pycom11g18850

Belongs to the HisA HisF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0028925
Physical Location & Seq
Forward (+)
21054704 .. 21057307
2604 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 717 bp
ATGGAGGCGCCGCCATTCGCCTCTTCCACCAAGACGCTACCGTTTCGCTCATCCTCCCCTGTATTCCTTCCCAAAACTCTCCTCAATTTCAGACGTCCCAGAAGCTTCTCCGGCCGTGCCTCCGCCGCCGCAGATTCCGTGGTGACTCTGCTCGACTACGGCGCCGGCAACGTTCGCAGCGTGAGGAATGCCATTCGCCACCTTGGCTTCGACGTCAAAGATGTCCAAACTCTGGAAGACATTCTGAATGCCGAACGCCTAGTTTTTCCCGGTGTGGGTGCTTTTGCTGCTGCCATGGATGTGCTGAATAAGAATGGGATGGCGGAAGCAATCTGTTCATATATTGAGAAGGATCGACCATTTCTAGGCATTTGTCTTGGACTTCAACTCCTTTTTGAGTCCAGTGAAGAGAATGGACCAGTGAAGGGTCTTGGCTTGATACGCGGAGTGGTTGGGCGTTTTGATTCATCAAATGGTTTTAGAGTTCCACACATTGGATGGAATGCTTTGAAGATTGAAAAAGACTCATTAATTTTGGATGATGTTGGAAGCCATCATGTCTATTTCGTTCATTCTTACAGAGCCATGCAAATGAAAACAAAGACTGGGTTTCGTCTACTTGCAACTGTGGAGACAATTTTATTGCGTCTATTAGAAGGGGAAATGTGCATGCAGTTCAATTCCACCCGGAAAAGAGTGGAGATGCTGGTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00340 ko01100 ko01110 ko01230 map00340 map01100 map01110 map01230
Pfam Domains
Protein Families
GATase His_biosynth

Protein Analysis

239

Amino Acids

26.36

Weight (kDa)

9.22

Isoelectric Point (pI)

48.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GATase PF00117 50 - 210 1.4e-14 Glutamine amidotransferase class-I
GATase_3 PF07685 74 - 148 1.3e-07 CobB/CobQ-like glutamine amidotransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028925)

Species Orthologous Gene IDs
malus_domestica MD11G1213900.v1.1
pyrus_communis pycom11g18850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 97, 216
AccB1I GGYRCC 2 cut(s) 7, 161
AccB7I CCANNNNNTGG 2 cut(s) 232, 494
AccI GTMKAC 1 cut(s) 616
AccII CGCG 1 cut(s) 444
AciI CCGC 6 cut(s) 11, 123, 126, 129, 323, 444
AclI AACGTT 1 cut(s) 171
AclWI GGATC 1 cut(s) 360
AcoI YGGCCR 1 cut(s) 112
AcyI GRCGYC 4 cut(s) 8, 94, 162, 213
AfiI CCNNNNNNNGG 5 cut(s) 232, 275, 365, 425, 494
AgsI TTSAA 4 cut(s) 386, 511, 518, 679
AluBI AGCT 1 cut(s) 105
AluI AGCT 1 cut(s) 105
Alw26I GTCTC 1 cut(s) 626
AlwI GGATC 1 cut(s) 360
AoxI GGCC 1 cut(s) 112
ApeKI GCWGC 3 cut(s) 177, 287, 290
AseI ATTAAT 1 cut(s) 530
Asp700I GAANNNNTTC 1 cut(s) 240
AspLEI GCGC 2 cut(s) 10, 164
AspS9I GGNCC 1 cut(s) 416
AsuC2I CCSGG 2 cut(s) 270, 688
AsuHPI GGTGA 1 cut(s) 154
AvaII GGWCC 1 cut(s) 416
BanI GGYRCC 2 cut(s) 7, 161
BbsI GAAGAC 1 cut(s) 243
BbvI GCAGC 3 cut(s) 189, 274, 277
BccI CCATC 3 cut(s) 313, 492, 561
BceAI ACGGC 2 cut(s) 99, 175
BcnI CCSGG 2 cut(s) 270, 688
BcoDI GTCTC 1 cut(s) 626
BfaI CTAG 2 cut(s) 260, 365
BfoI RGCGCY 2 cut(s) 11, 165
BglI GCCNNNNNGGC 1 cut(s) 204
BisI GCNGC 6 cut(s) 11, 126, 129, 178, 288, 291
BlsI GCNGC 6 cut(s) 12, 127, 130, 179, 289, 292
Bme1390I CCNGG 2 cut(s) 270, 688
Bme18I GGWCC 1 cut(s) 416
BmgT120I GGNCC 1 cut(s) 416
BmiI GGNNCC 2 cut(s) 9, 163
BmrFI CCNGG 2 cut(s) 270, 688
BmrI ACTGGG 1 cut(s) 615
BmsI GCATC 1 cut(s) 693
BmuI ACTGGG 1 cut(s) 615
BpiI GAAGAC 1 cut(s) 243
BpuMI CCSGG 2 cut(s) 270, 688
BsaHI GRCGYC 4 cut(s) 8, 94, 162, 213
BsaJI CCNNGG 3 cut(s) 138, 202, 294
BsaXI ACNNNNNCTCC 2 cut(s) 372, 402
Bsc4I CCNNNNNNNGG 5 cut(s) 232, 275, 365, 425, 494
Bse118I RCCGGY 1 cut(s) 164
Bse1I ACTGG 3 cut(s) 402, 419, 610
BseDI CCNNGG 3 cut(s) 138, 202, 294
BseGI GGATG 5 cut(s) 50, 304, 324, 503, 544
BseLI CCNNNNNNNGG 5 cut(s) 232, 275, 365, 425, 494
BseNI ACTGG 3 cut(s) 402, 419, 610
BseRI GAGGAG 1 cut(s) 71
BseX3I CGGCCG 1 cut(s) 112
BseXI GCAGC 3 cut(s) 189, 274, 277
Bsh1236I CGCG 1 cut(s) 444
Bsh1285I CGRYCG 1 cut(s) 115
BshFI GGCC 1 cut(s) 114
BshNI GGYRCC 2 cut(s) 7, 161
BsiEI CGRYCG 1 cut(s) 115
BsiSI CCGG 4 cut(s) 111, 165, 270, 688
BslFI GGGAC 1 cut(s) 81
BslI CCNNNNNNNGG 5 cut(s) 232, 275, 365, 425, 494
BsmAI GTCTC 1 cut(s) 626
BsmFI GGGAC 1 cut(s) 81
BsmI GAATGC 3 cut(s) 193, 253, 508
BsnI GGCC 1 cut(s) 114
Bsp143I GATC 1 cut(s) 352
Bsp19I CCATGG 1 cut(s) 294
BspACI CCGC 6 cut(s) 11, 123, 126, 129, 323, 444
BspANI GGCC 1 cut(s) 114
BspFNI CGCG 1 cut(s) 444
BspLI GGNNCC 2 cut(s) 9, 163
BspPI GGATC 1 cut(s) 360
BspT107I GGYRCC 2 cut(s) 7, 161
BsrFI RCCGGY 1 cut(s) 164
BsrI ACTGG 3 cut(s) 402, 419, 610
BssAI RCCGGY 1 cut(s) 164
BssECI CCNNGG 3 cut(s) 138, 202, 294
BssMI GATC 1 cut(s) 352
BssNI GRCGYC 4 cut(s) 8, 94, 162, 213
BssT1I CCWWGG 2 cut(s) 202, 294
Bst4CI ACNGT 2 cut(s) 42, 628
Bst6I CTCTTC 2 cut(s) 28, 402
BstACI GRCGYC 4 cut(s) 8, 94, 162, 213
BstC8I GCNNGC 2 cut(s) 166, 671
BstDSI CCRYGG 2 cut(s) 138, 294
BstF5I GGATG 5 cut(s) 50, 304, 324, 503, 544
BstFNI CGCG 1 cut(s) 444
BstH2I RGCGCY 2 cut(s) 11, 165
BstHHI GCGC 2 cut(s) 10, 164
BstKTI GATC 1 cut(s) 355
BstMAI GTCTC 1 cut(s) 626
BstMBI GATC 1 cut(s) 352
BstMCI CGRYCG 1 cut(s) 115
BstMWI GCNNNNNNNGC 6 cut(s) 111, 125, 174, 204, 287, 441
BstNSI RCATGY 1 cut(s) 673
BstSCI CCNGG 2 cut(s) 268, 686
BstUI CGCG 1 cut(s) 444
BstV1I GCAGC 3 cut(s) 189, 274, 277
BstV2I GAAGAC 1 cut(s) 243
BstZI CGGCCG 1 cut(s) 112
BsuRI GGCC 1 cut(s) 114
BtgI CCRYGG 2 cut(s) 138, 294
BtsCI GGATG 5 cut(s) 50, 304, 324, 503, 544
BtsIMutI CAGTG 2 cut(s) 409, 426
Cac8I GCNNGC 2 cut(s) 166, 671
CfoI GCGC 2 cut(s) 10, 164
Cfr10I RCCGGY 1 cut(s) 164
Cfr13I GGNCC 1 cut(s) 416
CseI GACGC 2 cut(s) 43, 635
CviAII CATG 4 cut(s) 295, 557, 586, 670
CviJI RGCY 6 cut(s) 105, 114, 207, 435, 552, 584
CviKI_1 RGCY 6 cut(s) 105, 114, 207, 435, 552, 584
DinI GGCGCC 2 cut(s) 9, 163
DpnI GATC 1 cut(s) 354
DpnII GATC 1 cut(s) 352
EaeI YGGCCR 1 cut(s) 112
EagI CGGCCG 1 cut(s) 112
Eam1104I CTCTTC 2 cut(s) 28, 402
EarI CTCTTC 2 cut(s) 28, 402
EciI GGCGGA 2 cut(s) 112, 338
EclXI CGGCCG 1 cut(s) 112
Eco130I CCWWGG 2 cut(s) 202, 294
Eco47I GGWCC 1 cut(s) 416
Eco52I CGGCCG 1 cut(s) 112
EcoT14I CCWWGG 2 cut(s) 202, 294
EgeI GGCGCC 2 cut(s) 9, 163
EheI GGCGCC 2 cut(s) 9, 163
ErhI CCWWGG 2 cut(s) 202, 294
FaeI CATG 4 cut(s) 298, 560, 589, 673
FaiI YATR 6 cut(s) 296, 340, 342, 558, 587, 671
FaqI GGGAC 1 cut(s) 81
FatI CATG 4 cut(s) 294, 556, 585, 669
FblI GTMKAC 1 cut(s) 616
Fnu4HI GCNGC 6 cut(s) 11, 126, 129, 178, 288, 291
FokI GGATG 5 cut(s) 37, 311, 331, 510, 551
Fsp4HI GCNGC 6 cut(s) 11, 126, 129, 178, 288, 291
FspBI CTAG 2 cut(s) 260, 365
GlaI GCGC 2 cut(s) 9, 163
GluI GCNGC 6 cut(s) 11, 126, 129, 178, 288, 291
HaeII RGCGCY 2 cut(s) 11, 165
HaeIII GGCC 1 cut(s) 114
HapII CCGG 4 cut(s) 111, 165, 270, 688
HgaI GACGC 2 cut(s) 43, 635
HhaI GCGC 2 cut(s) 10, 164
Hin1I GRCGYC 4 cut(s) 8, 94, 162, 213
Hin1II CATG 4 cut(s) 298, 560, 589, 673
Hin6I GCGC 2 cut(s) 8, 162
HinP1I GCGC 2 cut(s) 8, 162
HindIII AAGCTT 1 cut(s) 103
HinfI GANTC 5 cut(s) 134, 145, 398, 464, 524
HpaII CCGG 4 cut(s) 111, 165, 270, 688
HphI GGTGA 1 cut(s) 154
Hpy166II GTNNAC 1 cut(s) 617
Hpy188I TCNGA 2 cut(s) 92, 246
Hpy188III TCNNGA 1 cut(s) 233
Hpy8I GTNNAC 1 cut(s) 617
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 4 cut(s) 77, 343, 418, 650
HpyCH4III ACNGT 2 cut(s) 42, 628
HpyCH4IV ACGT 3 cut(s) 94, 171, 213
HpyCH4V TGCA 4 cut(s) 589, 623, 669, 673
HpyF10VI GCNNNNNNNGC 6 cut(s) 111, 125, 174, 204, 287, 441
HpySE526I ACGT 3 cut(s) 94, 171, 213
Hsp92I GRCGYC 4 cut(s) 8, 94, 162, 213
Hsp92II CATG 4 cut(s) 298, 560, 589, 673
HspAI GCGC 2 cut(s) 8, 162
KasI GGCGCC 2 cut(s) 7, 161
KroI GCCGGC 1 cut(s) 164
KroNI GCCGGC 1 cut(s) 166
Kzo9I GATC 1 cut(s) 352
Lsp1109I GCAGC 3 cut(s) 189, 274, 277
LweI GCATC 1 cut(s) 693
MaeI CTAG 2 cut(s) 260, 365
MaeII ACGT 3 cut(s) 94, 171, 213
MaeIII GTNAC 1 cut(s) 142
MalI GATC 1 cut(s) 354
MboI GATC 1 cut(s) 352
MboII GAAGA 4 cut(s) 15, 248, 419, 523
MluCI AATT 4 cut(s) 85, 531, 636, 679
Mly113I GGCGCC 2 cut(s) 8, 162
MlyI GAGTC 3 cut(s) 139, 407, 518
MmeI TCCRAC 1 cut(s) 526
MnlI CCTC 5 cut(s) 31, 64, 92, 130, 177
MroNI GCCGGC 1 cut(s) 164
MroXI GAANNNNTTC 1 cut(s) 240
MseI TTAA 1 cut(s) 530
MslI CAYNNNNRTG 2 cut(s) 299, 590
MspI CCGG 4 cut(s) 111, 165, 270, 688
MspR9I CCNGG 2 cut(s) 270, 688
Mva1269I GAATGC 3 cut(s) 193, 253, 508
MvnI CGCG 1 cut(s) 444
MwoI GCNNNNNNNGC 6 cut(s) 111, 125, 174, 204, 287, 441
NaeI GCCGGC 1 cut(s) 166
NarI GGCGCC 2 cut(s) 8, 162
NciI CCSGG 2 cut(s) 270, 688
NcoI CCATGG 1 cut(s) 294
NdeII GATC 1 cut(s) 352
NgoMIV GCCGGC 1 cut(s) 164
NlaIII CATG 4 cut(s) 298, 560, 589, 673
NlaIV GGNNCC 2 cut(s) 9, 163
NmuCI GTSAC 1 cut(s) 142
NspI RCATGY 1 cut(s) 673
PaeI GCATGC 1 cut(s) 673
PcsI WCGNNNNNNNCGW 1 cut(s) 177
PctI GAATGC 3 cut(s) 193, 253, 508
PdiI GCCGGC 1 cut(s) 166
PdmI GAANNNNTTC 1 cut(s) 240
PfeI GAWTC 2 cut(s) 134, 464
PflMI CCANNNNNTGG 2 cut(s) 232, 494
PkrI GCNGC 6 cut(s) 12, 127, 130, 179, 289, 292
PleI GAGTC 3 cut(s) 139, 406, 518
PluTI GGCGCC 2 cut(s) 11, 165
PpsI GAGTC 3 cut(s) 139, 406, 518
PshBI ATTAAT 1 cut(s) 530
Psp1406I AACGTT 1 cut(s) 171
PspN4I GGNNCC 2 cut(s) 9, 163
PspPI GGNCC 1 cut(s) 416
RseI CAYNNNNRTG 2 cut(s) 299, 590
SaqAI TTAA 1 cut(s) 530
SatI GCNGC 6 cut(s) 11, 126, 129, 178, 288, 291
Sau3AI GATC 1 cut(s) 352
Sau96I GGNCC 1 cut(s) 416
SchI GAGTC 3 cut(s) 139, 407, 518
ScrFI CCNGG 2 cut(s) 270, 688
SetI ASST 5 cut(s) 97, 107, 174, 204, 216
SfaNI GCATC 1 cut(s) 693
SfoI GGCGCC 2 cut(s) 9, 163
SinI GGWCC 1 cut(s) 416
SmiMI CAYNNNNRTG 2 cut(s) 299, 590
SphI GCATGC 1 cut(s) 673
Sse9I AATT 4 cut(s) 85, 531, 636, 679
SsiI CCGC 6 cut(s) 11, 123, 126, 129, 323, 444
SspDI GGCGCC 2 cut(s) 7, 161
SspMI CTAG 2 cut(s) 260, 365
StyD4I CCNGG 2 cut(s) 268, 686
StyI CCWWGG 2 cut(s) 202, 294
TaaI ACNGT 2 cut(s) 42, 628
TaiI ACGT 3 cut(s) 97, 174, 216
TaqI TCGA 3 cut(s) 153, 210, 355
TasI AATT 4 cut(s) 85, 531, 636, 679
TauI GCSGC 3 cut(s) 13, 128, 131
TfiI GAWTC 2 cut(s) 134, 464
Tru1I TTAA 1 cut(s) 530
Tru9I TTAA 1 cut(s) 530
TscAI CASTG 2 cut(s) 409, 426
TseFI GTSAC 1 cut(s) 142
TseI GCWGC 3 cut(s) 177, 287, 290
Tsp45I GTSAC 1 cut(s) 142
TspDTI ATGAA 4 cut(s) 327, 456, 560, 608
TspGWI ACGGA 1 cut(s) 127
TspRI CASTG 2 cut(s) 409, 426
Van91I CCANNNNNTGG 2 cut(s) 232, 494
VpaK11BI GGWCC 1 cut(s) 416
VspI ATTAAT 1 cut(s) 530
XceI RCATGY 1 cut(s) 673
XcmI CCANNNNNNNNNTGG 1 cut(s) 495
XmiI GTMKAC 1 cut(s) 616
XmnI GAANNNNTTC 1 cut(s) 240
XspI CTAG 2 cut(s) 260, 365
ZraI GACGTC 2 cut(s) 95, 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.