pycom10g17970

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0024671
Physical Location & Seq
Forward (+)
21126452 .. 21128787
2336 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 843 bp
ATGGTTCACTTAATAATAGTACTTGGCAATTTCCGGAGAAGTTGCGTCCCCCAAAATCCCCAACAACAATGGAGATTAGTTCGCCCACTTCCGGAGGCCGCACTGAACCGCTTCTGGTGTCACAAGACGACGCCGTACAGCAGCTCGGAAACACATCGTGGAGACTCGATGTTAAGGAGTTCCGGCTGCCCCAGCAGAACGGTACTAGCGGTAACGACCATGACAGTGGACGGAGTCGTTTCGCTTTCCGTCGCCTCTGCGCCACCAGAATTTTTCTTGCTTTGGCTTTTAAACTCAGAGAAAAAGTACAAGGTTGAAGAATATTACAAGAAACAAGAAAGGCTTCTTGAAGGGTTCAGTGAGATGGAGACCATGACTGAAGAGGGTTGGTTGCCCGGAAGTCTTACAGAGGATCAAATGAAGCAGCTAGCGAAGAGTGAGCGGATGGCGGTTCATGCATCAAACGTAGCTAATTTGGTTCTTTTTGCTGCAAAAGTTTATGCTTCAATTATGAGCAGATCATTGGCAGTTATTGCCTCTACCTTGGATTCCCTCTTGGATCTCTTATCCGGCTTTATTTTGTGGTTCACTGCTAATGCCATGAAAAATCCCAACCAGTTTCGCTATCCTATTGGGAAGAAACGTATGCAACCAGTGGGTATCATTGTCTTCGCATCTGTTATGGCTACTCTTGGACTGCAAATATTGATCGAGTCTGGTCGGGAACTCGTCTCTCAGGCAGTCACCGCCCGAAAAAATGAATGGCACGCAGGAGAATTGGATGATTGGCATCATGGTCTCGGTCACAGTAGTGAAGTTTGTGCTCATGGTCTACTGTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux ZT_dimer

Protein Analysis

281

Amino Acids

31.34

Weight (kDa)

8.32

Isoelectric Point (pI)

54.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 152 - 244 1e-18 Cation efflux transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024671)

Species Orthologous Gene IDs
pyrus_communis pycom10g17970
rosa_chinensis RchiOBHm_Chr4g0386741
rosa_samantha Rh5AG327700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 442
AccI GTMKAC 1 cut(s) 832
AccIII TCCGGA 2 cut(s) 33, 91
AciI CCGC 6 cut(s) 99, 109, 209, 442, 449, 747
AclWI GGATC 2 cut(s) 420, 567
AcsI RAATTY 1 cut(s) 269
AcuI CTGAAG 1 cut(s) 399
AcyI GRCGYC 1 cut(s) 131
AdeI CACNNNGTG 1 cut(s) 158
AfaI GTAC 4 cut(s) 21, 137, 204, 308
AfiI CCNNNNNNNGG 1 cut(s) 91
AgsI TTSAA 3 cut(s) 317, 350, 507
AleI CACNNNNGTG 1 cut(s) 810
AluBI AGCT 3 cut(s) 144, 427, 470
AluI AGCT 3 cut(s) 144, 427, 470
Alw21I GWGCWC 1 cut(s) 826
Alw26I GTCTC 4 cut(s) 156, 362, 736, 803
AlwI GGATC 2 cut(s) 420, 567
Aor13HI TCCGGA 2 cut(s) 33, 91
AoxI GGCC 1 cut(s) 96
ApeKI GCWGC 4 cut(s) 141, 186, 424, 488
ApoI RAATTY 1 cut(s) 269
Asp700I GAANNNNTTC 2 cut(s) 110, 342
AspLEI GCGC 1 cut(s) 262
AsuC2I CCSGG 1 cut(s) 396
AsuHPI GGTGA 1 cut(s) 736
AsuNHI GCTAGC 1 cut(s) 427
BbsI GAAGAC 1 cut(s) 661
Bbv12I GWGCWC 1 cut(s) 826
BbvI GCAGC 4 cut(s) 153, 173, 436, 475
BccI CCATC 2 cut(s) 358, 439
BceAI ACGGC 1 cut(s) 118
BcnI CCSGG 1 cut(s) 396
BcoDI GTCTC 4 cut(s) 156, 362, 736, 803
BfaI CTAG 2 cut(s) 206, 428
BisI GCNGC 5 cut(s) 99, 142, 187, 425, 489
BlsI GCNGC 5 cut(s) 100, 143, 188, 426, 490
BmcAI AGTACT 1 cut(s) 21
Bme1390I CCNGG 1 cut(s) 396
BmrFI CCNGG 1 cut(s) 396
BmsI GCATC 3 cut(s) 467, 683, 799
BmtI GCTAGC 1 cut(s) 431
BpiI GAAGAC 1 cut(s) 661
BpuMI CCSGG 1 cut(s) 396
BsaBI GATNNNNATC 1 cut(s) 789
BsaHI GRCGYC 1 cut(s) 131
BsaI GGTCTC 2 cut(s) 362, 803
BsaJI CCNNGG 1 cut(s) 543
BsaWI WCCGGW 2 cut(s) 33, 91
Bsc4I CCNNNNNNNGG 1 cut(s) 91
Bse1I ACTGG 2 cut(s) 616, 653
Bse8I GATNNNNATC 1 cut(s) 789
BseAI TCCGGA 2 cut(s) 33, 91
BseDI CCNNGG 1 cut(s) 543
BseGI GGATG 2 cut(s) 450, 787
BseJI GATNNNNATC 1 cut(s) 789
BseLI CCNNNNNNNGG 1 cut(s) 91
BseMII CTCAG 2 cut(s) 309, 749
BseNI ACTGG 2 cut(s) 616, 653
BseXI GCAGC 4 cut(s) 153, 173, 436, 475
BseYI CCCAGC 1 cut(s) 191
BshFI GGCC 1 cut(s) 98
BsiHKAI GWGCWC 1 cut(s) 826
BsiSI CCGG 5 cut(s) 34, 92, 183, 396, 570
BslFI GGGAC 1 cut(s) 32
BslI CCNNNNNNNGG 1 cut(s) 91
BsmAI GTCTC 4 cut(s) 156, 362, 736, 803
BsmBI CGTCTC 1 cut(s) 736
BsmFI GGGAC 1 cut(s) 32
BsnI GGCC 1 cut(s) 98
Bso31I GGTCTC 2 cut(s) 362, 803
Bsp1286I GDGCHC 1 cut(s) 826
Bsp13I TCCGGA 2 cut(s) 33, 91
Bsp143I GATC 4 cut(s) 412, 518, 559, 708
BspACI CCGC 6 cut(s) 99, 109, 209, 442, 449, 747
BspANI GGCC 1 cut(s) 98
BspCNI CTCAG 2 cut(s) 308, 748
BspEI TCCGGA 2 cut(s) 33, 91
BspOI GCTAGC 1 cut(s) 431
BspPI GGATC 2 cut(s) 420, 567
BspTNI GGTCTC 2 cut(s) 362, 803
BsrBI CCGCTC 1 cut(s) 442
BsrI ACTGG 2 cut(s) 616, 653
BssECI CCNNGG 1 cut(s) 543
BssMI GATC 4 cut(s) 412, 518, 559, 708
BssNI GRCGYC 1 cut(s) 131
BssT1I CCWWGG 1 cut(s) 543
Bst4CI ACNGT 4 cut(s) 202, 226, 809, 837
Bst6I CTCTTC 2 cut(s) 375, 428
BstACI GRCGYC 1 cut(s) 131
BstAPI GCANNNNNTGC 1 cut(s) 533
BstC8I GCNNGC 2 cut(s) 429, 768
BstDEI CTNAG 2 cut(s) 295, 735
BstF5I GGATG 2 cut(s) 450, 787
BstHHI GCGC 1 cut(s) 262
BstKTI GATC 4 cut(s) 415, 521, 562, 711
BstMAI GTCTC 4 cut(s) 156, 362, 736, 803
BstMBI GATC 4 cut(s) 412, 518, 559, 708
BstMWI GCNNNNNNNGC 4 cut(s) 192, 455, 533, 746
BstSCI CCNGG 1 cut(s) 394
BstV1I GCAGC 4 cut(s) 153, 173, 436, 475
BstV2I GAAGAC 1 cut(s) 661
BstX2I RGATCY 1 cut(s) 559
BstXI CCANNNNNNTGG 1 cut(s) 226
BstYI RGATCY 1 cut(s) 559
BsuRI GGCC 1 cut(s) 98
BtsCI GGATG 2 cut(s) 450, 787
BtsI GCAGTG 1 cut(s) 588
BtsIMutI CAGTG 5 cut(s) 101, 231, 364, 588, 660
Cac8I GCNNGC 2 cut(s) 429, 768
CfoI GCGC 1 cut(s) 262
CseI GACGC 2 cut(s) 34, 139
Csp6I GTAC 4 cut(s) 20, 136, 203, 307
CviAII CATG 6 cut(s) 220, 373, 455, 601, 794, 827
CviJI RGCY 9 cut(s) 98, 144, 186, 286, 343, 427, 470, 573, 686
CviKI_1 RGCY 9 cut(s) 98, 144, 186, 286, 343, 427, 470, 573, 686
CviQI GTAC 4 cut(s) 20, 136, 203, 307
DdeI CTNAG 2 cut(s) 295, 735
DpnI GATC 4 cut(s) 414, 520, 561, 710
DpnII GATC 4 cut(s) 412, 518, 559, 708
DraI TTTAAA 1 cut(s) 291
DraIII CACNNNGTG 1 cut(s) 158
Eam1104I CTCTTC 2 cut(s) 375, 428
EarI CTCTTC 2 cut(s) 375, 428
Eco130I CCWWGG 1 cut(s) 543
Eco31I GGTCTC 2 cut(s) 362, 803
Eco57I CTGAAG 1 cut(s) 399
EcoT14I CCWWGG 1 cut(s) 543
EcoT22I ATGCAT 1 cut(s) 460
ErhI CCWWGG 1 cut(s) 543
Esp3I CGTCTC 1 cut(s) 736
FaeI CATG 6 cut(s) 223, 376, 458, 604, 797, 830
FalI AAGNNNNNCTT 2 cut(s) 327, 359
FaqI GGGAC 1 cut(s) 32
FatI CATG 6 cut(s) 219, 372, 454, 600, 793, 826
FblI GTMKAC 1 cut(s) 832
Fnu4HI GCNGC 5 cut(s) 99, 142, 187, 425, 489
FokI GGATG 2 cut(s) 457, 794
Fsp4HI GCNGC 5 cut(s) 99, 142, 187, 425, 489
FspBI CTAG 2 cut(s) 206, 428
GlaI GCGC 1 cut(s) 261
GluI GCNGC 5 cut(s) 99, 142, 187, 425, 489
GsaI CCCAGC 1 cut(s) 195
HaeIII GGCC 1 cut(s) 98
HapII CCGG 5 cut(s) 34, 92, 183, 396, 570
HgaI GACGC 2 cut(s) 34, 139
HhaI GCGC 1 cut(s) 262
Hin1I GRCGYC 1 cut(s) 131
Hin1II CATG 6 cut(s) 223, 376, 458, 604, 797, 830
Hin6I GCGC 1 cut(s) 260
HinP1I GCGC 1 cut(s) 260
HinfI GANTC 4 cut(s) 164, 234, 548, 713
HpaII CCGG 5 cut(s) 34, 92, 183, 396, 570
HphI GGTGA 1 cut(s) 736
Hpy166II GTNNAC 4 cut(s) 7, 229, 588, 833
Hpy188I TCNGA 2 cut(s) 148, 298
Hpy188III TCNNGA 4 cut(s) 34, 92, 347, 722
Hpy8I GTNNAC 4 cut(s) 7, 229, 588, 833
Hpy99I CGWCG 2 cut(s) 133, 254
HpyAV CCTTC 1 cut(s) 344
HpyCH4III ACNGT 4 cut(s) 202, 226, 809, 837
HpyCH4IV ACGT 2 cut(s) 465, 643
HpyCH4V TGCA 4 cut(s) 458, 491, 649, 700
HpyF10VI GCNNNNNNNGC 4 cut(s) 192, 455, 533, 746
HpyF3I CTNAG 2 cut(s) 295, 735
HpySE526I ACGT 2 cut(s) 465, 643
Hsp92I GRCGYC 1 cut(s) 131
Hsp92II CATG 6 cut(s) 223, 376, 458, 604, 797, 830
HspAI GCGC 1 cut(s) 260
Kpn2I TCCGGA 2 cut(s) 33, 91
Kzo9I GATC 4 cut(s) 412, 518, 559, 708
Lsp1109I GCAGC 4 cut(s) 153, 173, 436, 475
LweI GCATC 3 cut(s) 467, 683, 799
MaeI CTAG 2 cut(s) 206, 428
MaeII ACGT 2 cut(s) 465, 643
MaeIII GTNAC 4 cut(s) 119, 211, 742, 803
MalI GATC 4 cut(s) 414, 520, 561, 710
MbiI CCGCTC 1 cut(s) 442
MboI GATC 4 cut(s) 412, 518, 559, 708
MboII GAAGA 5 cut(s) 329, 392, 445, 649, 661
MflI RGATCY 1 cut(s) 559
MhlI GDGCHC 1 cut(s) 826
MluCI AATT 5 cut(s) 28, 269, 472, 507, 776
MlyI GAGTC 3 cut(s) 158, 243, 722
MnlI CCTC 6 cut(s) 88, 265, 376, 403, 547, 563
Mph1103I ATGCAT 1 cut(s) 460
MroI TCCGGA 2 cut(s) 33, 91
MroXI GAANNNNTTC 2 cut(s) 110, 342
MseI TTAA 3 cut(s) 11, 173, 290
MslI CAYNNNNRTG 2 cut(s) 224, 810
MspI CCGG 5 cut(s) 34, 92, 183, 396, 570
MspR9I CCNGG 1 cut(s) 396
MwoI GCNNNNNNNGC 4 cut(s) 192, 455, 533, 746
NciI CCSGG 1 cut(s) 396
NdeII GATC 4 cut(s) 412, 518, 559, 708
NheI GCTAGC 1 cut(s) 427
NlaIII CATG 6 cut(s) 223, 376, 458, 604, 797, 830
NmuCI GTSAC 3 cut(s) 119, 742, 803
NsiI ATGCAT 1 cut(s) 460
OliI CACNNNNGTG 1 cut(s) 810
PdmI GAANNNNTTC 2 cut(s) 110, 342
PfeI GAWTC 1 cut(s) 548
PflFI GACNNNGTC 1 cut(s) 233
PkrI GCNGC 5 cut(s) 100, 143, 188, 426, 490
PleI GAGTC 3 cut(s) 158, 242, 721
PpsI GAGTC 3 cut(s) 158, 242, 721
PspFI CCCAGC 1 cut(s) 191
PsuI RGATCY 1 cut(s) 559
PsyI GACNNNGTC 1 cut(s) 233
RsaI GTAC 4 cut(s) 21, 137, 204, 308
RsaNI GTAC 4 cut(s) 20, 136, 203, 307
RseI CAYNNNNRTG 2 cut(s) 224, 810
SaqAI TTAA 3 cut(s) 11, 173, 290
SatI GCNGC 5 cut(s) 99, 142, 187, 425, 489
Sau3AI GATC 4 cut(s) 412, 518, 559, 708
ScaI AGTACT 1 cut(s) 21
SchI GAGTC 3 cut(s) 158, 243, 722
ScrFI CCNGG 1 cut(s) 396
SduI GDGCHC 1 cut(s) 826
SetI ASST 7 cut(s) 146, 315, 429, 468, 472, 545, 646
SfaNI GCATC 3 cut(s) 467, 683, 799
SmiMI CAYNNNNRTG 2 cut(s) 224, 810
Sse9I AATT 5 cut(s) 28, 269, 472, 507, 776
SsiI CCGC 6 cut(s) 99, 109, 209, 442, 449, 747
SspI AATATT 2 cut(s) 323, 705
SspMI CTAG 2 cut(s) 206, 428
StyD4I CCNGG 1 cut(s) 394
StyI CCWWGG 1 cut(s) 543
TaaI ACNGT 4 cut(s) 202, 226, 809, 837
TaiI ACGT 2 cut(s) 468, 646
TaqI TCGA 2 cut(s) 167, 711
TaqII GACCGA 1 cut(s) 791
TasI AATT 5 cut(s) 28, 269, 472, 507, 776
TatI WGTACW 2 cut(s) 19, 306
TauI GCSGC 1 cut(s) 101
TfiI GAWTC 1 cut(s) 548
Tru1I TTAA 3 cut(s) 11, 173, 290
Tru9I TTAA 3 cut(s) 11, 173, 290
TscAI CASTG 5 cut(s) 108, 231, 364, 595, 660
TseFI GTSAC 3 cut(s) 119, 742, 803
TseI GCWGC 4 cut(s) 141, 186, 424, 488
Tsp45I GTSAC 3 cut(s) 119, 742, 803
TspDTI ATGAA 4 cut(s) 434, 443, 617, 774
TspGWI ACGGA 2 cut(s) 238, 246
TspRI CASTG 5 cut(s) 108, 231, 364, 595, 660
Tth111I GACNNNGTC 1 cut(s) 233
XapI RAATTY 1 cut(s) 269
XmiI GTMKAC 1 cut(s) 832
XmnI GAANNNNTTC 2 cut(s) 110, 342
XspI CTAG 2 cut(s) 206, 428
ZrmI AGTACT 1 cut(s) 21
Zsp2I ATGCAT 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.