pycom12g02450

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0028710
Physical Location & Seq
Reverse (-)
2149338 .. 2149616
279 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 279 bp
ATGTTATTTACAGATTTTGACGAAATGGTACACTCAATCATGATGAGAGGAAAGATTGTGGTTTGTATCATGGATAAAATCAACGACGATGTGGTGGAGAAAGCAAAGGTCGTAAAAGAAGGCGGAGCCGTTGGAATGATTCTCATCGATCAATTTAATAACTTCAACGAGCAGCTTCCTTCTTTTGATCTCCCTACTTCGGTAATTAGTAGCGTGGAAGGACGCAAACTTTCCGATTATATGGCTACGAGAAGGTTAACATTTCATTCATATTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

93

Amino Acids

10.48

Weight (kDa)

5.2

Isoelectric Point (pI)

26.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PA PF02225 14 - 77 4.2e-08 PA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0028710)

Species Orthologous Gene IDs
malus_domestica MD12G1025000.v1.1
pyrus_communis pycom12g02450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 123
AfaI GTAC 1 cut(s) 30
AfiI CCNNNNNNNGG 1 cut(s) 199
AgsI TTSAA 1 cut(s) 166
AluBI AGCT 1 cut(s) 175
AluI AGCT 1 cut(s) 175
ApeKI GCWGC 1 cut(s) 172
BbvI GCAGC 1 cut(s) 184
BceAI ACGGC 1 cut(s) 113
BisI GCNGC 1 cut(s) 173
BlsI GCNGC 1 cut(s) 174
BmiI GGNNCC 1 cut(s) 127
Bsa29I ATCGAT 1 cut(s) 147
BsaBI GATNNNNATC 1 cut(s) 143
Bsc4I CCNNNNNNNGG 1 cut(s) 199
Bse8I GATNNNNATC 1 cut(s) 143
BseCI ATCGAT 1 cut(s) 147
BseJI GATNNNNATC 1 cut(s) 143
BseLI CCNNNNNNNGG 1 cut(s) 199
BseXI GCAGC 1 cut(s) 184
BshVI ATCGAT 1 cut(s) 147
BslI CCNNNNNNNGG 1 cut(s) 199
Bsp143I GATC 2 cut(s) 148, 187
BspACI CCGC 1 cut(s) 123
BspDI ATCGAT 1 cut(s) 147
BspHI TCATGA 1 cut(s) 39
BspLI GGNNCC 1 cut(s) 127
BssMI GATC 2 cut(s) 148, 187
BstKTI GATC 2 cut(s) 151, 190
BstMBI GATC 2 cut(s) 148, 187
BstV1I GCAGC 1 cut(s) 184
Bsu15I ATCGAT 1 cut(s) 147
BsuTUI ATCGAT 1 cut(s) 147
CciI TCATGA 1 cut(s) 39
ClaI ATCGAT 1 cut(s) 147
CseI GACGC 1 cut(s) 231
Csp6I GTAC 1 cut(s) 29
CviAII CATG 2 cut(s) 40, 70
CviJI RGCY 3 cut(s) 128, 175, 245
CviKI_1 RGCY 3 cut(s) 128, 175, 245
CviQI GTAC 1 cut(s) 29
DpnI GATC 2 cut(s) 150, 189
DpnII GATC 2 cut(s) 148, 187
EciI GGCGGA 1 cut(s) 138
FaeI CATG 2 cut(s) 43, 73
FaiI YATR 6 cut(s) 41, 71, 240, 242, 271, 277
FatI CATG 2 cut(s) 39, 69
Fnu4HI GCNGC 1 cut(s) 173
Fsp4HI GCNGC 1 cut(s) 173
GluI GCNGC 1 cut(s) 173
HgaI GACGC 1 cut(s) 231
Hin1II CATG 2 cut(s) 43, 73
HincII GTYRAC 1 cut(s) 258
HindII GTYRAC 1 cut(s) 258
HinfI GANTC 1 cut(s) 139
HpaI GTTAAC 1 cut(s) 258
Hpy166II GTNNAC 2 cut(s) 31, 258
Hpy188I TCNGA 1 cut(s) 235
Hpy188III TCNNGA 1 cut(s) 40
Hpy8I GTNNAC 2 cut(s) 31, 258
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 4 cut(s) 113, 189, 212, 246
Hsp92II CATG 2 cut(s) 43, 73
KspAI GTTAAC 1 cut(s) 258
Kzo9I GATC 2 cut(s) 148, 187
LmnI GCTCC 1 cut(s) 125
Lsp1109I GCAGC 1 cut(s) 184
MalI GATC 2 cut(s) 150, 189
MboI GATC 2 cut(s) 148, 187
MluCI AATT 2 cut(s) 152, 204
MmeI TCCRAC 1 cut(s) 112
MnlI CCTC 1 cut(s) 41
MseI TTAA 2 cut(s) 156, 257
NdeII GATC 2 cut(s) 148, 187
NlaIII CATG 2 cut(s) 43, 73
NlaIV GGNNCC 1 cut(s) 127
PagI TCATGA 1 cut(s) 39
PfeI GAWTC 1 cut(s) 139
PkrI GCNGC 1 cut(s) 174
PspN4I GGNNCC 1 cut(s) 127
RsaI GTAC 1 cut(s) 30
RsaNI GTAC 1 cut(s) 29
SaqAI TTAA 2 cut(s) 156, 257
SatI GCNGC 1 cut(s) 173
Sau3AI GATC 2 cut(s) 148, 187
SetI ASST 3 cut(s) 111, 177, 257
SgeI CNNG 5 cut(s) 52, 82, 181, 226, 261
Sse9I AATT 2 cut(s) 152, 204
SsiI CCGC 1 cut(s) 123
TaqI TCGA 1 cut(s) 147
TasI AATT 2 cut(s) 152, 204
TfiI GAWTC 1 cut(s) 139
Tru1I TTAA 2 cut(s) 156, 257
Tru9I TTAA 2 cut(s) 156, 257
TseI GCWGC 1 cut(s) 172
TspDTI ATGAA 2 cut(s) 254, 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.