pycom11g06710

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0013864
Physical Location & Seq
Reverse (-)
5162646 .. 5163443
798 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 798 bp
ATGAACGACGCCGTCGCCGCCCGCAAGTTGACACCGTCCGACGACCTCATCTCCCGCTTCATGAACAAGCGCGACGTCGACGGCAACCCATTCCCAAGCGCCGTCCTCCAGCGCATCGCTCTTAACTTCGTCCTCGCCGGCCGCGACACGTCGTCGGTGGCGCTCAGCTGGTTCTTCCACCTCATCATGGAAAATCCCCACATCGAGGAGAAAATCATCGCCGAGATCTCGACGGTGCTGAAACAGACACGTGGTGATGATCCGCGGCTATGGACGGAGGAGCCCTTGGTGTTCGACGAGGCGGATAAATTGGTTTACTTGAAGGCGGCGCTGGCTGAGACTTTACGACTATACCCCTCCGTGCCGGAGGATTTCAAGTACGTCGTGAAAGACGACGTTCTGCCTGACGGGACTTTCGTGCCGGCCGGTTCGACGGTGACGTATTCGATTTACTCGGTGGGGAGGATGAAGAGCATTTGGGGGGAGGATTGCATGGAGTTTAAACCGGAACGGTGGATGTCGGCCGACGGGAACCGGTTCGAACCGCCCCAGGACGGTTACAAGTTCGTGGCTTTCAATGCTGGACCGAGGACTTGTTTGGGGAAGGACTTGGCTTACCTGCAGATGAAATCGGTGGTTTCTGCCGTGCTTTTGCGTTACCGGCTGTCTCCGGTTCCCGGTTTCCGAGCACAGCAGAAGATGTCGCTCACACTCTTCATGAAGAACGGGCTTCAGGTGCATTTGCGTCCACGTAAGCTTGGTGGTGGGCCAGGTTATGTGGCTGCCACCTCAGCATAG

Protein Analysis

266

Amino Acids

29.67

Weight (kDa)

8.41

Isoelectric Point (pI)

43.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 14 - 237 1.2e-48 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013864)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58860
fragaria_vesca FvH4_3g38270
malus_domestica MD03G1073600.v1.1 MD11G1079600.v1.1
prunus_persica Prupe.6G060200_v2.0.a1
pyrus_communis pycom03g05950 pycom11g06710
rosa_chinensis RchiOBHm_Chr5g0068771
rosa_laevigata RLG00000036009
rosa_multiflora Rmu_sc0000376.1_g000036
rosa_roxburghii Rroxscaffold_1G00012050
rosa_rugosa Rorug05G0393100
rosa_samantha Rh5AG450600 Rh5BG469100 Rh5CG491300 Rh5DG482400
rosa_wichuraiana Rw5G042060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 78
Acc36I ACCTGC 1 cut(s) 627
AccI GTMKAC 1 cut(s) 78
AccII CGCG 3 cut(s) 72, 144, 265
AciI CCGC 9 cut(s) 18, 22, 55, 142, 263, 265, 302, 326, 545
AclWI GGATC 1 cut(s) 254
AcoI YGGCCR 3 cut(s) 139, 423, 522
AcuI CTGAAG 1 cut(s) 716
AcvI CACGTG 1 cut(s) 251
AcyI GRCGYC 2 cut(s) 9, 75
AdeI CACNNNGTG 1 cut(s) 254
AfaI GTAC 1 cut(s) 380
AfiI CCNNNNNNNGG 4 cut(s) 187, 205, 554, 677
AflIII ACRYGT 2 cut(s) 147, 248
AgeI ACCGGT 1 cut(s) 534
AgsI TTSAA 3 cut(s) 322, 376, 577
AhdI GACNNNNNGTC 1 cut(s) 151
AjiI CACGTC 1 cut(s) 150
AjnI CCWGG 2 cut(s) 549, 769
AluBI AGCT 2 cut(s) 168, 757
AluI AGCT 2 cut(s) 168, 757
Alw21I GWGCWC 1 cut(s) 691
Alw26I GTCTC 2 cut(s) 332, 672
AlwI GGATC 1 cut(s) 254
AoxI GGCC 4 cut(s) 139, 423, 522, 767
ApeKI GCWGC 1 cut(s) 782
ArsI GACNNNNNNTTYG 2 cut(s) 398, 430
AsiGI ACCGGT 1 cut(s) 534
Asp700I GAANNNNTTC 1 cut(s) 536
AspLEI GCGC 5 cut(s) 72, 101, 114, 163, 331
AspS9I GGNCC 2 cut(s) 584, 767
AsuC2I CCSGG 1 cut(s) 678
AsuHPI GGTGA 2 cut(s) 266, 448
AsuII TTCGAA 1 cut(s) 540
AvaII GGWCC 1 cut(s) 584
BanII GRGCYC 1 cut(s) 285
BbrPI CACGTG 1 cut(s) 251
Bbv12I GWGCWC 1 cut(s) 691
BbvCI CCTCAGC 1 cut(s) 790
BbvI GCAGC 1 cut(s) 769
BceAI ACGGC 3 cut(s) 86, 97, 629
BciT130I CCWGG 2 cut(s) 551, 771
BcnI CCSGG 1 cut(s) 678
BcoDI GTCTC 2 cut(s) 332, 672
BfmI CTRYAG 1 cut(s) 620
BfoI RGCGCY 3 cut(s) 102, 164, 332
BfuAI ACCTGC 1 cut(s) 627
BglII AGATCT 1 cut(s) 225
BisI GCNGC 5 cut(s) 18, 142, 266, 327, 783
BlpI GCTNAGC 1 cut(s) 164
BlsI GCNGC 5 cut(s) 19, 143, 267, 328, 784
Bme1390I CCNGG 3 cut(s) 551, 678, 771
Bme18I GGWCC 1 cut(s) 584
BmeRI GACNNNNNGTC 1 cut(s) 151
BmgBI CACGTC 1 cut(s) 150
BmgT120I GGNCC 2 cut(s) 584, 767
BmiI GGNNCC 3 cut(s) 282, 533, 675
BmrFI CCNGG 3 cut(s) 551, 678, 771
BmsI GCATC 1 cut(s) 123
BpmI CTGGAG 1 cut(s) 92
Bpu10I CCTNAGC 1 cut(s) 790
Bpu1102I GCTNAGC 1 cut(s) 164
Bpu14I TTCGAA 1 cut(s) 540
BpuMI CCSGG 1 cut(s) 678
BsaAI YACGTR 2 cut(s) 251, 752
BsaHI GRCGYC 2 cut(s) 9, 75
BsaJI CCNNGG 4 cut(s) 263, 285, 549, 587
BsaWI WCCGGW 3 cut(s) 505, 534, 670
BsaXI ACNNNNNCTCC 2 cut(s) 35, 65
Bsc4I CCNNNNNNNGG 4 cut(s) 187, 205, 554, 677
Bse118I RCCGGY 5 cut(s) 137, 421, 425, 534, 660
BseBI CCWGG 2 cut(s) 551, 771
BseDI CCNNGG 4 cut(s) 263, 285, 549, 587
BseGI GGATG 2 cut(s) 471, 522
BseLI CCNNNNNNNGG 4 cut(s) 187, 205, 554, 677
BseMII CTCAG 2 cut(s) 178, 327
BseRI GAGGAG 2 cut(s) 221, 293
BseX3I CGGCCG 3 cut(s) 139, 423, 522
BseXI GCAGC 1 cut(s) 769
Bsh1236I CGCG 3 cut(s) 72, 144, 265
Bsh1285I CGRYCG 3 cut(s) 142, 426, 525
BshFI GGCC 4 cut(s) 141, 425, 524, 769
BshTI ACCGGT 1 cut(s) 534
BsiEI CGRYCG 3 cut(s) 142, 426, 525
BsiHKAI GWGCWC 1 cut(s) 691
BsiSI CCGG 9 cut(s) 138, 365, 422, 426, 506, 535, 661, 671, 678
BslFI GGGAC 1 cut(s) 424
BslI CCNNNNNNNGG 4 cut(s) 187, 205, 554, 677
BsmAI GTCTC 2 cut(s) 332, 672
BsmFI GGGAC 1 cut(s) 424
BsnI GGCC 4 cut(s) 141, 425, 524, 769
Bsp119I TTCGAA 1 cut(s) 540
Bsp1286I GDGCHC 2 cut(s) 285, 691
Bsp143I GATC 2 cut(s) 225, 259
Bsp1720I GCTNAGC 1 cut(s) 164
BspACI CCGC 9 cut(s) 18, 22, 55, 142, 263, 265, 302, 326, 545
BspANI GGCC 4 cut(s) 141, 425, 524, 769
BspCNI CTCAG 2 cut(s) 177, 328
BspFNI CGCG 3 cut(s) 72, 144, 265
BspHI TCATGA 2 cut(s) 60, 717
BspLI GGNNCC 3 cut(s) 282, 533, 675
BspMAI CTGCAG 1 cut(s) 624
BspMI ACCTGC 1 cut(s) 627
BspPI GGATC 1 cut(s) 254
BspQI GCTCTTC 1 cut(s) 464
BspT104I TTCGAA 1 cut(s) 540
BsrFI RCCGGY 5 cut(s) 137, 421, 425, 534, 660
BssAI RCCGGY 5 cut(s) 137, 421, 425, 534, 660
BssECI CCNNGG 4 cut(s) 263, 285, 549, 587
BssMI GATC 2 cut(s) 225, 259
BssNI GRCGYC 2 cut(s) 9, 75
BssT1I CCWWGG 1 cut(s) 285
Bst2UI CCWGG 2 cut(s) 551, 771
Bst4CI ACNGT 5 cut(s) 36, 235, 436, 513, 557
Bst6I CTCTTC 2 cut(s) 464, 719
BstACI GRCGYC 2 cut(s) 9, 75
BstBAI YACGTR 2 cut(s) 251, 752
BstBI TTCGAA 1 cut(s) 540
BstC8I GCNNGC 4 cut(s) 22, 139, 333, 423
BstDEI CTNAG 3 cut(s) 164, 336, 790
BstDSI CCRYGG 1 cut(s) 263
BstF5I GGATG 2 cut(s) 471, 522
BstFNI CGCG 3 cut(s) 72, 144, 265
BstH2I RGCGCY 3 cut(s) 102, 164, 332
BstHHI GCGC 5 cut(s) 72, 101, 114, 163, 331
BstKTI GATC 2 cut(s) 228, 262
BstMAI GTCTC 2 cut(s) 332, 672
BstMBI GATC 2 cut(s) 225, 259
BstMCI CGRYCG 3 cut(s) 142, 426, 525
BstMWI GCNNNNNNNGC 6 cut(s) 17, 332, 578, 661, 736, 791
BstNI CCWGG 2 cut(s) 551, 771
BstSCI CCNGG 3 cut(s) 549, 676, 769
BstSFI CTRYAG 1 cut(s) 620
BstUI CGCG 3 cut(s) 72, 144, 265
BstV1I GCAGC 1 cut(s) 769
BstX2I RGATCY 1 cut(s) 225
BstYI RGATCY 1 cut(s) 225
BstZI CGGCCG 3 cut(s) 139, 423, 522
BsuRI GGCC 4 cut(s) 141, 425, 524, 769
BtgI CCRYGG 1 cut(s) 263
BtgZI GCGATG 2 cut(s) 100, 202
BtrI CACGTC 1 cut(s) 150
BtsCI GGATG 2 cut(s) 471, 522
BveI ACCTGC 1 cut(s) 627
Cac8I GCNNGC 4 cut(s) 22, 139, 333, 423
CciI TCATGA 2 cut(s) 60, 717
CfoI GCGC 5 cut(s) 72, 101, 114, 163, 331
Cfr10I RCCGGY 5 cut(s) 137, 421, 425, 534, 660
Cfr13I GGNCC 2 cut(s) 584, 767
Cfr42I CCGCGG 1 cut(s) 266
CseI GACGC 2 cut(s) 17, 734
Csp6I GTAC 1 cut(s) 379
CspAI ACCGGT 1 cut(s) 534
CviAII CATG 4 cut(s) 61, 187, 493, 718
CviQI GTAC 1 cut(s) 379
DdeI CTNAG 3 cut(s) 164, 336, 790
DpnI GATC 2 cut(s) 227, 261
DpnII GATC 2 cut(s) 225, 259
DraI TTTAAA 1 cut(s) 502
DraIII CACNNNGTG 1 cut(s) 254
DriI GACNNNNNGTC 1 cut(s) 151
EaeI YGGCCR 3 cut(s) 139, 423, 522
EagI CGGCCG 3 cut(s) 139, 423, 522
Eam1104I CTCTTC 2 cut(s) 464, 719
Eam1105I GACNNNNNGTC 1 cut(s) 151
EarI CTCTTC 2 cut(s) 464, 719
EciI GGCGGA 1 cut(s) 317
EclXI CGGCCG 3 cut(s) 139, 423, 522
Eco130I CCWWGG 1 cut(s) 285
Eco24I GRGCYC 1 cut(s) 285
Eco47I GGWCC 1 cut(s) 584
Eco52I CGGCCG 3 cut(s) 139, 423, 522
Eco57I CTGAAG 1 cut(s) 716
Eco72I CACGTG 1 cut(s) 251
EcoRII CCWGG 2 cut(s) 549, 769
EcoT14I CCWWGG 1 cut(s) 285
EcoT38I GRGCYC 1 cut(s) 285
ErhI CCWWGG 1 cut(s) 285
FaeI CATG 4 cut(s) 64, 190, 496, 721
FaiI YATR 8 cut(s) 62, 188, 271, 352, 494, 719, 777, 796
FaqI GGGAC 1 cut(s) 424
FatI CATG 4 cut(s) 60, 186, 492, 717
FauI CCCGC 2 cut(s) 29, 62
FblI GTMKAC 1 cut(s) 78
Fnu4HI GCNGC 5 cut(s) 18, 142, 266, 327, 783
FokI GGATG 2 cut(s) 478, 529
FriOI GRGCYC 1 cut(s) 285
Fsp4HI GCNGC 5 cut(s) 18, 142, 266, 327, 783
GlaI GCGC 5 cut(s) 71, 100, 113, 162, 330
GluI GCNGC 5 cut(s) 18, 142, 266, 327, 783
GsuI CTGGAG 1 cut(s) 92
HaeII RGCGCY 3 cut(s) 102, 164, 332
HaeIII GGCC 4 cut(s) 141, 425, 524, 769
HapII CCGG 9 cut(s) 138, 365, 422, 426, 506, 535, 661, 671, 678
HgaI GACGC 2 cut(s) 17, 734
HhaI GCGC 5 cut(s) 72, 101, 114, 163, 331
Hin1I GRCGYC 2 cut(s) 9, 75
Hin1II CATG 4 cut(s) 64, 190, 496, 721
Hin6I GCGC 5 cut(s) 70, 99, 112, 161, 329
HinP1I GCGC 5 cut(s) 70, 99, 112, 161, 329
HincII GTYRAC 2 cut(s) 30, 79
HindII GTYRAC 2 cut(s) 30, 79
HindIII AAGCTT 1 cut(s) 755
HpaII CCGG 9 cut(s) 138, 365, 422, 426, 506, 535, 661, 671, 678
HphI GGTGA 2 cut(s) 266, 448
Hpy166II GTNNAC 4 cut(s) 30, 79, 316, 749
Hpy188I TCNGA 2 cut(s) 40, 686
Hpy188III TCNNGA 4 cut(s) 61, 229, 385, 718
Hpy8I GTNNAC 4 cut(s) 30, 79, 316, 749
HpyAV CCTTC 2 cut(s) 316, 598
HpyCH4III ACNGT 5 cut(s) 36, 235, 436, 513, 557
HpyCH4IV ACGT 7 cut(s) 75, 149, 250, 381, 396, 440, 751
HpyCH4V TGCA 3 cut(s) 492, 622, 739
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 332, 578, 661, 736, 791
HpyF3I CTNAG 3 cut(s) 164, 336, 790
HpySE526I ACGT 7 cut(s) 75, 149, 250, 381, 396, 440, 751
Hsp92I GRCGYC 2 cut(s) 9, 75
Hsp92II CATG 4 cut(s) 64, 190, 496, 721
HspAI GCGC 5 cut(s) 70, 99, 112, 161, 329
KroI GCCGGC 2 cut(s) 137, 421
KroNI GCCGGC 2 cut(s) 139, 423
KspI CCGCGG 1 cut(s) 266
Kzo9I GATC 2 cut(s) 225, 259
LguI GCTCTTC 1 cut(s) 464
LmnI GCTCC 1 cut(s) 280
Lsp1109I GCAGC 1 cut(s) 769
LweI GCATC 1 cut(s) 123
MaeII ACGT 7 cut(s) 75, 149, 250, 381, 396, 440, 751
MaeIII GTNAC 3 cut(s) 436, 557, 656
MalI GATC 2 cut(s) 227, 261
MboI GATC 2 cut(s) 225, 259
MboII GAAGA 5 cut(s) 166, 481, 706, 709, 733
MflI RGATCY 1 cut(s) 225
MhlI GDGCHC 2 cut(s) 285, 691
MluCI AATT 1 cut(s) 308
MmeI TCCRAC 1 cut(s) 63
MroNI GCCGGC 2 cut(s) 137, 421
MroXI GAANNNNTTC 1 cut(s) 536
MseI TTAA 2 cut(s) 123, 501
MspA1I CMGCKG 2 cut(s) 168, 265
MspI CCGG 9 cut(s) 138, 365, 422, 426, 506, 535, 661, 671, 678
MspR9I CCNGG 3 cut(s) 551, 678, 771
MssI GTTTAAAC 1 cut(s) 502
MvaI CCWGG 2 cut(s) 551, 771
MvnI CGCG 3 cut(s) 72, 144, 265
MwoI GCNNNNNNNGC 6 cut(s) 17, 332, 578, 661, 736, 791
NaeI GCCGGC 2 cut(s) 139, 423
NciI CCSGG 1 cut(s) 678
NdeII GATC 2 cut(s) 225, 259
NgoMIV GCCGGC 2 cut(s) 137, 421
NlaIII CATG 4 cut(s) 64, 190, 496, 721
NlaIV GGNNCC 3 cut(s) 282, 533, 675
NmeAIII GCCGAG 1 cut(s) 247
NmuCI GTSAC 1 cut(s) 436
NspV TTCGAA 1 cut(s) 540
PagI TCATGA 2 cut(s) 60, 717
PciSI GCTCTTC 1 cut(s) 464
PcsI WCGNNNNNNNCGW 4 cut(s) 141, 390, 414, 437
PdiI GCCGGC 2 cut(s) 139, 423
PdmI GAANNNNTTC 1 cut(s) 536
PflFI GACNNNGTC 2 cut(s) 11, 34
PinAI ACCGGT 1 cut(s) 534
PkrI GCNGC 5 cut(s) 19, 143, 267, 328, 784
PmaCI CACGTG 1 cut(s) 251
PmeI GTTTAAAC 1 cut(s) 502
PmlI CACGTG 1 cut(s) 251
Ppu21I YACGTR 2 cut(s) 251, 752
Psp6I CCWGG 2 cut(s) 549, 769
PspCI CACGTG 1 cut(s) 251
PspGI CCWGG 2 cut(s) 549, 769
PspN4I GGNNCC 3 cut(s) 282, 533, 675
PspPI GGNCC 2 cut(s) 584, 767
PstI CTGCAG 1 cut(s) 624
PsuI RGATCY 1 cut(s) 225
PsyI GACNNNGTC 2 cut(s) 11, 34
PvuII CAGCTG 1 cut(s) 168
RsaI GTAC 1 cut(s) 380
RsaNI GTAC 1 cut(s) 379
SacII CCGCGG 1 cut(s) 266
SalI GTCGAC 1 cut(s) 77
SapI GCTCTTC 1 cut(s) 464
SaqAI TTAA 2 cut(s) 123, 501
SatI GCNGC 5 cut(s) 18, 142, 266, 327, 783
Sau3AI GATC 2 cut(s) 225, 259
Sau96I GGNCC 2 cut(s) 584, 767
ScrFI CCNGG 3 cut(s) 551, 678, 771
SduI GDGCHC 2 cut(s) 285, 691
SfaNI GCATC 1 cut(s) 123
SfcI CTRYAG 1 cut(s) 620
Sfr303I CCGCGG 1 cut(s) 266
SfuI TTCGAA 1 cut(s) 540
SgrBI CCGCGG 1 cut(s) 266
SgrDI CGTCGACG 1 cut(s) 77
SinI GGWCC 1 cut(s) 584
Sse9I AATT 1 cut(s) 308
SsiI CCGC 9 cut(s) 18, 22, 55, 142, 263, 265, 302, 326, 545
StyD4I CCNGG 3 cut(s) 549, 676, 769
StyI CCWWGG 1 cut(s) 285
TaaI ACNGT 5 cut(s) 36, 235, 436, 513, 557
TaiI ACGT 7 cut(s) 78, 152, 253, 384, 399, 443, 754
TaqI TCGA 7 cut(s) 78, 204, 230, 294, 431, 446, 540
TaqII GACCGA 1 cut(s) 601
TasI AATT 1 cut(s) 308
TauI GCSGC 4 cut(s) 20, 144, 268, 329
Tru1I TTAA 2 cut(s) 123, 501
Tru9I TTAA 2 cut(s) 123, 501
TseFI GTSAC 1 cut(s) 436
TseI GCWGC 1 cut(s) 782
Tsp45I GTSAC 1 cut(s) 436
TspDTI ATGAA 7 cut(s) 17, 49, 77, 482, 641, 706, 734
TspGWI ACGGA 2 cut(s) 290, 349
Tth111I GACNNNGTC 2 cut(s) 11, 34
VpaK11BI GGWCC 1 cut(s) 584
XmiI GTMKAC 1 cut(s) 78
XmnI GAANNNNTTC 1 cut(s) 536
ZraI GACGTC 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.