pycom10g20650

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0028220
Physical Location & Seq
Forward (+)
23488586 .. 23490481
1896 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1896 bp
ATGGGATTATTACAAACGAGTAAGTTTACATTACCCATTCAAACAAGGACTTTTGCACCAGTATATAATATATGCCCTCCCCATGAACGAATCACAAACAAACAGAAGAGAACAATTATACTTTTGAGAGCTCTAACCCGTACAAACCTTGTTGCAAGCCTCCAACACAGATTTTTGGCGTTAGATTTCGGAGACATGGGTGGTGCTGCCTCCACATCCAAGGTAACGGGTGTTAGCGAACAAAACGATCCGCTATGGCTTTGTGGACAAAGAAAAGACGCGCTGGAATTGGCCATCAAGAGAAGGTCCCTGTTTGTGGATGCATTATGCAAGTATAACCGATCGCTTTACGAAGTAGCAATCGCTCTAAGGATTGCAAACGTCCATTCTCAAACCAAAGATTGCTCGTCTTCAGATTCCATAATTTCGATGAGCTCCGCAAAGGTAAATAGAGAAAAAGTAGGACAAGAAACCCAACATCTCAAAGAAGAAGAAGGCAACGTTGAGACTCAAAGGCGTTCTGGTGACGAGAGTAGTGAAAAAAGAGAGGGAAATTTGAGTGATTCTGATGTGGGTCAAGGGAGGGAATTGGTGGAAGCATTGAAAGATGTTGAGGACTATTTCATTAAAGCCTATGAATCTGGTTTGGAGGTTTCTAAGATGCTTGATGCCAATATGGATCAAATACAATCTTCTGTGGAGGAAATTGAAGAAAAATCGAACAAGTTCAAGCGTTCATTGACGACACGAAACCACCCAAACAAGCTCATGCGGTCACTGCCACTAAAACGCTCCACCTCGTCTAGATGTTCCTCCAAAAGCGTCCACAAATCAAGCTCCAAAGCTTCTTCAAAATATTATTTCAACAGTGGTATTTCTGATGGCAATGGAGCAAAGACATTAATGGGCCATTCGTTCACGCTGAGAGAATTACTTGAGCAAGTTAAAATACTTCATAAAACGATGATGGCTACAGAGGAATCCAGGAAAGAATTGAAGGAATTACAAAAGGAAAAGAAAAGAGAAGGTGTGCTCAAGGGTACTAGGGACAGTACAGGGGCTGGAATAGCGAAGTTGGAGGCCAGAATCAAGGTTTTTGAAAAAGAAGAAGAAGCACAGTACTCTGAAATTCATAGGCTAACAGATAAAGAGATGAAGCCACAACTTGTTGCCTTGTTTCACTGCCTAATGAGAAGTATGAGAATCGTGTCGGAATATCATGAGACCCAAACCAGAATCATGAAGACAGTAAAAGTGCCCGACGGAGTGTTCTGCAGTGATTCACACCAGCTTACTACCTACTATAAGCTTGAAGCAGAAATTCCAAAATGGTGTGTTTGCTTTACATCATATTTCTCTTCACTGAAGGCCTATGTTGAAGCTCTTAATAACGGGCTATTCAAGTTTGCTGCTGCTAACAACGACAAGCCTTCGCTATATCATCATTGGTTAGTTTGTTTGAATAAACTTCCATATAAAGATGTGACGTCTGCCATGACAGAGTTTGGGAATGATGTCCGAGAGCTAATGATTCAACAAGGCGAGGAGCATAAGCTAAAGAAAACGGTTGACAAACTTTCTAGAAAAATACGTGAAAACTCAAACAAGGAGCATTTGAAAAAGAAGGTAGATGAAGGGAAGAAGGAGCAGTTGATTGAGGAGAAGAGGCAAGTGGAAAAGAAGCTAAAGGCAGAAAAGTTGAAAACGCGTCATTTCGCTGAGAGTGGATTTCAGGAAGGATTCTCCAAGGTTTTCGAATACCTGGCTAATTATTCAAGGGGTGCTACGGAAATGTATGATGGCCTTATAGCATTCGACGGAGATGCATGCGTGGGGATTGATGACGAAAGTAACAGCAGTTCATCAGAACATGTTACAGGCAATTTCATAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

632

Amino Acids

71.64

Weight (kDa)

8.9

Isoelectric Point (pI)

44.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF630 PF04783 70 - 124 5.2e-08 Protein of unknown function (DUF630)
DUF632 PF04782 197 - 507 6.5e-43 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028220)

Species Orthologous Gene IDs
malus_domestica MD10G1248400.v1.1
pyrus_communis pycom10g20650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1490
AccII CGCG 2 cut(s) 281, 1708
AciI CCGC 3 cut(s) 251, 438, 772
AclI AACGTT 1 cut(s) 501
AclWI GGATC 2 cut(s) 242, 687
AcoI YGGCCR 1 cut(s) 291
AcsI RAATTY 3 cut(s) 553, 1128, 1320
AcuI CTGAAG 2 cut(s) 396, 1385
AcyI GRCGYC 1 cut(s) 1487
AfaI GTAC 4 cut(s) 142, 1042, 1054, 1121
AfiI CCNNNNNNNGG 1 cut(s) 316
AflIII ACRYGT 2 cut(s) 1706, 1870
AjnI CCWGG 2 cut(s) 983, 1761
Alw21I GWGCWC 3 cut(s) 133, 437, 1035
Alw26I GTCTC 3 cut(s) 186, 500, 1217
AlwI GGATC 2 cut(s) 242, 687
AlwNI CAGNNNCTG 1 cut(s) 1061
AoxI GGCC 5 cut(s) 291, 907, 1080, 1368, 1801
ApeKI GCWGC 3 cut(s) 206, 1409, 1412
ApoI RAATTY 3 cut(s) 553, 1128, 1320
AseI ATTAAT 1 cut(s) 902
Asp700I GAANNNNTTC 1 cut(s) 725
AspLEI GCGC 1 cut(s) 283
AspS9I GGNCC 2 cut(s) 306, 907
AsuHPI GGTGA 1 cut(s) 536
AsuII TTCGAA 1 cut(s) 1755
AvaII GGWCC 1 cut(s) 306
BaeGI GKGCMC 1 cut(s) 1260
BalI TGGCCA 1 cut(s) 293
BanII GRGCYC 2 cut(s) 133, 437
BbsI GAAGAC 2 cut(s) 402, 1250
Bbv12I GWGCWC 3 cut(s) 133, 437, 1035
BbvI GCAGC 3 cut(s) 193, 1396, 1399
BccI CCATC 4 cut(s) 302, 875, 961, 1793
BcgI CGANNNNNNTGC 2 cut(s) 1805, 1839
BciT130I CCWGG 2 cut(s) 985, 1763
BcoDI GTCTC 3 cut(s) 186, 500, 1217
BfaI CTAG 3 cut(s) 804, 1044, 1581
BfmI CTRYAG 2 cut(s) 972, 1273
BisI GCNGC 3 cut(s) 207, 1410, 1413
BlsI GCNGC 3 cut(s) 208, 1411, 1414
BmcAI AGTACT 1 cut(s) 1121
Bme1390I CCNGG 2 cut(s) 985, 1763
Bme18I GGWCC 1 cut(s) 306
BmgT120I GGNCC 2 cut(s) 306, 907
BmiI GGNNCC 1 cut(s) 308
BmrFI CCNGG 2 cut(s) 985, 1763
BmsI GCATC 4 cut(s) 310, 651, 658, 1813
BpiI GAAGAC 2 cut(s) 402, 1250
Bpu14I TTCGAA 1 cut(s) 1755
BpuEI CTTGAG 2 cut(s) 956, 1019
BsaAI YACGTR 1 cut(s) 1592
BsaHI GRCGYC 1 cut(s) 1487
BsaI GGTCTC 1 cut(s) 1217
BsaJI CCNNGG 2 cut(s) 219, 1746
Bsc4I CCNNNNNNNGG 1 cut(s) 316
Bse1I ACTGG 1 cut(s) 59
Bse3DI GCAATG 1 cut(s) 892
BseBI CCWGG 2 cut(s) 985, 1763
BseDI CCNNGG 2 cut(s) 219, 1746
BseGI GGATG 2 cut(s) 215, 325
BseLI CCNNNNNNNGG 1 cut(s) 316
BseMI GCAATG 1 cut(s) 892
BseMII CTCAG 2 cut(s) 914, 1710
BseNI ACTGG 1 cut(s) 59
BseRI GAGGAG 2 cut(s) 1559, 1673
BseSI GKGCMC 1 cut(s) 1260
BseXI GCAGC 3 cut(s) 193, 1396, 1399
Bsh1236I CGCG 2 cut(s) 281, 1708
Bsh1285I CGRYCG 1 cut(s) 344
BshFI GGCC 5 cut(s) 293, 909, 1082, 1370, 1803
BsiEI CGRYCG 1 cut(s) 344
BsiHKAI GWGCWC 3 cut(s) 133, 437, 1035
BslFI GGGAC 2 cut(s) 292, 1061
BslI CCNNNNNNNGG 1 cut(s) 316
BsmAI GTCTC 3 cut(s) 186, 500, 1217
BsmFI GGGAC 2 cut(s) 292, 1061
BsmI GAATGC 1 cut(s) 1811
BsnI GGCC 5 cut(s) 293, 909, 1082, 1370, 1803
Bso31I GGTCTC 1 cut(s) 1217
Bsp119I TTCGAA 1 cut(s) 1755
Bsp1286I GDGCHC 4 cut(s) 133, 437, 1035, 1260
Bsp143I GATC 3 cut(s) 247, 341, 679
BspACI CCGC 3 cut(s) 251, 438, 772
BspANI GGCC 5 cut(s) 293, 909, 1082, 1370, 1803
BspCNI CTCAG 2 cut(s) 915, 1711
BspFNI CGCG 2 cut(s) 281, 1708
BspHI TCATGA 2 cut(s) 1219, 1239
BspLI GGNNCC 1 cut(s) 308
BspMAI CTGCAG 1 cut(s) 1277
BspPI GGATC 2 cut(s) 242, 687
BspT104I TTCGAA 1 cut(s) 1755
BspTNI GGTCTC 1 cut(s) 1217
BsrDI GCAATG 1 cut(s) 892
BsrI ACTGG 1 cut(s) 59
BssECI CCNNGG 2 cut(s) 219, 1746
BssMI GATC 3 cut(s) 247, 341, 679
BssNI GRCGYC 1 cut(s) 1487
BssT1I CCWWGG 2 cut(s) 219, 1746
Bst2UI CCWGG 2 cut(s) 985, 1763
Bst4CI ACNGT 5 cut(s) 869, 1052, 1119, 1249, 1567
Bst6I CTCTTC 3 cut(s) 101, 1363, 1658
BstACI GRCGYC 1 cut(s) 1487
BstBAI YACGTR 1 cut(s) 1592
BstBI TTCGAA 1 cut(s) 1755
BstC8I GCNNGC 2 cut(s) 157, 1828
BstDEI CTNAG 4 cut(s) 368, 657, 923, 1719
BstF5I GGATG 2 cut(s) 215, 325
BstFNI CGCG 2 cut(s) 281, 1708
BstHHI GCGC 1 cut(s) 283
BstKTI GATC 3 cut(s) 250, 344, 682
BstMAI GTCTC 3 cut(s) 186, 500, 1217
BstMBI GATC 3 cut(s) 247, 341, 679
BstMCI CGRYCG 1 cut(s) 344
BstMWI GCNNNNNNNGC 2 cut(s) 778, 1067
BstNI CCWGG 2 cut(s) 985, 1763
BstNSI RCATGY 2 cut(s) 1830, 1874
BstSCI CCNGG 2 cut(s) 983, 1761
BstSFI CTRYAG 2 cut(s) 972, 1273
BstSLI GKGCMC 1 cut(s) 1260
BstUI CGCG 2 cut(s) 281, 1708
BstV1I GCAGC 3 cut(s) 193, 1396, 1399
BstV2I GAAGAC 2 cut(s) 402, 1250
BsuRI GGCC 5 cut(s) 293, 909, 1082, 1370, 1803
BtsCI GGATG 2 cut(s) 215, 325
BtsI GCAGTG 3 cut(s) 776, 1180, 1282
BtsIMutI CAGTG 5 cut(s) 776, 874, 1180, 1282, 1361
Cac8I GCNNGC 2 cut(s) 157, 1828
CaiI CAGNNNCTG 1 cut(s) 1061
CciI TCATGA 2 cut(s) 1219, 1239
CfoI GCGC 1 cut(s) 283
Cfr13I GGNCC 2 cut(s) 306, 907
CseI GACGC 3 cut(s) 287, 811, 1697
Csp6I GTAC 4 cut(s) 141, 1041, 1053, 1120
CviAII CATG 8 cut(s) 83, 196, 769, 1220, 1240, 1495, 1827, 1871
CviQI GTAC 4 cut(s) 141, 1041, 1053, 1120
DdeI CTNAG 4 cut(s) 368, 657, 923, 1719
DpnI GATC 3 cut(s) 249, 343, 681
DpnII GATC 3 cut(s) 247, 341, 679
EaeI YGGCCR 1 cut(s) 291
Eam1104I CTCTTC 3 cut(s) 101, 1363, 1658
EarI CTCTTC 3 cut(s) 101, 1363, 1658
Ecl136II GAGCTC 2 cut(s) 131, 435
Eco130I CCWWGG 2 cut(s) 219, 1746
Eco147I AGGCCT 1 cut(s) 1370
Eco24I GRGCYC 2 cut(s) 133, 437
Eco31I GGTCTC 1 cut(s) 1217
Eco47I GGWCC 1 cut(s) 306
Eco53kI GAGCTC 2 cut(s) 131, 435
Eco57I CTGAAG 2 cut(s) 396, 1385
EcoICRI GAGCTC 2 cut(s) 131, 435
EcoO109I RGGNCCY 1 cut(s) 306
EcoRII CCWGG 2 cut(s) 983, 1761
EcoT14I CCWWGG 2 cut(s) 219, 1746
EcoT22I ATGCAT 2 cut(s) 325, 1828
EcoT38I GRGCYC 2 cut(s) 133, 437
ErhI CCWWGG 2 cut(s) 219, 1746
FaeI CATG 8 cut(s) 86, 199, 772, 1223, 1243, 1498, 1830, 1874
FaqI GGGAC 2 cut(s) 292, 1061
FatI CATG 8 cut(s) 82, 195, 768, 1219, 1239, 1494, 1826, 1870
Fnu4HI GCNGC 3 cut(s) 207, 1410, 1413
FokI GGATG 2 cut(s) 202, 332
FriOI GRGCYC 2 cut(s) 133, 437
Fsp4HI GCNGC 3 cut(s) 207, 1410, 1413
FspBI CTAG 3 cut(s) 804, 1044, 1581
GlaI GCGC 1 cut(s) 282
GluI GCNGC 3 cut(s) 207, 1410, 1413
HaeIII GGCC 5 cut(s) 293, 909, 1082, 1370, 1803
HgaI GACGC 3 cut(s) 287, 811, 1697
HhaI GCGC 1 cut(s) 283
Hin1I GRCGYC 1 cut(s) 1487
Hin1II CATG 8 cut(s) 86, 199, 772, 1223, 1243, 1498, 1830, 1874
Hin6I GCGC 1 cut(s) 281
HinP1I GCGC 1 cut(s) 281
HincII GTYRAC 1 cut(s) 1570
HindII GTYRAC 1 cut(s) 1570
HindIII AAGCTT 2 cut(s) 843, 1307
HphI GGTGA 1 cut(s) 536
Hpy166II GTNNAC 5 cut(s) 27, 266, 826, 918, 1570
Hpy188I TCNGA 8 cut(s) 191, 415, 568, 880, 1126, 1213, 1520, 1867
Hpy188III TCNNGA 6 cut(s) 298, 804, 1220, 1240, 1581, 1733
Hpy8I GTNNAC 5 cut(s) 27, 266, 826, 918, 1570
Hpy99I CGWCG 2 cut(s) 1265, 1820
HpyCH4III ACNGT 5 cut(s) 869, 1052, 1119, 1249, 1567
HpyCH4IV ACGT 4 cut(s) 381, 501, 1487, 1591
HpyCH4V TGCA 7 cut(s) 56, 155, 323, 330, 377, 1275, 1826
HpyF10VI GCNNNNNNNGC 2 cut(s) 778, 1067
HpyF3I CTNAG 4 cut(s) 368, 657, 923, 1719
HpySE526I ACGT 4 cut(s) 381, 501, 1487, 1591
Hsp92I GRCGYC 1 cut(s) 1487
Hsp92II CATG 8 cut(s) 86, 199, 772, 1223, 1243, 1498, 1830, 1874
HspAI GCGC 1 cut(s) 281
Kzo9I GATC 3 cut(s) 247, 341, 679
LmnI GCTCC 7 cut(s) 440, 797, 842, 890, 1546, 1609, 1645
Lsp1109I GCAGC 3 cut(s) 193, 1396, 1399
LweI GCATC 4 cut(s) 310, 651, 658, 1813
MaeI CTAG 3 cut(s) 804, 1044, 1581
MaeII ACGT 4 cut(s) 381, 501, 1487, 1591
MaeIII GTNAC 6 cut(s) 223, 524, 774, 1483, 1850, 1873
MalI GATC 3 cut(s) 249, 343, 681
MboI GATC 3 cut(s) 247, 341, 679
MhlI GDGCHC 4 cut(s) 133, 437, 1035, 1260
MlsI TGGCCA 1 cut(s) 293
MluI ACGCGT 1 cut(s) 1706
MluNI TGGCCA 1 cut(s) 293
MlyI GAGTC 1 cut(s) 502
MmeI TCCRAC 3 cut(s) 187, 1056, 1191
Mox20I TGGCCA 1 cut(s) 293
Mph1103I ATGCAT 2 cut(s) 325, 1828
MroXI GAANNNNTTC 1 cut(s) 725
MscI TGGCCA 1 cut(s) 293
MseI TTAA 4 cut(s) 627, 902, 945, 1386
Msp20I TGGCCA 1 cut(s) 293
MspR9I CCNGG 2 cut(s) 985, 1763
Mva1269I GAATGC 1 cut(s) 1811
MvaI CCWGG 2 cut(s) 985, 1763
MvnI CGCG 2 cut(s) 281, 1708
MwoI GCNNNNNNNGC 2 cut(s) 778, 1067
NdeII GATC 3 cut(s) 247, 341, 679
NlaIII CATG 8 cut(s) 86, 199, 772, 1223, 1243, 1498, 1830, 1874
NlaIV GGNNCC 1 cut(s) 308
NmuCI GTSAC 3 cut(s) 524, 774, 1483
NsiI ATGCAT 2 cut(s) 325, 1828
NspI RCATGY 2 cut(s) 1830, 1874
NspV TTCGAA 1 cut(s) 1755
PaeI GCATGC 1 cut(s) 1830
PagI TCATGA 2 cut(s) 1219, 1239
PceI AGGCCT 1 cut(s) 1370
PciI ACATGT 1 cut(s) 1870
PctI GAATGC 1 cut(s) 1811
PdmI GAANNNNTTC 1 cut(s) 725
PfoI TCCNGGA 1 cut(s) 983
PkrI GCNGC 3 cut(s) 208, 1411, 1414
Ple19I CGATCG 1 cut(s) 344
PleI GAGTC 1 cut(s) 502
PpsI GAGTC 1 cut(s) 502
Ppu21I YACGTR 1 cut(s) 1592
PpuMI RGGWCCY 1 cut(s) 306
PscI ACATGT 1 cut(s) 1870
PshBI ATTAAT 1 cut(s) 902
Psp124BI GAGCTC 2 cut(s) 133, 437
Psp1406I AACGTT 1 cut(s) 501
Psp5II RGGWCCY 1 cut(s) 306
Psp6I CCWGG 2 cut(s) 983, 1761
PspGI CCWGG 2 cut(s) 983, 1761
PspN4I GGNNCC 1 cut(s) 308
PspPI GGNCC 2 cut(s) 306, 907
PspPPI RGGWCCY 1 cut(s) 306
PsrI GAACNNNNNNTAC 2 cut(s) 1843, 1875
PstI CTGCAG 1 cut(s) 1277
PstNI CAGNNNCTG 1 cut(s) 1061
PvuI CGATCG 1 cut(s) 344
RsaI GTAC 4 cut(s) 142, 1042, 1054, 1121
RsaNI GTAC 4 cut(s) 141, 1041, 1053, 1120
SacI GAGCTC 2 cut(s) 133, 437
SaqAI TTAA 4 cut(s) 627, 902, 945, 1386
SatI GCNGC 3 cut(s) 207, 1410, 1413
Sau3AI GATC 3 cut(s) 247, 341, 679
Sau96I GGNCC 2 cut(s) 306, 907
ScaI AGTACT 1 cut(s) 1121
SchI GAGTC 1 cut(s) 502
ScrFI CCNGG 2 cut(s) 985, 1763
SduI GDGCHC 4 cut(s) 133, 437, 1035, 1260
SfaNI GCATC 4 cut(s) 310, 651, 658, 1813
SfcI CTRYAG 2 cut(s) 972, 1273
SfuI TTCGAA 1 cut(s) 1755
SinI GGWCC 1 cut(s) 306
SmlI CTYRAG 2 cut(s) 935, 1034
SmoI CTYRAG 2 cut(s) 935, 1034
SphI GCATGC 1 cut(s) 1830
SseBI AGGCCT 1 cut(s) 1370
SsiI CCGC 3 cut(s) 251, 438, 772
SspI AATATT 1 cut(s) 857
SspMI CTAG 3 cut(s) 804, 1044, 1581
SstI GAGCTC 2 cut(s) 133, 437
StuI AGGCCT 1 cut(s) 1370
StyD4I CCNGG 2 cut(s) 983, 1761
StyI CCWWGG 2 cut(s) 219, 1746
TaaI ACNGT 5 cut(s) 869, 1052, 1119, 1249, 1567
TaiI ACGT 4 cut(s) 384, 504, 1490, 1594
TaqI TCGA 4 cut(s) 428, 719, 1755, 1815
TatI WGTACW 2 cut(s) 1052, 1119
Tru1I TTAA 4 cut(s) 627, 902, 945, 1386
Tru9I TTAA 4 cut(s) 627, 902, 945, 1386
TscAI CASTG 5 cut(s) 783, 874, 1187, 1282, 1368
TseFI GTSAC 3 cut(s) 524, 774, 1483
TseI GCWGC 3 cut(s) 206, 1409, 1412
Tsp45I GTSAC 3 cut(s) 524, 774, 1483
TspGWI ACGGA 3 cut(s) 1278, 1802, 1833
TspRI CASTG 5 cut(s) 783, 874, 1187, 1282, 1368
VpaK11BI GGWCC 1 cut(s) 306
VspI ATTAAT 1 cut(s) 902
XapI RAATTY 3 cut(s) 553, 1128, 1320
XbaI TCTAGA 2 cut(s) 803, 1580
XceI RCATGY 2 cut(s) 1830, 1874
XmnI GAANNNNTTC 1 cut(s) 725
XspI CTAG 3 cut(s) 804, 1044, 1581
ZraI GACGTC 1 cut(s) 1488
ZrmI AGTACT 1 cut(s) 1121
Zsp2I ATGCAT 2 cut(s) 325, 1828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.