pycom126g00070

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000126
OG0028679
Physical Location & Seq
Forward (+)
45672 .. 46373
702 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 487 bp
ATGGTGGGGTCAGTGGCGAATCCCAGGCTTCGCAGCCTGCTATGGTGGCGAAGATGGGCCAAAAAGGACTGGGCAATCGCCGCCGTCGGATTTACCGTTATCGCCTTCGCTCTCACTCTCCTCTCCAACTCTTGGCGCGACGAACCAGACTGCGAAATCTCCCGCCCGTTTCTTCCCAACGCCGTCGCCTTCGATTTCGTTGACTTGACCTTGCTGCGCAACGCCAAAGATAGAGGCGCCCTTTGTTTGGACGGGACTGCCCCTGGCTACCATTTCAGGAAGGGATTCGGATCCGGCGCCAACAATTGGCTGCTTCACATTGAGGTCAAAATTCTATCCCTGTGCCTTTTTTTACAGGGTGGAGGTTGGTGCAATTCGATTGAATCATGTTCTTGGCGCAAAGGTACTCTACTAGGGTCTTCTAATTACATGGATCGTCGAGTTCCCTTTTTTGGGATTTTGAGCTCTCATCCATCACAAAATCCTG

Protein Analysis

162

Amino Acids

18.13

Weight (kDa)

9.36

Isoelectric Point (pI)

55.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 66 - 162 6.6e-30 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0028679)

Species Orthologous Gene IDs
malus_domestica MD04G1127700.v1.1
pyrus_communis pycom126g00070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 218
AccB1I GGYRCC 2 cut(s) 236, 296
AccB7I CCANNNNNTGG 2 cut(s) 132, 306
AccII CGCG 1 cut(s) 138
AciI CCGC 2 cut(s) 81, 163
AclWI GGATC 3 cut(s) 285, 298, 441
AcsI RAATTY 1 cut(s) 330
AcyI GRCGYC 2 cut(s) 237, 297
AfaI GTAC 1 cut(s) 406
AfiI CCNNNNNNNGG 5 cut(s) 132, 247, 306, 452, 453
AgsI TTSAA 1 cut(s) 383
AjnI CCWGG 2 cut(s) 23, 262
AluBI AGCT 1 cut(s) 465
AluI AGCT 1 cut(s) 465
Alw21I GWGCWC 1 cut(s) 467
AlwI GGATC 3 cut(s) 285, 298, 441
AoxI GGCC 1 cut(s) 57
ApeKI GCWGC 3 cut(s) 33, 214, 310
ApoI RAATTY 1 cut(s) 330
Asp700I GAANNNNTTC 1 cut(s) 284
AspLEI GCGC 5 cut(s) 138, 219, 239, 299, 399
AspS9I GGNCC 1 cut(s) 57
BamHI GGATCC 1 cut(s) 290
BanI GGYRCC 2 cut(s) 236, 296
BanII GRGCYC 1 cut(s) 467
BbsI GAAGAC 1 cut(s) 411
Bbv12I GWGCWC 1 cut(s) 467
BbvI GCAGC 3 cut(s) 45, 201, 297
BccI CCATC 2 cut(s) 48, 481
BceAI ACGGC 2 cut(s) 68, 167
BciT130I CCWGG 2 cut(s) 25, 264
BfaI CTAG 1 cut(s) 413
BfoI RGCGCY 2 cut(s) 240, 300
BisI GCNGC 4 cut(s) 34, 81, 215, 311
BlsI GCNGC 4 cut(s) 35, 82, 216, 312
Bme1390I CCNGG 2 cut(s) 25, 264
BmgT120I GGNCC 1 cut(s) 57
BmiI GGNNCC 3 cut(s) 238, 292, 298
BmrFI CCNGG 2 cut(s) 25, 264
BmrI ACTGGG 1 cut(s) 79
BmuI ACTGGG 1 cut(s) 79
BpiI GAAGAC 1 cut(s) 411
BsaBI GATNNNNATC 1 cut(s) 289
BsaHI GRCGYC 2 cut(s) 237, 297
BsaJI CCNNGG 2 cut(s) 23, 262
Bsc4I CCNNNNNNNGG 5 cut(s) 132, 247, 306, 452, 453
Bse1I ACTGG 1 cut(s) 74
Bse8I GATNNNNATC 1 cut(s) 289
BseBI CCWGG 2 cut(s) 25, 264
BseDI CCNNGG 2 cut(s) 23, 262
BseGI GGATG 1 cut(s) 469
BseJI GATNNNNATC 1 cut(s) 289
BseLI CCNNNNNNNGG 5 cut(s) 132, 247, 306, 452, 453
BseNI ACTGG 1 cut(s) 74
BseRI GAGGAG 1 cut(s) 110
BseXI GCAGC 3 cut(s) 45, 201, 297
Bsh1236I CGCG 1 cut(s) 138
BshFI GGCC 1 cut(s) 59
BshNI GGYRCC 2 cut(s) 236, 296
BsiHKAI GWGCWC 1 cut(s) 467
BsiSI CCGG 1 cut(s) 294
BslFI GGGAC 1 cut(s) 268
BslI CCNNNNNNNGG 5 cut(s) 132, 247, 306, 452, 453
BsmFI GGGAC 1 cut(s) 268
BsnI GGCC 1 cut(s) 59
Bsp1286I GDGCHC 1 cut(s) 467
Bsp143I GATC 2 cut(s) 290, 433
BspACI CCGC 2 cut(s) 81, 163
BspANI GGCC 1 cut(s) 59
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 3 cut(s) 238, 292, 298
BspPI GGATC 3 cut(s) 285, 298, 441
BspT107I GGYRCC 2 cut(s) 236, 296
BsrI ACTGG 1 cut(s) 74
BssECI CCNNGG 2 cut(s) 23, 262
BssMI GATC 2 cut(s) 290, 433
BssNI GRCGYC 2 cut(s) 237, 297
Bst2UI CCWGG 2 cut(s) 25, 264
Bst4CI ACNGT 1 cut(s) 97
BstACI GRCGYC 2 cut(s) 237, 297
BstC8I GCNNGC 1 cut(s) 38
BstF5I GGATG 1 cut(s) 469
BstFNI CGCG 1 cut(s) 138
BstH2I RGCGCY 2 cut(s) 240, 300
BstHHI GCGC 5 cut(s) 138, 219, 239, 299, 399
BstKTI GATC 2 cut(s) 293, 436
BstMBI GATC 2 cut(s) 290, 433
BstMWI GCNNNNNNNGC 2 cut(s) 46, 80
BstNI CCWGG 2 cut(s) 25, 264
BstSCI CCNGG 2 cut(s) 23, 262
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 3 cut(s) 45, 201, 297
BstV2I GAAGAC 1 cut(s) 411
BstX2I RGATCY 1 cut(s) 290
BstYI RGATCY 1 cut(s) 290
BsuRI GGCC 1 cut(s) 59
BtsCI GGATG 1 cut(s) 469
BtsIMutI CAGTG 1 cut(s) 18
Cac8I GCNNGC 1 cut(s) 38
CfoI GCGC 5 cut(s) 138, 219, 239, 299, 399
Cfr13I GGNCC 1 cut(s) 57
Csp6I GTAC 1 cut(s) 405
CviAII CATG 2 cut(s) 387, 430
CviJI RGCY 6 cut(s) 28, 36, 59, 267, 310, 465
CviKI_1 RGCY 6 cut(s) 28, 36, 59, 267, 310, 465
CviQI GTAC 1 cut(s) 405
DinI GGCGCC 2 cut(s) 238, 298
DpnI GATC 2 cut(s) 292, 435
DpnII GATC 2 cut(s) 290, 433
Ecl136II GAGCTC 1 cut(s) 465
Eco24I GRGCYC 1 cut(s) 467
Eco53kI GAGCTC 1 cut(s) 465
EcoICRI GAGCTC 1 cut(s) 465
EcoRII CCWGG 2 cut(s) 23, 262
EcoT38I GRGCYC 1 cut(s) 467
EgeI GGCGCC 2 cut(s) 238, 298
EheI GGCGCC 2 cut(s) 238, 298
FaeI CATG 2 cut(s) 390, 433
FaiI YATR 3 cut(s) 43, 388, 431
FaqI GGGAC 1 cut(s) 268
FatI CATG 2 cut(s) 386, 429
FauI CCCGC 1 cut(s) 170
Fnu4HI GCNGC 4 cut(s) 34, 81, 215, 311
FokI GGATG 1 cut(s) 456
FriOI GRGCYC 1 cut(s) 467
Fsp4HI GCNGC 4 cut(s) 34, 81, 215, 311
FspBI CTAG 1 cut(s) 413
FspI TGCGCA 1 cut(s) 218
GlaI GCGC 5 cut(s) 137, 218, 238, 298, 398
GluI GCNGC 4 cut(s) 34, 81, 215, 311
HaeII RGCGCY 2 cut(s) 240, 300
HaeIII GGCC 1 cut(s) 59
HapII CCGG 1 cut(s) 294
HhaI GCGC 5 cut(s) 138, 219, 239, 299, 399
Hin1I GRCGYC 2 cut(s) 237, 297
Hin1II CATG 2 cut(s) 390, 433
Hin6I GCGC 5 cut(s) 136, 217, 237, 297, 397
HinP1I GCGC 5 cut(s) 136, 217, 237, 297, 397
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 3 cut(s) 19, 285, 383
HpaII CCGG 1 cut(s) 294
Hpy166II GTNNAC 1 cut(s) 202
Hpy188I TCNGA 2 cut(s) 89, 290
Hpy188III TCNNGA 1 cut(s) 277
Hpy8I GTNNAC 1 cut(s) 202
Hpy99I CGWCG 4 cut(s) 89, 143, 188, 441
HpyAV CCTTC 3 cut(s) 115, 199, 274
HpyCH4III ACNGT 1 cut(s) 97
HpyCH4V TGCA 1 cut(s) 372
HpyF10VI GCNNNNNNNGC 2 cut(s) 46, 80
Hsp92I GRCGYC 2 cut(s) 237, 297
Hsp92II CATG 2 cut(s) 390, 433
HspAI GCGC 5 cut(s) 136, 217, 237, 297, 397
KasI GGCGCC 2 cut(s) 236, 296
Kzo9I GATC 2 cut(s) 290, 433
Lsp1109I GCAGC 3 cut(s) 45, 201, 297
MaeI CTAG 1 cut(s) 413
MalI GATC 2 cut(s) 292, 435
MboI GATC 2 cut(s) 290, 433
MboII GAAGA 3 cut(s) 63, 164, 411
MfeI CAATTG 1 cut(s) 304
MflI RGATCY 1 cut(s) 290
MhlI GDGCHC 1 cut(s) 467
MluCI AATT 4 cut(s) 304, 330, 373, 424
Mly113I GGCGCC 2 cut(s) 237, 297
MmeI TCCRAC 2 cut(s) 67, 150
MnlI CCTC 4 cut(s) 131, 227, 316, 356
MroXI GAANNNNTTC 1 cut(s) 284
MspI CCGG 1 cut(s) 294
MspR9I CCNGG 2 cut(s) 25, 264
MunI CAATTG 1 cut(s) 304
MvaI CCWGG 2 cut(s) 25, 264
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 2 cut(s) 46, 80
NarI GGCGCC 2 cut(s) 237, 297
NdeII GATC 2 cut(s) 290, 433
NlaIII CATG 2 cut(s) 390, 433
NlaIV GGNNCC 3 cut(s) 238, 292, 298
NsbI TGCGCA 1 cut(s) 218
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PdmI GAANNNNTTC 1 cut(s) 284
PfeI GAWTC 3 cut(s) 19, 285, 383
PflMI CCANNNNNTGG 2 cut(s) 132, 306
PkrI GCNGC 4 cut(s) 35, 82, 216, 312
PluTI GGCGCC 2 cut(s) 240, 300
Psp124BI GAGCTC 1 cut(s) 467
Psp6I CCWGG 2 cut(s) 23, 262
PspGI CCWGG 2 cut(s) 23, 262
PspN4I GGNNCC 3 cut(s) 238, 292, 298
PspPI GGNCC 1 cut(s) 57
PsuI RGATCY 1 cut(s) 290
RsaI GTAC 1 cut(s) 406
RsaNI GTAC 1 cut(s) 405
SacI GAGCTC 1 cut(s) 467
SatI GCNGC 4 cut(s) 34, 81, 215, 311
Sau3AI GATC 2 cut(s) 290, 433
Sau96I GGNCC 1 cut(s) 57
ScrFI CCNGG 2 cut(s) 25, 264
SduI GDGCHC 1 cut(s) 467
SetI ASST 5 cut(s) 212, 327, 367, 406, 467
SfoI GGCGCC 2 cut(s) 238, 298
Sse9I AATT 4 cut(s) 304, 330, 373, 424
SsiI CCGC 2 cut(s) 81, 163
SspDI GGCGCC 2 cut(s) 236, 296
SspMI CTAG 1 cut(s) 413
SstI GAGCTC 1 cut(s) 467
StyD4I CCNGG 2 cut(s) 23, 262
TaaI ACNGT 1 cut(s) 97
TaqI TCGA 3 cut(s) 192, 377, 439
TasI AATT 4 cut(s) 304, 330, 373, 424
TauI GCSGC 1 cut(s) 83
TfiI GAWTC 3 cut(s) 19, 285, 383
TscAI CASTG 1 cut(s) 18
TseI GCWGC 3 cut(s) 33, 214, 310
TspRI CASTG 1 cut(s) 18
Van91I CCANNNNNTGG 2 cut(s) 132, 306
XapI RAATTY 1 cut(s) 330
XmnI GAANNNNTTC 1 cut(s) 284
XspI CTAG 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.