RchiOBHm_Chr4g0386741

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0024671
Physical Location & Seq
Reverse (-)
2157212 .. 2162974
5763 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36009

Sequence Viewer

Length: 360 bp
ATGAAGCAGCTAGCTAAGAGTGAGAGGATGGCAATTCATATATCAAATGTAGCAAATTTGCTGCTCTTTTCTGCAAAAGTTTATGCTTCAATCATGAGTAGATCACTGGCAGTTATTGCCTCTACCTTGGATTCCCCCTTGGATCTCATGTCTGGCTTTATTTTGTGGTTCACTGCCCATGCCATGAGAAAGCCAAACCGCTTTCATTATCCTATTGGAAAGAAACGTATGCAACCAGCGGGTATCATTGTCTTTGCATCTGTTATGGCAACTCTTGGACTGCAAATATTGCTTGAGTCTGCTCGGGAACTCGTCTCAAAGGTGTTGAGGGAGCTACTTGAGCTCGTTTGTTTTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux ZT_dimer

Protein Analysis

119

Amino Acids

13.29

Weight (kDa)

10.09

Isoelectric Point (pI)

55.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 13 - 105 6.7e-17 Cation efflux transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0024671)

Species Orthologous Gene IDs
pyrus_communis pycom10g17970
rosa_chinensis RchiOBHm_Chr4g0386741
rosa_samantha Rh5AG327700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 199, 239
AclWI GGATC 1 cut(s) 150
AcsI RAATTY 1 cut(s) 55
AgsI TTSAA 1 cut(s) 90
AluBI AGCT 4 cut(s) 10, 14, 334, 343
AluI AGCT 4 cut(s) 10, 14, 334, 343
Alw21I GWGCWC 1 cut(s) 345
Alw26I GTCTC 1 cut(s) 319
AlwI GGATC 1 cut(s) 150
Ama87I CYCGRG 1 cut(s) 303
ApeKI GCWGC 2 cut(s) 7, 61
ApoI RAATTY 1 cut(s) 55
AsuNHI GCTAGC 1 cut(s) 10
AvaI CYCGRG 1 cut(s) 303
BanII GRGCYC 1 cut(s) 345
Bbv12I GWGCWC 1 cut(s) 345
BbvI GCAGC 2 cut(s) 19, 48
BccI CCATC 1 cut(s) 22
BcoDI GTCTC 1 cut(s) 319
BfaI CTAG 1 cut(s) 11
BisI GCNGC 2 cut(s) 8, 62
BlsI GCNGC 2 cut(s) 9, 63
BmeT110I CYCGRG 1 cut(s) 303
BmsI GCATC 1 cut(s) 266
BmtI GCTAGC 1 cut(s) 14
BpuEI CTTGAG 2 cut(s) 314, 359
BsaJI CCNNGG 2 cut(s) 126, 138
Bse1I ACTGG 1 cut(s) 111
BseDI CCNNGG 2 cut(s) 126, 138
BseGI GGATG 1 cut(s) 33
BseNI ACTGG 1 cut(s) 111
BseXI GCAGC 2 cut(s) 19, 48
BsiHKAI GWGCWC 1 cut(s) 345
BsiHKCI CYCGRG 1 cut(s) 303
BsmAI GTCTC 1 cut(s) 319
BsmBI CGTCTC 1 cut(s) 319
BsoBI CYCGRG 1 cut(s) 303
Bsp1286I GDGCHC 1 cut(s) 345
Bsp143I GATC 2 cut(s) 101, 142
BspACI CCGC 2 cut(s) 199, 239
BspHI TCATGA 1 cut(s) 93
BspOI GCTAGC 1 cut(s) 14
BspPI GGATC 1 cut(s) 150
BsrI ACTGG 1 cut(s) 111
BssECI CCNNGG 2 cut(s) 126, 138
BssMI GATC 2 cut(s) 101, 142
BssT1I CCWWGG 2 cut(s) 126, 138
BstAPI GCANNNNNTGC 2 cut(s) 116, 289
BstC8I GCNNGC 1 cut(s) 12
BstDEI CTNAG 1 cut(s) 15
BstF5I GGATG 1 cut(s) 33
BstKTI GATC 2 cut(s) 104, 145
BstMAI GTCTC 1 cut(s) 319
BstMBI GATC 2 cut(s) 101, 142
BstMWI GCNNNNNNNGC 3 cut(s) 116, 289, 340
BstV1I GCAGC 2 cut(s) 19, 48
BstX2I RGATCY 1 cut(s) 142
BstYI RGATCY 1 cut(s) 142
BtsCI GGATG 1 cut(s) 33
BtsI GCAGTG 1 cut(s) 171
BtsIMutI CAGTG 2 cut(s) 104, 171
Cac8I GCNNGC 1 cut(s) 12
CciI TCATGA 1 cut(s) 93
CviAII CATG 4 cut(s) 94, 148, 179, 184
CviJI RGCY 6 cut(s) 10, 14, 156, 193, 334, 343
CviKI_1 RGCY 6 cut(s) 10, 14, 156, 193, 334, 343
DdeI CTNAG 1 cut(s) 15
DpnI GATC 2 cut(s) 103, 144
DpnII GATC 2 cut(s) 101, 142
Ecl136II GAGCTC 1 cut(s) 343
Eco130I CCWWGG 2 cut(s) 126, 138
Eco24I GRGCYC 1 cut(s) 345
Eco53kI GAGCTC 1 cut(s) 343
Eco88I CYCGRG 1 cut(s) 303
EcoICRI GAGCTC 1 cut(s) 343
EcoT14I CCWWGG 2 cut(s) 126, 138
EcoT38I GRGCYC 1 cut(s) 345
ErhI CCWWGG 2 cut(s) 126, 138
Esp3I CGTCTC 1 cut(s) 319
FaeI CATG 4 cut(s) 97, 151, 182, 187
FaiI YATR 9 cut(s) 39, 41, 84, 95, 149, 180, 185, 230, 266
FatI CATG 4 cut(s) 93, 147, 178, 183
FauI CCCGC 1 cut(s) 232
Fnu4HI GCNGC 2 cut(s) 8, 62
FokI GGATG 1 cut(s) 40
FriOI GRGCYC 1 cut(s) 345
Fsp4HI GCNGC 2 cut(s) 8, 62
FspBI CTAG 1 cut(s) 11
GluI GCNGC 2 cut(s) 8, 62
Hin1II CATG 4 cut(s) 97, 151, 182, 187
HinfI GANTC 2 cut(s) 131, 296
Hpy166II GTNNAC 1 cut(s) 171
Hpy188III TCNNGA 2 cut(s) 94, 305
Hpy8I GTNNAC 1 cut(s) 171
HpyCH4IV ACGT 1 cut(s) 226
HpyCH4V TGCA 4 cut(s) 74, 232, 257, 283
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 289, 340
HpyF3I CTNAG 1 cut(s) 15
HpySE526I ACGT 1 cut(s) 226
Hsp92II CATG 4 cut(s) 97, 151, 182, 187
Kzo9I GATC 2 cut(s) 101, 142
LmnI GCTCC 1 cut(s) 331
LpnPI CCDG 3 cut(s) 92, 138, 249
Lsp1109I GCAGC 2 cut(s) 19, 48
LweI GCATC 1 cut(s) 266
MaeI CTAG 1 cut(s) 11
MaeII ACGT 1 cut(s) 226
MalI GATC 2 cut(s) 103, 144
MboI GATC 2 cut(s) 101, 142
MflI RGATCY 1 cut(s) 142
MhlI GDGCHC 1 cut(s) 345
MluCI AATT 2 cut(s) 33, 55
MlyI GAGTC 1 cut(s) 305
MnlI CCTC 3 cut(s) 18, 130, 321
MspA1I CMGCKG 1 cut(s) 239
MwoI GCNNNNNNNGC 3 cut(s) 116, 289, 340
NdeII GATC 2 cut(s) 101, 142
NheI GCTAGC 1 cut(s) 10
NlaIII CATG 4 cut(s) 97, 151, 182, 187
PagI TCATGA 1 cut(s) 93
PfeI GAWTC 1 cut(s) 131
PkrI GCNGC 2 cut(s) 9, 63
PleI GAGTC 1 cut(s) 304
PpsI GAGTC 1 cut(s) 304
Psp124BI GAGCTC 1 cut(s) 345
PsuI RGATCY 1 cut(s) 142
SacI GAGCTC 1 cut(s) 345
SatI GCNGC 2 cut(s) 8, 62
Sau3AI GATC 2 cut(s) 101, 142
SchI GAGTC 1 cut(s) 305
SduI GDGCHC 1 cut(s) 345
SetI ASST 7 cut(s) 12, 16, 128, 229, 324, 336, 345
SfaNI GCATC 1 cut(s) 266
SmlI CTYRAG 2 cut(s) 293, 338
SmoI CTYRAG 2 cut(s) 293, 338
Sse9I AATT 2 cut(s) 33, 55
SsiI CCGC 2 cut(s) 199, 239
SspI AATATT 1 cut(s) 288
SspMI CTAG 1 cut(s) 11
SstI GAGCTC 1 cut(s) 345
StyI CCWWGG 2 cut(s) 126, 138
TaiI ACGT 1 cut(s) 229
TasI AATT 2 cut(s) 33, 55
TfiI GAWTC 1 cut(s) 131
TscAI CASTG 2 cut(s) 111, 178
TseI GCWGC 2 cut(s) 7, 61
TspDTI ATGAA 3 cut(s) 17, 26, 194
TspRI CASTG 2 cut(s) 111, 178
XapI RAATTY 1 cut(s) 55
XspI CTAG 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.