pycom10g26920

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0029288
Physical Location & Seq
Forward (+)
28082070 .. 28083232
1163 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 633 bp
ATGTGTGCCAATGAGGATGGGGATCTGTTAATAGAGTCTGCAGTGAAAAACATTCCCAAAGTTTTTGTTGTATCAACTTTGGTGAGTGTGATGCCAGCTCAGCCTTTCCTATTCCGAAATTATCAGTATCCTGCGGGAACACTAGAGGTGCCTCCTGCAATTTCAGAGAGTTCAGGAATAACTGGACCACCTCCTGGATGTGCTGAAGGCTATAAGCGCAGTGCCTTTATAGGAAGCTGTAAGCATCAAGTATGGCAAGCTATAAGAGCATCATCTGCTGCTCCGTATTATCTTGATGATTTCTCAGATGATGTGAATCGTTGGCAAGATGGTGCAATAGTGGCAAACAATCCAACGATCTTTTCCATTAGAGAAGCACAACTTTTATGGCCAGACACAAAAATTGACTGCTTAGTTTCCATTGGCTGTGGTTCTGTTCCAACGAAGGTGCGAAAAGGTGGTTGGCGTTATCTGGATACTGGTCAGGTATTGATAGAAAGTGCATGCTCTGTGGAACGGGTTGAGGAAGCTTTGAGCACACTGCTACCCATGCTCCCTGGAATACAATATTTTCGGTTTAATCCAGTTGATGAACGCTGTGATATGGAACTGGATGAGACTGATCGATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001516 GO:0001676 GO:0003674 GO:0003824 GO:0004620 GO:0004623 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006636 GO:0006644 GO:0006650 GO:0006690 GO:0006692 GO:0006693 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008219 GO:0008610 GO:0009056 GO:0009058 GO:0009395 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009620 GO:0009694 GO:0009695 GO:0009987 GO:0010817 GO:0010876 GO:0015711 GO:0015718 GO:0015849 GO:0015908 GO:0015909 GO:0016020 GO:0016042 GO:0016053 GO:0016298 GO:0016787 GO:0016788 GO:0019369 GO:0019637 GO:0019752 GO:0031090 GO:0031903 GO:0032309 GO:0032787 GO:0033036 GO:0033559 GO:0034638 GO:0042445 GO:0042446 GO:0042579 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043651 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0046337 GO:0046338 GO:0046394 GO:0046434 GO:0046456 GO:0046457 GO:0046470 GO:0046475 GO:0046486 GO:0046503 GO:0046717 GO:0046903 GO:0046942 GO:0047372 GO:0047499 GO:0050482 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0052689 GO:0065007 GO:0065008 GO:0071702 GO:0071704 GO:0071715 GO:0072330 GO:0097164 GO:0098542 GO:0098588 GO:0098805 GO:1901564 GO:1901565 GO:1901568 GO:1901570 GO:1901571 GO:1901575 GO:1901576 GO:1903963
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm LRR_8 Patatin

Protein Analysis

211

Amino Acids

23.13

Weight (kDa)

4.64

Isoelectric Point (pI)

47.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 82 - 119 3.4e-06 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029288)

Species Orthologous Gene IDs
pyrus_communis pycom05g31220 pycom10g26920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 148
AccB7I CCANNNNNTGG 1 cut(s) 194
AciI CCGC 1 cut(s) 134
AclWI GGATC 1 cut(s) 30
AcoI YGGCCR 1 cut(s) 389
AcuI CTGAAG 1 cut(s) 225
AfiI CCNNNNNNNGG 1 cut(s) 194
AjnI CCWGG 2 cut(s) 193, 556
AjuI GAANNNNNNNTTGG 4 cut(s) 346, 378, 445, 477
AluBI AGCT 4 cut(s) 98, 237, 260, 530
AluI AGCT 4 cut(s) 98, 237, 260, 530
Alw21I GWGCWC 1 cut(s) 539
Alw26I GTCTC 1 cut(s) 611
AlwI GGATC 1 cut(s) 30
AoxI GGCC 1 cut(s) 389
ApeKI GCWGC 1 cut(s) 278
AspLEI GCGC 1 cut(s) 219
AspS9I GGNCC 1 cut(s) 185
AsuHPI GGTGA 1 cut(s) 94
AvaII GGWCC 1 cut(s) 185
BalI TGGCCA 1 cut(s) 391
BanI GGYRCC 1 cut(s) 148
Bbv12I GWGCWC 1 cut(s) 539
BbvI GCAGC 1 cut(s) 265
BccI CCATC 2 cut(s) 11, 323
BciT130I CCWGG 2 cut(s) 195, 558
BciVI GTATCC 2 cut(s) 138, 469
BcoDI GTCTC 1 cut(s) 611
BfaI CTAG 1 cut(s) 143
BfmI CTRYAG 1 cut(s) 39
BfuI GTATCC 2 cut(s) 138, 469
BisI GCNGC 1 cut(s) 279
BlpI GCTNAGC 1 cut(s) 99
BlsI GCNGC 1 cut(s) 280
Bme1390I CCNGG 2 cut(s) 195, 558
Bme18I GGWCC 1 cut(s) 185
BmgT120I GGNCC 1 cut(s) 185
BmiI GGNNCC 1 cut(s) 150
BmrFI CCNGG 2 cut(s) 195, 558
BmsI GCATC 3 cut(s) 81, 253, 278
Bpu1102I GCTNAGC 1 cut(s) 99
Bsa29I ATCGAT 1 cut(s) 625
BsaBI GATNNNNATC 2 cut(s) 21, 315
BsaJI CCNNGG 1 cut(s) 556
BsaXI ACNNNNNCTCC 2 cut(s) 537, 567
Bsc4I CCNNNNNNNGG 1 cut(s) 194
Bse1I ACTGG 4 cut(s) 187, 484, 584, 615
Bse8I GATNNNNATC 2 cut(s) 21, 315
BseBI CCWGG 2 cut(s) 195, 558
BseCI ATCGAT 1 cut(s) 625
BseDI CCNNGG 1 cut(s) 556
BseGI GGATG 3 cut(s) 22, 203, 619
BseJI GATNNNNATC 2 cut(s) 21, 315
BseLI CCNNNNNNNGG 1 cut(s) 194
BseMII CTCAG 2 cut(s) 113, 318
BseNI ACTGG 4 cut(s) 187, 484, 584, 615
BseXI GCAGC 1 cut(s) 265
BshFI GGCC 1 cut(s) 391
BshNI GGYRCC 1 cut(s) 148
BshVI ATCGAT 1 cut(s) 625
BsiHKAI GWGCWC 1 cut(s) 539
BslI CCNNNNNNNGG 1 cut(s) 194
BsmAI GTCTC 1 cut(s) 611
BsnI GGCC 1 cut(s) 391
Bsp1286I GDGCHC 1 cut(s) 539
Bsp143I GATC 4 cut(s) 22, 357, 622, 626
Bsp1720I GCTNAGC 1 cut(s) 99
BspACI CCGC 1 cut(s) 134
BspANI GGCC 1 cut(s) 391
BspCNI CTCAG 2 cut(s) 112, 317
BspDI ATCGAT 1 cut(s) 625
BspLI GGNNCC 1 cut(s) 150
BspMAI CTGCAG 1 cut(s) 43
BspPI GGATC 1 cut(s) 30
BspT107I GGYRCC 1 cut(s) 148
BsrI ACTGG 4 cut(s) 187, 484, 584, 615
BssECI CCNNGG 1 cut(s) 556
BssMI GATC 4 cut(s) 22, 357, 622, 626
Bst2UI CCWGG 2 cut(s) 195, 558
BstAPI GCANNNNNTGC 1 cut(s) 275
BstC8I GCNNGC 3 cut(s) 96, 258, 505
BstDEI CTNAG 3 cut(s) 99, 304, 412
BstF5I GGATG 3 cut(s) 22, 203, 619
BstHHI GCGC 1 cut(s) 219
BstKTI GATC 4 cut(s) 25, 360, 625, 629
BstMAI GTCTC 1 cut(s) 611
BstMBI GATC 4 cut(s) 22, 357, 622, 626
BstMWI GCNNNNNNNGC 6 cut(s) 100, 216, 266, 275, 341, 550
BstNI CCWGG 2 cut(s) 195, 558
BstNSI RCATGY 1 cut(s) 507
BstSCI CCNGG 2 cut(s) 193, 556
BstSFI CTRYAG 1 cut(s) 39
BstV1I GCAGC 1 cut(s) 265
BstX2I RGATCY 1 cut(s) 22
BstYI RGATCY 1 cut(s) 22
Bsu15I ATCGAT 1 cut(s) 625
BsuI GTATCC 2 cut(s) 138, 469
BsuRI GGCC 1 cut(s) 391
BsuTUI ATCGAT 1 cut(s) 625
BtsCI GGATG 3 cut(s) 22, 203, 619
BtsI GCAGTG 3 cut(s) 48, 226, 539
BtsIMutI CAGTG 3 cut(s) 48, 226, 539
Cac8I GCNNGC 3 cut(s) 96, 258, 505
CfoI GCGC 1 cut(s) 219
Cfr13I GGNCC 1 cut(s) 185
ClaI ATCGAT 1 cut(s) 625
CviAII CATG 2 cut(s) 504, 550
CviJI RGCY 8 cut(s) 98, 103, 210, 237, 260, 391, 426, 530
CviKI_1 RGCY 8 cut(s) 98, 103, 210, 237, 260, 391, 426, 530
DdeI CTNAG 3 cut(s) 99, 304, 412
DpnI GATC 4 cut(s) 24, 359, 624, 628
DpnII GATC 4 cut(s) 22, 357, 622, 626
EaeI YGGCCR 1 cut(s) 389
Eco47I GGWCC 1 cut(s) 185
Eco57I CTGAAG 1 cut(s) 225
EcoRII CCWGG 2 cut(s) 193, 556
FaeI CATG 2 cut(s) 507, 553
FaiI YATR 8 cut(s) 213, 230, 253, 263, 388, 505, 551, 605
FalI AAGNNNNNCTT 2 cut(s) 366, 398
FatI CATG 2 cut(s) 503, 549
FauI CCCGC 1 cut(s) 127
Fnu4HI GCNGC 1 cut(s) 279
FokI GGATG 3 cut(s) 29, 210, 626
Fsp4HI GCNGC 1 cut(s) 279
FspBI CTAG 1 cut(s) 143
GlaI GCGC 1 cut(s) 218
GluI GCNGC 1 cut(s) 279
HaeIII GGCC 1 cut(s) 391
HhaI GCGC 1 cut(s) 219
Hin1II CATG 2 cut(s) 507, 553
Hin6I GCGC 1 cut(s) 217
HinP1I GCGC 1 cut(s) 217
HindIII AAGCTT 1 cut(s) 528
HinfI GANTC 2 cut(s) 35, 316
HphI GGTGA 1 cut(s) 94
Hpy188I TCNGA 3 cut(s) 116, 166, 307
Hpy188III TCNNGA 4 cut(s) 174, 293, 473, 630
HpyAV CCTTC 2 cut(s) 200, 439
HpyCH4V TGCA 4 cut(s) 41, 158, 335, 503
HpyF10VI GCNNNNNNNGC 6 cut(s) 100, 216, 266, 275, 341, 550
HpyF3I CTNAG 3 cut(s) 99, 304, 412
Hsp92II CATG 2 cut(s) 507, 553
HspAI GCGC 1 cut(s) 217
Kzo9I GATC 4 cut(s) 22, 357, 622, 626
LmnI GCTCC 2 cut(s) 286, 558
Lsp1109I GCAGC 1 cut(s) 265
LweI GCATC 3 cut(s) 81, 253, 278
MaeI CTAG 1 cut(s) 143
MalI GATC 4 cut(s) 24, 359, 624, 628
MboI GATC 4 cut(s) 22, 357, 622, 626
MflI RGATCY 1 cut(s) 22
MhlI GDGCHC 1 cut(s) 539
MlsI TGGCCA 1 cut(s) 391
MluCI AATT 3 cut(s) 118, 159, 402
MluNI TGGCCA 1 cut(s) 391
MlyI GAGTC 1 cut(s) 44
MmeI TCCRAC 2 cut(s) 377, 464
MnlI CCTC 5 cut(s) 7, 139, 162, 201, 517
Mox20I TGGCCA 1 cut(s) 391
MscI TGGCCA 1 cut(s) 391
MseI TTAA 2 cut(s) 29, 579
Msp20I TGGCCA 1 cut(s) 391
MspR9I CCNGG 2 cut(s) 195, 558
MvaI CCWGG 2 cut(s) 195, 558
MwoI GCNNNNNNNGC 6 cut(s) 100, 216, 266, 275, 341, 550
NdeII GATC 4 cut(s) 22, 357, 622, 626
NlaIII CATG 2 cut(s) 507, 553
NlaIV GGNNCC 1 cut(s) 150
NspI RCATGY 1 cut(s) 507
PaeI GCATGC 1 cut(s) 507
PfeI GAWTC 1 cut(s) 316
PflMI CCANNNNNTGG 1 cut(s) 194
PfoI TCCNGGA 1 cut(s) 193
PkrI GCNGC 1 cut(s) 280
PleI GAGTC 1 cut(s) 43
PpsI GAGTC 1 cut(s) 43
Psp6I CCWGG 2 cut(s) 193, 556
PspGI CCWGG 2 cut(s) 193, 556
PspN4I GGNNCC 1 cut(s) 150
PspPI GGNCC 1 cut(s) 185
PstI CTGCAG 1 cut(s) 43
PsuI RGATCY 1 cut(s) 22
SaqAI TTAA 2 cut(s) 29, 579
SatI GCNGC 1 cut(s) 279
Sau3AI GATC 4 cut(s) 22, 357, 622, 626
Sau96I GGNCC 1 cut(s) 185
SchI GAGTC 1 cut(s) 44
ScrFI CCNGG 2 cut(s) 195, 558
SduI GDGCHC 1 cut(s) 539
SetI ASST 9 cut(s) 100, 150, 193, 239, 262, 450, 460, 489, 532
SfaNI GCATC 3 cut(s) 81, 253, 278
SfcI CTRYAG 1 cut(s) 39
SinI GGWCC 1 cut(s) 185
SphI GCATGC 1 cut(s) 507
Sse9I AATT 3 cut(s) 118, 159, 402
SsiI CCGC 1 cut(s) 134
SspI AATATT 1 cut(s) 569
SspMI CTAG 1 cut(s) 143
StyD4I CCNGG 2 cut(s) 193, 556
TaqI TCGA 1 cut(s) 625
TasI AATT 3 cut(s) 118, 159, 402
TfiI GAWTC 1 cut(s) 316
Tru1I TTAA 2 cut(s) 29, 579
Tru9I TTAA 2 cut(s) 29, 579
TscAI CASTG 3 cut(s) 48, 226, 546
TseI GCWGC 1 cut(s) 278
TspDTI ATGAA 1 cut(s) 606
TspGWI ACGGA 1 cut(s) 273
TspRI CASTG 3 cut(s) 48, 226, 546
Van91I CCANNNNNTGG 1 cut(s) 194
VpaK11BI GGWCC 1 cut(s) 185
XceI RCATGY 1 cut(s) 507
XspI CTAG 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.