pycom11g23070

Mitochondrial chaperone BCS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0024490
Physical Location & Seq
Reverse (-)
26079111 .. 26080970
1860 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1491 bp
ATGATTATTTTAGGTGAAGTTCTCCTTTCTATCCTCAAACTTGTATTTCATATCCTTCATATTTCTAAATATACACAAACACTTTGTCCTTGCAAGATGTCTGAGACAAAGGCTCTTTTGCCTGCAATAGCCACTTTCGCAGCTTCAGCAATGCTTCTTAGAAGCATTGCCAACGACTTCATCCCTAACGAATTTCGAAACTTCTTCTTCTCCGGCCTCCACAGCATTTCGCGCCGTTTCTCTTCCCACATCACCATTGTCATAGAGGAGTTCCAAGGGATGTCCTTGAATGAAGTTTTTGACGCTGTTGAAGCCTATTTGGGGACGATAGAGATCGCTTCTCTTCAAAGAATCAAAGTGAGCAAGGTTACAAACGAGAAGAAGCTAGCAGTCGCAGTGGATCGAGGGGAAGAGATAGTTGATGTTTTTGAAGACCGTGTTCAAGTAATGTGGAAATATGTTTGTACAAAGGTTGAATCTTCGCGTTCTTGTAATCCCGGCGATCTTAATGCATCCCTAAGATCAGAAACAAGATCGTACGAGCTAACATTCCACAAGAAACACAAAGAGACGGTGTTGAATTCTTACCTGCCATACATTTTAGAGAGATCAAAGGCGATCAAAGGAGAACGAAAGGTAGTAAAGCTCTGCACAAACGACCAGTATGACTGTTATTCGACACAAGAGGTCATTCTAAATCACCCCATGACCTTCGATATCCTTGCAATGGATACGGAGCTTAAGAAAGCTTTGTTGGATGATTTGAACAACTTCAAAAATGGCAAGGAATTTTACAGAAGGATAGGAAAAGCTTGGAAACGTGGATACTTGTTGTATGGCCCTCCTGGCACAGGCAAGTCCAGCTTGATTGCAGCCATTGCTAACCACCTTAACTACGACATCTACGAGTTGGAGCTAACCGATGCTGGTTCCAATTCTGATTTGAAGAGAAGGTTGCTAGCCATGCCTGACAAATCTATTATCGTAATCGAAGACATTGATTGCACAATCAAGCTGCAAAATCGGGAATCTGAGGAGGAATCGTGTAATACCAACAGAAATCAGGTGACACTCTCCGGACTCCTCAACCTCATTGATGGTCTTTGGTCATGTTGTACTGATGAACGCATTATAATTTTCACAACAAATCATAAAGACAAACTAGATCCTGCTCTAGTGAGACCTGGCCGAATGGACATGCACATTCACATGTCGTATTGCACCATTTCTGCATTCAAGCAGCTGGCATTCAATTACCATGGAGTTCGCAATGACCAACTCTTCGAACAGATCGAAGGACTTATATTGGAGGTTGAAGTCACCCCAGCAGAAGTTGCAGGAGAACTAATGAGGAGCAGAGATGCTCAAATTTCGCTCCGACGCCTCATCGATTTCTTGCTGGAGAAGAAAACTCAACAAGATCACAAGGCTAAAACTAATGAGACCGGTACGAAAGAAGAGCACAACATGGAAGAGGAGCAGACTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA AAA_assoc

Protein Analysis

497

Amino Acids

56.66

Weight (kDa)

6.11

Isoelectric Point (pI)

53.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 61 - 155 2.1e-21 Domain associated at C-terminal with AAA
AAA PF00004 276 - 405 3.9e-21 ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540 PF25568 407 - 476 3.5e-21 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024490)

Species Orthologous Gene IDs
malus_domestica MD11G1261500.v1.1
prunus_persica Prupe.4G166800_v2.0.a1
pyrus_communis pycom11g23070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1133
Acc36I ACCTGC 1 cut(s) 597
AccII CGCG 2 cut(s) 232, 484
AccIII TCCGGA 1 cut(s) 1076
AclWI GGATC 2 cut(s) 408, 1160
AcoI YGGCCR 1 cut(s) 1186
AcsI RAATTY 4 cut(s) 191, 580, 788, 1368
AcuI CTGAAG 1 cut(s) 129
AcyI GRCGYC 1 cut(s) 1381
AfaI GTAC 4 cut(s) 466, 539, 1117, 1450
AfiI CCNNNNNNNGG 3 cut(s) 321, 727, 851
AflII CTTAAG 1 cut(s) 740
AflIII ACRYGT 1 cut(s) 1209
AgeI ACCGGT 1 cut(s) 1445
AhlI ACTAGT 1 cut(s) 1484
AjnI CCWGG 2 cut(s) 844, 1183
AjuI GAANNNNNNNTTGG 2 cut(s) 737, 769
Alw21I GWGCWC 1 cut(s) 1464
Alw26I GTCTC 4 cut(s) 98, 563, 1174, 1436
AlwI GGATC 2 cut(s) 408, 1160
Aor13HI TCCGGA 1 cut(s) 1076
AoxI GGCC 3 cut(s) 214, 838, 1186
ApeKI GCWGC 4 cut(s) 140, 872, 1015, 1240
ApoI RAATTY 4 cut(s) 191, 580, 788, 1368
ArsI GACNNNNNNTTYG 4 cut(s) 70, 102, 1265, 1297
AsiGI ACCGGT 1 cut(s) 1445
Asp700I GAANNNNTTC 1 cut(s) 770
AspLEI GCGC 1 cut(s) 234
AspS9I GGNCC 1 cut(s) 839
AsuC2I CCSGG 1 cut(s) 498
AsuHPI GGTGA 5 cut(s) 26, 244, 692, 1078, 1312
AsuII TTCGAA 2 cut(s) 196, 1284
AsuNHI GCTAGC 2 cut(s) 385, 958
BbsI GAAGAC 2 cut(s) 438, 999
Bbv12I GWGCWC 1 cut(s) 1464
BbvI GCAGC 4 cut(s) 152, 884, 1002, 1252
BccI CCATC 1 cut(s) 1091
BceAI ACGGC 1 cut(s) 219
BcgI CGANNNNNNTGC 4 cut(s) 491, 525, 704, 738
BciT130I CCWGG 2 cut(s) 846, 1185
BciVI GTATCC 2 cut(s) 724, 818
BcnI CCSGG 1 cut(s) 498
BcoDI GTCTC 4 cut(s) 98, 563, 1174, 1436
BcuI ACTAGT 1 cut(s) 1484
BfaI CTAG 5 cut(s) 386, 959, 1163, 1175, 1485
BfrI CTTAAG 1 cut(s) 740
BfuAI ACCTGC 1 cut(s) 597
BfuI GTATCC 2 cut(s) 724, 818
BglI GCCNNNNNGGC 1 cut(s) 846
BisI GCNGC 4 cut(s) 141, 873, 1016, 1241
BlsI GCNGC 4 cut(s) 142, 874, 1017, 1242
Bme1390I CCNGG 3 cut(s) 498, 846, 1185
BmgT120I GGNCC 1 cut(s) 839
BmiI GGNNCC 1 cut(s) 931
BmrFI CCNGG 3 cut(s) 498, 846, 1185
BmsI GCATC 3 cut(s) 521, 913, 1351
BmtI GCTAGC 2 cut(s) 389, 962
BpiI GAAGAC 2 cut(s) 438, 999
BpmI CTGGAG 1 cut(s) 1421
Bpu14I TTCGAA 2 cut(s) 196, 1284
BpuMI CCSGG 1 cut(s) 498
Bsa29I ATCGAT 1 cut(s) 1389
BsaBI GATNNNNATC 1 cut(s) 332
BsaHI GRCGYC 1 cut(s) 1381
BsaI GGTCTC 2 cut(s) 1174, 1436
BsaJI CCNNGG 2 cut(s) 274, 1258
BsaWI WCCGGW 2 cut(s) 1076, 1445
BsaXI ACNNNNNCTCC 2 cut(s) 1028, 1058
Bsc4I CCNNNNNNNGG 3 cut(s) 321, 727, 851
Bse118I RCCGGY 1 cut(s) 1445
Bse1I ACTGG 1 cut(s) 661
Bse3DI GCAATG 5 cut(s) 156, 165, 732, 876, 1276
Bse8I GATNNNNATC 1 cut(s) 332
BseAI TCCGGA 1 cut(s) 1076
BseBI CCWGG 2 cut(s) 846, 1185
BseCI ATCGAT 1 cut(s) 1389
BseDI CCNNGG 2 cut(s) 274, 1258
BseGI GGATG 4 cut(s) 180, 285, 512, 763
BseJI GATNNNNATC 1 cut(s) 332
BseLI CCNNNNNNNGG 3 cut(s) 321, 727, 851
BseMI GCAATG 5 cut(s) 156, 165, 732, 876, 1276
BseMII CTCAG 2 cut(s) 93, 1023
BseNI ACTGG 1 cut(s) 661
BseRI GAGGAG 5 cut(s) 281, 1049, 1073, 1366, 1490
BseXI GCAGC 4 cut(s) 152, 884, 1002, 1252
BseYI CCCAGC 1 cut(s) 1324
BsgI GTGCAG 1 cut(s) 634
Bsh1236I CGCG 2 cut(s) 232, 484
BshFI GGCC 3 cut(s) 216, 840, 1188
BshTI ACCGGT 1 cut(s) 1445
BshVI ATCGAT 1 cut(s) 1389
BsiHKAI GWGCWC 1 cut(s) 1464
BsiSI CCGG 4 cut(s) 213, 498, 1077, 1446
BsiWI CGTACG 1 cut(s) 537
BslFI GGGAC 1 cut(s) 337
BslI CCNNNNNNNGG 3 cut(s) 321, 727, 851
BsmAI GTCTC 4 cut(s) 98, 563, 1174, 1436
BsmBI CGTCTC 1 cut(s) 563
BsmFI GGGAC 1 cut(s) 337
BsmI GAATGC 2 cut(s) 1232, 1247
BsnI GGCC 3 cut(s) 216, 840, 1188
Bso31I GGTCTC 2 cut(s) 1174, 1436
Bsp119I TTCGAA 2 cut(s) 196, 1284
Bsp1286I GDGCHC 1 cut(s) 1464
Bsp13I TCCGGA 1 cut(s) 1076
Bsp1407I TGTACA 1 cut(s) 464
Bsp19I CCATGG 1 cut(s) 1258
BspANI GGCC 3 cut(s) 216, 840, 1188
BspCNI CTCAG 2 cut(s) 94, 1024
BspDI ATCGAT 1 cut(s) 1389
BspEI TCCGGA 1 cut(s) 1076
BspFNI CGCG 2 cut(s) 232, 484
BspLI GGNNCC 1 cut(s) 931
BspMI ACCTGC 1 cut(s) 597
BspOI GCTAGC 2 cut(s) 389, 962
BspPI GGATC 2 cut(s) 408, 1160
BspQI GCTCTTC 1 cut(s) 1452
BspT104I TTCGAA 2 cut(s) 196, 1284
BspTI CTTAAG 1 cut(s) 740
BspTNI GGTCTC 2 cut(s) 1174, 1436
BsrDI GCAATG 5 cut(s) 156, 165, 732, 876, 1276
BsrFI RCCGGY 1 cut(s) 1445
BsrGI TGTACA 1 cut(s) 464
BsrI ACTGG 1 cut(s) 661
BssAI RCCGGY 1 cut(s) 1445
BssECI CCNNGG 2 cut(s) 274, 1258
BssNI GRCGYC 1 cut(s) 1381
BssT1I CCWWGG 2 cut(s) 274, 1258
Bst2UI CCWGG 2 cut(s) 846, 1185
Bst4CI ACNGT 3 cut(s) 437, 574, 671
Bst6I CTCTTC 7 cut(s) 247, 348, 405, 941, 1286, 1452, 1467
BstACI GRCGYC 1 cut(s) 1381
BstAFI CTTAAG 1 cut(s) 740
BstAPI GCANNNNNTGC 2 cut(s) 878, 1334
BstAUI TGTACA 1 cut(s) 464
BstBI TTCGAA 2 cut(s) 196, 1284
BstC8I GCNNGC 4 cut(s) 123, 387, 960, 1245
BstDEI CTNAG 4 cut(s) 102, 158, 518, 1032
BstDSI CCRYGG 1 cut(s) 1258
BstENI CCTNNNNNAGG 1 cut(s) 849
BstF5I GGATG 4 cut(s) 180, 285, 512, 763
BstFNI CGCG 2 cut(s) 232, 484
BstHHI GCGC 1 cut(s) 234
BstMAI GTCTC 4 cut(s) 98, 563, 1174, 1436
BstNI CCWGG 2 cut(s) 846, 1185
BstNSI RCATGY 2 cut(s) 1201, 1213
BstSCI CCNGG 3 cut(s) 496, 844, 1183
BstUI CGCG 2 cut(s) 232, 484
BstV1I GCAGC 4 cut(s) 152, 884, 1002, 1252
BstV2I GAAGAC 2 cut(s) 438, 999
BstX2I RGATCY 1 cut(s) 1165
BstYI RGATCY 1 cut(s) 1165
Bsu15I ATCGAT 1 cut(s) 1389
BsuI GTATCC 2 cut(s) 724, 818
BsuRI GGCC 3 cut(s) 216, 840, 1188
BsuTUI ATCGAT 1 cut(s) 1389
BtgI CCRYGG 1 cut(s) 1258
BtsCI GGATG 4 cut(s) 180, 285, 512, 763
BtsI GCAGTG 1 cut(s) 402
BtsIMutI CAGTG 1 cut(s) 402
BveI ACCTGC 1 cut(s) 597
Cac8I GCNNGC 4 cut(s) 123, 387, 960, 1245
CfoI GCGC 1 cut(s) 234
Cfr10I RCCGGY 1 cut(s) 1445
Cfr13I GGNCC 1 cut(s) 839
ClaI ATCGAT 1 cut(s) 1389
CseI GACGC 2 cut(s) 311, 1389
Csp6I GTAC 4 cut(s) 465, 538, 1116, 1449
CspAI ACCGGT 1 cut(s) 1445
CspCI CAANNNNNGTGG 2 cut(s) 431, 466
CviAII CATG 7 cut(s) 706, 964, 1110, 1198, 1210, 1259, 1468
CviQI GTAC 4 cut(s) 465, 538, 1116, 1449
DdeI CTNAG 4 cut(s) 102, 158, 518, 1032
EaeI YGGCCR 1 cut(s) 1186
Eam1104I CTCTTC 7 cut(s) 247, 348, 405, 941, 1286, 1452, 1467
EarI CTCTTC 7 cut(s) 247, 348, 405, 941, 1286, 1452, 1467
Eco130I CCWWGG 2 cut(s) 274, 1258
Eco31I GGTCTC 2 cut(s) 1174, 1436
Eco32I GATATC 1 cut(s) 718
Eco57I CTGAAG 1 cut(s) 129
EcoNI CCTNNNNNAGG 1 cut(s) 849
EcoRI GAATTC 1 cut(s) 580
EcoRII CCWGG 2 cut(s) 844, 1183
EcoRV GATATC 1 cut(s) 718
EcoT14I CCWWGG 2 cut(s) 274, 1258
EcoT22I ATGCAT 1 cut(s) 514
ErhI CCWWGG 2 cut(s) 274, 1258
Esp3I CGTCTC 1 cut(s) 563
FaeI CATG 7 cut(s) 709, 967, 1113, 1201, 1213, 1262, 1471
FalI AAGNNNNNCTT 4 cut(s) 9, 41, 848, 880
FaqI GGGAC 1 cut(s) 337
FatI CATG 7 cut(s) 705, 963, 1109, 1197, 1209, 1258, 1467
Fnu4HI GCNGC 4 cut(s) 141, 873, 1016, 1241
FokI GGATG 4 cut(s) 167, 292, 499, 770
Fsp4HI GCNGC 4 cut(s) 141, 873, 1016, 1241
FspBI CTAG 5 cut(s) 386, 959, 1163, 1175, 1485
GlaI GCGC 1 cut(s) 233
GluI GCNGC 4 cut(s) 141, 873, 1016, 1241
GsaI CCCAGC 1 cut(s) 1328
GsuI CTGGAG 1 cut(s) 1421
HaeIII GGCC 3 cut(s) 216, 840, 1188
HapII CCGG 4 cut(s) 213, 498, 1077, 1446
HgaI GACGC 2 cut(s) 311, 1389
HhaI GCGC 1 cut(s) 234
Hin1I GRCGYC 1 cut(s) 1381
Hin1II CATG 7 cut(s) 709, 967, 1113, 1201, 1213, 1262, 1471
Hin6I GCGC 1 cut(s) 232
HinP1I GCGC 1 cut(s) 232
HindIII AAGCTT 2 cut(s) 747, 810
HinfI GANTC 5 cut(s) 351, 476, 1028, 1040, 1080
HpaII CCGG 4 cut(s) 213, 498, 1077, 1446
HphI GGTGA 5 cut(s) 26, 244, 692, 1078, 1312
Hpy188I TCNGA 5 cut(s) 103, 526, 940, 1033, 1379
Hpy188III TCNNGA 2 cut(s) 1025, 1077
Hpy99I CGWCG 1 cut(s) 1383
HpyAV CCTTC 5 cut(s) 65, 721, 792, 945, 1289
HpyCH4III ACNGT 3 cut(s) 437, 574, 671
HpyCH4IV ACGT 1 cut(s) 820
HpyF3I CTNAG 4 cut(s) 102, 158, 518, 1032
HpySE526I ACGT 1 cut(s) 820
Hsp92I GRCGYC 1 cut(s) 1381
Hsp92II CATG 7 cut(s) 709, 967, 1113, 1201, 1213, 1262, 1471
HspAI GCGC 1 cut(s) 232
Kpn2I TCCGGA 1 cut(s) 1076
LguI GCTCTTC 1 cut(s) 1452
LmnI GCTCC 5 cut(s) 736, 913, 1353, 1380, 1477
Lsp1109I GCAGC 4 cut(s) 152, 884, 1002, 1252
LweI GCATC 3 cut(s) 521, 913, 1351
MaeI CTAG 5 cut(s) 386, 959, 1163, 1175, 1485
MaeII ACGT 1 cut(s) 820
MaeIII GTNAC 3 cut(s) 367, 1066, 1318
MflI RGATCY 1 cut(s) 1165
MhlI GDGCHC 1 cut(s) 1464
MluCI AATT 7 cut(s) 191, 580, 788, 934, 1134, 1252, 1368
MlyI GAGTC 1 cut(s) 1074
MmeI TCCRAC 3 cut(s) 735, 891, 1402
Mph1103I ATGCAT 1 cut(s) 514
MroI TCCGGA 1 cut(s) 1076
MroXI GAANNNNTTC 1 cut(s) 770
MseI TTAA 3 cut(s) 507, 741, 891
MslI CAYNNNNRTG 1 cut(s) 1208
MspA1I CMGCKG 1 cut(s) 1243
MspCI CTTAAG 1 cut(s) 740
MspI CCGG 4 cut(s) 213, 498, 1077, 1446
MspR9I CCNGG 3 cut(s) 498, 846, 1185
Mva1269I GAATGC 2 cut(s) 1232, 1247
MvaI CCWGG 2 cut(s) 846, 1185
MvnI CGCG 2 cut(s) 232, 484
NciI CCSGG 1 cut(s) 498
NcoI CCATGG 1 cut(s) 1258
NheI GCTAGC 2 cut(s) 385, 958
NlaIII CATG 7 cut(s) 709, 967, 1113, 1201, 1213, 1262, 1471
NlaIV GGNNCC 1 cut(s) 931
NmuCI GTSAC 2 cut(s) 1066, 1318
NsiI ATGCAT 1 cut(s) 514
NspI RCATGY 2 cut(s) 1201, 1213
NspV TTCGAA 2 cut(s) 196, 1284
PciI ACATGT 1 cut(s) 1209
PciSI GCTCTTC 1 cut(s) 1452
PcsI WCGNNNNNNNCGW 2 cut(s) 903, 1290
PctI GAATGC 2 cut(s) 1232, 1247
PdmI GAANNNNTTC 1 cut(s) 770
PfeI GAWTC 4 cut(s) 351, 476, 1028, 1040
Pfl23II CGTACG 1 cut(s) 537
PinAI ACCGGT 1 cut(s) 1445
PkrI GCNGC 4 cut(s) 142, 874, 1017, 1242
PleI GAGTC 1 cut(s) 1074
PpsI GAGTC 1 cut(s) 1074
PscI ACATGT 1 cut(s) 1209
PsiI TTATAA 1 cut(s) 1133
Psp6I CCWGG 2 cut(s) 844, 1183
PspFI CCCAGC 1 cut(s) 1324
PspGI CCWGG 2 cut(s) 844, 1183
PspLI CGTACG 1 cut(s) 537
PspN4I GGNNCC 1 cut(s) 931
PspPI GGNCC 1 cut(s) 839
PsuI RGATCY 1 cut(s) 1165
PvuII CAGCTG 1 cut(s) 1243
RsaI GTAC 4 cut(s) 466, 539, 1117, 1450
RsaNI GTAC 4 cut(s) 465, 538, 1116, 1449
RseI CAYNNNNRTG 1 cut(s) 1208
SapI GCTCTTC 1 cut(s) 1452
SaqAI TTAA 3 cut(s) 507, 741, 891
SatI GCNGC 4 cut(s) 141, 873, 1016, 1241
Sau96I GGNCC 1 cut(s) 839
SchI GAGTC 1 cut(s) 1074
ScrFI CCNGG 3 cut(s) 498, 846, 1185
SduI GDGCHC 1 cut(s) 1464
SfaNI GCATC 3 cut(s) 521, 913, 1351
SfuI TTCGAA 2 cut(s) 196, 1284
SmiMI CAYNNNNRTG 1 cut(s) 1208
SmlI CTYRAG 1 cut(s) 740
SmoI CTYRAG 1 cut(s) 740
SpeI ACTAGT 1 cut(s) 1484
Sse9I AATT 7 cut(s) 191, 580, 788, 934, 1134, 1252, 1368
SspMI CTAG 5 cut(s) 386, 959, 1163, 1175, 1485
StyD4I CCNGG 3 cut(s) 496, 844, 1183
StyI CCWWGG 2 cut(s) 274, 1258
TaaI ACNGT 3 cut(s) 437, 574, 671
TaiI ACGT 1 cut(s) 823
TaqI TCGA 8 cut(s) 196, 403, 677, 714, 990, 1284, 1293, 1389
TasI AATT 7 cut(s) 191, 580, 788, 934, 1134, 1252, 1368
TatI WGTACW 2 cut(s) 464, 1115
TfiI GAWTC 4 cut(s) 351, 476, 1028, 1040
Tru1I TTAA 3 cut(s) 507, 741, 891
Tru9I TTAA 3 cut(s) 507, 741, 891
TscAI CASTG 1 cut(s) 402
TseFI GTSAC 2 cut(s) 1066, 1318
TseI GCWGC 4 cut(s) 140, 872, 1015, 1240
Tsp45I GTSAC 2 cut(s) 1066, 1318
TspDTI ATGAA 5 cut(s) 38, 47, 169, 306, 1137
TspGWI ACGGA 1 cut(s) 749
TspRI CASTG 1 cut(s) 402
Vha464I CTTAAG 1 cut(s) 740
XagI CCTNNNNNAGG 1 cut(s) 849
XapI RAATTY 4 cut(s) 191, 580, 788, 1368
XceI RCATGY 2 cut(s) 1201, 1213
XmnI GAANNNNTTC 1 cut(s) 770
XspI CTAG 5 cut(s) 386, 959, 1163, 1175, 1485
Zsp2I ATGCAT 1 cut(s) 514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.