pycom10g22170

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0028180
Physical Location & Seq
Reverse (-)
24618149 .. 24618565
417 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 417 bp
ATGGCCATTTATACCCTTTCTGTCATCGCCGGCCAATCAGCTGCAATGCTGTTGGCAAGATTATACGGGACAAAGGTAGGCAAAAGCAAATGGCTGGGAGCCCTAGTGCAACTTGCTGCATTCCATATCCTCCTTCCGTCTTATTTCTTCCCAACAAAAAGAAAAAATCCAACCACAAACATTAGTACTAGTACTACTACCGAATCAAAAGAACCCTCGACTTTAATCCTTGCATCAATTTATGTCACTCTTGGCCTTCTAATAGCCTTGGGCTGCTTCTTGTACTCAGTTGGAATATCTTACCTTCCCATCTCTACTTATTCCCTCATCTCTGTGCATCCCGATTGGCATTCAACGGGCTATTCTCCTTCTCCCTCAACTCGTAAAAGTTCACCCCTTACATTGTCAATGCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

139

Amino Acids

14.89

Weight (kDa)

9.72

Isoelectric Point (pI)

46.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 2 - 111 9e-24 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028180)

Species Orthologous Gene IDs
malus_domestica MD10G1265900.v1.1
pyrus_communis pycom10g22170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 2 cut(s) 3, 31
AfaI GTAC 3 cut(s) 187, 193, 284
AgsI TTSAA 1 cut(s) 354
AhlI ACTAGT 1 cut(s) 188
AluBI AGCT 1 cut(s) 41
AluI AGCT 1 cut(s) 41
AoxI GGCC 3 cut(s) 3, 31, 253
ApeKI GCWGC 3 cut(s) 41, 116, 273
AsuHPI GGTGA 1 cut(s) 384
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 103
BbvI GCAGC 3 cut(s) 28, 103, 260
BccI CCATC 1 cut(s) 317
BcuI ACTAGT 1 cut(s) 188
BfaI CTAG 3 cut(s) 104, 189, 415
BisI GCNGC 3 cut(s) 42, 117, 274
BlsI GCNGC 3 cut(s) 43, 118, 275
BmcAI AGTACT 2 cut(s) 187, 193
BmiI GGNNCC 1 cut(s) 100
BmsI GCATC 2 cut(s) 242, 346
BsaJI CCNNGG 1 cut(s) 267
BsaXI ACNNNNNCTCC 2 cut(s) 90, 120
Bse118I RCCGGY 1 cut(s) 29
Bse3DI GCAATG 1 cut(s) 51
BseDI CCNNGG 1 cut(s) 267
BseGI GGATG 1 cut(s) 337
BseMI GCAATG 1 cut(s) 51
BseMII CTCAG 1 cut(s) 300
BseXI GCAGC 3 cut(s) 28, 103, 260
BseYI CCCAGC 1 cut(s) 94
BshFI GGCC 3 cut(s) 5, 33, 255
BsiSI CCGG 1 cut(s) 30
BslFI GGGAC 1 cut(s) 82
BsmFI GGGAC 1 cut(s) 82
BsmI GAATGC 2 cut(s) 119, 349
BsnI GGCC 3 cut(s) 5, 33, 255
Bsp1286I GDGCHC 1 cut(s) 103
BspANI GGCC 3 cut(s) 5, 33, 255
BspCNI CTCAG 1 cut(s) 299
BspLI GGNNCC 1 cut(s) 100
BsrDI GCAATG 1 cut(s) 51
BsrFI RCCGGY 1 cut(s) 29
BssAI RCCGGY 1 cut(s) 29
BssECI CCNNGG 1 cut(s) 267
BssT1I CCWWGG 1 cut(s) 267
BstC8I GCNNGC 1 cut(s) 31
BstDEI CTNAG 1 cut(s) 286
BstF5I GGATG 1 cut(s) 337
BstV1I GCAGC 3 cut(s) 28, 103, 260
BsuRI GGCC 3 cut(s) 5, 33, 255
BtgZI GCGATG 1 cut(s) 10
BtsCI GGATG 1 cut(s) 337
Cac8I GCNNGC 1 cut(s) 31
Cfr10I RCCGGY 1 cut(s) 29
Csp6I GTAC 3 cut(s) 186, 192, 283
CviJI RGCY 9 cut(s) 5, 33, 41, 94, 101, 255, 266, 273, 360
CviKI_1 RGCY 9 cut(s) 5, 33, 41, 94, 101, 255, 266, 273, 360
CviQI GTAC 3 cut(s) 186, 192, 283
DdeI CTNAG 1 cut(s) 286
EaeI YGGCCR 2 cut(s) 3, 31
Eco130I CCWWGG 1 cut(s) 267
Eco24I GRGCYC 1 cut(s) 103
EcoT14I CCWWGG 1 cut(s) 267
EcoT38I GRGCYC 1 cut(s) 103
ErhI CCWWGG 1 cut(s) 267
FaiI YATR 4 cut(s) 12, 64, 126, 243
FaqI GGGAC 1 cut(s) 82
Fnu4HI GCNGC 3 cut(s) 42, 117, 274
FokI GGATG 1 cut(s) 324
FriOI GRGCYC 1 cut(s) 103
Fsp4HI GCNGC 3 cut(s) 42, 117, 274
FspBI CTAG 3 cut(s) 104, 189, 415
GluI GCNGC 3 cut(s) 42, 117, 274
GsaI CCCAGC 1 cut(s) 98
HaeIII GGCC 3 cut(s) 5, 33, 255
HapII CCGG 1 cut(s) 30
HinfI GANTC 1 cut(s) 203
HpaII CCGG 1 cut(s) 30
HphI GGTGA 1 cut(s) 384
Hpy166II GTNNAC 1 cut(s) 392
Hpy188III TCNNGA 1 cut(s) 341
Hpy8I GTNNAC 1 cut(s) 392
HpyAV CCTTC 4 cut(s) 143, 266, 314, 378
HpyCH4V TGCA 5 cut(s) 44, 109, 119, 233, 337
HpyF3I CTNAG 1 cut(s) 286
KroI GCCGGC 1 cut(s) 29
KroNI GCCGGC 1 cut(s) 31
LmnI GCTCC 1 cut(s) 98
LpnPI CCDG 2 cut(s) 43, 80
Lsp1109I GCAGC 3 cut(s) 28, 103, 260
LweI GCATC 2 cut(s) 242, 346
MaeI CTAG 3 cut(s) 104, 189, 415
MaeIII GTNAC 1 cut(s) 244
MboII GAAGA 1 cut(s) 139
MhlI GDGCHC 1 cut(s) 103
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 237
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 2 cut(s) 194, 271
MnlI CCTC 4 cut(s) 140, 226, 335, 385
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 29
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 224
MslI CAYNNNNRTG 1 cut(s) 332
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 41
MspI CCGG 1 cut(s) 30
Mva1269I GAATGC 2 cut(s) 119, 349
NaeI GCCGGC 1 cut(s) 31
NgoMIV GCCGGC 1 cut(s) 29
NlaIV GGNNCC 1 cut(s) 100
NmuCI GTSAC 1 cut(s) 244
PctI GAATGC 2 cut(s) 119, 349
PdiI GCCGGC 1 cut(s) 31
PfeI GAWTC 1 cut(s) 203
PkrI GCNGC 3 cut(s) 43, 118, 275
PspFI CCCAGC 1 cut(s) 94
PspN4I GGNNCC 1 cut(s) 100
PvuII CAGCTG 1 cut(s) 41
RsaI GTAC 3 cut(s) 187, 193, 284
RsaNI GTAC 3 cut(s) 186, 192, 283
RseI CAYNNNNRTG 1 cut(s) 332
SaqAI TTAA 1 cut(s) 224
SatI GCNGC 3 cut(s) 42, 117, 274
ScaI AGTACT 2 cut(s) 187, 193
SduI GDGCHC 1 cut(s) 103
SetI ASST 3 cut(s) 43, 78, 306
SfaNI GCATC 2 cut(s) 242, 346
SmiMI CAYNNNNRTG 1 cut(s) 332
SpeI ACTAGT 1 cut(s) 188
Sse9I AATT 1 cut(s) 237
SspMI CTAG 3 cut(s) 104, 189, 415
StyI CCWWGG 1 cut(s) 267
TaqI TCGA 1 cut(s) 218
TasI AATT 1 cut(s) 237
TatI WGTACW 3 cut(s) 185, 191, 282
TfiI GAWTC 1 cut(s) 203
Tru1I TTAA 1 cut(s) 224
Tru9I TTAA 1 cut(s) 224
TseFI GTSAC 1 cut(s) 244
TseI GCWGC 3 cut(s) 41, 116, 273
Tsp45I GTSAC 1 cut(s) 244
TspGWI ACGGA 1 cut(s) 126
XspI CTAG 3 cut(s) 104, 189, 415
ZrmI AGTACT 2 cut(s) 187, 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.