pycom11g03640

KH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0028496
Physical Location & Seq
Forward (+)
2820566 .. 2823970
3405 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 540 bp
ATGAGAGAGAGAGAGCGTCTGGTTGGGAATCGCGATCGTCCCACCTGCCAGCCTCAGACCTACAGAGGGAGAGTCACCTCCGACGACCAGAAGAAAGAGAAGAAGACGATCGGACGGCTGAGAGGCGCTATGGAAATGATGTCATCATCGGGCGGTGGAAGGTACATGGCTTACTCGCGTTCACCGTCGGCACCTCACTCCCCTCACCTCTCCGGCCTTCGCTCCGCCGCTCTCGTCGCCGCCGAGCAAGAAAAATATCTATTGGAGCTGCTTGGGGAGCGTCACAAGCTGAGTCCATTTTTGCTAGTTCTGCCGAATTGCAACAGGTTACTAAACCAAGAAATATTGCGTGTAACTACACTGTTGGGGAATGCGTCAGTTTTAGGTCAAAGTGGGCTTGAACATGCTAGCCCCCTGGCATCTAGAGGAATGTTCTCAAATGGAGGTGCTGATGTGAACGGATGGGCATCACGTTTTCAATCAGAAGTCAGGTTTATTACAGCCTTCATCAACCCAAAACTGGCTGAGTCCTCAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_1 STAR_dimer

Protein Analysis

180

Amino Acids

19.76

Weight (kDa)

10.13

Isoelectric Point (pI)

52.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
STAR_dimer PF16544 83 - 122 4.5e-09 Homodimerisation region of STAR domain protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0028496)

Species Orthologous Gene IDs
malus_domestica MD03G1041200.v1.1
pyrus_communis pycom11g03640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 53
Acc36I ACCTGC 1 cut(s) 53
AccB1I GGYRCC 1 cut(s) 190
AccBSI CCGCTC 1 cut(s) 230
AccII CGCG 2 cut(s) 33, 178
AciI CCGC 4 cut(s) 153, 225, 228, 240
AfaI GTAC 1 cut(s) 164
AfiI CCNNNNNNNGG 2 cut(s) 66, 520
AgsI TTSAA 2 cut(s) 401, 479
AjnI CCWGG 1 cut(s) 414
AluBI AGCT 2 cut(s) 268, 289
AluI AGCT 2 cut(s) 268, 289
AoxI GGCC 1 cut(s) 214
ApeKI GCWGC 1 cut(s) 268
AspLEI GCGC 1 cut(s) 128
AsuHPI GGTGA 3 cut(s) 67, 174, 197
AsuNHI GCTAGC 1 cut(s) 407
BanI GGYRCC 1 cut(s) 190
BbsI GAAGAC 1 cut(s) 110
BbvI GCAGC 1 cut(s) 255
BccI CCATC 1 cut(s) 456
BceAI ACGGC 1 cut(s) 131
BciT130I CCWGG 1 cut(s) 416
BfaI CTAG 3 cut(s) 305, 408, 423
BfmI CTRYAG 1 cut(s) 61
BfoI RGCGCY 1 cut(s) 129
BfuAI ACCTGC 1 cut(s) 53
BisI GCNGC 3 cut(s) 228, 240, 269
BlsI GCNGC 3 cut(s) 229, 241, 270
Bme1390I CCNGG 1 cut(s) 416
BmiI GGNNCC 1 cut(s) 192
BmrFI CCNGG 1 cut(s) 416
BmsI GCATC 2 cut(s) 428, 476
BmtI GCTAGC 1 cut(s) 411
BpiI GAAGAC 1 cut(s) 110
BsaBI GATNNNNATC 1 cut(s) 466
BsaJI CCNNGG 1 cut(s) 414
Bsc4I CCNNNNNNNGG 2 cut(s) 66, 520
Bse1I ACTGG 1 cut(s) 525
Bse8I GATNNNNATC 1 cut(s) 466
BseBI CCWGG 1 cut(s) 416
BseDI CCNNGG 1 cut(s) 414
BseGI GGATG 1 cut(s) 467
BseJI GATNNNNATC 1 cut(s) 466
BseLI CCNNNNNNNGG 2 cut(s) 66, 520
BseMII CTCAG 4 cut(s) 68, 110, 281, 516
BseNI ACTGG 1 cut(s) 525
BseXI GCAGC 1 cut(s) 255
Bsh1236I CGCG 2 cut(s) 33, 178
Bsh1285I CGRYCG 2 cut(s) 37, 111
BshFI GGCC 1 cut(s) 216
BshNI GGYRCC 1 cut(s) 190
BsiEI CGRYCG 2 cut(s) 37, 111
BsiSI CCGG 1 cut(s) 213
BslFI GGGAC 1 cut(s) 24
BslI CCNNNNNNNGG 2 cut(s) 66, 520
BsmFI GGGAC 1 cut(s) 24
BsmI GAATGC 1 cut(s) 376
BsnI GGCC 1 cut(s) 216
Bsp143I GATC 2 cut(s) 34, 108
Bsp68I TCGCGA 1 cut(s) 33
BspACI CCGC 4 cut(s) 153, 225, 228, 240
BspANI GGCC 1 cut(s) 216
BspCNI CTCAG 4 cut(s) 67, 111, 282, 517
BspFNI CGCG 2 cut(s) 33, 178
BspLI GGNNCC 1 cut(s) 192
BspMI ACCTGC 1 cut(s) 53
BspOI GCTAGC 1 cut(s) 411
BspT107I GGYRCC 1 cut(s) 190
BsrBI CCGCTC 1 cut(s) 230
BsrI ACTGG 1 cut(s) 525
BssECI CCNNGG 1 cut(s) 414
BssMI GATC 2 cut(s) 34, 108
Bst2UI CCWGG 1 cut(s) 416
Bst4CI ACNGT 2 cut(s) 186, 363
BstC8I GCNNGC 2 cut(s) 50, 409
BstDEI CTNAG 4 cut(s) 54, 119, 290, 525
BstF5I GGATG 1 cut(s) 467
BstFNI CGCG 2 cut(s) 33, 178
BstH2I RGCGCY 1 cut(s) 129
BstHHI GCGC 1 cut(s) 128
BstKTI GATC 2 cut(s) 37, 111
BstMBI GATC 2 cut(s) 34, 108
BstMCI CGRYCG 2 cut(s) 37, 111
BstMWI GCNNNNNNNGC 4 cut(s) 236, 277, 286, 310
BstNI CCWGG 1 cut(s) 416
BstNSI RCATGY 1 cut(s) 407
BstSCI CCNGG 1 cut(s) 414
BstSFI CTRYAG 1 cut(s) 61
BstUI CGCG 2 cut(s) 33, 178
BstV1I GCAGC 1 cut(s) 255
BstV2I GAAGAC 1 cut(s) 110
BsuRI GGCC 1 cut(s) 216
BtsCI GGATG 1 cut(s) 467
BtsIMutI CAGTG 1 cut(s) 359
BtuMI TCGCGA 1 cut(s) 33
BveI ACCTGC 1 cut(s) 53
Cac8I GCNNGC 2 cut(s) 50, 409
CfoI GCGC 1 cut(s) 128
CseI GACGC 3 cut(s) 5, 269, 363
Csp6I GTAC 1 cut(s) 163
CviAII CATG 2 cut(s) 166, 404
CviQI GTAC 1 cut(s) 163
DdeI CTNAG 4 cut(s) 54, 119, 290, 525
DpnI GATC 2 cut(s) 36, 110
DpnII GATC 2 cut(s) 34, 108
EciI GGCGGA 1 cut(s) 214
EcoRII CCWGG 1 cut(s) 414
FaeI CATG 2 cut(s) 169, 407
FaiI YATR 3 cut(s) 131, 167, 405
FaqI GGGAC 1 cut(s) 24
FatI CATG 2 cut(s) 165, 403
Fnu4HI GCNGC 3 cut(s) 228, 240, 269
FokI GGATG 1 cut(s) 474
Fsp4HI GCNGC 3 cut(s) 228, 240, 269
FspBI CTAG 3 cut(s) 305, 408, 423
GlaI GCGC 1 cut(s) 127
GluI GCNGC 3 cut(s) 228, 240, 269
HaeII RGCGCY 1 cut(s) 129
HaeIII GGCC 1 cut(s) 216
HapII CCGG 1 cut(s) 213
HgaI GACGC 3 cut(s) 5, 269, 363
HhaI GCGC 1 cut(s) 128
Hin1II CATG 2 cut(s) 169, 407
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HinfI GANTC 4 cut(s) 28, 72, 292, 527
HpaII CCGG 1 cut(s) 213
HphI GGTGA 3 cut(s) 67, 174, 197
Hpy166II GTNNAC 2 cut(s) 182, 457
Hpy188I TCNGA 4 cut(s) 57, 82, 113, 484
Hpy188III TCNNGA 2 cut(s) 32, 423
Hpy8I GTNNAC 2 cut(s) 182, 457
Hpy99I CGWCG 3 cut(s) 86, 190, 239
HpyAV CCTTC 3 cut(s) 153, 227, 514
HpyCH4III ACNGT 2 cut(s) 186, 363
HpyCH4IV ACGT 1 cut(s) 472
HpyCH4V TGCA 1 cut(s) 321
HpyF10VI GCNNNNNNNGC 4 cut(s) 236, 277, 286, 310
HpyF3I CTNAG 4 cut(s) 54, 119, 290, 525
HpySE526I ACGT 1 cut(s) 472
Hsp92II CATG 2 cut(s) 169, 407
HspAI GCGC 1 cut(s) 126
Kzo9I GATC 2 cut(s) 34, 108
LmnI GCTCC 3 cut(s) 227, 265, 277
Lsp1109I GCAGC 1 cut(s) 255
LweI GCATC 2 cut(s) 428, 476
MaeI CTAG 3 cut(s) 305, 408, 423
MaeII ACGT 1 cut(s) 472
MaeIII GTNAC 4 cut(s) 73, 281, 327, 352
MalI GATC 2 cut(s) 36, 110
MbiI CCGCTC 1 cut(s) 230
MboI GATC 2 cut(s) 34, 108
MboII GAAGA 3 cut(s) 103, 112, 115
MluCI AATT 1 cut(s) 316
MlyI GAGTC 3 cut(s) 81, 301, 536
MmeI TCCRAC 1 cut(s) 105
MnlI CCTC 9 cut(s) 59, 63, 88, 116, 204, 213, 218, 419, 437
MspI CCGG 1 cut(s) 213
MspR9I CCNGG 1 cut(s) 416
Mva1269I GAATGC 1 cut(s) 376
MvaI CCWGG 1 cut(s) 416
MvnI CGCG 2 cut(s) 33, 178
MwoI GCNNNNNNNGC 4 cut(s) 236, 277, 286, 310
NdeII GATC 2 cut(s) 34, 108
NheI GCTAGC 1 cut(s) 407
NlaIII CATG 2 cut(s) 169, 407
NlaIV GGNNCC 1 cut(s) 192
NmeAIII GCCGAG 1 cut(s) 268
NmuCI GTSAC 2 cut(s) 73, 281
NruI TCGCGA 1 cut(s) 33
NspI RCATGY 1 cut(s) 407
PaqCI CACCTGC 1 cut(s) 53
PcsI WCGNNNNNNNCGW 2 cut(s) 182, 240
PctI GAATGC 1 cut(s) 376
PfeI GAWTC 1 cut(s) 28
PkrI GCNGC 3 cut(s) 229, 241, 270
Ple19I CGATCG 2 cut(s) 37, 111
PleI GAGTC 3 cut(s) 80, 300, 535
PpsI GAGTC 3 cut(s) 80, 300, 535
Psp6I CCWGG 1 cut(s) 414
PspGI CCWGG 1 cut(s) 414
PspN4I GGNNCC 1 cut(s) 192
PvuI CGATCG 2 cut(s) 37, 111
RruI TCGCGA 1 cut(s) 33
RsaI GTAC 1 cut(s) 164
RsaNI GTAC 1 cut(s) 163
SatI GCNGC 3 cut(s) 228, 240, 269
Sau3AI GATC 2 cut(s) 34, 108
SchI GAGTC 3 cut(s) 81, 301, 536
ScrFI CCNGG 1 cut(s) 416
SfaNI GCATC 2 cut(s) 428, 476
SfcI CTRYAG 1 cut(s) 61
Sse9I AATT 1 cut(s) 316
SsiI CCGC 4 cut(s) 153, 225, 228, 240
SspI AATATT 1 cut(s) 345
SspMI CTAG 3 cut(s) 305, 408, 423
StyD4I CCNGG 1 cut(s) 414
TaaI ACNGT 2 cut(s) 186, 363
TaiI ACGT 1 cut(s) 475
TasI AATT 1 cut(s) 316
TauI GCSGC 2 cut(s) 230, 242
TfiI GAWTC 1 cut(s) 28
TscAI CASTG 1 cut(s) 366
TseFI GTSAC 2 cut(s) 73, 281
TseI GCWGC 1 cut(s) 268
Tsp45I GTSAC 2 cut(s) 73, 281
TspDTI ATGAA 1 cut(s) 496
TspGWI ACGGA 1 cut(s) 474
TspRI CASTG 1 cut(s) 366
XbaI TCTAGA 1 cut(s) 422
XceI RCATGY 1 cut(s) 407
XspI CTAG 3 cut(s) 305, 408, 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.