Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0013864

Physical Location & Seq

Genomic Coordinates Forward (+)

73773702 .. 73776068

Gene Structure 2367 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G042060.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1563 bp

ATGGAAACCCTAACCCTCTTCTTCACCTTAGCAGCTGCTACCTCTTTATATTTCTTCTGGTTCTATCTCCTAGCCCGAAAGCTCACCGGCCCAAAAGTCTGGCCTGTCGTCGGTAGCCTTCCCGTACTCTTCACAAATCGCAACCGAATCCACGACTGGATCGCAAGCAACCTCCGCGCCACCGGGGGATCATCCACGTACCAAACATGCACCATCGCGCTCCCTTTCTTGGCACGCAAGCAAGGGCTGTTTACGGTGACATGTCACCCGAAGAACATCGAGCACATTCTAAGGACCCGATTCGACAACTATCCGAAAGGGCCGTCGTGGCAGGCGGCATTTCATGATCTCTTGGGGGAGGGGATATTTAATAGTGATGGGGAGACATGGCTGATACAGAGGAAGACGGCGGCGCTGGAGTTCACGACAAGGACGCTGAGGCAGGCCATGGCCAGGTGGGTGAATAGAACTATTCGGAACCGGCTATGGTGCATTTTGGACAAGGCTGCCAAGGATCATACTGCGGTGGACTTGCAGGACCTGTTGCTCCGATTGACGTTCGACAACATTTGCGGACTCACATTCGGCAAAGACCCCGAAACACTGTCGCCGGACTTACCCGACAACCCATTTTCCAAGGCCTTTGACAACGCCACAGAAGCCACACTCCAACGTCTCCTTTACCCATCATTCCTATGGAAACTCCAGAACACCTTCGACCTCGGATCCGAGAAACAGCTAAGGAGGAGTCTCGCAATCGTCGGAAACTACATGGACGACGCCGTCGCCGACCGCAAAGCTCGTCCCTCCGACGACCTAATCTCCCGCTTCCTGAACAAGCGCGACGTCGACGGCAACCCTTTCCCGAGCTCAGTCCTCCAGCGCATCGCCCTCAACTTCGTCCTCGCAGGACGCGACACGTCGTCGGTGGCGCTCAGCTGGTTCTTCTGGCTCGTCATGAACAACCCCGAAGTCGAGGACAAGATCGTCAACGAGATCACCACGGTTCTGAGCGAGACACGTGGCATAGACCCGAAGCTTTGGGTGGAGGAGCCGTTGGTGTTCGACGAGGCGGATAAGTTGGTTTACCTAAAAGCGGCGCTGGCGGAGACTCTAAGGTTGTACCCGTCCGTACCGTTGGATTTCAAGTACGTGATCAACGACGACGTTTTACCCGACGGGACGTTTGTTCCGTCCGGTTCAACCGTTACGTACTCGATATATTCTGTGGGGAGGATGAAGACTATTTGGGGAGAGGACTGCATGGAGTTTAAACCAGACCGGTGGCTGTCTTCGGACCAGACCCGGTTCGAACCGCCAAAGGATGGTTACAAGTTCGTGGCGTTTAACGCTGGACCGAGGACTTGTTTGGGAAAGGACCTTGCTTACCTACAAATGAAGTCCGTGGCCTCCGCAGTGCTGCTGCGGTACCGCCTCTCGCCGGTTCCCGGCCACCGAGTGGTGCAGAAGATGTCTCTCACTCTCTTCATGAAGCATGGGCTCAAGGTGTATATGCAGCCCCGCAAGCTCGATGGACCGGGTCGGGTTGCCACGTCAGGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0006082 GO:0006629 GO:0006631 GO:0006725 GO:0008150 GO:0008152 GO:0009058 GO:0009698 GO:0009699 GO:0009987 GO:0010345 GO:0016491 GO:0016705 GO:0016713 GO:0018685 GO:0019438 GO:0019748 GO:0019752 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0055114 GO:0071704 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00073 map00073

Pfam Domains

Protein Families

p450

Protein Analysis

520

Amino Acids

59.06

Weight (kDa)

9.08

Isoelectric Point (pI)

43.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
p450	PF00067	84 - 499	1.3e-62	Cytochrome P450

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013864)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G58860
fragaria_vesca	FvH4_3g38270
malus_domestica	MD03G1073600.v1.1 MD11G1079600.v1.1
prunus_persica	Prupe.6G060200_v2.0.a1
pyrus_communis	pycom03g05950 pycom11g06710
rosa_chinensis	RchiOBHm_Chr5g0068771
rosa_laevigata	RLG00000036009
rosa_multiflora	Rmu_sc0000376.1_g000036
rosa_roxburghii	Rroxscaffold_1G00012050
rosa_rugosa	Rorug05G0393100
rosa_samantha	Rh5AG450600 Rh5BG469100 Rh5CG491300 Rh5DG482400
rosa_wichuraiana	Rw5G042060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	782, 986
AatII	GACGTC	1 cut(s)	849
Acc65I	GGTACC	1 cut(s)	1428
AccB1I	GGYRCC	1 cut(s)	1428
AccB7I	CCANNNNNTGG	2 cut(s)	1325, 1459
AccI	GTMKAC	1 cut(s)	849
AccII	CGCG	4 cut(s)	177, 218, 843, 915
AclWI	GGATC	5 cut(s)	167, 196, 522, 720, 733
AcoI	YGGCCR	2 cut(s)	450, 1450
AcvI	CACGTG	1 cut(s)	1022
AcyI	GRCGYC	2 cut(s)	780, 846
AdeI	CACNNNGTG	1 cut(s)	1459
AfaI	GTAC	7 cut(s)	126, 200, 1124, 1134, 1151, 1214, 1430
AfiI	CCNNNNNNNGG	8 cut(s)	110, 229, 453, 1096, 1325, 1441, 1448, 1459
AflIII	ACRYGT	3 cut(s)	260, 918, 1019
AgeI	ACCGGT	1 cut(s)	1281
AgsI	TTSAA	2 cut(s)	1147, 1203
AhdI	GACNNNNNGTC	1 cut(s)	922
AjiI	CACGTC	2 cut(s)	921, 1554
AjnI	CCWGG	1 cut(s)	452
AluBI	AGCT	8 cut(s)	35, 82, 739, 800, 870, 939, 1039, 1528
AluI	AGCT	8 cut(s)	35, 82, 739, 800, 870, 939, 1039, 1528
Alw21I	GWGCWC	2 cut(s)	285, 872
Alw26I	GTCTC	6 cut(s)	377, 680, 755, 1010, 1103, 1479
AlwI	GGATC	5 cut(s)	167, 196, 522, 720, 733
AlwNI	CAGNNNCTG	1 cut(s)	541
Ama87I	CYCGRG	1 cut(s)	865
AoxI	GGCC	8 cut(s)	88, 101, 320, 444, 450, 639, 1407, 1450
ApeKI	GCWGC	6 cut(s)	32, 35, 506, 1420, 1423, 1516
ArsI	GACNNNNNNTTYG	4 cut(s)	308, 340, 1169, 1201
AsiGI	ACCGGT	1 cut(s)	1281
Asp700I	GAANNNNTTC	1 cut(s)	713
Asp718I	GGTACC	1 cut(s)	1428
AspLEI	GCGC	7 cut(s)	179, 220, 415, 843, 885, 934, 1102
AspS9I	GGNCC	8 cut(s)	89, 294, 320, 538, 1297, 1355, 1378, 1535
AsuC2I	CCSGG	4 cut(s)	184, 1306, 1449, 1539
AsuHPI	GGTGA	6 cut(s)	16, 76, 257, 268, 472, 991
AsuII	TTCGAA	1 cut(s)	1311
AvaI	CYCGRG	1 cut(s)	865
AvaII	GGWCC	6 cut(s)	294, 538, 1297, 1355, 1378, 1535
BalI	TGGCCA	1 cut(s)	452
BamHI	GGATCC	1 cut(s)	725
BanI	GGYRCC	1 cut(s)	1428
BanII	GRGCYC	2 cut(s)	872, 1503
BbrPI	CACGTG	1 cut(s)	1022
BbsI	GAAGAC	3 cut(s)	410, 1247, 1284
Bbv12I	GWGCWC	2 cut(s)	285, 872
BbvCI	CCTCAGC	1 cut(s)	437
BbvI	GCAGC	6 cut(s)	22, 44, 493, 1407, 1410, 1528
BccI	CCATC	5 cut(s)	221, 371, 694, 1319, 1526
BceAI	ACGGC	5 cut(s)	307, 423, 767, 868, 1039
BciT130I	CCWGG	1 cut(s)	454
BclI	TGATCA	1 cut(s)	1155
BcnI	CCSGG	4 cut(s)	184, 1306, 1449, 1539
BcoDI	GTCTC	6 cut(s)	377, 680, 755, 1010, 1103, 1479
BfaI	CTAG	1 cut(s)	71
BfoI	RGCGCY	3 cut(s)	416, 935, 1103
BglI	GCCNNNNNGGC	1 cut(s)	328
BisI	GCNGC	9 cut(s)	33, 36, 336, 411, 507, 1098, 1421, 1424, 1517
BlpI	GCTNAGC	1 cut(s)	935
BlsI	GCNGC	9 cut(s)	34, 37, 337, 412, 508, 1099, 1422, 1425, 1518
Bme1390I	CCNGG	5 cut(s)	184, 454, 1306, 1449, 1539
Bme18I	GGWCC	6 cut(s)	294, 538, 1297, 1355, 1378, 1535
BmeRI	GACNNNNNGTC	1 cut(s)	922
BmeT110I	CYCGRG	1 cut(s)	865
BmgBI	CACGTC	2 cut(s)	921, 1554
BmgT120I	GGNCC	8 cut(s)	89, 294, 320, 538, 1297, 1355, 1378, 1535
BmiI	GGNNCC	6 cut(s)	296, 479, 727, 1053, 1430, 1446
BmrFI	CCNGG	5 cut(s)	184, 454, 1306, 1449, 1539
BmsI	GCATC	1 cut(s)	894
BpiI	GAAGAC	3 cut(s)	410, 1247, 1284
BpmI	CTGGAG	3 cut(s)	437, 689, 863
Bpu10I	CCTNAGC	3 cut(s)	28, 437, 740
Bpu1102I	GCTNAGC	1 cut(s)	935
Bpu14I	TTCGAA	1 cut(s)	1311
BpuEI	CTTGAG	1 cut(s)	1487
BpuMI	CCSGG	4 cut(s)	184, 1306, 1449, 1539
BsaAI	YACGTR	4 cut(s)	198, 1022, 1153, 1212
BsaHI	GRCGYC	2 cut(s)	780, 846
BsaJI	CCNNGG	8 cut(s)	183, 447, 510, 636, 721, 1002, 1358, 1404
BsaWI	WCCGGW	2 cut(s)	1196, 1281
BsaXI	ACNNNNNCTCC	4 cut(s)	806, 836, 1040, 1070
Bsc4I	CCNNNNNNNGG	8 cut(s)	110, 229, 453, 1096, 1325, 1441, 1448, 1459
Bse118I	RCCGGY	4 cut(s)	86, 480, 1281, 1441
Bse1I	ACTGG	1 cut(s)	161
BseBI	CCWGG	1 cut(s)	454
BseDI	CCNNGG	8 cut(s)	183, 447, 510, 636, 721, 1002, 1358, 1404
BseGI	GGATG	3 cut(s)	191, 1242, 1330
BseLI	CCNNNNNNNGG	8 cut(s)	110, 229, 453, 1096, 1325, 1441, 1448, 1459
BseMII	CTCAG	4 cut(s)	428, 885, 949, 1001
BseNI	ACTGG	1 cut(s)	161
BseRI	GAGGAG	2 cut(s)	760, 1064
BseXI	GCAGC	6 cut(s)	22, 44, 493, 1407, 1410, 1528
BsgI	GTGCAG	1 cut(s)	1484
Bsh1236I	CGCG	4 cut(s)	177, 218, 843, 915
Bsh1285I	CGRYCG	1 cut(s)	793
BshFI	GGCC	8 cut(s)	90, 103, 322, 446, 452, 641, 1409, 1452
BshNI	GGYRCC	1 cut(s)	1428
BshTI	ACCGGT	1 cut(s)	1281
BsiEI	CGRYCG	1 cut(s)	793
BsiHKAI	GWGCWC	2 cut(s)	285, 872
BsiHKCI	CYCGRG	1 cut(s)	865
BslFI	GGGAC	2 cut(s)	789, 1195
BslI	CCNNNNNNNGG	8 cut(s)	110, 229, 453, 1096, 1325, 1441, 1448, 1459
BsmAI	GTCTC	6 cut(s)	377, 680, 755, 1010, 1103, 1479
BsmBI	CGTCTC	1 cut(s)	680
BsmFI	GGGAC	2 cut(s)	789, 1195
BsnI	GGCC	8 cut(s)	90, 103, 322, 446, 452, 641, 1409, 1452
BsoBI	CYCGRG	1 cut(s)	865
Bsp119I	TTCGAA	1 cut(s)	1311
Bsp1286I	GDGCHC	3 cut(s)	285, 872, 1503
Bsp143I	GATC	8 cut(s)	159, 188, 346, 514, 725, 984, 996, 1155
Bsp1720I	GCTNAGC	1 cut(s)	935
Bsp19I	CCATGG	1 cut(s)	447
BspANI	GGCC	8 cut(s)	90, 103, 322, 446, 452, 641, 1409, 1452
BspCNI	CTCAG	4 cut(s)	429, 884, 948, 1002
BspFNI	CGCG	4 cut(s)	177, 218, 843, 915
BspHI	TCATGA	3 cut(s)	343, 957, 1488
BspLI	GGNNCC	6 cut(s)	296, 479, 727, 1053, 1430, 1446
BspPI	GGATC	5 cut(s)	167, 196, 522, 720, 733
BspT104I	TTCGAA	1 cut(s)	1311
BspT107I	GGYRCC	1 cut(s)	1428
BsrFI	RCCGGY	4 cut(s)	86, 480, 1281, 1441
BsrI	ACTGG	1 cut(s)	161
BssAI	RCCGGY	4 cut(s)	86, 480, 1281, 1441
BssECI	CCNNGG	8 cut(s)	183, 447, 510, 636, 721, 1002, 1358, 1404
BssMI	GATC	8 cut(s)	159, 188, 346, 514, 725, 984, 996, 1155
BssNI	GRCGYC	2 cut(s)	780, 846
BssT1I	CCWWGG	3 cut(s)	447, 510, 636
Bst2UI	CCWGG	1 cut(s)	454
Bst4CI	ACNGT	5 cut(s)	256, 606, 1006, 1137, 1207
Bst6I	CTCTTC	3 cut(s)	23, 134, 1490
BstACI	GRCGYC	2 cut(s)	780, 846
BstBAI	YACGTR	4 cut(s)	198, 1022, 1153, 1212
BstBI	TTCGAA	1 cut(s)	1311
BstC8I	GCNNGC	7 cut(s)	166, 235, 239, 333, 444, 1104, 1526
BstDEI	CTNAG	8 cut(s)	28, 290, 437, 740, 871, 935, 1010, 1115
BstDSI	CCRYGG	3 cut(s)	447, 1002, 1404
BstF5I	GGATG	3 cut(s)	191, 1242, 1330
BstFNI	CGCG	4 cut(s)	177, 218, 843, 915
BstH2I	RGCGCY	3 cut(s)	416, 935, 1103
BstHHI	GCGC	7 cut(s)	179, 220, 415, 843, 885, 934, 1102
BstKTI	GATC	8 cut(s)	162, 191, 349, 517, 728, 987, 999, 1158
BstMAI	GTCTC	6 cut(s)	377, 680, 755, 1010, 1103, 1479
BstMBI	GATC	8 cut(s)	159, 188, 346, 514, 725, 984, 996, 1155
BstMCI	CGRYCG	1 cut(s)	793
BstMWI	GCNNNNNNNGC	6 cut(s)	174, 328, 659, 1103, 1349, 1525
BstNI	CCWGG	1 cut(s)	454
BstNSI	RCATGY	2 cut(s)	210, 264
BstSCI	CCNGG	5 cut(s)	182, 452, 1304, 1447, 1537
BstSNI	TACGTA	1 cut(s)	1212
BstUI	CGCG	4 cut(s)	177, 218, 843, 915
BstV1I	GCAGC	6 cut(s)	22, 44, 493, 1407, 1410, 1528
BstV2I	GAAGAC	3 cut(s)	410, 1247, 1284
BstX2I	RGATCY	1 cut(s)	725
BstXI	CCANNNNNNTGG	2 cut(s)	99, 1284
BstYI	RGATCY	1 cut(s)	725
BsuRI	GGCC	8 cut(s)	90, 103, 322, 446, 452, 641, 1409, 1452
BtgI	CCRYGG	3 cut(s)	447, 1002, 1404
BtgZI	GCGATG	2 cut(s)	199, 871
BtrI	CACGTC	2 cut(s)	921, 1554
BtsCI	GGATG	3 cut(s)	191, 1242, 1330
BtsI	GCAGTG	1 cut(s)	1422
BtsIMutI	CAGTG	2 cut(s)	602, 1422
Cac8I	GCNNGC	7 cut(s)	166, 235, 239, 333, 444, 1104, 1526
CaiI	CAGNNNCTG	1 cut(s)	541
CciI	TCATGA	3 cut(s)	343, 957, 1488
CfoI	GCGC	7 cut(s)	179, 220, 415, 843, 885, 934, 1102
Cfr10I	RCCGGY	4 cut(s)	86, 480, 1281, 1441
Cfr13I	GGNCC	8 cut(s)	89, 294, 320, 538, 1297, 1355, 1378, 1535
CseI	GACGC	3 cut(s)	442, 788, 921
Csp6I	GTAC	7 cut(s)	125, 199, 1123, 1133, 1150, 1213, 1429
CspAI	ACCGGT	1 cut(s)	1281
CviQI	GTAC	7 cut(s)	125, 199, 1123, 1133, 1150, 1213, 1429
DdeI	CTNAG	8 cut(s)	28, 290, 437, 740, 871, 935, 1010, 1115
DpnI	GATC	8 cut(s)	161, 190, 348, 516, 727, 986, 998, 1157
DpnII	GATC	8 cut(s)	159, 188, 346, 514, 725, 984, 996, 1155
DraI	TTTAAA	1 cut(s)	1273
DraIII	CACNNNGTG	1 cut(s)	1459
DrdI	GACNNNNNNGTC	2 cut(s)	782, 986
DriI	GACNNNNNGTC	1 cut(s)	922
DseDI	GACNNNNNNGTC	2 cut(s)	782, 986
EaeI	YGGCCR	2 cut(s)	450, 1450
Eam1104I	CTCTTC	3 cut(s)	23, 134, 1490
Eam1105I	GACNNNNNGTC	1 cut(s)	922
EarI	CTCTTC	3 cut(s)	23, 134, 1490
EciI	GGCGGA	2 cut(s)	1088, 1121
Ecl136II	GAGCTC	1 cut(s)	870
Eco105I	TACGTA	1 cut(s)	1212
Eco130I	CCWWGG	3 cut(s)	447, 510, 636
Eco147I	AGGCCT	1 cut(s)	641
Eco24I	GRGCYC	2 cut(s)	872, 1503
Eco47I	GGWCC	6 cut(s)	294, 538, 1297, 1355, 1378, 1535
Eco53kI	GAGCTC	1 cut(s)	870
Eco72I	CACGTG	1 cut(s)	1022
Eco88I	CYCGRG	1 cut(s)	865
EcoICRI	GAGCTC	1 cut(s)	870
EcoO109I	RGGNCCY	3 cut(s)	294, 538, 1378
EcoRII	CCWGG	1 cut(s)	452
EcoT14I	CCWWGG	3 cut(s)	447, 510, 636
EcoT38I	GRGCYC	2 cut(s)	872, 1503
ErhI	CCWWGG	3 cut(s)	447, 510, 636
Esp3I	CGTCTC	1 cut(s)	680
FaqI	GGGAC	2 cut(s)	789, 1195
FauI	CCCGC	2 cut(s)	833, 1529
FbaI	TGATCA	1 cut(s)	1155
FblI	GTMKAC	1 cut(s)	849
Fnu4HI	GCNGC	9 cut(s)	33, 36, 336, 411, 507, 1098, 1421, 1424, 1517
FokI	GGATG	3 cut(s)	178, 1249, 1337
FriOI	GRGCYC	2 cut(s)	872, 1503
Fsp4HI	GCNGC	9 cut(s)	33, 36, 336, 411, 507, 1098, 1421, 1424, 1517
FspBI	CTAG	1 cut(s)	71
GlaI	GCGC	7 cut(s)	178, 219, 414, 842, 884, 933, 1101
GluI	GCNGC	9 cut(s)	33, 36, 336, 411, 507, 1098, 1421, 1424, 1517
GsuI	CTGGAG	3 cut(s)	437, 689, 863
HaeII	RGCGCY	3 cut(s)	416, 935, 1103
HaeIII	GGCC	8 cut(s)	90, 103, 322, 446, 452, 641, 1409, 1452
HgaI	GACGC	3 cut(s)	442, 788, 921
HhaI	GCGC	7 cut(s)	179, 220, 415, 843, 885, 934, 1102
Hin1I	GRCGYC	2 cut(s)	780, 846
Hin6I	GCGC	7 cut(s)	177, 218, 413, 841, 883, 932, 1100
HinP1I	GCGC	7 cut(s)	177, 218, 413, 841, 883, 932, 1100
HincII	GTYRAC	2 cut(s)	850, 991
HindII	GTYRAC	2 cut(s)	850, 991
HindIII	AAGCTT	1 cut(s)	1037
HinfI	GANTC	5 cut(s)	147, 300, 576, 748, 1111
HphI	GGTGA	6 cut(s)	16, 76, 257, 268, 472, 991
Hpy166II	GTNNAC	6 cut(s)	252, 423, 529, 850, 991, 1087
Hpy188I	TCNGA	9 cut(s)	315, 477, 551, 725, 730, 764, 811, 1011, 1297
Hpy188III	TCNNGA	8 cut(s)	344, 424, 706, 832, 865, 958, 1489, 1557
Hpy8I	GTNNAC	6 cut(s)	252, 423, 529, 850, 991, 1087
HpyAV	CCTTC	2 cut(s)	128, 724
HpyCH4III	ACNGT	5 cut(s)	256, 606, 1006, 1137, 1207
HpyCH4V	TGCA	6 cut(s)	210, 492, 535, 1263, 1465, 1516
HpyF10VI	GCNNNNNNNGC	6 cut(s)	174, 328, 659, 1103, 1349, 1525
HpyF3I	CTNAG	8 cut(s)	28, 290, 437, 740, 871, 935, 1010, 1115
Hsp92I	GRCGYC	2 cut(s)	780, 846
HspAI	GCGC	7 cut(s)	177, 218, 413, 841, 883, 932, 1100
KpnI	GGTACC	1 cut(s)	1432
Ksp22I	TGATCA	1 cut(s)	1155
Kzo9I	GATC	8 cut(s)	159, 188, 346, 514, 725, 984, 996, 1155
LmnI	GCTCC	3 cut(s)	225, 552, 1051
Lsp1109I	GCAGC	6 cut(s)	22, 44, 493, 1407, 1410, 1528
LweI	GCATC	1 cut(s)	894
MaeI	CTAG	1 cut(s)	71
MaeIII	GTNAC	4 cut(s)	256, 263, 1207, 1328
MalI	GATC	8 cut(s)	161, 190, 348, 516, 727, 986, 998, 1157
MboI	GATC	8 cut(s)	159, 188, 346, 514, 725, 984, 996, 1155
MflI	RGATCY	1 cut(s)	725
MhlI	GDGCHC	3 cut(s)	285, 872, 1503
MlsI	TGGCCA	1 cut(s)	452
MluNI	TGGCCA	1 cut(s)	452
MlyI	GAGTC	3 cut(s)	570, 757, 1105
MmeI	TCCRAC	4 cut(s)	694, 742, 834, 1119
Mox20I	TGGCCA	1 cut(s)	452
MroXI	GAANNNNTTC	1 cut(s)	713
MscI	TGGCCA	1 cut(s)	452
MseI	TTAA	3 cut(s)	369, 1272, 1347
MslI	CAYNNNNRTG	1 cut(s)	694
Msp20I	TGGCCA	1 cut(s)	452
MspA1I	CMGCKG	2 cut(s)	35, 939
MspR9I	CCNGG	5 cut(s)	184, 454, 1306, 1449, 1539
MssI	GTTTAAAC	1 cut(s)	1273
MvaI	CCWGG	1 cut(s)	454
MvnI	CGCG	4 cut(s)	177, 218, 843, 915
MwoI	GCNNNNNNNGC	6 cut(s)	174, 328, 659, 1103, 1349, 1525
NciI	CCSGG	4 cut(s)	184, 1306, 1449, 1539
NcoI	CCATGG	1 cut(s)	447
NdeII	GATC	8 cut(s)	159, 188, 346, 514, 725, 984, 996, 1155
NlaIV	GGNNCC	6 cut(s)	296, 479, 727, 1053, 1430, 1446
NmuCI	GTSAC	2 cut(s)	256, 263
NspI	RCATGY	2 cut(s)	210, 264
NspV	TTCGAA	1 cut(s)	1311
PagI	TCATGA	3 cut(s)	343, 957, 1488
PceI	AGGCCT	1 cut(s)	641
PciI	ACATGT	1 cut(s)	260
PcsI	WCGNNNNNNNCGW	5 cut(s)	786, 912, 1158, 1173, 1190
PdmI	GAANNNNTTC	1 cut(s)	713
PfeI	GAWTC	2 cut(s)	147, 300
PflFI	GACNNNGTC	2 cut(s)	782, 1539
PflMI	CCANNNNNTGG	2 cut(s)	1325, 1459
PinAI	ACCGGT	1 cut(s)	1281
PkrI	GCNGC	9 cut(s)	34, 37, 337, 412, 508, 1099, 1422, 1425, 1518
PleI	GAGTC	3 cut(s)	570, 756, 1105
PmaCI	CACGTG	1 cut(s)	1022
PmeI	GTTTAAAC	1 cut(s)	1273
PmlI	CACGTG	1 cut(s)	1022
PpsI	GAGTC	3 cut(s)	570, 756, 1105
Ppu21I	YACGTR	4 cut(s)	198, 1022, 1153, 1212
PpuMI	RGGWCCY	3 cut(s)	294, 538, 1378
PscI	ACATGT	1 cut(s)	260
Psp124BI	GAGCTC	1 cut(s)	872
Psp5II	RGGWCCY	3 cut(s)	294, 538, 1378
Psp6I	CCWGG	1 cut(s)	452
PspCI	CACGTG	1 cut(s)	1022
PspGI	CCWGG	1 cut(s)	452
PspN4I	GGNNCC	6 cut(s)	296, 479, 727, 1053, 1430, 1446
PspPI	GGNCC	8 cut(s)	89, 294, 320, 538, 1297, 1355, 1378, 1535
PspPPI	RGGWCCY	3 cut(s)	294, 538, 1378
PstNI	CAGNNNCTG	1 cut(s)	541
PsuI	RGATCY	1 cut(s)	725
PsyI	GACNNNGTC	2 cut(s)	782, 1539
PvuII	CAGCTG	2 cut(s)	35, 939
RsaI	GTAC	7 cut(s)	126, 200, 1124, 1134, 1151, 1214, 1430
RsaNI	GTAC	7 cut(s)	125, 199, 1123, 1133, 1150, 1213, 1429
RseI	CAYNNNNRTG	1 cut(s)	694
SacI	GAGCTC	1 cut(s)	872
SalI	GTCGAC	1 cut(s)	848
SaqAI	TTAA	3 cut(s)	369, 1272, 1347
SatI	GCNGC	9 cut(s)	33, 36, 336, 411, 507, 1098, 1421, 1424, 1517
Sau3AI	GATC	8 cut(s)	159, 188, 346, 514, 725, 984, 996, 1155
Sau96I	GGNCC	8 cut(s)	89, 294, 320, 538, 1297, 1355, 1378, 1535
SchI	GAGTC	3 cut(s)	570, 757, 1105
ScrFI	CCNGG	5 cut(s)	184, 454, 1306, 1449, 1539
SduI	GDGCHC	3 cut(s)	285, 872, 1503
SfaNI	GCATC	1 cut(s)	894
SfuI	TTCGAA	1 cut(s)	1311
SgrDI	CGTCGACG	1 cut(s)	848
SinI	GGWCC	6 cut(s)	294, 538, 1297, 1355, 1378, 1535
SmiMI	CAYNNNNRTG	1 cut(s)	694
SmlI	CTYRAG	1 cut(s)	1502
SmoI	CTYRAG	1 cut(s)	1502
SnaBI	TACGTA	1 cut(s)	1212
SseBI	AGGCCT	1 cut(s)	641
SspMI	CTAG	1 cut(s)	71
SstI	GAGCTC	1 cut(s)	872
StuI	AGGCCT	1 cut(s)	641
StyD4I	CCNGG	5 cut(s)	182, 452, 1304, 1447, 1537
StyI	CCWWGG	3 cut(s)	447, 510, 636
TaaI	ACNGT	5 cut(s)	256, 606, 1006, 1137, 1207
TaqII	GACCGA	1 cut(s)	1372
TauI	GCSGC	3 cut(s)	338, 413, 1100
TfiI	GAWTC	2 cut(s)	147, 300
Tru1I	TTAA	3 cut(s)	369, 1272, 1347
Tru9I	TTAA	3 cut(s)	369, 1272, 1347
TscAI	CASTG	2 cut(s)	609, 1422
TseFI	GTSAC	2 cut(s)	256, 263
TseI	GCWGC	6 cut(s)	32, 35, 506, 1420, 1423, 1516
Tsp45I	GTSAC	2 cut(s)	256, 263
TspDTI	ATGAA	6 cut(s)	332, 974, 1253, 1412, 1477, 1505
TspGWI	ACGGA	3 cut(s)	1120, 1182, 1393
TspRI	CASTG	2 cut(s)	609, 1422
Tth111I	GACNNNGTC	2 cut(s)	782, 1539
Van91I	CCANNNNNTGG	2 cut(s)	1325, 1459
VpaK11BI	GGWCC	6 cut(s)	294, 538, 1297, 1355, 1378, 1535
XceI	RCATGY	2 cut(s)	210, 264
XcmI	CCANNNNNNNNNTGG	1 cut(s)	693
XmiI	GTMKAC	1 cut(s)	849
XmnI	GAANNNNTTC	1 cut(s)	713
XspI	CTAG	1 cut(s)	71
ZraI	GACGTC	1 cut(s)	847

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.