pycom111g04970

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0024661
Physical Location & Seq
Forward (+)
3137514 .. 3138320
807 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 807 bp
ATGAGAGTTGTTGACACATATGATGCCGCGCCATTGTTTGGTTTGAAAAGTTGCTTATCGGGATGCATTTTAGTTGTGGGTTTGTTGATGATGCAGCTAAAGTGTTCGATGAAATTACTGAGAGAGATATTGTTGTGTACACTTCGATTATTACTGGTTATGCTCAAGAGTGGCGACCAACGTGCTTGTGAGGTTTTTGGGTTTGCTCGCCGTATGCAGAGGCAAGGATTGCATCCAAATCGGGTCACTCTTGATAGCTTACTTCAGGCAGCGTCGCAGTTAGAGGCACTGAAAGAAGGACGTTCGGTCCATGGCTATGCTATTAGAAGAGGAATTGGTGGTTCGGATGCAGTGTTCGAAACAAGTCTCGTGGACATGTATAACAAATGTGGATCGATCATCAGTCACCCTAGAATGGCAGCCTGCATTTTTGGCGAAATGGATAGAAAGACTATTGGTTCTTGGAATGTCATGATTGCTGGTTATCTTAAAATGGAGCAACCCTTGGAGGCTTTCCGCCTTTTTTGTAGAGTGATGGGAGAAAATTTTGTGCCGGGTTTGATAACTTTGTCCAATGGGATTCTGTCTTGTGCTTGTTTGAATTATTTGCGTCCTGGAAAGAGTATCCATGGTTACATTATTCGAGATGGTATTCAAATTGATCTATTGGCAGCGACCGCTCTGGTCGATCTCTACTCCAAATCAAACAAGTTGATCCAGTCCAGGGAGCTATTTGATAGAATGGAGAAAAAGGATGCTATATCTTATGATGTGATGATGACGTTCCCCGATAATTATGCTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

269

Amino Acids

29.95

Weight (kDa)

8.99

Isoelectric Point (pI)

38.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024661)

Species Orthologous Gene IDs
pyrus_communis pycom08g18500 pycom08g18510 pycom111g04970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 38
AccBSI CCGCTC 1 cut(s) 680
AccII CGCG 1 cut(s) 29
AciI CCGC 3 cut(s) 27, 517, 678
AclWI GGATC 2 cut(s) 400, 709
AcsI RAATTY 1 cut(s) 544
AcuI CTGAAG 1 cut(s) 248
AfaI GTAC 1 cut(s) 139
AfiI CCNNNNNNNGG 3 cut(s) 38, 415, 724
AflIII ACRYGT 1 cut(s) 375
AgsI TTSAA 3 cut(s) 46, 601, 656
AhdI GACNNNNNGTC 1 cut(s) 305
AjnI CCWGG 2 cut(s) 613, 722
AloI GAACNNNNNNTCC 2 cut(s) 338, 370
AluBI AGCT 3 cut(s) 97, 258, 730
AluI AGCT 3 cut(s) 97, 258, 730
Alw26I GTCTC 1 cut(s) 371
AlwI GGATC 2 cut(s) 400, 709
ApeKI GCWGC 4 cut(s) 94, 269, 419, 671
ApoI RAATTY 1 cut(s) 544
AspLEI GCGC 1 cut(s) 31
AspS9I GGNCC 1 cut(s) 307
AsuC2I CCSGG 1 cut(s) 555
AsuHPI GGTGA 1 cut(s) 398
AsuII TTCGAA 1 cut(s) 357
AvaII GGWCC 1 cut(s) 307
BauI CACGAG 1 cut(s) 368
BbvI GCAGC 4 cut(s) 106, 281, 431, 683
BccI CCATC 2 cut(s) 529, 641
BceAI ACGGC 1 cut(s) 195
BcgI CGANNNNNNTGC 5 cut(s) 164, 198, 221, 255, 779
BciT130I CCWGG 2 cut(s) 615, 724
BciVI GTATCC 1 cut(s) 635
BcnI CCSGG 1 cut(s) 555
BcoDI GTCTC 1 cut(s) 371
BfaI CTAG 1 cut(s) 411
BfuI GTATCC 1 cut(s) 635
BisI GCNGC 5 cut(s) 27, 95, 270, 420, 672
BlsI GCNGC 5 cut(s) 28, 96, 271, 421, 673
Bme1390I CCNGG 3 cut(s) 555, 615, 724
Bme18I GGWCC 1 cut(s) 307
BmeRI GACNNNNNGTC 1 cut(s) 305
BmgT120I GGNCC 1 cut(s) 307
BmrFI CCNGG 3 cut(s) 555, 615, 724
BmsI GCATC 6 cut(s) 13, 53, 81, 241, 337, 745
Bpu14I TTCGAA 1 cut(s) 357
BpuEI CTTGAG 1 cut(s) 149
BpuMI CCSGG 1 cut(s) 555
Bsa29I ATCGAT 1 cut(s) 395
BsaJI CCNNGG 4 cut(s) 310, 504, 628, 723
Bsc4I CCNNNNNNNGG 3 cut(s) 38, 415, 724
Bse1I ACTGG 2 cut(s) 159, 718
BseBI CCWGG 2 cut(s) 615, 724
BseCI ATCGAT 1 cut(s) 395
BseDI CCNNGG 4 cut(s) 310, 504, 628, 723
BseGI GGATG 4 cut(s) 68, 232, 352, 760
BseLI CCNNNNNNNGG 3 cut(s) 38, 415, 724
BseMII CTCAG 1 cut(s) 110
BseNI ACTGG 2 cut(s) 159, 718
BseXI GCAGC 4 cut(s) 106, 281, 431, 683
Bsh1236I CGCG 1 cut(s) 29
Bsh1285I CGRYCG 1 cut(s) 678
BshVI ATCGAT 1 cut(s) 395
BsiEI CGRYCG 1 cut(s) 678
BsiSI CCGG 1 cut(s) 554
BslI CCNNNNNNNGG 3 cut(s) 38, 415, 724
BsmAI GTCTC 1 cut(s) 371
Bsp119I TTCGAA 1 cut(s) 357
Bsp1407I TGTACA 1 cut(s) 137
Bsp143I GATC 5 cut(s) 392, 396, 661, 688, 714
Bsp19I CCATGG 2 cut(s) 310, 628
BspACI CCGC 3 cut(s) 27, 517, 678
BspCNI CTCAG 1 cut(s) 111
BspDI ATCGAT 1 cut(s) 395
BspFNI CGCG 1 cut(s) 29
BspHI TCATGA 1 cut(s) 471
BspPI GGATC 2 cut(s) 400, 709
BspT104I TTCGAA 1 cut(s) 357
BsrBI CCGCTC 1 cut(s) 680
BsrGI TGTACA 1 cut(s) 137
BsrI ACTGG 2 cut(s) 159, 718
BssECI CCNNGG 4 cut(s) 310, 504, 628, 723
BssMI GATC 5 cut(s) 392, 396, 661, 688, 714
BssSI CACGAG 1 cut(s) 368
BssT1I CCWWGG 3 cut(s) 310, 504, 628
Bst2BI CACGAG 1 cut(s) 368
Bst2UI CCWGG 2 cut(s) 615, 724
Bst6I CTCTTC 1 cut(s) 322
BstAPI GCANNNNNTGC 1 cut(s) 229
BstAUI TGTACA 1 cut(s) 137
BstBI TTCGAA 1 cut(s) 357
BstC8I GCNNGC 2 cut(s) 208, 424
BstDEI CTNAG 2 cut(s) 119, 801
BstDSI CCRYGG 2 cut(s) 310, 628
BstF5I GGATG 4 cut(s) 68, 232, 352, 760
BstFNI CGCG 1 cut(s) 29
BstHHI GCGC 1 cut(s) 31
BstKTI GATC 5 cut(s) 395, 399, 664, 691, 717
BstMAI GTCTC 1 cut(s) 371
BstMBI GATC 5 cut(s) 392, 396, 661, 688, 714
BstMCI CGRYCG 1 cut(s) 678
BstMWI GCNNNNNNNGC 3 cut(s) 229, 432, 677
BstNI CCWGG 2 cut(s) 615, 724
BstNSI RCATGY 1 cut(s) 379
BstSCI CCNGG 3 cut(s) 553, 613, 722
BstUI CGCG 1 cut(s) 29
BstV1I GCAGC 4 cut(s) 106, 281, 431, 683
Bsu15I ATCGAT 1 cut(s) 395
BsuI GTATCC 1 cut(s) 635
BsuTUI ATCGAT 1 cut(s) 395
BtgI CCRYGG 2 cut(s) 310, 628
BtsCI GGATG 4 cut(s) 68, 232, 352, 760
BtsI GCAGTG 1 cut(s) 357
BtsIMutI CAGTG 2 cut(s) 287, 357
Cac8I GCNNGC 2 cut(s) 208, 424
CciI TCATGA 1 cut(s) 471
CfoI GCGC 1 cut(s) 31
Cfr13I GGNCC 1 cut(s) 307
ClaI ATCGAT 1 cut(s) 395
CseI GACGC 2 cut(s) 261, 599
Csp6I GTAC 1 cut(s) 138
CspCI CAANNNNNGTGG 2 cut(s) 351, 386
CviAII CATG 4 cut(s) 311, 376, 472, 629
CviJI RGCY 6 cut(s) 97, 258, 315, 422, 512, 730
CviKI_1 RGCY 6 cut(s) 97, 258, 315, 422, 512, 730
CviQI GTAC 1 cut(s) 138
DdeI CTNAG 2 cut(s) 119, 801
DpnI GATC 5 cut(s) 394, 398, 663, 690, 716
DpnII GATC 5 cut(s) 392, 396, 661, 688, 714
DriI GACNNNNNGTC 1 cut(s) 305
Eam1104I CTCTTC 1 cut(s) 322
Eam1105I GACNNNNNGTC 1 cut(s) 305
EarI CTCTTC 1 cut(s) 322
EciI GGCGGA 1 cut(s) 506
Eco130I CCWWGG 3 cut(s) 310, 504, 628
Eco47I GGWCC 1 cut(s) 307
Eco57I CTGAAG 1 cut(s) 248
EcoRII CCWGG 2 cut(s) 613, 722
EcoT14I CCWWGG 3 cut(s) 310, 504, 628
EcoT22I ATGCAT 1 cut(s) 68
ErhI CCWWGG 3 cut(s) 310, 504, 628
FaeI CATG 4 cut(s) 314, 379, 475, 632
FatI CATG 4 cut(s) 310, 375, 471, 628
FauNDI CATATG 1 cut(s) 19
Fnu4HI GCNGC 5 cut(s) 27, 95, 270, 420, 672
FokI GGATG 4 cut(s) 75, 219, 359, 767
Fsp4HI GCNGC 5 cut(s) 27, 95, 270, 420, 672
FspBI CTAG 1 cut(s) 411
GlaI GCGC 1 cut(s) 30
GluI GCNGC 5 cut(s) 27, 95, 270, 420, 672
HapII CCGG 1 cut(s) 554
HgaI GACGC 2 cut(s) 261, 599
HhaI GCGC 1 cut(s) 31
Hin1II CATG 4 cut(s) 314, 379, 475, 632
Hin6I GCGC 1 cut(s) 29
HinP1I GCGC 1 cut(s) 29
HincII GTYRAC 1 cut(s) 13
HindII GTYRAC 1 cut(s) 13
HinfI GANTC 1 cut(s) 580
HpaII CCGG 1 cut(s) 554
HphI GGTGA 1 cut(s) 398
Hpy166II GTNNAC 4 cut(s) 13, 138, 140, 373
Hpy188I TCNGA 1 cut(s) 346
Hpy188III TCNNGA 5 cut(s) 60, 166, 251, 472, 644
Hpy8I GTNNAC 4 cut(s) 13, 138, 140, 373
Hpy99I CGWCG 1 cut(s) 277
HpyAV CCTTC 1 cut(s) 290
HpyCH4IV ACGT 3 cut(s) 181, 301, 782
HpyCH4V TGCA 6 cut(s) 66, 94, 217, 232, 350, 426
HpyF10VI GCNNNNNNNGC 3 cut(s) 229, 432, 677
HpyF3I CTNAG 2 cut(s) 119, 801
HpySE526I ACGT 3 cut(s) 181, 301, 782
Hsp92II CATG 4 cut(s) 314, 379, 475, 632
HspAI GCGC 1 cut(s) 29
Kzo9I GATC 5 cut(s) 392, 396, 661, 688, 714
LmnI GCTCC 2 cut(s) 496, 727
Lsp1109I GCAGC 4 cut(s) 106, 281, 431, 683
LweI GCATC 6 cut(s) 13, 53, 81, 241, 337, 745
MaeI CTAG 1 cut(s) 411
MaeII ACGT 3 cut(s) 181, 301, 782
MaeIII GTNAC 3 cut(s) 244, 404, 632
MalI GATC 5 cut(s) 394, 398, 663, 690, 716
MbiI CCGCTC 1 cut(s) 680
MboI GATC 5 cut(s) 392, 396, 661, 688, 714
MboII GAAGA 1 cut(s) 339
MluCI AATT 6 cut(s) 113, 333, 544, 601, 657, 793
MnlI CCTC 5 cut(s) 184, 213, 277, 323, 502
Mph1103I ATGCAT 1 cut(s) 68
MseI TTAA 1 cut(s) 489
MslI CAYNNNNRTG 1 cut(s) 315
MspI CCGG 1 cut(s) 554
MspR9I CCNGG 3 cut(s) 555, 615, 724
MvaI CCWGG 2 cut(s) 615, 724
MvnI CGCG 1 cut(s) 29
MwoI GCNNNNNNNGC 3 cut(s) 229, 432, 677
NciI CCSGG 1 cut(s) 555
NcoI CCATGG 2 cut(s) 310, 628
NdeI CATATG 1 cut(s) 19
NdeII GATC 5 cut(s) 392, 396, 661, 688, 714
NlaIII CATG 4 cut(s) 314, 379, 475, 632
NmuCI GTSAC 2 cut(s) 244, 404
NsiI ATGCAT 1 cut(s) 68
NspI RCATGY 1 cut(s) 379
NspV TTCGAA 1 cut(s) 357
PagI TCATGA 1 cut(s) 471
PciI ACATGT 1 cut(s) 375
PfeI GAWTC 1 cut(s) 580
PflMI CCANNNNNTGG 1 cut(s) 38
PfoI TCCNGGA 1 cut(s) 613
PkrI GCNGC 5 cut(s) 28, 96, 271, 421, 673
PscI ACATGT 1 cut(s) 375
Psp6I CCWGG 2 cut(s) 613, 722
PspGI CCWGG 2 cut(s) 613, 722
PspPI GGNCC 1 cut(s) 307
RsaI GTAC 1 cut(s) 139
RsaNI GTAC 1 cut(s) 138
RseI CAYNNNNRTG 1 cut(s) 315
SaqAI TTAA 1 cut(s) 489
SatI GCNGC 5 cut(s) 27, 95, 270, 420, 672
Sau3AI GATC 5 cut(s) 392, 396, 661, 688, 714
Sau96I GGNCC 1 cut(s) 307
ScrFI CCNGG 3 cut(s) 555, 615, 724
SetI ASST 7 cut(s) 99, 184, 195, 260, 304, 732, 785
SfaNI GCATC 6 cut(s) 13, 53, 81, 241, 337, 745
SfuI TTCGAA 1 cut(s) 357
SinI GGWCC 1 cut(s) 307
SmiMI CAYNNNNRTG 1 cut(s) 315
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse9I AATT 6 cut(s) 113, 333, 544, 601, 657, 793
SsiI CCGC 3 cut(s) 27, 517, 678
SspMI CTAG 1 cut(s) 411
StyD4I CCNGG 3 cut(s) 553, 613, 722
StyI CCWWGG 3 cut(s) 310, 504, 628
TaiI ACGT 3 cut(s) 184, 304, 785
TaqI TCGA 6 cut(s) 107, 145, 357, 395, 643, 687
TaqII GACCGA 1 cut(s) 295
TasI AATT 6 cut(s) 113, 333, 544, 601, 657, 793
TatI WGTACW 1 cut(s) 137
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 1 cut(s) 580
Tru1I TTAA 1 cut(s) 489
Tru9I TTAA 1 cut(s) 489
TscAI CASTG 2 cut(s) 294, 357
TseFI GTSAC 2 cut(s) 244, 404
TseI GCWGC 4 cut(s) 94, 269, 419, 671
Tsp45I GTSAC 2 cut(s) 244, 404
TspDTI ATGAA 1 cut(s) 125
TspRI CASTG 2 cut(s) 294, 357
Van91I CCANNNNNTGG 1 cut(s) 38
VpaK11BI GGWCC 1 cut(s) 307
XapI RAATTY 1 cut(s) 544
XceI RCATGY 1 cut(s) 379
XspI CTAG 1 cut(s) 411
Zsp2I ATGCAT 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.