pycom11g18110

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0028902
Physical Location & Seq
Reverse (-)
20356651 .. 20357301
651 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 651 bp
ATGCCTTGTTCGCGGCGTGACTACTGCAGCTGCCTTGGTGTCATCCTCACCATGCTCACCTTCCTCGCCTTTCTCGGGGTAATAGCCTTTTCCTTCTACTCCTTAATCTGGTTGTCTGTCCTTCCCAATGAGCCCAAAATCACCATCACTGACGCATCTCTCACCCAATTTGATTTCGCTGCCGGCAGAAACAACATTCTCAACTACAACCTTGCACTCAACTTTACCATCAGAAATTCCAACAAAAGGGCCGGCATAGACTACCGTGGCATCCAAGTCACTGGCAAATACAGAAACAAGAAGTTTTTTGTTAAGAGTTTGAGTTCGACACCTTTTTACCAAGACCACAAGAACACCACCGTTGTGCCTGTAGTTCTTCAAGGGCAGCAGTGGGTTAAGTTTCGAAAACATGACGTTTCTAAGTTTAACTCGGAGACTGCGGCCGGTGTTTACAACATTCGTGTAGAGTTTCGTCTTCGGATCAGATACAAACGCGTCAGGATTTTGAAGAAAATTACGGACTCGCCGAACGTTGGATCGATCTATTGCAAGCAAGTGAAGGTTCCATTGAGTGGGTATAATGAAACATCTGCAGGGGGTTTCAAATTCAACCCTACCAAGTGCAAGAATGTTCCAATGAAAGGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

217

Amino Acids

24.66

Weight (kDa)

10.16

Isoelectric Point (pI)

41.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 75 - 169 5.8e-09 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028902)

Species Orthologous Gene IDs
malus_domestica MD11G1207900.v1.1
pyrus_communis pycom11g18110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 572
AccII CGCG 2 cut(s) 13, 495
AciI CCGC 2 cut(s) 13, 440
AclI AACGTT 1 cut(s) 531
AclWI GGATC 2 cut(s) 488, 544
AcoI YGGCCR 1 cut(s) 441
AcsI RAATTY 2 cut(s) 235, 605
AfiI CCNNNNNNNGG 6 cut(s) 75, 108, 246, 533, 572, 641
AflIII ACRYGT 1 cut(s) 493
AgsI TTSAA 4 cut(s) 380, 508, 604, 610
AleI CACNNNNGTG 1 cut(s) 362
AluBI AGCT 1 cut(s) 30
AluI AGCT 1 cut(s) 30
Alw26I GTCTC 1 cut(s) 428
AlwI GGATC 2 cut(s) 488, 544
Ama87I CYCGRG 1 cut(s) 74
AoxI GGCC 2 cut(s) 249, 441
ApeKI GCWGC 4 cut(s) 27, 30, 179, 385
ApoI RAATTY 2 cut(s) 235, 605
AspS9I GGNCC 1 cut(s) 249
AsuHPI GGTGA 4 cut(s) 40, 49, 133, 154
AsuII TTCGAA 1 cut(s) 403
AvaI CYCGRG 1 cut(s) 74
BanII GRGCYC 1 cut(s) 135
BbsI GAAGAC 1 cut(s) 467
BbvI GCAGC 4 cut(s) 17, 39, 166, 397
BccI CCATC 2 cut(s) 152, 236
BcoDI GTCTC 1 cut(s) 428
BfmI CTRYAG 3 cut(s) 25, 369, 591
BisI GCNGC 6 cut(s) 14, 28, 31, 180, 386, 441
BlsI GCNGC 6 cut(s) 15, 29, 32, 181, 387, 442
BmeT110I CYCGRG 1 cut(s) 74
BmgT120I GGNCC 1 cut(s) 249
BmiI GGNNCC 1 cut(s) 564
BmsI GCATC 2 cut(s) 164, 279
BpiI GAAGAC 1 cut(s) 467
Bpu14I TTCGAA 1 cut(s) 403
Bsa29I ATCGAT 1 cut(s) 539
BsaJI CCNNGG 2 cut(s) 34, 265
Bsc4I CCNNNNNNNGG 6 cut(s) 75, 108, 246, 533, 572, 641
Bse118I RCCGGY 3 cut(s) 182, 251, 443
Bse1I ACTGG 1 cut(s) 286
BseCI ATCGAT 1 cut(s) 539
BseDI CCNNGG 2 cut(s) 34, 265
BseGI GGATG 2 cut(s) 42, 270
BseLI CCNNNNNNNGG 6 cut(s) 75, 108, 246, 533, 572, 641
BseNI ACTGG 1 cut(s) 286
BseX3I CGGCCG 1 cut(s) 441
BseXI GCAGC 4 cut(s) 17, 39, 166, 397
Bsh1236I CGCG 2 cut(s) 13, 495
Bsh1285I CGRYCG 1 cut(s) 444
BshFI GGCC 2 cut(s) 251, 443
BshVI ATCGAT 1 cut(s) 539
BsiEI CGRYCG 1 cut(s) 444
BsiHKCI CYCGRG 1 cut(s) 74
BsiSI CCGG 3 cut(s) 183, 252, 444
BslI CCNNNNNNNGG 6 cut(s) 75, 108, 246, 533, 572, 641
BsmAI GTCTC 1 cut(s) 428
BsnI GGCC 2 cut(s) 251, 443
BsoBI CYCGRG 1 cut(s) 74
Bsp119I TTCGAA 1 cut(s) 403
Bsp1286I GDGCHC 1 cut(s) 135
Bsp143I GATC 3 cut(s) 480, 536, 540
BspACI CCGC 2 cut(s) 13, 440
BspANI GGCC 2 cut(s) 251, 443
BspDI ATCGAT 1 cut(s) 539
BspFNI CGCG 2 cut(s) 13, 495
BspLI GGNNCC 1 cut(s) 564
BspMAI CTGCAG 2 cut(s) 29, 595
BspPI GGATC 2 cut(s) 488, 544
BspT104I TTCGAA 1 cut(s) 403
BsrFI RCCGGY 3 cut(s) 182, 251, 443
BsrI ACTGG 1 cut(s) 286
BssAI RCCGGY 3 cut(s) 182, 251, 443
BssECI CCNNGG 2 cut(s) 34, 265
BssMI GATC 3 cut(s) 480, 536, 540
BssT1I CCWWGG 1 cut(s) 34
Bst4CI ACNGT 2 cut(s) 266, 361
BstBI TTCGAA 1 cut(s) 403
BstC8I GCNNGC 3 cut(s) 184, 253, 551
BstDEI CTNAG 1 cut(s) 420
BstDSI CCRYGG 1 cut(s) 265
BstF5I GGATG 2 cut(s) 42, 270
BstFNI CGCG 2 cut(s) 13, 495
BstKTI GATC 3 cut(s) 483, 539, 543
BstMAI GTCTC 1 cut(s) 428
BstMBI GATC 3 cut(s) 480, 536, 540
BstMCI CGRYCG 1 cut(s) 444
BstMWI GCNNNNNNNGC 1 cut(s) 10
BstSFI CTRYAG 3 cut(s) 25, 369, 591
BstUI CGCG 2 cut(s) 13, 495
BstV1I GCAGC 4 cut(s) 17, 39, 166, 397
BstV2I GAAGAC 1 cut(s) 467
BstXI CCANNNNNNTGG 1 cut(s) 281
BstZI CGGCCG 1 cut(s) 441
Bsu15I ATCGAT 1 cut(s) 539
BsuRI GGCC 2 cut(s) 251, 443
BsuTUI ATCGAT 1 cut(s) 539
BtgI CCRYGG 1 cut(s) 265
BtsCI GGATG 2 cut(s) 42, 270
BtsI GCAGTG 1 cut(s) 395
BtsIMutI CAGTG 3 cut(s) 147, 279, 395
Cac8I GCNNGC 3 cut(s) 184, 253, 551
Cfr10I RCCGGY 3 cut(s) 182, 251, 443
Cfr13I GGNCC 1 cut(s) 249
ClaI ATCGAT 1 cut(s) 539
CseI GACGC 2 cut(s) 161, 484
CviAII CATG 2 cut(s) 52, 410
CviJI RGCY 5 cut(s) 30, 86, 133, 251, 443
CviKI_1 RGCY 5 cut(s) 30, 86, 133, 251, 443
DdeI CTNAG 1 cut(s) 420
DpnI GATC 3 cut(s) 482, 538, 542
DpnII GATC 3 cut(s) 480, 536, 540
EaeI YGGCCR 1 cut(s) 441
EagI CGGCCG 1 cut(s) 441
EclXI CGGCCG 1 cut(s) 441
Eco130I CCWWGG 1 cut(s) 34
Eco24I GRGCYC 1 cut(s) 135
Eco52I CGGCCG 1 cut(s) 441
Eco88I CYCGRG 1 cut(s) 74
EcoT14I CCWWGG 1 cut(s) 34
EcoT38I GRGCYC 1 cut(s) 135
ErhI CCWWGG 1 cut(s) 34
FaeI CATG 2 cut(s) 55, 413
FaiI YATR 4 cut(s) 53, 257, 411, 579
FatI CATG 2 cut(s) 51, 409
Fnu4HI GCNGC 6 cut(s) 14, 28, 31, 180, 386, 441
FokI GGATG 2 cut(s) 29, 257
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 6 cut(s) 14, 28, 31, 180, 386, 441
GluI GCNGC 6 cut(s) 14, 28, 31, 180, 386, 441
HaeIII GGCC 2 cut(s) 251, 443
HapII CCGG 3 cut(s) 183, 252, 444
HgaI GACGC 2 cut(s) 161, 484
Hin1II CATG 2 cut(s) 55, 413
HinfI GANTC 1 cut(s) 521
HpaII CCGG 3 cut(s) 183, 252, 444
HphI GGTGA 4 cut(s) 40, 49, 133, 154
Hpy166II GTNNAC 1 cut(s) 451
Hpy188I TCNGA 4 cut(s) 233, 433, 480, 485
Hpy188III TCNNGA 1 cut(s) 499
Hpy8I GTNNAC 1 cut(s) 451
HpyAV CCTTC 4 cut(s) 70, 103, 131, 553
HpyCH4III ACNGT 2 cut(s) 266, 361
HpyCH4IV ACGT 2 cut(s) 414, 531
HpyCH4V TGCA 5 cut(s) 27, 215, 549, 593, 624
HpyF10VI GCNNNNNNNGC 1 cut(s) 10
HpyF3I CTNAG 1 cut(s) 420
HpySE526I ACGT 2 cut(s) 414, 531
Hsp92II CATG 2 cut(s) 55, 413
KroI GCCGGC 2 cut(s) 182, 251
KroNI GCCGGC 2 cut(s) 184, 253
Kzo9I GATC 3 cut(s) 480, 536, 540
LpnPI CCDG 8 cut(s) 94, 196, 265, 267, 381, 457, 484, 579
Lsp1109I GCAGC 4 cut(s) 17, 39, 166, 397
LweI GCATC 2 cut(s) 164, 279
MaeII ACGT 2 cut(s) 414, 531
MaeIII GTNAC 2 cut(s) 17, 277
MalI GATC 3 cut(s) 482, 538, 542
MboI GATC 3 cut(s) 480, 536, 540
MboII GAAGA 3 cut(s) 368, 467, 520
MhlI GDGCHC 1 cut(s) 135
MluCI AATT 4 cut(s) 167, 235, 513, 605
MluI ACGCGT 1 cut(s) 493
MlyI GAGTC 1 cut(s) 515
MmeI TCCRAC 2 cut(s) 264, 514
MnlI CCTC 2 cut(s) 56, 74
MroNI GCCGGC 2 cut(s) 182, 251
MseI TTAA 4 cut(s) 104, 312, 396, 426
MslI CAYNNNNRTG 1 cut(s) 362
MspA1I CMGCKG 1 cut(s) 30
MspI CCGG 3 cut(s) 183, 252, 444
MvnI CGCG 2 cut(s) 13, 495
MwoI GCNNNNNNNGC 1 cut(s) 10
NaeI GCCGGC 2 cut(s) 184, 253
NdeII GATC 3 cut(s) 480, 536, 540
NgoMIV GCCGGC 2 cut(s) 182, 251
NlaIII CATG 2 cut(s) 55, 413
NlaIV GGNNCC 1 cut(s) 564
NmuCI GTSAC 2 cut(s) 17, 277
NspV TTCGAA 1 cut(s) 403
OliI CACNNNNGTG 1 cut(s) 362
PcsI WCGNNNNNNNCGW 1 cut(s) 524
PdiI GCCGGC 2 cut(s) 184, 253
PflMI CCANNNNNTGG 1 cut(s) 572
PkrI GCNGC 6 cut(s) 15, 29, 32, 181, 387, 442
PleI GAGTC 1 cut(s) 515
PpsI GAGTC 1 cut(s) 515
Psp1406I AACGTT 1 cut(s) 531
PspN4I GGNNCC 1 cut(s) 564
PspPI GGNCC 1 cut(s) 249
PstI CTGCAG 2 cut(s) 29, 595
PvuII CAGCTG 1 cut(s) 30
RseI CAYNNNNRTG 1 cut(s) 362
SaqAI TTAA 4 cut(s) 104, 312, 396, 426
SatI GCNGC 6 cut(s) 14, 28, 31, 180, 386, 441
Sau3AI GATC 3 cut(s) 480, 536, 540
Sau96I GGNCC 1 cut(s) 249
SchI GAGTC 1 cut(s) 515
SduI GDGCHC 1 cut(s) 135
SetI ASST 7 cut(s) 32, 62, 213, 334, 417, 534, 564
SfaNI GCATC 2 cut(s) 164, 279
SfcI CTRYAG 3 cut(s) 25, 369, 591
SfuI TTCGAA 1 cut(s) 403
SmiMI CAYNNNNRTG 1 cut(s) 362
Sse9I AATT 4 cut(s) 167, 235, 513, 605
SsiI CCGC 2 cut(s) 13, 440
StyI CCWWGG 1 cut(s) 34
TaaI ACNGT 2 cut(s) 266, 361
TaiI ACGT 2 cut(s) 417, 534
TaqI TCGA 3 cut(s) 326, 403, 539
TasI AATT 4 cut(s) 167, 235, 513, 605
TauI GCSGC 2 cut(s) 16, 443
Tru1I TTAA 4 cut(s) 104, 312, 396, 426
Tru9I TTAA 4 cut(s) 104, 312, 396, 426
TscAI CASTG 3 cut(s) 154, 286, 395
TseFI GTSAC 2 cut(s) 17, 277
TseI GCWGC 4 cut(s) 27, 30, 179, 385
Tsp45I GTSAC 2 cut(s) 17, 277
TspDTI ATGAA 1 cut(s) 597
TspGWI ACGGA 1 cut(s) 533
TspRI CASTG 3 cut(s) 154, 286, 395
Van91I CCANNNNNTGG 1 cut(s) 572
XapI RAATTY 2 cut(s) 235, 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.