pycom10g24120

guanylate-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0029286
Physical Location & Seq
Forward (+)
26011575 .. 26012926
1352 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 528 bp
ATGATGAAATTCTTCAGAGGCAAAGACGATTCTTCACTGCCGTCGCCGTCGCAGTCGTTCTCTCCATCGCCTTCGCGGTCGGTGACCGGTCCCCCGAGGCCGATACGGCTTGTGTACTGCGACGAGAATGGGAAGTTCCGGATGGATCAGGAGGCTGTCTCCATGCTGCAGCTCGTAAAGGAGCCCATTGGTATCGTCGCGGTCTGCGGTCGTGCCCGCCAGGGCAAGAGCTTTATTCTGAACCAGCTCCTCGGAAGGAGTGGCGGATTTCAAGTAGCATCTACACATCGCCCATGTACAAAAGGGCTATGGTTGTGGAGTGCACCCTTTAAGAGAACTGCACTGGATGGGACTGAATACAGTCTATTACTATTGGATACTGAAGGAATAGATGCTTTTGATCAGACGGAAACGTACAGCACACAGATATTTTCCTTAGCGGTTCTCCTATCTAGCATGTTTATTTACAACCAGATGGGTGGTATAGATGAAGGTTCCCTTGATCGATCTCTCTTGTCACTCAAATGA

Protein Analysis

176

Amino Acids

19.34

Weight (kDa)

8.47

Isoelectric Point (pI)

51.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GBP PF02263 44 - 169 9.5e-33 Guanylate-binding protein, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0029286)

Species Orthologous Gene IDs
pyrus_communis pycom05g28590 pycom10g24120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 76, 200
AccIII TCCGGA 1 cut(s) 138
AciI CCGC 6 cut(s) 76, 200, 207, 217, 264, 440
AclWI GGATC 1 cut(s) 153
AcsI RAATTY 1 cut(s) 8
AcuI CTGAAG 1 cut(s) 402
AfaI GTAC 3 cut(s) 116, 298, 416
AgeI ACCGGT 1 cut(s) 86
AgsI TTSAA 1 cut(s) 272
AjnI CCWGG 1 cut(s) 219
AluBI AGCT 3 cut(s) 172, 231, 247
AluI AGCT 3 cut(s) 172, 231, 247
Alw21I GWGCWC 1 cut(s) 325
Alw26I GTCTC 1 cut(s) 163
Alw44I GTGCAC 1 cut(s) 321
AlwI GGATC 1 cut(s) 153
Ama87I CYCGRG 1 cut(s) 94
Aor13HI TCCGGA 1 cut(s) 138
AoxI GGCC 1 cut(s) 98
ApaLI GTGCAC 1 cut(s) 321
ApeKI GCWGC 2 cut(s) 166, 169
ApoI RAATTY 1 cut(s) 8
AsiGI ACCGGT 1 cut(s) 86
Asp700I GAANNNNTTC 1 cut(s) 11
AspS9I GGNCC 1 cut(s) 89
AsuHPI GGTGA 1 cut(s) 94
AvaI CYCGRG 1 cut(s) 94
AvaII GGWCC 1 cut(s) 89
BaeGI GKGCMC 2 cut(s) 217, 325
BanII GRGCYC 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 325
BbvI GCAGC 2 cut(s) 153, 181
BccI CCATC 4 cut(s) 73, 136, 341, 469
BceAI ACGGC 3 cut(s) 25, 31, 122
BciT130I CCWGG 1 cut(s) 221
BciVI GTATCC 1 cut(s) 370
BclI TGATCA 1 cut(s) 400
BcoDI GTCTC 1 cut(s) 163
BfaI CTAG 1 cut(s) 453
BfmI CTRYAG 1 cut(s) 167
BfuI GTATCC 1 cut(s) 370
BglI GCCNNNNNGGC 1 cut(s) 106
BisI GCNGC 2 cut(s) 167, 170
BlsI GCNGC 2 cut(s) 168, 171
Bme1390I CCNGG 1 cut(s) 221
Bme18I GGWCC 1 cut(s) 89
BmeT110I CYCGRG 1 cut(s) 94
BmgT120I GGNCC 1 cut(s) 89
BmiI GGNNCC 3 cut(s) 91, 183, 496
BmrFI CCNGG 1 cut(s) 221
BmsI GCATC 2 cut(s) 287, 382
BplI GAGNNNNNCTC 2 cut(s) 143, 175
Bpu10I CCTNAGC 1 cut(s) 436
Bsa29I ATCGAT 1 cut(s) 505
BsaJI CCNNGG 3 cut(s) 95, 220, 250
BsaWI WCCGGW 2 cut(s) 86, 138
Bse118I RCCGGY 1 cut(s) 86
Bse1I ACTGG 1 cut(s) 348
BseAI TCCGGA 1 cut(s) 138
BseBI CCWGG 1 cut(s) 221
BseCI ATCGAT 1 cut(s) 505
BseDI CCNNGG 3 cut(s) 95, 220, 250
BseGI GGATG 2 cut(s) 147, 352
BseNI ACTGG 1 cut(s) 348
BseRI GAGGAG 1 cut(s) 239
BseSI GKGCMC 2 cut(s) 217, 325
BseXI GCAGC 2 cut(s) 153, 181
BsgI GTGCAG 1 cut(s) 324
Bsh1236I CGCG 2 cut(s) 76, 200
Bsh1285I CGRYCG 2 cut(s) 80, 211
BshFI GGCC 1 cut(s) 100
BshTI ACCGGT 1 cut(s) 86
BshVI ATCGAT 1 cut(s) 505
BsiEI CGRYCG 2 cut(s) 80, 211
BsiHKAI GWGCWC 1 cut(s) 325
BsiHKCI CYCGRG 1 cut(s) 94
BsiSI CCGG 2 cut(s) 87, 139
BslFI GGGAC 2 cut(s) 75, 364
BsmAI GTCTC 1 cut(s) 163
BsmFI GGGAC 2 cut(s) 75, 364
BsnI GGCC 1 cut(s) 100
BsoBI CYCGRG 1 cut(s) 94
Bsp1286I GDGCHC 3 cut(s) 186, 217, 325
Bsp13I TCCGGA 1 cut(s) 138
Bsp1407I TGTACA 1 cut(s) 296
Bsp143I GATC 4 cut(s) 145, 400, 502, 506
BspACI CCGC 6 cut(s) 76, 200, 207, 217, 264, 440
BspANI GGCC 1 cut(s) 100
BspDI ATCGAT 1 cut(s) 505
BspEI TCCGGA 1 cut(s) 138
BspFNI CGCG 2 cut(s) 76, 200
BspLI GGNNCC 3 cut(s) 91, 183, 496
BspMAI CTGCAG 1 cut(s) 171
BspPI GGATC 1 cut(s) 153
BsrFI RCCGGY 1 cut(s) 86
BsrGI TGTACA 1 cut(s) 296
BsrI ACTGG 1 cut(s) 348
BssAI RCCGGY 1 cut(s) 86
BssECI CCNNGG 3 cut(s) 95, 220, 250
BssMI GATC 4 cut(s) 145, 400, 502, 506
Bst2UI CCWGG 1 cut(s) 221
Bst4CI ACNGT 1 cut(s) 362
BstAUI TGTACA 1 cut(s) 296
BstC8I GCNNGC 1 cut(s) 217
BstDEI CTNAG 1 cut(s) 436
BstEII GGTNACC 1 cut(s) 82
BstF5I GGATG 2 cut(s) 147, 352
BstFNI CGCG 2 cut(s) 76, 200
BstKTI GATC 4 cut(s) 148, 403, 505, 509
BstMAI GTCTC 1 cut(s) 163
BstMBI GATC 4 cut(s) 145, 400, 502, 506
BstMCI CGRYCG 2 cut(s) 80, 211
BstMWI GCNNNNNNNGC 1 cut(s) 106
BstNI CCWGG 1 cut(s) 221
BstNSI RCATGY 1 cut(s) 460
BstPI GGTNACC 1 cut(s) 82
BstSCI CCNGG 1 cut(s) 219
BstSFI CTRYAG 1 cut(s) 167
BstSLI GKGCMC 2 cut(s) 217, 325
BstUI CGCG 2 cut(s) 76, 200
BstV1I GCAGC 2 cut(s) 153, 181
BstXI CCANNNNNNTGG 1 cut(s) 479
Bsu15I ATCGAT 1 cut(s) 505
BsuI GTATCC 1 cut(s) 370
BsuRI GGCC 1 cut(s) 100
BsuTUI ATCGAT 1 cut(s) 505
BtgZI GCGATG 2 cut(s) 51, 272
BtsCI GGATG 2 cut(s) 147, 352
BtsI GCAGTG 1 cut(s) 35
BtsIMutI CAGTG 2 cut(s) 35, 341
Cac8I GCNNGC 1 cut(s) 217
Cfr10I RCCGGY 1 cut(s) 86
Cfr13I GGNCC 1 cut(s) 89
ClaI ATCGAT 1 cut(s) 505
Csp6I GTAC 3 cut(s) 115, 297, 415
CspAI ACCGGT 1 cut(s) 86
CviAII CATG 3 cut(s) 163, 294, 457
CviJI RGCY 8 cut(s) 100, 109, 155, 172, 184, 231, 247, 307
CviKI_1 RGCY 8 cut(s) 100, 109, 155, 172, 184, 231, 247, 307
CviQI GTAC 3 cut(s) 115, 297, 415
DdeI CTNAG 1 cut(s) 436
DpnI GATC 4 cut(s) 147, 402, 504, 508
DpnII GATC 4 cut(s) 145, 400, 502, 506
EciI GGCGGA 1 cut(s) 279
Eco24I GRGCYC 1 cut(s) 186
Eco47I GGWCC 1 cut(s) 89
Eco57I CTGAAG 1 cut(s) 402
Eco88I CYCGRG 1 cut(s) 94
Eco91I GGTNACC 1 cut(s) 82
EcoO65I GGTNACC 1 cut(s) 82
EcoRII CCWGG 1 cut(s) 219
EcoT38I GRGCYC 1 cut(s) 186
FaeI CATG 3 cut(s) 166, 297, 460
FaiI YATR 5 cut(s) 164, 295, 310, 458, 485
FalI AAGNNNNNCTT 2 cut(s) 483, 515
FaqI GGGAC 2 cut(s) 75, 364
FatI CATG 3 cut(s) 162, 293, 456
FauI CCCGC 1 cut(s) 224
FbaI TGATCA 1 cut(s) 400
Fnu4HI GCNGC 2 cut(s) 167, 170
FokI GGATG 2 cut(s) 154, 359
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 2 cut(s) 167, 170
FspBI CTAG 1 cut(s) 453
GluI GCNGC 2 cut(s) 167, 170
HaeIII GGCC 1 cut(s) 100
HapII CCGG 2 cut(s) 87, 139
Hin1II CATG 3 cut(s) 166, 297, 460
HinfI GANTC 1 cut(s) 29
HpaII CCGG 2 cut(s) 87, 139
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 2 cut(s) 115, 323
Hpy188I TCNGA 4 cut(s) 17, 240, 254, 405
Hpy188III TCNNGA 2 cut(s) 139, 149
Hpy8I GTNNAC 2 cut(s) 115, 323
Hpy99I CGWCG 4 cut(s) 46, 52, 125, 200
HpyAV CCTTC 4 cut(s) 81, 249, 377, 485
HpyCH4III ACNGT 1 cut(s) 362
HpyCH4IV ACGT 1 cut(s) 413
HpyCH4V TGCA 3 cut(s) 169, 323, 341
HpyF10VI GCNNNNNNNGC 1 cut(s) 106
HpyF3I CTNAG 1 cut(s) 436
HpySE526I ACGT 1 cut(s) 413
Hsp92II CATG 3 cut(s) 166, 297, 460
Kpn2I TCCGGA 1 cut(s) 138
Ksp22I TGATCA 1 cut(s) 400
Kzo9I GATC 4 cut(s) 145, 400, 502, 506
LmnI GCTCC 2 cut(s) 181, 252
LpnPI CCDG 8 cut(s) 100, 134, 152, 206, 233, 257, 329, 485
Lsp1109I GCAGC 2 cut(s) 153, 181
LweI GCATC 2 cut(s) 287, 382
MaeI CTAG 1 cut(s) 453
MaeII ACGT 1 cut(s) 413
MaeIII GTNAC 2 cut(s) 82, 516
MalI GATC 4 cut(s) 147, 402, 504, 508
MboI GATC 4 cut(s) 145, 400, 502, 506
MboII GAAGA 2 cut(s) 4, 24
MhlI GDGCHC 3 cut(s) 186, 217, 325
MluCI AATT 1 cut(s) 8
MnlI CCTC 4 cut(s) 11, 90, 145, 260
MroI TCCGGA 1 cut(s) 138
MroXI GAANNNNTTC 1 cut(s) 11
MseI TTAA 1 cut(s) 330
MslI CAYNNNNRTG 1 cut(s) 523
MspI CCGG 2 cut(s) 87, 139
MspR9I CCNGG 1 cut(s) 221
MvaI CCWGG 1 cut(s) 221
MvnI CGCG 2 cut(s) 76, 200
MwoI GCNNNNNNNGC 1 cut(s) 106
NdeII GATC 4 cut(s) 145, 400, 502, 506
NlaIII CATG 3 cut(s) 166, 297, 460
NlaIV GGNNCC 3 cut(s) 91, 183, 496
NmuCI GTSAC 2 cut(s) 82, 516
NspI RCATGY 1 cut(s) 460
PdmI GAANNNNTTC 1 cut(s) 11
PfeI GAWTC 1 cut(s) 29
PinAI ACCGGT 1 cut(s) 86
PkrI GCNGC 2 cut(s) 168, 171
Psp6I CCWGG 1 cut(s) 219
PspEI GGTNACC 1 cut(s) 82
PspGI CCWGG 1 cut(s) 219
PspN4I GGNNCC 3 cut(s) 91, 183, 496
PspPI GGNCC 1 cut(s) 89
PstI CTGCAG 1 cut(s) 171
RsaI GTAC 3 cut(s) 116, 298, 416
RsaNI GTAC 3 cut(s) 115, 297, 415
RseI CAYNNNNRTG 1 cut(s) 523
SaqAI TTAA 1 cut(s) 330
SatI GCNGC 2 cut(s) 167, 170
Sau3AI GATC 4 cut(s) 145, 400, 502, 506
Sau96I GGNCC 1 cut(s) 89
ScrFI CCNGG 1 cut(s) 221
SduI GDGCHC 3 cut(s) 186, 217, 325
SetI ASST 5 cut(s) 174, 233, 249, 416, 496
SfaNI GCATC 2 cut(s) 287, 382
SfcI CTRYAG 1 cut(s) 167
SinI GGWCC 1 cut(s) 89
SmiMI CAYNNNNRTG 1 cut(s) 523
Sse9I AATT 1 cut(s) 8
SsiI CCGC 6 cut(s) 76, 200, 207, 217, 264, 440
SspMI CTAG 1 cut(s) 453
StyD4I CCNGG 1 cut(s) 219
TaaI ACNGT 1 cut(s) 362
TaiI ACGT 1 cut(s) 416
TaqI TCGA 1 cut(s) 505
TasI AATT 1 cut(s) 8
TatI WGTACW 2 cut(s) 114, 296
TfiI GAWTC 1 cut(s) 29
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TscAI CASTG 2 cut(s) 42, 348
TseFI GTSAC 2 cut(s) 82, 516
TseI GCWGC 2 cut(s) 166, 169
Tsp45I GTSAC 2 cut(s) 82, 516
TspDTI ATGAA 2 cut(s) 20, 504
TspGWI ACGGA 1 cut(s) 422
TspRI CASTG 2 cut(s) 42, 348
VneI GTGCAC 1 cut(s) 321
VpaK11BI GGWCC 1 cut(s) 89
XapI RAATTY 1 cut(s) 8
XceI RCATGY 1 cut(s) 460
XmnI GAANNNNTTC 1 cut(s) 11
XspI CTAG 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.