pycom111g01850

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0028464
Physical Location & Seq
Forward (+)
1119026 .. 1120087
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 465 bp
ATGTTTGACATAGGGCAAAATGATGTATCTGTTGGTTTTCGGAATATGAACAATAATGAGCAATTTCCAGCTGACATCAAAGACATGATTAACCAGTTGGCTACGGCTGTACGATATCAAAACGACGTTGCTAGACAGTTCAACAAGGTTCTCAACCAGCAAGTGATCCAGCTAAGGACGGAGCTCCCCCTTTCTGCAATAACATATGTCGATGTCTTTTCCGCAAAGTATCAACTCTACGGCAGAGCAAAGGAGCGCGGATTTGGGAATACTGCAAACATCTGCTGTGGATATTTTGAAGATAAGAGCCAAGTGTGGTATGGGCAAAGGGCAAACGTAAATGGGACAGAAGTGTTTGGTGGTTCATGTGAAGATCCTTCCTCGTATGTGAGCTGGGATGGTGTGCACTACACTGATGCTGCAAATCATTTGATTGCTGCTCAAATAATCAACAGTTCATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

155

Amino Acids

17.34

Weight (kDa)

5.03

Isoelectric Point (pI)

27.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 2 - 149 6.6e-11 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0028464)

Species Orthologous Gene IDs
malus_domestica MD09G1022100.v1.1
pyrus_communis pycom111g01850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 258
AciI CCGC 2 cut(s) 222, 258
AclWI GGATC 2 cut(s) 160, 368
AfaI GTAC 1 cut(s) 111
AgsI TTSAA 2 cut(s) 142, 299
AluBI AGCT 4 cut(s) 71, 172, 184, 393
AluI AGCT 4 cut(s) 71, 172, 184, 393
Alw21I GWGCWC 2 cut(s) 186, 408
Alw44I GTGCAC 1 cut(s) 404
AlwI GGATC 2 cut(s) 160, 368
ApaLI GTGCAC 1 cut(s) 404
ApeKI GCWGC 2 cut(s) 419, 437
AspLEI GCGC 1 cut(s) 258
BaeGI GKGCMC 1 cut(s) 408
BanII GRGCYC 1 cut(s) 186
Bbv12I GWGCWC 2 cut(s) 186, 408
BbvI GCAGC 2 cut(s) 406, 424
BccI CCATC 1 cut(s) 392
BceAI ACGGC 2 cut(s) 120, 256
BfaI CTAG 1 cut(s) 132
BisI GCNGC 2 cut(s) 420, 438
BlsI GCNGC 2 cut(s) 421, 439
BmsI GCATC 1 cut(s) 406
Bpu10I CCTNAGC 1 cut(s) 173
Bse1I ACTGG 1 cut(s) 94
BseGI GGATG 1 cut(s) 403
BseNI ACTGG 1 cut(s) 94
BseSI GKGCMC 1 cut(s) 408
BseXI GCAGC 2 cut(s) 406, 424
BseYI CCCAGC 1 cut(s) 393
Bsh1236I CGCG 1 cut(s) 258
BsiHKAI GWGCWC 2 cut(s) 186, 408
BslFI GGGAC 1 cut(s) 358
BsmFI GGGAC 1 cut(s) 358
Bsp1286I GDGCHC 2 cut(s) 186, 408
Bsp143I GATC 2 cut(s) 165, 373
BspACI CCGC 2 cut(s) 222, 258
BspFNI CGCG 1 cut(s) 258
BspPI GGATC 2 cut(s) 160, 368
BsrI ACTGG 1 cut(s) 94
BssMI GATC 2 cut(s) 165, 373
Bst4CI ACNGT 2 cut(s) 138, 455
BstDEI CTNAG 1 cut(s) 173
BstF5I GGATG 1 cut(s) 403
BstFNI CGCG 1 cut(s) 258
BstHHI GCGC 1 cut(s) 258
BstKTI GATC 2 cut(s) 168, 376
BstMBI GATC 2 cut(s) 165, 373
BstSLI GKGCMC 1 cut(s) 408
BstUI CGCG 1 cut(s) 258
BstV1I GCAGC 2 cut(s) 406, 424
BstX2I RGATCY 1 cut(s) 373
BstYI RGATCY 1 cut(s) 373
BtsCI GGATG 1 cut(s) 403
BtsIMutI CAGTG 1 cut(s) 411
CfoI GCGC 1 cut(s) 258
Csp6I GTAC 1 cut(s) 110
CviAII CATG 2 cut(s) 85, 366
CviJI RGCY 7 cut(s) 71, 101, 107, 172, 184, 309, 393
CviKI_1 RGCY 7 cut(s) 71, 101, 107, 172, 184, 309, 393
CviQI GTAC 1 cut(s) 110
DdeI CTNAG 1 cut(s) 173
DpnI GATC 2 cut(s) 167, 375
DpnII GATC 2 cut(s) 165, 373
Ecl136II GAGCTC 1 cut(s) 184
Eco24I GRGCYC 1 cut(s) 186
Eco32I GATATC 1 cut(s) 116
Eco53kI GAGCTC 1 cut(s) 184
EcoICRI GAGCTC 1 cut(s) 184
EcoRV GATATC 1 cut(s) 116
EcoT38I GRGCYC 1 cut(s) 186
FaeI CATG 2 cut(s) 88, 369
FaiI YATR 9 cut(s) 11, 47, 86, 205, 207, 321, 367, 387, 460
FaqI GGGAC 1 cut(s) 358
FatI CATG 2 cut(s) 84, 365
FauNDI CATATG 1 cut(s) 205
Fnu4HI GCNGC 2 cut(s) 420, 438
FokI GGATG 1 cut(s) 410
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 2 cut(s) 420, 438
FspBI CTAG 1 cut(s) 132
GlaI GCGC 1 cut(s) 257
GluI GCNGC 2 cut(s) 420, 438
GsaI CCCAGC 1 cut(s) 397
HhaI GCGC 1 cut(s) 258
Hin1II CATG 2 cut(s) 88, 369
Hin6I GCGC 1 cut(s) 256
HinP1I GCGC 1 cut(s) 256
Hpy166II GTNNAC 1 cut(s) 406
Hpy188I TCNGA 1 cut(s) 42
Hpy8I GTNNAC 1 cut(s) 406
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 1 cut(s) 387
HpyCH4III ACNGT 2 cut(s) 138, 455
HpyCH4IV ACGT 2 cut(s) 126, 336
HpyCH4V TGCA 4 cut(s) 197, 275, 406, 422
HpyF3I CTNAG 1 cut(s) 173
HpySE526I ACGT 2 cut(s) 126, 336
Hsp92II CATG 2 cut(s) 88, 369
HspAI GCGC 1 cut(s) 256
Kzo9I GATC 2 cut(s) 165, 373
LmnI GCTCC 3 cut(s) 181, 189, 253
LpnPI CCDG 5 cut(s) 81, 107, 170, 182, 379
Lsp1109I GCAGC 2 cut(s) 406, 424
LweI GCATC 1 cut(s) 406
MaeI CTAG 1 cut(s) 132
MaeII ACGT 2 cut(s) 126, 336
MalI GATC 2 cut(s) 167, 375
MboI GATC 2 cut(s) 165, 373
MboII GAAGA 2 cut(s) 311, 383
MflI RGATCY 1 cut(s) 373
MhlI GDGCHC 2 cut(s) 186, 408
MluCI AATT 1 cut(s) 62
MnlI CCTC 1 cut(s) 391
MseI TTAA 1 cut(s) 90
MspA1I CMGCKG 1 cut(s) 71
MvnI CGCG 1 cut(s) 258
NdeI CATATG 1 cut(s) 205
NdeII GATC 2 cut(s) 165, 373
NlaIII CATG 2 cut(s) 88, 369
PkrI GCNGC 2 cut(s) 421, 439
Psp124BI GAGCTC 1 cut(s) 186
PspFI CCCAGC 1 cut(s) 393
PsuI RGATCY 1 cut(s) 373
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 1 cut(s) 111
RsaNI GTAC 1 cut(s) 110
SacI GAGCTC 1 cut(s) 186
SaqAI TTAA 1 cut(s) 90
SatI GCNGC 2 cut(s) 420, 438
Sau3AI GATC 2 cut(s) 165, 373
SduI GDGCHC 2 cut(s) 186, 408
SetI ASST 7 cut(s) 73, 129, 150, 174, 186, 339, 395
SfaNI GCATC 1 cut(s) 406
Sse9I AATT 1 cut(s) 62
SsiI CCGC 2 cut(s) 222, 258
SspMI CTAG 1 cut(s) 132
SstI GAGCTC 1 cut(s) 186
TaaI ACNGT 2 cut(s) 138, 455
TaiI ACGT 2 cut(s) 129, 339
TaqI TCGA 1 cut(s) 210
TasI AATT 1 cut(s) 62
Tru1I TTAA 1 cut(s) 90
Tru9I TTAA 1 cut(s) 90
TscAI CASTG 1 cut(s) 418
TseI GCWGC 2 cut(s) 419, 437
TspDTI ATGAA 3 cut(s) 62, 354, 447
TspGWI ACGGA 1 cut(s) 194
TspRI CASTG 1 cut(s) 418
VneI GTGCAC 1 cut(s) 404
XcmI CCANNNNNNNNNTGG 1 cut(s) 317
XspI CTAG 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.