pycom10g10390

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0024667
Physical Location & Seq
Forward (+)
13661419 .. 13661850
432 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 432 bp
ATGTCAAATTTAGCAAGACTGTCCAGAGATGGATTCGAGAAGTTGATGAAAGATGAGAACCTAGACGCGTTGGTGACTTATTCCAACTCTGCTCATTCAATCCTTGGAATTGGTGGTTTTCCAGGCATTGTTGTGCCGGCAGGATATCATAACGATGACAAGTACCCTTTCGGCATTTGCTTTGGGGGACTAAAGGGTTCGGAGCCAAAGCTGATTGAGGTTGCGTATGCATTTGAGCAAGCAACTAAGATCAGAAAGCCTCCGCCCGTTGGTTCTCAAGGCCAACTCCTCTCTACCACCGCTACTCCCACTCCTGAAGTTGAGTCATCAATACCCACCATTGCTGCAGCCACAAGAAGACCCGTCCTAGTCACTCAAGAGAACCTCCAAGGAAAGCGCTTGCATGACAGCAGTCGACATGAAGTTCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

144

Amino Acids

15.43

Weight (kDa)

6.91

Isoelectric Point (pI)

51.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024667)

Species Orthologous Gene IDs
pyrus_communis pycom10g10390
rosa_multiflora Rmu_sc0007349.1_g000002 Rmu_sc0022734.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 415
AccII CGCG 1 cut(s) 68
AciI CCGC 2 cut(s) 263, 300
AcsI RAATTY 1 cut(s) 7
AcuI CTGAAG 1 cut(s) 336
AfaI GTAC 1 cut(s) 164
AfeI AGCGCT 1 cut(s) 398
AfiI CCNNNNNNNGG 1 cut(s) 269
AflIII ACRYGT 1 cut(s) 66
AgsI TTSAA 1 cut(s) 99
AjnI CCWGG 1 cut(s) 121
AluBI AGCT 1 cut(s) 211
AluI AGCT 1 cut(s) 211
Aor51HI AGCGCT 1 cut(s) 398
AoxI GGCC 1 cut(s) 280
ApeKI GCWGC 2 cut(s) 344, 347
ApoI RAATTY 1 cut(s) 7
AspLEI GCGC 1 cut(s) 399
AsuHPI GGTGA 1 cut(s) 85
BbsI GAAGAC 1 cut(s) 364
BbvI GCAGC 2 cut(s) 331, 359
BccI CCATC 1 cut(s) 23
BciT130I CCWGG 1 cut(s) 123
BfaI CTAG 3 cut(s) 62, 368, 430
BfmI CTRYAG 1 cut(s) 345
BfoI RGCGCY 1 cut(s) 400
BisI GCNGC 2 cut(s) 345, 348
BlsI GCNGC 2 cut(s) 346, 349
Bme1390I CCNGG 1 cut(s) 123
BmiI GGNNCC 1 cut(s) 204
BmrFI CCNGG 1 cut(s) 123
BoxI GACNNNNGTC 1 cut(s) 411
BpiI GAAGAC 1 cut(s) 364
BpuEI CTTGAG 2 cut(s) 261, 360
BsaJI CCNNGG 2 cut(s) 103, 388
BsaXI ACNNNNNCTCC 4 cut(s) 289, 295, 319, 325
Bsc4I CCNNNNNNNGG 1 cut(s) 269
Bse118I RCCGGY 1 cut(s) 136
Bse3DI GCAATG 1 cut(s) 339
BseBI CCWGG 1 cut(s) 123
BseDI CCNNGG 2 cut(s) 103, 388
BseLI CCNNNNNNNGG 1 cut(s) 269
BseMI GCAATG 1 cut(s) 339
BseRI GAGGAG 1 cut(s) 278
BseXI GCAGC 2 cut(s) 331, 359
Bsh1236I CGCG 1 cut(s) 68
BshFI GGCC 1 cut(s) 282
BsiSI CCGG 1 cut(s) 137
BslFI GGGAC 1 cut(s) 201
BslI CCNNNNNNNGG 1 cut(s) 269
BsmFI GGGAC 1 cut(s) 201
BsnI GGCC 1 cut(s) 282
Bsp143I GATC 1 cut(s) 249
BspACI CCGC 2 cut(s) 263, 300
BspANI GGCC 1 cut(s) 282
BspFNI CGCG 1 cut(s) 68
BspLI GGNNCC 1 cut(s) 204
BspMAI CTGCAG 1 cut(s) 349
BsrDI GCAATG 1 cut(s) 339
BsrFI RCCGGY 1 cut(s) 136
BssAI RCCGGY 1 cut(s) 136
BssECI CCNNGG 2 cut(s) 103, 388
BssMI GATC 1 cut(s) 249
BssT1I CCWWGG 2 cut(s) 103, 388
Bst2UI CCWGG 1 cut(s) 123
Bst4CI ACNGT 1 cut(s) 21
BstC8I GCNNGC 3 cut(s) 138, 240, 401
BstDEI CTNAG 1 cut(s) 246
BstFNI CGCG 1 cut(s) 68
BstH2I RGCGCY 1 cut(s) 400
BstHHI GCGC 1 cut(s) 399
BstKTI GATC 1 cut(s) 252
BstMBI GATC 1 cut(s) 249
BstNI CCWGG 1 cut(s) 123
BstPAI GACNNNNGTC 1 cut(s) 411
BstSCI CCNGG 1 cut(s) 121
BstSFI CTRYAG 1 cut(s) 345
BstUI CGCG 1 cut(s) 68
BstV1I GCAGC 2 cut(s) 331, 359
BstV2I GAAGAC 1 cut(s) 364
BsuRI GGCC 1 cut(s) 282
Cac8I GCNNGC 3 cut(s) 138, 240, 401
CfoI GCGC 1 cut(s) 399
Cfr10I RCCGGY 1 cut(s) 136
CseI GACGC 1 cut(s) 74
Csp6I GTAC 1 cut(s) 163
CviAII CATG 2 cut(s) 404, 419
CviJI RGCY 5 cut(s) 205, 211, 259, 282, 350
CviKI_1 RGCY 5 cut(s) 205, 211, 259, 282, 350
CviQI GTAC 1 cut(s) 163
DdeI CTNAG 1 cut(s) 246
DpnI GATC 1 cut(s) 251
DpnII GATC 1 cut(s) 249
EciI GGCGGA 1 cut(s) 252
Eco130I CCWWGG 2 cut(s) 103, 388
Eco32I GATATC 1 cut(s) 146
Eco47III AGCGCT 1 cut(s) 398
Eco57I CTGAAG 1 cut(s) 336
EcoRII CCWGG 1 cut(s) 121
EcoRV GATATC 1 cut(s) 146
EcoT14I CCWWGG 2 cut(s) 103, 388
EcoT22I ATGCAT 1 cut(s) 232
ErhI CCWWGG 2 cut(s) 103, 388
FaeI CATG 2 cut(s) 407, 422
FaiI YATR 4 cut(s) 150, 228, 405, 420
FaqI GGGAC 1 cut(s) 201
FatI CATG 2 cut(s) 403, 418
FblI GTMKAC 1 cut(s) 415
Fnu4HI GCNGC 2 cut(s) 345, 348
Fsp4HI GCNGC 2 cut(s) 345, 348
FspBI CTAG 3 cut(s) 62, 368, 430
GlaI GCGC 1 cut(s) 398
GluI GCNGC 2 cut(s) 345, 348
HaeII RGCGCY 1 cut(s) 400
HaeIII GGCC 1 cut(s) 282
HapII CCGG 1 cut(s) 137
HgaI GACGC 1 cut(s) 74
HhaI GCGC 1 cut(s) 399
Hin1II CATG 2 cut(s) 407, 422
Hin6I GCGC 1 cut(s) 397
HinP1I GCGC 1 cut(s) 397
HincII GTYRAC 1 cut(s) 416
HindII GTYRAC 1 cut(s) 416
HinfI GANTC 2 cut(s) 33, 323
HpaII CCGG 1 cut(s) 137
HphI GGTGA 1 cut(s) 85
Hpy166II GTNNAC 1 cut(s) 416
Hpy188I TCNGA 2 cut(s) 202, 254
Hpy188III TCNNGA 4 cut(s) 24, 37, 314, 377
Hpy8I GTNNAC 1 cut(s) 416
HpyCH4III ACNGT 1 cut(s) 21
HpyCH4V TGCA 3 cut(s) 230, 347, 403
HpyF3I CTNAG 1 cut(s) 246
Hsp92II CATG 2 cut(s) 407, 422
HspAI GCGC 1 cut(s) 397
KroI GCCGGC 1 cut(s) 136
KroNI GCCGGC 1 cut(s) 138
Kzo9I GATC 1 cut(s) 249
LmnI GCTCC 1 cut(s) 202
LpnPI CCDG 6 cut(s) 37, 108, 126, 135, 150, 327
Lsp1109I GCAGC 2 cut(s) 331, 359
MaeI CTAG 3 cut(s) 62, 368, 430
MaeIII GTNAC 2 cut(s) 73, 370
MalI GATC 1 cut(s) 251
MboI GATC 1 cut(s) 249
MboII GAAGA 1 cut(s) 369
MluCI AATT 2 cut(s) 7, 108
MluI ACGCGT 1 cut(s) 66
MlyI GAGTC 1 cut(s) 332
MmeI TCCRAC 1 cut(s) 108
MnlI CCTC 4 cut(s) 211, 270, 299, 395
Mph1103I ATGCAT 1 cut(s) 232
MroNI GCCGGC 1 cut(s) 136
MslI CAYNNNNRTG 2 cut(s) 131, 153
MspI CCGG 1 cut(s) 137
MspR9I CCNGG 1 cut(s) 123
MvaI CCWGG 1 cut(s) 123
MvnI CGCG 1 cut(s) 68
NaeI GCCGGC 1 cut(s) 138
NdeII GATC 1 cut(s) 249
NgoMIV GCCGGC 1 cut(s) 136
NlaIII CATG 2 cut(s) 407, 422
NlaIV GGNNCC 1 cut(s) 204
NmuCI GTSAC 2 cut(s) 73, 370
NsiI ATGCAT 1 cut(s) 232
PdiI GCCGGC 1 cut(s) 138
PfeI GAWTC 1 cut(s) 33
PkrI GCNGC 2 cut(s) 346, 349
PleI GAGTC 1 cut(s) 331
PpsI GAGTC 1 cut(s) 331
PshAI GACNNNNGTC 1 cut(s) 411
Psp6I CCWGG 1 cut(s) 121
PspGI CCWGG 1 cut(s) 121
PspN4I GGNNCC 1 cut(s) 204
PstI CTGCAG 1 cut(s) 349
RsaI GTAC 1 cut(s) 164
RsaNI GTAC 1 cut(s) 163
RseI CAYNNNNRTG 2 cut(s) 131, 153
SalI GTCGAC 1 cut(s) 414
SatI GCNGC 2 cut(s) 345, 348
Sau3AI GATC 1 cut(s) 249
SchI GAGTC 1 cut(s) 332
ScrFI CCNGG 1 cut(s) 123
SetI ASST 4 cut(s) 63, 213, 222, 387
SfcI CTRYAG 1 cut(s) 345
SmiMI CAYNNNNRTG 2 cut(s) 131, 153
SmlI CTYRAG 2 cut(s) 276, 375
SmoI CTYRAG 2 cut(s) 276, 375
Sse9I AATT 2 cut(s) 7, 108
SsiI CCGC 2 cut(s) 263, 300
SspMI CTAG 3 cut(s) 62, 368, 430
StyD4I CCNGG 1 cut(s) 121
StyI CCWWGG 2 cut(s) 103, 388
TaaI ACNGT 1 cut(s) 21
TaqI TCGA 2 cut(s) 36, 415
TasI AATT 2 cut(s) 7, 108
TfiI GAWTC 1 cut(s) 33
TseFI GTSAC 2 cut(s) 73, 370
TseI GCWGC 2 cut(s) 344, 347
Tsp45I GTSAC 2 cut(s) 73, 370
TspDTI ATGAA 1 cut(s) 62
XapI RAATTY 1 cut(s) 7
XmiI GTMKAC 1 cut(s) 415
XspI CTAG 3 cut(s) 62, 368, 430
Zsp2I ATGCAT 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.