pycom12g02660

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0024417
Physical Location & Seq
Reverse (-)
2394099 .. 2396139
2041 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1131 bp
ATGGCTGAGCAACCAAAAGGCCGGGCAGTTGGTGCCATGGAGAACAGCTGGTGCCGGGCGGTCCCCGGCGGCACGGGCATAACGGTGCTAGGTTTTGACATATCCAAAGCCCCGGATATGGTCAAATACCAAACAGCTCTTCACAAACTCCAGAATGCCCACCCAATCCTCAACTCTAGGCTCCATACCAATACCAAAGCAAACACATTCTCCTTTGTCACATCCCCTACTCCTTTTGTCCAAATCAAAACATTGGACCTCTCATCAACTTTGGAAATCCTTGAAACCCTCTCAAACCCTAGCAACAGTTCAGTCTCTCCCTTCCATCTGATCCTTGAGTATGAGCTCAACCAAAACACTTGGACTAAAACTACTTCGTCGTCATCGTCGTCTCCGCCAAATGATCATGACATGTTCTTTGGAACCATCTACACCCTACCGGACGCCAAGTGGGTGGCAGTGATGAGGTTCCACGTGGCAGCATGCGACCACACTACAGCTGAGACTATGCTGAGAGAGTTGCTTGGGTTCTTGACGAGTGCTGATGATGGTGATGATGGTGGAGAGGGGAAAGGGATAGTGAAGGAAACTGGGAATAAAGGGGAGGCCAATTTTGGAATTGAGGATCTCGTTCCTGCTAGGGTTGCTAAGAAACCCTTTTGGAGTCGTGGGGGTGACATGATTAGTTACTCTCTGAATACCGTCAGGCTGTCAAGTTTGAAATTCAAAGACTACAAATCAGTTAGATCGTCCCAGGTGGTGAGGTTTCAGATGAATCAAGATGAAACGAAGAGGATTCTCACTGGATGCAAGGCCAGAGAGATTAAACTATGTGGAGCATTGGTGGCTGCTGGATTGATTGCAGCAGATAAATGCTCGAAACCTAATCAAAAGAAGAAGTATGGAGTGCTGACACTCATTGATTGCCGCTCCGATCGATCCAGCTCTTTCAATTCACCACTTTGGGAATTACCACTCTATCATCCCCCTCGTCCACACCATAAAAGGAGGAGAAAATCTATGGGAGCTGGCCAAGAAAACCTACACGGCCTTTGCAAACTCCAAAAAGAACGACAAACACTTCACGGATTTGGCTACCCTGAGCTTCGTGATGTGCAAGGCCATCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

377

Amino Acids

41.71

Weight (kDa)

9.32

Isoelectric Point (pI)

39.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024417)

Species Orthologous Gene IDs
malus_domestica MD12G1027900.v1.1
prunus_persica Prupe.7G103800_v2.0.a1
pyrus_communis pycom12g02660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 32, 51
AccBSI CCGCTC 1 cut(s) 930
AciI CCGC 4 cut(s) 59, 69, 395, 928
AclWI GGATC 3 cut(s) 325, 633, 933
AcoI YGGCCR 1 cut(s) 1030
AcsI RAATTY 1 cut(s) 722
AcvI CACGTG 1 cut(s) 475
AcyI GRCGYC 1 cut(s) 444
AfiI CCNNNNNNNGG 1 cut(s) 118
AflIII ACRYGT 1 cut(s) 411
AgsI TTSAA 4 cut(s) 284, 721, 727, 952
AjnI CCWGG 1 cut(s) 753
AjuI GAANNNNNNNTTGG 2 cut(s) 123, 155
AluBI AGCT 7 cut(s) 48, 137, 346, 500, 945, 1028, 1105
AluI AGCT 7 cut(s) 48, 137, 346, 500, 945, 1028, 1105
Alw21I GWGCWC 1 cut(s) 348
Alw26I GTCTC 3 cut(s) 319, 396, 497
AlwI GGATC 3 cut(s) 325, 633, 933
AoxI GGCC 6 cut(s) 19, 606, 813, 1030, 1048, 1120
ApeKI GCWGC 3 cut(s) 479, 848, 863
ApoI RAATTY 1 cut(s) 722
ArsI GACNNNNNNTTYG 2 cut(s) 401, 433
AspS9I GGNCC 2 cut(s) 61, 256
AsuC2I CCSGG 4 cut(s) 23, 56, 66, 113
AsuHPI GGTGA 4 cut(s) 563, 686, 772, 948
AvaII GGWCC 2 cut(s) 61, 256
BalI TGGCCA 1 cut(s) 1032
BanI GGYRCC 2 cut(s) 32, 51
BanII GRGCYC 1 cut(s) 348
BbrPI CACGTG 1 cut(s) 475
Bbv12I GWGCWC 1 cut(s) 348
BbvI GCAGC 3 cut(s) 491, 835, 875
BccI CCATC 4 cut(s) 333, 434, 542, 551
BceAI ACGGC 1 cut(s) 1063
BcgI CGANNNNNNTGC 1 cut(s) 1106
BciT130I CCWGG 1 cut(s) 755
BclI TGATCA 1 cut(s) 403
BcnI CCSGG 4 cut(s) 23, 56, 66, 113
BcoDI GTCTC 3 cut(s) 319, 396, 497
BfaI CTAG 4 cut(s) 89, 177, 300, 639
BfmI CTRYAG 1 cut(s) 495
BisI GCNGC 5 cut(s) 70, 480, 849, 864, 928
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 5 cut(s) 71, 481, 850, 865, 929
Bme1390I CCNGG 5 cut(s) 23, 56, 66, 113, 755
Bme18I GGWCC 2 cut(s) 61, 256
BmgT120I GGNCC 2 cut(s) 61, 256
BmiI GGNNCC 6 cut(s) 34, 53, 63, 182, 424, 470
BmrFI CCNGG 5 cut(s) 23, 56, 66, 113, 755
BmrI ACTGGG 1 cut(s) 600
BmsI GCATC 1 cut(s) 797
BmuI ACTGGG 1 cut(s) 600
BpmI CTGGAG 1 cut(s) 134
Bpu10I CCTNAGC 1 cut(s) 1101
Bpu1102I GCTNAGC 1 cut(s) 6
BpuEI CTTGAG 1 cut(s) 356
BpuMI CCSGG 4 cut(s) 23, 56, 66, 113
Bsa29I ATCGAT 2 cut(s) 937, 1126
BsaAI YACGTR 1 cut(s) 475
BsaHI GRCGYC 1 cut(s) 444
BsaJI CCNNGG 4 cut(s) 36, 64, 111, 753
BsaWI WCCGGW 1 cut(s) 439
BsaXI ACNNNNNCTCC 4 cut(s) 194, 224, 828, 858
Bsc4I CCNNNNNNNGG 1 cut(s) 118
Bse1I ACTGG 2 cut(s) 595, 808
BseBI CCWGG 1 cut(s) 755
BseCI ATCGAT 2 cut(s) 937, 1126
BseDI CCNNGG 4 cut(s) 36, 64, 111, 753
BseGI GGATG 3 cut(s) 221, 812, 982
BseLI CCNNNNNNNGG 1 cut(s) 118
BseMII CTCAG 3 cut(s) 492, 503, 1092
BseNI ACTGG 2 cut(s) 595, 808
BseRI GAGGAG 1 cut(s) 1024
BseXI GCAGC 3 cut(s) 491, 835, 875
Bsh1285I CGRYCG 1 cut(s) 937
BshFI GGCC 6 cut(s) 21, 608, 815, 1032, 1050, 1122
BshNI GGYRCC 2 cut(s) 32, 51
BshVI ATCGAT 2 cut(s) 937, 1126
BsiEI CGRYCG 1 cut(s) 937
BsiHKAI GWGCWC 1 cut(s) 348
BsiSI CCGG 5 cut(s) 22, 55, 66, 113, 440
BslFI GGGAC 2 cut(s) 47, 736
BslI CCNNNNNNNGG 1 cut(s) 118
BsmAI GTCTC 3 cut(s) 319, 396, 497
BsmBI CGTCTC 1 cut(s) 396
BsmFI GGGAC 2 cut(s) 47, 736
BsmI GAATGC 1 cut(s) 160
BsnI GGCC 6 cut(s) 21, 608, 815, 1032, 1050, 1122
Bsp1286I GDGCHC 1 cut(s) 348
Bsp143I GATC 6 cut(s) 330, 403, 625, 746, 934, 938
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 1 cut(s) 36
BspACI CCGC 4 cut(s) 59, 69, 395, 928
BspANI GGCC 6 cut(s) 21, 608, 815, 1032, 1050, 1122
BspCNI CTCAG 3 cut(s) 493, 504, 1093
BspDI ATCGAT 2 cut(s) 937, 1126
BspHI TCATGA 1 cut(s) 406
BspLI GGNNCC 6 cut(s) 34, 53, 63, 182, 424, 470
BspPI GGATC 3 cut(s) 325, 633, 933
BspQI GCTCTTC 1 cut(s) 144
BspT107I GGYRCC 2 cut(s) 32, 51
BsrBI CCGCTC 1 cut(s) 930
BsrI ACTGG 2 cut(s) 595, 808
BssECI CCNNGG 4 cut(s) 36, 64, 111, 753
BssMI GATC 6 cut(s) 330, 403, 625, 746, 934, 938
BssNI GRCGYC 1 cut(s) 444
BssT1I CCWWGG 1 cut(s) 36
Bst2UI CCWGG 1 cut(s) 755
Bst4CI ACNGT 3 cut(s) 85, 308, 703
Bst6I CTCTTC 2 cut(s) 144, 785
BstACI GRCGYC 1 cut(s) 444
BstAPI GCANNNNNTGC 1 cut(s) 32
BstBAI YACGTR 1 cut(s) 475
BstC8I GCNNGC 2 cut(s) 484, 1030
BstDEI CTNAG 5 cut(s) 6, 501, 512, 648, 1101
BstDSI CCRYGG 1 cut(s) 36
BstF5I GGATG 3 cut(s) 221, 812, 982
BstKTI GATC 6 cut(s) 333, 406, 628, 749, 937, 941
BstMAI GTCTC 3 cut(s) 319, 396, 497
BstMBI GATC 6 cut(s) 330, 403, 625, 746, 934, 938
BstMCI CGRYCG 1 cut(s) 937
BstMWI GCNNNNNNNGC 4 cut(s) 32, 75, 644, 845
BstNI CCWGG 1 cut(s) 755
BstNSI RCATGY 2 cut(s) 415, 486
BstSCI CCNGG 5 cut(s) 21, 54, 64, 111, 753
BstSFI CTRYAG 1 cut(s) 495
BstV1I GCAGC 3 cut(s) 491, 835, 875
BstX2I RGATCY 1 cut(s) 625
BstXI CCANNNNNNTGG 1 cut(s) 454
BstYI RGATCY 1 cut(s) 625
Bsu15I ATCGAT 2 cut(s) 937, 1126
BsuRI GGCC 6 cut(s) 21, 608, 815, 1032, 1050, 1122
BsuTUI ATCGAT 2 cut(s) 937, 1126
BtgI CCRYGG 1 cut(s) 36
BtsCI GGATG 3 cut(s) 221, 812, 982
BtsI GCAGTG 1 cut(s) 465
BtsIMutI CAGTG 2 cut(s) 465, 801
Cac8I GCNNGC 2 cut(s) 484, 1030
CciI TCATGA 1 cut(s) 406
Cfr13I GGNCC 2 cut(s) 61, 256
ClaI ATCGAT 2 cut(s) 937, 1126
CseI GACGC 1 cut(s) 452
CviAII CATG 5 cut(s) 37, 407, 412, 483, 679
DdeI CTNAG 5 cut(s) 6, 501, 512, 648, 1101
DpnI GATC 6 cut(s) 332, 405, 627, 748, 936, 940
DpnII GATC 6 cut(s) 330, 403, 625, 746, 934, 938
EaeI YGGCCR 1 cut(s) 1030
Eam1104I CTCTTC 2 cut(s) 144, 785
EarI CTCTTC 2 cut(s) 144, 785
EciI GGCGGA 1 cut(s) 384
Ecl136II GAGCTC 1 cut(s) 346
Eco130I CCWWGG 1 cut(s) 36
Eco24I GRGCYC 1 cut(s) 348
Eco47I GGWCC 2 cut(s) 61, 256
Eco53kI GAGCTC 1 cut(s) 346
Eco72I CACGTG 1 cut(s) 475
EcoICRI GAGCTC 1 cut(s) 346
EcoRII CCWGG 1 cut(s) 753
EcoT14I CCWWGG 1 cut(s) 36
EcoT38I GRGCYC 1 cut(s) 348
ErhI CCWWGG 1 cut(s) 36
Esp3I CGTCTC 1 cut(s) 396
FaeI CATG 5 cut(s) 40, 410, 415, 486, 682
FalI AAGNNNNNCTT 2 cut(s) 641, 673
FaqI GGGAC 2 cut(s) 47, 736
FatI CATG 5 cut(s) 36, 406, 411, 482, 678
FbaI TGATCA 1 cut(s) 403
Fnu4HI GCNGC 5 cut(s) 70, 480, 849, 864, 928
FokI GGATG 3 cut(s) 208, 819, 969
FriOI GRGCYC 1 cut(s) 348
Fsp4HI GCNGC 5 cut(s) 70, 480, 849, 864, 928
FspBI CTAG 4 cut(s) 89, 177, 300, 639
GluI GCNGC 5 cut(s) 70, 480, 849, 864, 928
GsuI CTGGAG 1 cut(s) 134
HaeIII GGCC 6 cut(s) 21, 608, 815, 1032, 1050, 1122
HapII CCGG 5 cut(s) 22, 55, 66, 113, 440
HgaI GACGC 1 cut(s) 452
Hin1I GRCGYC 1 cut(s) 444
Hin1II CATG 5 cut(s) 40, 410, 415, 486, 682
HinfI GANTC 3 cut(s) 664, 775, 796
HpaII CCGG 5 cut(s) 22, 55, 66, 113, 440
HphI GGTGA 4 cut(s) 563, 686, 772, 948
Hpy166II GTNNAC 1 cut(s) 995
Hpy188I TCNGA 4 cut(s) 330, 696, 771, 934
Hpy188III TCNNGA 5 cut(s) 151, 407, 532, 779, 1109
Hpy8I GTNNAC 1 cut(s) 995
Hpy99I CGWCG 2 cut(s) 382, 391
HpyAV CCTTC 2 cut(s) 331, 577
HpyCH4III ACNGT 3 cut(s) 85, 308, 703
HpyCH4IV ACGT 1 cut(s) 474
HpyCH4V TGCA 4 cut(s) 810, 863, 1056, 1117
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 75, 644, 845
HpyF3I CTNAG 5 cut(s) 6, 501, 512, 648, 1101
HpySE526I ACGT 1 cut(s) 474
Hsp92I GRCGYC 1 cut(s) 444
Hsp92II CATG 5 cut(s) 40, 410, 415, 486, 682
Ksp22I TGATCA 1 cut(s) 403
Kzo9I GATC 6 cut(s) 330, 403, 625, 746, 934, 938
LguI GCTCTTC 1 cut(s) 144
LmnI GCTCC 4 cut(s) 186, 836, 935, 1025
Lsp1109I GCAGC 3 cut(s) 491, 835, 875
LweI GCATC 1 cut(s) 797
MaeI CTAG 4 cut(s) 89, 177, 300, 639
MaeII ACGT 1 cut(s) 474
MaeIII GTNAC 3 cut(s) 217, 674, 686
MalI GATC 6 cut(s) 332, 405, 627, 748, 936, 940
MbiI CCGCTC 1 cut(s) 930
MboI GATC 6 cut(s) 330, 403, 625, 746, 934, 938
MboII GAAGA 3 cut(s) 131, 802, 907
MflI RGATCY 1 cut(s) 625
MhlI GDGCHC 1 cut(s) 348
MlsI TGGCCA 1 cut(s) 1032
MluCI AATT 5 cut(s) 610, 618, 722, 952, 968
MluNI TGGCCA 1 cut(s) 1032
MlyI GAGTC 1 cut(s) 673
Mox20I TGGCCA 1 cut(s) 1032
MscI TGGCCA 1 cut(s) 1032
MseI TTAA 1 cut(s) 825
MslI CAYNNNNRTG 1 cut(s) 83
Msp20I TGGCCA 1 cut(s) 1032
MspA1I CMGCKG 2 cut(s) 48, 500
MspI CCGG 5 cut(s) 22, 55, 66, 113, 440
MspR9I CCNGG 5 cut(s) 23, 56, 66, 113, 755
Mva1269I GAATGC 1 cut(s) 160
MvaI CCWGG 1 cut(s) 755
MwoI GCNNNNNNNGC 4 cut(s) 32, 75, 644, 845
NciI CCSGG 4 cut(s) 23, 56, 66, 113
NcoI CCATGG 1 cut(s) 36
NdeII GATC 6 cut(s) 330, 403, 625, 746, 934, 938
NlaIII CATG 5 cut(s) 40, 410, 415, 486, 682
NlaIV GGNNCC 6 cut(s) 34, 53, 63, 182, 424, 470
NmuCI GTSAC 2 cut(s) 217, 674
NspI RCATGY 2 cut(s) 415, 486
PaeI GCATGC 1 cut(s) 486
PagI TCATGA 1 cut(s) 406
PciI ACATGT 1 cut(s) 411
PciSI GCTCTTC 1 cut(s) 144
PcsI WCGNNNNNNNCGW 2 cut(s) 383, 386
PctI GAATGC 1 cut(s) 160
PfeI GAWTC 2 cut(s) 775, 796
PkrI GCNGC 5 cut(s) 71, 481, 850, 865, 929
Ple19I CGATCG 1 cut(s) 937
PleI GAGTC 1 cut(s) 672
PmaCI CACGTG 1 cut(s) 475
PmlI CACGTG 1 cut(s) 475
PpsI GAGTC 1 cut(s) 672
Ppu21I YACGTR 1 cut(s) 475
PscI ACATGT 1 cut(s) 411
Psp124BI GAGCTC 1 cut(s) 348
Psp6I CCWGG 1 cut(s) 753
PspCI CACGTG 1 cut(s) 475
PspGI CCWGG 1 cut(s) 753
PspN4I GGNNCC 6 cut(s) 34, 53, 63, 182, 424, 470
PspPI GGNCC 2 cut(s) 61, 256
PsuI RGATCY 1 cut(s) 625
PvuI CGATCG 1 cut(s) 937
PvuII CAGCTG 2 cut(s) 48, 500
RseI CAYNNNNRTG 1 cut(s) 83
SacI GAGCTC 1 cut(s) 348
SapI GCTCTTC 1 cut(s) 144
SaqAI TTAA 1 cut(s) 825
SatI GCNGC 5 cut(s) 70, 480, 849, 864, 928
Sau3AI GATC 6 cut(s) 330, 403, 625, 746, 934, 938
Sau96I GGNCC 2 cut(s) 61, 256
SchI GAGTC 1 cut(s) 673
ScrFI CCNGG 5 cut(s) 23, 56, 66, 113, 755
SduI GDGCHC 1 cut(s) 348
SfaNI GCATC 1 cut(s) 797
SfcI CTRYAG 1 cut(s) 495
SinI GGWCC 2 cut(s) 61, 256
SmiMI CAYNNNNRTG 1 cut(s) 83
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
SphI GCATGC 1 cut(s) 486
Sse9I AATT 5 cut(s) 610, 618, 722, 952, 968
SsiI CCGC 4 cut(s) 59, 69, 395, 928
SspMI CTAG 4 cut(s) 89, 177, 300, 639
SstI GAGCTC 1 cut(s) 348
StyD4I CCNGG 5 cut(s) 21, 54, 64, 111, 753
StyI CCWWGG 1 cut(s) 36
TaaI ACNGT 3 cut(s) 85, 308, 703
TaiI ACGT 1 cut(s) 477
TaqI TCGA 3 cut(s) 878, 937, 1126
TasI AATT 5 cut(s) 610, 618, 722, 952, 968
TauI GCSGC 2 cut(s) 72, 930
TfiI GAWTC 2 cut(s) 775, 796
Tru1I TTAA 1 cut(s) 825
Tru9I TTAA 1 cut(s) 825
TscAI CASTG 2 cut(s) 465, 808
TseFI GTSAC 2 cut(s) 217, 674
TseI GCWGC 3 cut(s) 479, 848, 863
Tsp45I GTSAC 2 cut(s) 217, 674
TspDTI ATGAA 2 cut(s) 788, 798
TspGWI ACGGA 1 cut(s) 1101
TspRI CASTG 2 cut(s) 465, 808
VpaK11BI GGWCC 2 cut(s) 61, 256
XapI RAATTY 1 cut(s) 722
XceI RCATGY 2 cut(s) 415, 486
XspI CTAG 4 cut(s) 89, 177, 300, 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.