pycom111g03420

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0022356
Physical Location & Seq
Forward (+)
2073764 .. 2075203
1440 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1440 bp
ATGTCTTCGTCTGCGTGGGCTATCTTAAGAGAATTCTGCTTTGCCTACGGTTTTCTAAGACATGTTGATATGCTCTTTCCAGTAGTACTCGGTTCCATTTACTATCCTTACATCCAAATCAAATTCAGTGAATATTCTGGAGAACATAACCTCATGCGCAGTGAAGCCTTCTCCGCTATTGAGAACTACCTGAGGTCCAAAGCTTCCGATGAAGCAAATCGGCTGCGTGGTTGCGTGACAAACAACCAGTCCCTTGTGCTTAGCATGGATGCAAATGAAAAGGTTGCTGACGAGTTTGAAGGAGCCAAAGTATGGTGGGCTTTCGGGAAAACCAATGTTATGCAAAGGAGTTCGTCGCACCATCCAGTTCCTTATGAGAGATGGTACTACAAGCTCACTTTTCATAAGACGCAGAGGGATCTCATTATCGGGCGTTATATCGAACATGTCATGAAAGAGGGCAATGCAATTAAGTTGAGAAACCGGGAAAGGAAGCTTTATACCAACAATGGGGTACGTTGGAGCCATGTTGTGTTTGAGCACCCTGCGACATTTCAGACACTAGCCATGGATCCGGTGAAGAAGCAGAAGATTGTGGACGATTTAATGACATTCAGCAAAGCGGAAGAGTTTTACAAAAGCATTGGGAGGGCATGGAAGCGAGGGTACCTCCTTTATGGCCCCCCGGGAACTGGGAAGTCAACCATGATTGCCGCCATGGCGAATCATTTGGGCTACAATATTTACGATCTTGAGTTGACTTCAGTGAAGAACAACACCGAGCTGAGGAAGCTACTGCTTGAAACATCGAACAAGTCTCTTATTGTGATTGAAGACATTGATTGTTCGCTTGATCTCACTGGGCAAAGGATTAAGAAGAATGATAAAGCGAAAGAGGACAAAGATAAAGGTCATGGAAAGGAAAACGGAGGACAGGAAAAGGAAGCAAATATACTGAGCCAGGTTACTCTTTCAGGGCTTCTGAACTTCATTGACGGGCTCTGGTCAGCCTGTAAAGGAGAGAGGCTGATAGTATTCACCACCAACCATGTGGAGAAACTCGACGCTGCACTCACTCGAAAAGGACGGTTGGACAAGCACATAGAACTATCATACTGCACCTTTGAAGCATTCAAGGTGCTCGCTAGAAACTACCTTAAACTTGAATCCCACCATTTGTTCCCCACGATTTGCGAGTTGCTGGGGAAAACTAATGTGGTTCCAGCTGATGTCGCAGAGCATTTGATGCCCAAGACACTTTCTCAAAGCGCTGACATCTGCCTGCAGAACCTGGTCCAAGCTCTCAAAAAGGAGAAAGAGAACGGAAAGTTAATAGCCGAGGAAGAAAGGAAATACGGTAAATACGGAGCAATCAGCTGGAAGTGCATGACAAGCACACAAGATCATTTGATAGAAGACAAAAAGAGAGCCAAAAATTAG

Protein Analysis

480

Amino Acids

54.9

Weight (kDa)

9.0

Isoelectric Point (pI)

36.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 32 - 108 1.7e-19 Domain associated at C-terminal with AAA
AAA PF00004 223 - 371 2.5e-16 ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540 PF25568 373 - 441 9.2e-29 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022356)

Species Orthologous Gene IDs
malus_domestica MD02G1033500.v1.1 MD09G1044000.v1.1
pyrus_communis pycom02g02750 pycom111g03420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 158
Acc65I GGTACC 1 cut(s) 666
AccB1I GGYRCC 1 cut(s) 666
AccB7I CCANNNNNTGG 1 cut(s) 312
AciI CCGC 3 cut(s) 174, 623, 714
AclWI GGATC 3 cut(s) 426, 566, 579
AcsI RAATTY 2 cut(s) 32, 122
AcuI CTGAAG 1 cut(s) 747
AfaI GTAC 4 cut(s) 87, 386, 516, 668
AfeI AGCGCT 1 cut(s) 1270
AfiI CCNNNNNNNGG 4 cut(s) 312, 510, 692, 786
AflII CTTAAG 1 cut(s) 25
AflIII ACRYGT 2 cut(s) 61, 445
AgsI TTSAA 6 cut(s) 299, 803, 833, 1127, 1135, 1166
AjnI CCWGG 2 cut(s) 960, 1290
AluBI AGCT 8 cut(s) 203, 394, 496, 784, 793, 1226, 1301, 1377
AluI AGCT 8 cut(s) 203, 394, 496, 784, 793, 1226, 1301, 1377
Alw21I GWGCWC 2 cut(s) 543, 1143
Alw26I GTCTC 1 cut(s) 822
AlwI GGATC 3 cut(s) 426, 566, 579
AlwNI CAGNNNCTG 1 cut(s) 1291
Ama87I CYCGRG 1 cut(s) 685
Aor51HI AGCGCT 1 cut(s) 1270
AoxI GGCC 1 cut(s) 679
ApeKI GCWGC 2 cut(s) 223, 1067
ApoI RAATTY 2 cut(s) 32, 122
ArsI GACNNNNNNTTYG 2 cut(s) 233, 265
Asp718I GGTACC 1 cut(s) 666
AspLEI GCGC 2 cut(s) 159, 1271
AspS9I GGNCC 3 cut(s) 195, 680, 1294
AsuC2I CCSGG 3 cut(s) 485, 686, 687
AsuHPI GGTGA 2 cut(s) 589, 1030
AvaI CYCGRG 1 cut(s) 685
AvaII GGWCC 2 cut(s) 195, 1294
AxyI CCTNAGG 1 cut(s) 191
BamHI GGATCC 1 cut(s) 571
BanI GGYRCC 1 cut(s) 666
BanII GRGCYC 1 cut(s) 1002
BarI GAAGNNNNNNTAC 4 cut(s) 650, 682, 936, 968
BbsI GAAGAC 2 cut(s) 840, 1422
Bbv12I GWGCWC 2 cut(s) 543, 1143
BbvCI CCTCAGC 1 cut(s) 785
BbvI GCAGC 2 cut(s) 210, 1054
BccI CCATC 2 cut(s) 369, 375
BciT130I CCWGG 2 cut(s) 962, 1292
BcnI CCSGG 3 cut(s) 485, 686, 687
BcoDI GTCTC 1 cut(s) 822
BfaI CTAG 2 cut(s) 563, 1146
BfmI CTRYAG 1 cut(s) 1283
BfoI RGCGCY 1 cut(s) 1272
BfrI CTTAAG 1 cut(s) 25
BglI GCCNNNNNGGC 1 cut(s) 719
BisI GCNGC 3 cut(s) 224, 714, 1068
BlpI GCTNAGC 1 cut(s) 260
BlsI GCNGC 3 cut(s) 225, 715, 1069
BmcAI AGTACT 1 cut(s) 87
Bme1390I CCNGG 5 cut(s) 485, 686, 687, 962, 1292
Bme18I GGWCC 2 cut(s) 195, 1294
BmeT110I CYCGRG 1 cut(s) 685
BmgT120I GGNCC 3 cut(s) 195, 680, 1294
BmiI GGNNCC 7 cut(s) 94, 304, 524, 573, 668, 682, 1221
BmrFI CCNGG 5 cut(s) 485, 686, 687, 962, 1292
BmrI ACTGGG 2 cut(s) 702, 870
BmsI GCATC 2 cut(s) 259, 1236
BmuI ACTGGG 2 cut(s) 702, 870
BpiI GAAGAC 2 cut(s) 840, 1422
BplI GAGNNNNNCTC 2 cut(s) 654, 686
BpmI CTGGAG 1 cut(s) 159
Bpu10I CCTNAGC 1 cut(s) 785
Bpu1102I GCTNAGC 1 cut(s) 260
BpuEI CTTGAG 1 cut(s) 773
BpuMI CCSGG 3 cut(s) 485, 686, 687
BsaJI CCNNGG 5 cut(s) 567, 684, 685, 717, 1338
BsaWI WCCGGW 1 cut(s) 574
BsaXI ACNNNNNCTCC 2 cut(s) 294, 324
Bsc4I CCNNNNNNNGG 4 cut(s) 312, 510, 692, 786
Bse1I ACTGG 5 cut(s) 80, 247, 365, 697, 865
Bse21I CCTNAGG 1 cut(s) 191
Bse3DI GCAATG 1 cut(s) 469
BseBI CCWGG 2 cut(s) 962, 1292
BseDI CCNNGG 5 cut(s) 567, 684, 685, 717, 1338
BseGI GGATG 3 cut(s) 111, 274, 361
BseLI CCNNNNNNNGG 4 cut(s) 312, 510, 692, 786
BseMI GCAATG 1 cut(s) 469
BseMII CTCAG 3 cut(s) 182, 776, 947
BseNI ACTGG 5 cut(s) 80, 247, 365, 697, 865
BseXI GCAGC 2 cut(s) 210, 1054
BseYI CCCAGC 1 cut(s) 1201
BsgI GTGCAG 2 cut(s) 1053, 1102
BshFI GGCC 1 cut(s) 681
BshNI GGYRCC 1 cut(s) 666
BsiHKAI GWGCWC 2 cut(s) 543, 1143
BsiHKCI CYCGRG 1 cut(s) 685
BsiSI CCGG 3 cut(s) 484, 575, 686
BslFI GGGAC 1 cut(s) 235
BslI CCNNNNNNNGG 4 cut(s) 312, 510, 692, 786
BsmAI GTCTC 1 cut(s) 822
BsmFI GGGAC 1 cut(s) 235
BsmI GAATGC 1 cut(s) 1130
BsnI GGCC 1 cut(s) 681
BsoBI CYCGRG 1 cut(s) 685
Bsp1286I GDGCHC 3 cut(s) 543, 1002, 1143
Bsp143I GATC 5 cut(s) 418, 571, 748, 853, 1402
Bsp1720I GCTNAGC 1 cut(s) 260
Bsp19I CCATGG 2 cut(s) 567, 717
BspACI CCGC 3 cut(s) 174, 623, 714
BspANI GGCC 1 cut(s) 681
BspCNI CTCAG 3 cut(s) 183, 777, 948
BspHI TCATGA 1 cut(s) 450
BspLI GGNNCC 7 cut(s) 94, 304, 524, 573, 668, 682, 1221
BspMAI CTGCAG 1 cut(s) 1287
BspPI GGATC 3 cut(s) 426, 566, 579
BspT107I GGYRCC 1 cut(s) 666
BspTI CTTAAG 1 cut(s) 25
BsrDI GCAATG 1 cut(s) 469
BsrI ACTGG 5 cut(s) 80, 247, 365, 697, 865
BssECI CCNNGG 5 cut(s) 567, 684, 685, 717, 1338
BssMI GATC 5 cut(s) 418, 571, 748, 853, 1402
BssT1I CCWWGG 2 cut(s) 567, 717
Bst2UI CCWGG 2 cut(s) 962, 1292
Bst4CI ACNGT 3 cut(s) 50, 1089, 1358
Bst6I CTCTTC 1 cut(s) 621
BstAFI CTTAAG 1 cut(s) 25
BstAPI GCANNNNNTGC 1 cut(s) 1246
BstC8I GCNNGC 2 cut(s) 1143, 1283
BstDEI CTNAG 5 cut(s) 56, 191, 260, 785, 956
BstDSI CCRYGG 2 cut(s) 567, 717
BstF5I GGATG 3 cut(s) 111, 274, 361
BstH2I RGCGCY 1 cut(s) 1272
BstHHI GCGC 2 cut(s) 159, 1271
BstKTI GATC 5 cut(s) 421, 574, 751, 856, 1405
BstMAI GTCTC 1 cut(s) 822
BstMBI GATC 5 cut(s) 418, 571, 748, 853, 1402
BstMWI GCNNNNNNNGC 7 cut(s) 173, 719, 790, 1232, 1246, 1383, 1392
BstNI CCWGG 2 cut(s) 962, 1292
BstNSI RCATGY 2 cut(s) 65, 449
BstSCI CCNGG 5 cut(s) 483, 684, 685, 960, 1290
BstSFI CTRYAG 1 cut(s) 1283
BstV1I GCAGC 2 cut(s) 210, 1054
BstV2I GAAGAC 2 cut(s) 840, 1422
BstX2I RGATCY 2 cut(s) 418, 571
BstXI CCANNNNNNTGG 1 cut(s) 1051
BstYI RGATCY 2 cut(s) 418, 571
Bsu36I CCTNAGG 1 cut(s) 191
BsuRI GGCC 1 cut(s) 681
BtgI CCRYGG 2 cut(s) 567, 717
BtsCI GGATG 3 cut(s) 111, 274, 361
BtsI GCAGTG 1 cut(s) 166
BtsIMutI CAGTG 4 cut(s) 133, 166, 771, 858
Cac8I GCNNGC 2 cut(s) 1143, 1283
CaiI CAGNNNCTG 1 cut(s) 1291
CciI TCATGA 1 cut(s) 450
CfoI GCGC 2 cut(s) 159, 1271
Cfr13I GGNCC 3 cut(s) 195, 680, 1294
Cfr9I CCCGGG 1 cut(s) 685
CseI GACGC 2 cut(s) 418, 1073
CsiI ACCWGGT 1 cut(s) 1290
Csp6I GTAC 4 cut(s) 86, 385, 515, 667
CviQI GTAC 4 cut(s) 86, 385, 515, 667
DdeI CTNAG 5 cut(s) 56, 191, 260, 785, 956
DpnI GATC 5 cut(s) 420, 573, 750, 855, 1404
DpnII GATC 5 cut(s) 418, 571, 748, 853, 1402
Eam1104I CTCTTC 1 cut(s) 621
EarI CTCTTC 1 cut(s) 621
Eco130I CCWWGG 2 cut(s) 567, 717
Eco24I GRGCYC 1 cut(s) 1002
Eco47I GGWCC 2 cut(s) 195, 1294
Eco47III AGCGCT 1 cut(s) 1270
Eco57I CTGAAG 1 cut(s) 747
Eco81I CCTNAGG 1 cut(s) 191
Eco88I CYCGRG 1 cut(s) 685
EcoRI GAATTC 1 cut(s) 32
EcoRII CCWGG 2 cut(s) 960, 1290
EcoT14I CCWWGG 2 cut(s) 567, 717
EcoT38I GRGCYC 1 cut(s) 1002
ErhI CCWWGG 2 cut(s) 567, 717
FaqI GGGAC 1 cut(s) 235
Fnu4HI GCNGC 3 cut(s) 224, 714, 1068
FokI GGATG 3 cut(s) 98, 281, 348
FriOI GRGCYC 1 cut(s) 1002
Fsp4HI GCNGC 3 cut(s) 224, 714, 1068
FspBI CTAG 2 cut(s) 563, 1146
FspI TGCGCA 1 cut(s) 158
GlaI GCGC 2 cut(s) 158, 1270
GluI GCNGC 3 cut(s) 224, 714, 1068
GsaI CCCAGC 1 cut(s) 1205
GsuI CTGGAG 1 cut(s) 159
HaeII RGCGCY 1 cut(s) 1272
HaeIII GGCC 1 cut(s) 681
HapII CCGG 3 cut(s) 484, 575, 686
HgaI GACGC 2 cut(s) 418, 1073
HhaI GCGC 2 cut(s) 159, 1271
Hin6I GCGC 2 cut(s) 157, 1269
HinP1I GCGC 2 cut(s) 157, 1269
HincII GTYRAC 2 cut(s) 702, 759
HindII GTYRAC 2 cut(s) 702, 759
HindIII AAGCTT 2 cut(s) 201, 494
HinfI GANTC 2 cut(s) 724, 1166
HpaII CCGG 3 cut(s) 484, 575, 686
HphI GGTGA 2 cut(s) 589, 1030
Hpy166II GTNNAC 3 cut(s) 598, 702, 759
Hpy188I TCNGA 3 cut(s) 208, 558, 984
Hpy188III TCNNGA 4 cut(s) 138, 325, 451, 752
Hpy8I GTNNAC 3 cut(s) 598, 702, 759
Hpy99I CGWCG 2 cut(s) 358, 1067
HpyAV CCTTC 2 cut(s) 178, 293
HpyCH4III ACNGT 3 cut(s) 50, 1089, 1358
HpyCH4IV ACGT 1 cut(s) 517
HpyCH4V TGCA 7 cut(s) 272, 343, 467, 1070, 1119, 1285, 1386
HpyF10VI GCNNNNNNNGC 7 cut(s) 173, 719, 790, 1232, 1246, 1383, 1392
HpyF3I CTNAG 5 cut(s) 56, 191, 260, 785, 956
HpySE526I ACGT 1 cut(s) 517
HspAI GCGC 2 cut(s) 157, 1269
KpnI GGTACC 1 cut(s) 670
Kzo9I GATC 5 cut(s) 418, 571, 748, 853, 1402
LmnI GCTCC 3 cut(s) 302, 522, 1367
Lsp1109I GCAGC 2 cut(s) 210, 1054
LweI GCATC 2 cut(s) 259, 1236
MabI ACCWGGT 1 cut(s) 1290
MaeI CTAG 2 cut(s) 563, 1146
MaeII ACGT 1 cut(s) 517
MaeIII GTNAC 2 cut(s) 235, 964
MalI GATC 5 cut(s) 420, 573, 750, 855, 1404
MboI GATC 5 cut(s) 418, 571, 748, 853, 1402
MboII GAAGA 8 cut(s) 592, 601, 638, 781, 845, 889, 1355, 1427
MflI RGATCY 2 cut(s) 418, 571
MhlI GDGCHC 3 cut(s) 543, 1002, 1143
MluCI AATT 4 cut(s) 32, 122, 468, 1435
MmeI TCCRAC 2 cut(s) 500, 1071
MseI TTAA 6 cut(s) 26, 471, 605, 873, 1158, 1331
MspA1I CMGCKG 2 cut(s) 1226, 1377
MspCI CTTAAG 1 cut(s) 25
MspI CCGG 3 cut(s) 484, 575, 686
MspR9I CCNGG 5 cut(s) 485, 686, 687, 962, 1292
Mva1269I GAATGC 1 cut(s) 1130
MvaI CCWGG 2 cut(s) 962, 1292
MwoI GCNNNNNNNGC 7 cut(s) 173, 719, 790, 1232, 1246, 1383, 1392
NciI CCSGG 3 cut(s) 485, 686, 687
NcoI CCATGG 2 cut(s) 567, 717
NdeII GATC 5 cut(s) 418, 571, 748, 853, 1402
NlaIV GGNNCC 7 cut(s) 94, 304, 524, 573, 668, 682, 1221
NmeAIII GCCGAG 1 cut(s) 1363
NmuCI GTSAC 1 cut(s) 235
NsbI TGCGCA 1 cut(s) 158
NspI RCATGY 2 cut(s) 65, 449
PagI TCATGA 1 cut(s) 450
PciI ACATGT 2 cut(s) 61, 445
PctI GAATGC 1 cut(s) 1130
PfeI GAWTC 2 cut(s) 724, 1166
PflMI CCANNNNNTGG 1 cut(s) 312
PkrI GCNGC 3 cut(s) 225, 715, 1069
PscI ACATGT 2 cut(s) 61, 445
Psp6I CCWGG 2 cut(s) 960, 1290
PspFI CCCAGC 1 cut(s) 1201
PspGI CCWGG 2 cut(s) 960, 1290
PspN4I GGNNCC 7 cut(s) 94, 304, 524, 573, 668, 682, 1221
PspPI GGNCC 3 cut(s) 195, 680, 1294
PstI CTGCAG 1 cut(s) 1287
PstNI CAGNNNCTG 1 cut(s) 1291
PsuI RGATCY 2 cut(s) 418, 571
PvuII CAGCTG 2 cut(s) 1226, 1377
RsaI GTAC 4 cut(s) 87, 386, 516, 668
RsaNI GTAC 4 cut(s) 86, 385, 515, 667
SaqAI TTAA 6 cut(s) 26, 471, 605, 873, 1158, 1331
SatI GCNGC 3 cut(s) 224, 714, 1068
Sau3AI GATC 5 cut(s) 418, 571, 748, 853, 1402
Sau96I GGNCC 3 cut(s) 195, 680, 1294
ScaI AGTACT 1 cut(s) 87
ScrFI CCNGG 5 cut(s) 485, 686, 687, 962, 1292
SduI GDGCHC 3 cut(s) 543, 1002, 1143
SexAI ACCWGGT 1 cut(s) 1290
SfaNI GCATC 2 cut(s) 259, 1236
SfcI CTRYAG 1 cut(s) 1283
SinI GGWCC 2 cut(s) 195, 1294
SmaI CCCGGG 1 cut(s) 687
SmlI CTYRAG 2 cut(s) 25, 752
SmoI CTYRAG 2 cut(s) 25, 752
Sse9I AATT 4 cut(s) 32, 122, 468, 1435
SsiI CCGC 3 cut(s) 174, 623, 714
SspI AATATT 2 cut(s) 134, 742
SspMI CTAG 2 cut(s) 563, 1146
StyD4I CCNGG 5 cut(s) 483, 684, 685, 960, 1290
StyI CCWWGG 2 cut(s) 567, 717
TaaI ACNGT 3 cut(s) 50, 1089, 1358
TaiI ACGT 1 cut(s) 520
TaqI TCGA 4 cut(s) 441, 809, 1062, 1078
TasI AATT 4 cut(s) 32, 122, 468, 1435
TatI WGTACW 1 cut(s) 85
TauI GCSGC 1 cut(s) 716
TfiI GAWTC 2 cut(s) 724, 1166
Tru1I TTAA 6 cut(s) 26, 471, 605, 873, 1158, 1331
Tru9I TTAA 6 cut(s) 26, 471, 605, 873, 1158, 1331
TscAI CASTG 4 cut(s) 133, 166, 771, 865
TseFI GTSAC 1 cut(s) 235
TseI GCWGC 2 cut(s) 223, 1067
Tsp45I GTSAC 1 cut(s) 235
TspDTI ATGAA 5 cut(s) 225, 291, 392, 467, 979
TspGWI ACGGA 3 cut(s) 942, 1338, 1380
TspMI CCCGGG 1 cut(s) 685
TspRI CASTG 4 cut(s) 133, 166, 771, 865
Van91I CCANNNNNTGG 1 cut(s) 312
Vha464I CTTAAG 1 cut(s) 25
VpaK11BI GGWCC 2 cut(s) 195, 1294
XapI RAATTY 2 cut(s) 32, 122
XceI RCATGY 2 cut(s) 65, 449
XcmI CCANNNNNNNNNTGG 1 cut(s) 1048
XmaI CCCGGG 1 cut(s) 685
XspI CTAG 2 cut(s) 563, 1146
ZrmI AGTACT 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.