pycom12g03610

Trafficking protein particle complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0029421
Physical Location & Seq
Forward (+)
3517998 .. 3519555
1558 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 477 bp
ATGGTTGATCCGGCGAATACGCCACTTGGCAGAATGTTATTGGAGGAGATCACTCCCGTCGTCATGGTCCTCCGTACGCCCCTCGTTGAAGAAGCTTGCCTCAAGAACGGCCTCACCTTCGTTCAAATGCTGAAGCCCTTCTGCGTCTTCAACAACATCGACGTGCCGGTGCGGACTGCGATCGATCAACCCTACAGGCTTCAGAAGTTCGGGTTGCGGTTGTTTTATGAGTCGGATATTCGGCAACCAAACTTAGAGGTAGCAAAAGAGAGGTTGAAACAAGTCATAACCCAGGCTGCGGAGAAAGATCGATTTCTGAATTGTGCTCGGACCTGCCTCAAATTGATAATGCATTATCCATATCGGAATCTGAAGTTCAACCATCATGGTTTCAGTTTTTCAATAAAGAGCTTGTGCATACGGTGTCTTTTTCAGACCACGAAGCTTTTGATCATCCTGTGGCATGTCTCATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRAPPC-Trs85 TRAPPC9-Trs120

Protein Analysis

159

Amino Acids

18.44

Weight (kDa)

9.38

Isoelectric Point (pI)

43.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAPPC-Trs85 PF12739 16 - 123 3e-09 ER-Golgi trafficking TRAPP I complex 85 kDa subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029421)

Species Orthologous Gene IDs
pyrus_communis pycom12g03610
rosa_samantha Rh3DG230400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 341
AciI CCGC 3 cut(s) 172, 217, 299
AclWI GGATC 1 cut(s) 2
AcuI CTGAAG 3 cut(s) 152, 185, 392
AfaI GTAC 1 cut(s) 76
AfiI CCNNNNNNNGG 1 cut(s) 298
AgsI TTSAA 6 cut(s) 89, 125, 151, 277, 379, 402
AjiI CACGTC 1 cut(s) 163
AjnI CCWGG 1 cut(s) 291
AluBI AGCT 3 cut(s) 95, 411, 445
AluI AGCT 3 cut(s) 95, 411, 445
Alw21I GWGCWC 1 cut(s) 328
Alw26I GTCTC 1 cut(s) 472
AlwI GGATC 1 cut(s) 2
AoxI GGCC 1 cut(s) 109
ApeKI GCWGC 1 cut(s) 296
Asp700I GAANNNNTTC 1 cut(s) 137
AspS9I GGNCC 2 cut(s) 67, 330
AsuHPI GGTGA 1 cut(s) 106
AvaII GGWCC 2 cut(s) 67, 330
BbsI GAAGAC 1 cut(s) 139
Bbv12I GWGCWC 1 cut(s) 328
BbvI GCAGC 1 cut(s) 283
BccI CCATC 1 cut(s) 390
BceAI ACGGC 1 cut(s) 124
BciT130I CCWGG 1 cut(s) 293
BclI TGATCA 1 cut(s) 450
BcoDI GTCTC 1 cut(s) 472
BfmI CTRYAG 1 cut(s) 193
BfuAI ACCTGC 1 cut(s) 341
BisI GCNGC 1 cut(s) 297
BlsI GCNGC 1 cut(s) 298
Bme1390I CCNGG 1 cut(s) 293
Bme18I GGWCC 2 cut(s) 67, 330
BmgBI CACGTC 1 cut(s) 163
BmgT120I GGNCC 2 cut(s) 67, 330
BmrFI CCNGG 1 cut(s) 293
BpiI GAAGAC 1 cut(s) 139
BpuEI CTTGAG 1 cut(s) 86
Bsa29I ATCGAT 2 cut(s) 183, 310
BsaJI CCNNGG 1 cut(s) 291
Bsc4I CCNNNNNNNGG 1 cut(s) 298
Bse118I RCCGGY 1 cut(s) 166
BseBI CCWGG 1 cut(s) 293
BseCI ATCGAT 2 cut(s) 183, 310
BseDI CCNNGG 1 cut(s) 291
BseGI GGATG 1 cut(s) 453
BseLI CCNNNNNNNGG 1 cut(s) 298
BseRI GAGGAG 1 cut(s) 59
BseXI GCAGC 1 cut(s) 283
Bsh1285I CGRYCG 1 cut(s) 183
BshFI GGCC 1 cut(s) 111
BshVI ATCGAT 2 cut(s) 183, 310
BsiEI CGRYCG 1 cut(s) 183
BsiHKAI GWGCWC 1 cut(s) 328
BsiSI CCGG 2 cut(s) 11, 167
BsiWI CGTACG 1 cut(s) 74
BslI CCNNNNNNNGG 1 cut(s) 298
BsmAI GTCTC 1 cut(s) 472
BsnI GGCC 1 cut(s) 111
Bsp1286I GDGCHC 1 cut(s) 328
Bsp143I GATC 6 cut(s) 7, 48, 180, 184, 307, 450
BspACI CCGC 3 cut(s) 172, 217, 299
BspANI GGCC 1 cut(s) 111
BspDI ATCGAT 2 cut(s) 183, 310
BspMI ACCTGC 1 cut(s) 341
BspPI GGATC 1 cut(s) 2
BsrFI RCCGGY 1 cut(s) 166
BssAI RCCGGY 1 cut(s) 166
BssECI CCNNGG 1 cut(s) 291
BssMI GATC 6 cut(s) 7, 48, 180, 184, 307, 450
Bst2UI CCWGG 1 cut(s) 293
Bst4CI ACNGT 1 cut(s) 423
BstC8I GCNNGC 1 cut(s) 97
BstDEI CTNAG 1 cut(s) 253
BstF5I GGATG 1 cut(s) 453
BstKTI GATC 6 cut(s) 10, 51, 183, 187, 310, 453
BstMAI GTCTC 1 cut(s) 472
BstMBI GATC 6 cut(s) 7, 48, 180, 184, 307, 450
BstMCI CGRYCG 1 cut(s) 183
BstNI CCWGG 1 cut(s) 293
BstNSI RCATGY 1 cut(s) 467
BstSCI CCNGG 1 cut(s) 291
BstSFI CTRYAG 1 cut(s) 193
BstV1I GCAGC 1 cut(s) 283
BstV2I GAAGAC 1 cut(s) 139
Bsu15I ATCGAT 2 cut(s) 183, 310
BsuRI GGCC 1 cut(s) 111
BsuTUI ATCGAT 2 cut(s) 183, 310
BtrI CACGTC 1 cut(s) 163
BtsCI GGATG 1 cut(s) 453
BveI ACCTGC 1 cut(s) 341
Cac8I GCNNGC 1 cut(s) 97
Cfr10I RCCGGY 1 cut(s) 166
Cfr13I GGNCC 2 cut(s) 67, 330
ClaI ATCGAT 2 cut(s) 183, 310
CseI GACGC 1 cut(s) 133
Csp6I GTAC 1 cut(s) 75
CviAII CATG 3 cut(s) 64, 386, 464
CviJI RGCY 7 cut(s) 95, 111, 136, 199, 296, 411, 445
CviKI_1 RGCY 7 cut(s) 95, 111, 136, 199, 296, 411, 445
CviQI GTAC 1 cut(s) 75
DdeI CTNAG 1 cut(s) 253
DpnI GATC 6 cut(s) 9, 50, 182, 186, 309, 452
DpnII GATC 6 cut(s) 7, 48, 180, 184, 307, 450
Eco47I GGWCC 2 cut(s) 67, 330
Eco57I CTGAAG 3 cut(s) 152, 185, 392
EcoRII CCWGG 1 cut(s) 291
EcoT22I ATGCAT 1 cut(s) 354
FaeI CATG 3 cut(s) 67, 389, 467
FaiI YATR 7 cut(s) 65, 228, 287, 361, 387, 419, 465
FatI CATG 3 cut(s) 63, 385, 463
FbaI TGATCA 1 cut(s) 450
Fnu4HI GCNGC 1 cut(s) 297
FokI GGATG 1 cut(s) 440
Fsp4HI GCNGC 1 cut(s) 297
GluI GCNGC 1 cut(s) 297
HaeIII GGCC 1 cut(s) 111
HapII CCGG 2 cut(s) 11, 167
HgaI GACGC 1 cut(s) 133
Hin1II CATG 3 cut(s) 67, 389, 467
HindIII AAGCTT 2 cut(s) 93, 443
HinfI GANTC 2 cut(s) 230, 367
HpaII CCGG 2 cut(s) 11, 167
HphI GGTGA 1 cut(s) 106
Hpy188I TCNGA 7 cut(s) 204, 235, 318, 330, 366, 372, 435
Hpy188III TCNNGA 1 cut(s) 103
Hpy99I CGWCG 2 cut(s) 62, 164
HpyAV CCTTC 2 cut(s) 127, 148
HpyCH4III ACNGT 1 cut(s) 423
HpyCH4IV ACGT 1 cut(s) 162
HpyCH4V TGCA 2 cut(s) 352, 417
HpyF3I CTNAG 1 cut(s) 253
HpySE526I ACGT 1 cut(s) 162
Hsp92II CATG 3 cut(s) 67, 389, 467
Ksp22I TGATCA 1 cut(s) 450
Kzo9I GATC 6 cut(s) 7, 48, 180, 184, 307, 450
LpnPI CCDG 7 cut(s) 24, 180, 181, 278, 305, 346, 470
Lsp1109I GCAGC 1 cut(s) 283
MaeII ACGT 1 cut(s) 162
MalI GATC 6 cut(s) 9, 50, 182, 186, 309, 452
MboI GATC 6 cut(s) 7, 48, 180, 184, 307, 450
MboII GAAGA 2 cut(s) 101, 139
MhlI GDGCHC 1 cut(s) 328
MluCI AATT 2 cut(s) 319, 341
MlyI GAGTC 1 cut(s) 239
MmeI TCCRAC 1 cut(s) 213
MnlI CCTC 8 cut(s) 37, 80, 92, 110, 122, 250, 264, 347
Mph1103I ATGCAT 1 cut(s) 354
MroXI GAANNNNTTC 1 cut(s) 137
MslI CAYNNNNRTG 1 cut(s) 161
MspI CCGG 2 cut(s) 11, 167
MspR9I CCNGG 1 cut(s) 293
MvaI CCWGG 1 cut(s) 293
NdeII GATC 6 cut(s) 7, 48, 180, 184, 307, 450
NlaIII CATG 3 cut(s) 67, 389, 467
NsiI ATGCAT 1 cut(s) 354
NspI RCATGY 1 cut(s) 467
PdmI GAANNNNTTC 1 cut(s) 137
PfeI GAWTC 1 cut(s) 367
Pfl23II CGTACG 1 cut(s) 74
PkrI GCNGC 1 cut(s) 298
Ple19I CGATCG 1 cut(s) 183
PleI GAGTC 1 cut(s) 238
PpsI GAGTC 1 cut(s) 238
Psp6I CCWGG 1 cut(s) 291
PspGI CCWGG 1 cut(s) 291
PspLI CGTACG 1 cut(s) 74
PspPI GGNCC 2 cut(s) 67, 330
PvuI CGATCG 1 cut(s) 183
RsaI GTAC 1 cut(s) 76
RsaNI GTAC 1 cut(s) 75
RseI CAYNNNNRTG 1 cut(s) 161
SatI GCNGC 1 cut(s) 297
Sau3AI GATC 6 cut(s) 7, 48, 180, 184, 307, 450
Sau96I GGNCC 2 cut(s) 67, 330
SchI GAGTC 1 cut(s) 239
ScrFI CCNGG 1 cut(s) 293
SduI GDGCHC 1 cut(s) 328
SetI ASST 8 cut(s) 97, 119, 165, 261, 275, 335, 413, 447
SfcI CTRYAG 1 cut(s) 193
SinI GGWCC 2 cut(s) 67, 330
SmiMI CAYNNNNRTG 1 cut(s) 161
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
Sse9I AATT 2 cut(s) 319, 341
SsiI CCGC 3 cut(s) 172, 217, 299
StyD4I CCNGG 1 cut(s) 291
TaaI ACNGT 1 cut(s) 423
TaiI ACGT 1 cut(s) 165
TaqI TCGA 3 cut(s) 159, 183, 310
TasI AATT 2 cut(s) 319, 341
TfiI GAWTC 1 cut(s) 367
TseI GCWGC 1 cut(s) 296
TspGWI ACGGA 1 cut(s) 62
VpaK11BI GGWCC 2 cut(s) 67, 330
XceI RCATGY 1 cut(s) 467
XmnI GAANNNNTTC 1 cut(s) 137
Zsp2I ATGCAT 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.