pycom111g05170

Domain of unknown function (DUF4336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0028444
Physical Location & Seq
Forward (+)
3328782 .. 3329643
862 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 390 bp
ATGGCAGCCATTGCCGTTTCTTCACCGAAATCCACCATTTTTCGGAACCCAATTTCTCTCAGAGAACCAAGTTCGAGCCCTTTTGGCGGGTCCTTGAAGGGTTTTTGTTTCCAGCTAAAACCCAGGAGCAAGAGCAGAAAGGCCGTCAATTTGGTGGTCTCTGCCCCAAACTGCACCATCAGTGGCAGCAGAGGCGGCGGTGACCGGTTTTACACCAACTTCACTGGGTTTCCTTTTCCTCTGGGTCCTTTTCCCAATAGAAGCACTACTAGGACTGAGGCTGTGAAAAGCTGCATATGGTTATTTGAACAAGAGCAAGCATTAGGGTTCAACAGTGTGTCAACAAACATTAAAATGACAGTCATCAAACTAAAATCCCGAGGATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4336

Protein Analysis

130

Amino Acids

14.01

Weight (kDa)

10.77

Isoelectric Point (pI)

59.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028444)

Species Orthologous Gene IDs
malus_domestica MD09G1061200.v1.1
pyrus_communis pycom111g05170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 87, 195, 198
AfiI CCNNNNNNNGG 2 cut(s) 42, 86
AgeI ACCGGT 1 cut(s) 204
AgsI TTSAA 3 cut(s) 97, 308, 331
AjnI CCWGG 1 cut(s) 122
AluBI AGCT 2 cut(s) 115, 291
AluI AGCT 2 cut(s) 115, 291
Alw26I GTCTC 1 cut(s) 163
Ama87I CYCGRG 1 cut(s) 378
AoxI GGCC 1 cut(s) 141
ApeKI GCWGC 3 cut(s) 5, 186, 291
AsiGI ACCGGT 1 cut(s) 204
AspS9I GGNCC 2 cut(s) 90, 245
AsuHPI GGTGA 2 cut(s) 15, 212
AvaI CYCGRG 1 cut(s) 378
AvaII GGWCC 2 cut(s) 90, 245
BanII GRGCYC 1 cut(s) 80
BbvI GCAGC 3 cut(s) 17, 198, 278
BccI CCATC 1 cut(s) 185
BceAI ACGGC 1 cut(s) 128
BciT130I CCWGG 1 cut(s) 124
BcoDI GTCTC 1 cut(s) 163
BfaI CTAG 1 cut(s) 270
BglI GCCNNNNNGGC 1 cut(s) 84
BisI GCNGC 4 cut(s) 6, 187, 196, 292
BlsI GCNGC 4 cut(s) 7, 188, 197, 293
Bme1390I CCNGG 1 cut(s) 124
Bme18I GGWCC 2 cut(s) 90, 245
BmeT110I CYCGRG 1 cut(s) 378
BmgT120I GGNCC 2 cut(s) 90, 245
BmiI GGNNCC 3 cut(s) 47, 91, 246
BmrFI CCNGG 1 cut(s) 124
BmrI ACTGGG 1 cut(s) 234
BmuI ACTGGG 1 cut(s) 234
BsaI GGTCTC 1 cut(s) 163
BsaJI CCNNGG 2 cut(s) 122, 379
BsaWI WCCGGW 1 cut(s) 204
Bsc4I CCNNNNNNNGG 2 cut(s) 42, 86
Bse118I RCCGGY 1 cut(s) 204
Bse1I ACTGG 1 cut(s) 229
Bse3DI GCAATG 1 cut(s) 9
BseBI CCWGG 1 cut(s) 124
BseDI CCNNGG 2 cut(s) 122, 379
BseLI CCNNNNNNNGG 2 cut(s) 42, 86
BseMI GCAATG 1 cut(s) 9
BseMII CTCAG 2 cut(s) 73, 267
BseNI ACTGG 1 cut(s) 229
BseXI GCAGC 3 cut(s) 17, 198, 278
BsgI GTGCAG 1 cut(s) 157
BshFI GGCC 1 cut(s) 143
BshTI ACCGGT 1 cut(s) 204
BsiHKCI CYCGRG 1 cut(s) 378
BsiSI CCGG 1 cut(s) 205
BslI CCNNNNNNNGG 2 cut(s) 42, 86
BsmAI GTCTC 1 cut(s) 163
BsnI GGCC 1 cut(s) 143
Bso31I GGTCTC 1 cut(s) 163
BsoBI CYCGRG 1 cut(s) 378
Bsp1286I GDGCHC 1 cut(s) 80
BspACI CCGC 3 cut(s) 87, 195, 198
BspANI GGCC 1 cut(s) 143
BspCNI CTCAG 2 cut(s) 72, 268
BspLI GGNNCC 3 cut(s) 47, 91, 246
BspTNI GGTCTC 1 cut(s) 163
BsrDI GCAATG 1 cut(s) 9
BsrFI RCCGGY 1 cut(s) 204
BsrI ACTGG 1 cut(s) 229
BssAI RCCGGY 1 cut(s) 204
BssECI CCNNGG 2 cut(s) 122, 379
Bst2UI CCWGG 1 cut(s) 124
Bst4CI ACNGT 2 cut(s) 335, 361
BstAPI GCANNNNNTGC 1 cut(s) 11
BstC8I GCNNGC 1 cut(s) 318
BstDEI CTNAG 2 cut(s) 59, 276
BstEII GGTNACC 1 cut(s) 200
BstMAI GTCTC 1 cut(s) 163
BstMWI GCNNNNNNNGC 4 cut(s) 11, 84, 192, 195
BstNI CCWGG 1 cut(s) 124
BstPI GGTNACC 1 cut(s) 200
BstSCI CCNGG 1 cut(s) 122
BstV1I GCAGC 3 cut(s) 17, 198, 278
BsuRI GGCC 1 cut(s) 143
BtsIMutI CAGTG 3 cut(s) 187, 222, 340
Cac8I GCNNGC 1 cut(s) 318
Cfr10I RCCGGY 1 cut(s) 204
Cfr13I GGNCC 2 cut(s) 90, 245
CspAI ACCGGT 1 cut(s) 204
CviJI RGCY 6 cut(s) 8, 78, 115, 143, 281, 291
CviKI_1 RGCY 6 cut(s) 8, 78, 115, 143, 281, 291
DdeI CTNAG 2 cut(s) 59, 276
Eco24I GRGCYC 1 cut(s) 80
Eco31I GGTCTC 1 cut(s) 163
Eco47I GGWCC 2 cut(s) 90, 245
Eco88I CYCGRG 1 cut(s) 378
Eco91I GGTNACC 1 cut(s) 200
EcoO109I RGGNCCY 2 cut(s) 90, 245
EcoO65I GGTNACC 1 cut(s) 200
EcoRII CCWGG 1 cut(s) 122
EcoT38I GRGCYC 1 cut(s) 80
FaiI YATR 2 cut(s) 296, 298
FauI CCCGC 1 cut(s) 80
FauNDI CATATG 1 cut(s) 296
Fnu4HI GCNGC 4 cut(s) 6, 187, 196, 292
FriOI GRGCYC 1 cut(s) 80
Fsp4HI GCNGC 4 cut(s) 6, 187, 196, 292
FspBI CTAG 1 cut(s) 270
GluI GCNGC 4 cut(s) 6, 187, 196, 292
HaeIII GGCC 1 cut(s) 143
HapII CCGG 1 cut(s) 205
HincII GTYRAC 1 cut(s) 342
HindII GTYRAC 1 cut(s) 342
HpaII CCGG 1 cut(s) 205
HphI GGTGA 2 cut(s) 15, 212
Hpy166II GTNNAC 1 cut(s) 342
Hpy188I TCNGA 2 cut(s) 45, 62
Hpy188III TCNNGA 1 cut(s) 378
Hpy8I GTNNAC 1 cut(s) 342
HpyAV CCTTC 1 cut(s) 91
HpyCH4III ACNGT 2 cut(s) 335, 361
HpyCH4V TGCA 2 cut(s) 174, 294
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 84, 192, 195
HpyF3I CTNAG 2 cut(s) 59, 276
LmnI GCTCC 1 cut(s) 126
LpnPI CCDG 6 cut(s) 109, 125, 136, 210, 218, 227
Lsp1109I GCAGC 3 cut(s) 17, 198, 278
MaeI CTAG 1 cut(s) 270
MaeIII GTNAC 1 cut(s) 200
MboII GAAGA 1 cut(s) 12
MhlI GDGCHC 1 cut(s) 80
MluCI AATT 2 cut(s) 51, 148
MnlI CCTC 4 cut(s) 185, 249, 271, 374
MseI TTAA 1 cut(s) 351
MslI CAYNNNNRTG 1 cut(s) 353
MspI CCGG 1 cut(s) 205
MspR9I CCNGG 1 cut(s) 124
MvaI CCWGG 1 cut(s) 124
MwoI GCNNNNNNNGC 4 cut(s) 11, 84, 192, 195
NdeI CATATG 1 cut(s) 296
NlaIV GGNNCC 3 cut(s) 47, 91, 246
NmuCI GTSAC 1 cut(s) 200
PinAI ACCGGT 1 cut(s) 204
PkrI GCNGC 4 cut(s) 7, 188, 197, 293
PpuMI RGGWCCY 2 cut(s) 90, 245
Psp5II RGGWCCY 2 cut(s) 90, 245
Psp6I CCWGG 1 cut(s) 122
PspEI GGTNACC 1 cut(s) 200
PspGI CCWGG 1 cut(s) 122
PspN4I GGNNCC 3 cut(s) 47, 91, 246
PspPI GGNCC 2 cut(s) 90, 245
PspPPI RGGWCCY 2 cut(s) 90, 245
RseI CAYNNNNRTG 1 cut(s) 353
SaqAI TTAA 1 cut(s) 351
SatI GCNGC 4 cut(s) 6, 187, 196, 292
Sau96I GGNCC 2 cut(s) 90, 245
ScrFI CCNGG 1 cut(s) 124
SduI GDGCHC 1 cut(s) 80
SetI ASST 2 cut(s) 117, 293
SinI GGWCC 2 cut(s) 90, 245
SmiMI CAYNNNNRTG 1 cut(s) 353
Sse9I AATT 2 cut(s) 51, 148
SsiI CCGC 3 cut(s) 87, 195, 198
SspMI CTAG 1 cut(s) 270
StyD4I CCNGG 1 cut(s) 122
TaaI ACNGT 2 cut(s) 335, 361
TaqI TCGA 1 cut(s) 74
TasI AATT 2 cut(s) 51, 148
TauI GCSGC 1 cut(s) 198
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TscAI CASTG 3 cut(s) 187, 229, 340
TseFI GTSAC 1 cut(s) 200
TseI GCWGC 3 cut(s) 5, 186, 291
Tsp45I GTSAC 1 cut(s) 200
TspRI CASTG 3 cut(s) 187, 229, 340
VpaK11BI GGWCC 2 cut(s) 90, 245
XspI CTAG 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.