GATC 7 cut(s) 678, 729, 1133, 1221, 1254, 1258, 1320
MboI GATC 7 cut(s) 676, 727, 1131, 1219, 1252, 1256, 1318
MboII GAAGA 6 cut(s) 122, 441, 444, 961, 1211, 1214
MfeI CAATTG 1 cut(s) 1289
MflI RGATCY 2 cut(s) 1131, 1219
MhlI GDGCHC 4 cut(s) 658, 703, 747, 904
MlyI GAGTC 5 cut(s) 145, 229, 346, 566, 1109
MmeI TCCRAC 2 cut(s) 504, 1137
MnlI CCTC 9 cut(s) 203, 281, 519, 915, 925, 967, 1010, 1174, 1198
MroI TCCGGA 1 cut(s) 658
MseI TTAA 6 cut(s) 390, 732, 795, 927, 1062, 1313
MslI CAYNNNNRTG 3 cut(s) 548, 752, 758
MspA1I CMGCKG 1 cut(s) 50
MspI CCGG 1 cut(s) 659
MspR9I CCNGG 1 cut(s) 790
MunI CAATTG 1 cut(s) 1289
MvaI CCWGG 1 cut(s) 790
MwoI GCNNNNNNNGC 3 cut(s) 995, 1184, 1246
NdeI CATATG 1 cut(s) 633
NdeII GATC 7 cut(s) 676, 727, 1131, 1219, 1252, 1256, 1318
NheI GCTAGC 1 cut(s) 769
NlaIII CATG 7 cut(s) 118, 188, 287, 683, 757, 805, 1267
NlaIV GGNNCC 2 cut(s) 190, 251
NmeAIII GCCGAG 1 cut(s) 490
NmuCI GTSAC 1 cut(s) 1088
NspI RCATGY 1 cut(s) 287
PagI TCATGA 1 cut(s) 679
PfeI GAWTC 5 cut(s) 289, 320, 417, 815, 1329
PflMI CCANNNNNTGG 1 cut(s) 246
PkrI GCNGC 1 cut(s) 1189
PleI GAGTC 5 cut(s) 144, 229, 346, 565, 1109
PpsI GAGTC 5 cut(s) 144, 229, 346, 565, 1109
PshBI ATTAAT 1 cut(s) 390
PsiI TTATAA 1 cut(s) 335
Psp6I CCWGG 1 cut(s) 788
PspGI CCWGG 1 cut(s) 788
PspN4I GGNNCC 2 cut(s) 190, 251
PspPI GGNCC 1 cut(s) 1275
PsuI RGATCY 2 cut(s) 1131, 1219
RsaI GTAC 1 cut(s) 445
RsaNI GTAC 1 cut(s) 444
RseI CAYNNNNRTG 3 cut(s) 548, 752, 758
SaqAI TTAA 6 cut(s) 390, 732, 795, 927, 1062, 1313
SatI GCNGC 1 cut(s) 1188
Sau3AI GATC 7 cut(s) 676, 727, 1131, 1219, 1252, 1256, 1318
Sau96I GGNCC 1 cut(s) 1275
SchI GAGTC 5 cut(s) 145, 229, 346, 566, 1109
ScrFI CCNGG 1 cut(s) 790
SduI GDGCHC 4 cut(s) 658, 703, 747, 904
SfcI CTRYAG 3 cut(s) 15, 1127, 1135
SinI GGWCC 1 cut(s) 1275
SmiMI CAYNNNNRTG 3 cut(s) 548, 752, 758
SmlI CTYRAG 1 cut(s) 1068
SmoI CTYRAG 1 cut(s) 1068
SsiI CCGC 2 cut(s) 50, 978
SspMI CTAG 3 cut(s) 770, 891, 1191
StyD4I CCNGG 1 cut(s) 788
StyI CCWWGG 1 cut(s) 695
TaaI ACNGT 7 cut(s) 16, 167, 208, 547, 572, 971, 1078
TaiI ACGT 1 cut(s) 449
TaqI TCGA 2 cut(s) 108, 1255
TfiI GAWTC 5 cut(s) 289, 320, 417, 815, 1329
Tru1I TTAA 6 cut(s) 390, 732, 795, 927, 1062, 1313
Tru9I TTAA 6 cut(s) 390, 732, 795, 927, 1062, 1313
TscAI CASTG 1 cut(s) 19
TseFI GTSAC 1 cut(s) 1088
TseI GCWGC 1 cut(s) 1187
Tsp45I GTSAC 1 cut(s) 1088
TspDTI ATGAA 3 cut(s) 353, 818, 980
TspGWI ACGGA 1 cut(s) 830
TspRI CASTG 1 cut(s) 19
Van91I CCANNNNNTGG 1 cut(s) 246
VpaK11BI GGWCC 1 cut(s) 1275
VspI ATTAAT 1 cut(s) 390
XagI CCTNNNNNAGG 1 cut(s) 929
XapI RAATTY 6 cut(s) 226, 475, 516, 784, 935, 1022
XceI RCATGY 1 cut(s) 287
XcmI CCANNNNNNNNNTGG 1 cut(s) 808
XmiI GTMKAC 1 cut(s) 145
XspI CTAG 3 cut(s) 770, 891, 1191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.