pycom12g03900

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0028766
Physical Location & Seq
Forward (+)
3778025 .. 3779411
1387 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 501 bp
ATGGTTTTCAACTTTCATTGCTTTCTTCTATTCTCCATCTTCTATTTATCCATTATCCCCATTTTTTCCCTCGACATTGTTACAAGATGTGATTCAAATGGTTACACTGCCAACACCTTTTTGGATACAGTGACTGCTGCAATTGTACAGAGGAAGGCACCGGAATGGCCAAGAGCAGTAGCAACGAAGAAGATGAATGTGATTAAGGGTGCACTGCAGCAAATGCGGCATAGAAACAGGACGAGATGTCCGAGAGGAACGATTCCTATACGGCGGAGCACAGTGGATGATGTGCTGAGGACCAAGTCTATGGTTGACTTTGGCAAGAAACAGCACCGTACCCTAAACATTACTGCTGATCTTGCTTCCATACGCATCGATGCCCCTGATGTAGTCAGCGGGAATGGCCATGAGCATGCAATCGCGTACACTAGAACATCCAAGGAGTGTATGGCGCTCGGGCGACAATCAATGTGTGGTCCCCTTCGATCGAACACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

167

Amino Acids

18.7

Weight (kDa)

10.11

Isoelectric Point (pI)

32.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 49 - 143 9.6e-18 Neprosin activation peptide
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028766)

Species Orthologous Gene IDs
malus_domestica MD17G1124600.v1.1
pyrus_communis pycom12g03900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 157
AccII CGCG 1 cut(s) 425
AciI CCGC 3 cut(s) 226, 274, 399
AcoI YGGCCR 2 cut(s) 167, 406
AfaI GTAC 3 cut(s) 147, 340, 428
AflIII ACRYGT 1 cut(s) 495
AgsI TTSAA 2 cut(s) 10, 96
AhdI GACNNNNNGTC 1 cut(s) 246
Alw21I GWGCWC 2 cut(s) 214, 281
Alw44I GTGCAC 1 cut(s) 210
AlwNI CAGNNNCTG 1 cut(s) 134
Ama87I CYCGRG 1 cut(s) 458
AoxI GGCC 2 cut(s) 167, 406
ApaLI GTGCAC 1 cut(s) 210
ApeKI GCWGC 2 cut(s) 137, 217
AspLEI GCGC 1 cut(s) 457
AspS9I GGNCC 2 cut(s) 300, 479
AvaI CYCGRG 1 cut(s) 458
AvaII GGWCC 2 cut(s) 300, 479
BaeGI GKGCMC 1 cut(s) 214
BalI TGGCCA 2 cut(s) 169, 408
BanI GGYRCC 1 cut(s) 157
Bbv12I GWGCWC 2 cut(s) 214, 281
BbvCI CCTCAGC 1 cut(s) 296
BbvI GCAGC 2 cut(s) 124, 229
BccI CCATC 1 cut(s) 44
BceAI ACGGC 1 cut(s) 287
BciVI GTATCC 1 cut(s) 118
BfaI CTAG 1 cut(s) 432
BfmI CTRYAG 1 cut(s) 215
BfoI RGCGCY 1 cut(s) 458
BfuI GTATCC 1 cut(s) 118
BisI GCNGC 3 cut(s) 138, 218, 227
BlsI GCNGC 3 cut(s) 139, 219, 228
Bme18I GGWCC 2 cut(s) 300, 479
BmeRI GACNNNNNGTC 1 cut(s) 246
BmeT110I CYCGRG 1 cut(s) 458
BmgT120I GGNCC 2 cut(s) 300, 479
BmiI GGNNCC 2 cut(s) 159, 481
BmsI GCATC 2 cut(s) 370, 384
Bpu10I CCTNAGC 1 cut(s) 296
Bsa29I ATCGAT 1 cut(s) 378
BsaAI YACGTR 1 cut(s) 498
BsaJI CCNNGG 1 cut(s) 441
BsaWI WCCGGW 1 cut(s) 160
Bse3DI GCAATG 1 cut(s) 16
BseCI ATCGAT 1 cut(s) 378
BseDI CCNNGG 1 cut(s) 441
BseGI GGATG 2 cut(s) 292, 437
BseMI GCAATG 1 cut(s) 16
BseMII CTCAG 1 cut(s) 287
BseSI GKGCMC 1 cut(s) 214
BseXI GCAGC 2 cut(s) 124, 229
Bsh1236I CGCG 1 cut(s) 425
Bsh1285I CGRYCG 1 cut(s) 491
BshFI GGCC 2 cut(s) 169, 408
BshNI GGYRCC 1 cut(s) 157
BshVI ATCGAT 1 cut(s) 378
BsiEI CGRYCG 1 cut(s) 491
BsiHKAI GWGCWC 2 cut(s) 214, 281
BsiHKCI CYCGRG 1 cut(s) 458
BsiSI CCGG 1 cut(s) 161
BslFI GGGAC 1 cut(s) 465
BsmFI GGGAC 1 cut(s) 465
BsnI GGCC 2 cut(s) 169, 408
BsoBI CYCGRG 1 cut(s) 458
Bsp1286I GDGCHC 2 cut(s) 214, 281
Bsp1407I TGTACA 1 cut(s) 145
Bsp143I GATC 2 cut(s) 358, 488
BspACI CCGC 3 cut(s) 226, 274, 399
BspANI GGCC 2 cut(s) 169, 408
BspCNI CTCAG 1 cut(s) 288
BspDI ATCGAT 1 cut(s) 378
BspFNI CGCG 1 cut(s) 425
BspLI GGNNCC 2 cut(s) 159, 481
BspMAI CTGCAG 1 cut(s) 219
BspT107I GGYRCC 1 cut(s) 157
BsrDI GCAATG 1 cut(s) 16
BsrGI TGTACA 1 cut(s) 145
BssECI CCNNGG 1 cut(s) 441
BssMI GATC 2 cut(s) 358, 488
BssT1I CCWWGG 1 cut(s) 441
Bst4CI ACNGT 3 cut(s) 130, 283, 338
BstAPI GCANNNNNTGC 1 cut(s) 223
BstAUI TGTACA 1 cut(s) 145
BstBAI YACGTR 1 cut(s) 498
BstC8I GCNNGC 1 cut(s) 417
BstDEI CTNAG 1 cut(s) 296
BstF5I GGATG 2 cut(s) 292, 437
BstFNI CGCG 1 cut(s) 425
BstH2I RGCGCY 1 cut(s) 458
BstHHI GCGC 1 cut(s) 457
BstKTI GATC 2 cut(s) 361, 491
BstMBI GATC 2 cut(s) 358, 488
BstMCI CGRYCG 1 cut(s) 491
BstMWI GCNNNNNNNGC 4 cut(s) 223, 226, 362, 405
BstNSI RCATGY 1 cut(s) 419
BstSFI CTRYAG 1 cut(s) 215
BstSLI GKGCMC 1 cut(s) 214
BstUI CGCG 1 cut(s) 425
BstV1I GCAGC 2 cut(s) 124, 229
BstXI CCANNNNNNTGG 1 cut(s) 310
Bsu15I ATCGAT 1 cut(s) 378
BsuI GTATCC 1 cut(s) 118
BsuRI GGCC 2 cut(s) 169, 408
BsuTUI ATCGAT 1 cut(s) 378
BtsCI GGATG 2 cut(s) 292, 437
BtsI GCAGTG 2 cut(s) 105, 212
BtsIMutI CAGTG 4 cut(s) 105, 135, 212, 288
Cac8I GCNNGC 1 cut(s) 417
CaiI CAGNNNCTG 1 cut(s) 134
CfoI GCGC 1 cut(s) 457
Cfr13I GGNCC 2 cut(s) 300, 479
ClaI ATCGAT 1 cut(s) 378
Csp6I GTAC 3 cut(s) 146, 339, 427
CviAII CATG 2 cut(s) 410, 416
CviJI RGCY 2 cut(s) 169, 408
CviKI_1 RGCY 2 cut(s) 169, 408
CviQI GTAC 3 cut(s) 146, 339, 427
DdeI CTNAG 1 cut(s) 296
DpnI GATC 2 cut(s) 360, 490
DpnII GATC 2 cut(s) 358, 488
DriI GACNNNNNGTC 1 cut(s) 246
EaeI YGGCCR 2 cut(s) 167, 406
Eam1105I GACNNNNNGTC 1 cut(s) 246
EciI GGCGGA 1 cut(s) 289
Eco130I CCWWGG 1 cut(s) 441
Eco47I GGWCC 2 cut(s) 300, 479
Eco88I CYCGRG 1 cut(s) 458
EcoT14I CCWWGG 1 cut(s) 441
ErhI CCWWGG 1 cut(s) 441
FaeI CATG 2 cut(s) 413, 419
FaiI YATR 7 cut(s) 231, 269, 311, 371, 411, 417, 452
FaqI GGGAC 1 cut(s) 465
FatI CATG 2 cut(s) 409, 415
FauI CCCGC 1 cut(s) 392
Fnu4HI GCNGC 3 cut(s) 138, 218, 227
FokI GGATG 2 cut(s) 299, 424
Fsp4HI GCNGC 3 cut(s) 138, 218, 227
FspBI CTAG 1 cut(s) 432
GlaI GCGC 1 cut(s) 456
GluI GCNGC 3 cut(s) 138, 218, 227
HaeII RGCGCY 1 cut(s) 458
HaeIII GGCC 2 cut(s) 169, 408
HapII CCGG 1 cut(s) 161
HhaI GCGC 1 cut(s) 457
Hin1II CATG 2 cut(s) 413, 419
Hin6I GCGC 1 cut(s) 455
HinP1I GCGC 1 cut(s) 455
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HinfI GANTC 2 cut(s) 92, 262
HpaII CCGG 1 cut(s) 161
Hpy166II GTNNAC 3 cut(s) 212, 316, 429
Hpy188I TCNGA 1 cut(s) 252
Hpy8I GTNNAC 3 cut(s) 212, 316, 429
HpyAV CCTTC 2 cut(s) 148, 494
HpyCH4III ACNGT 3 cut(s) 130, 283, 338
HpyCH4IV ACGT 1 cut(s) 497
HpyCH4V TGCA 4 cut(s) 140, 212, 217, 419
HpyF10VI GCNNNNNNNGC 4 cut(s) 223, 226, 362, 405
HpyF3I CTNAG 1 cut(s) 296
HpySE526I ACGT 1 cut(s) 497
Hsp92II CATG 2 cut(s) 413, 419
HspAI GCGC 1 cut(s) 455
Kzo9I GATC 2 cut(s) 358, 488
LmnI GCTCC 1 cut(s) 276
LpnPI CCDG 3 cut(s) 174, 223, 399
Lsp1109I GCAGC 2 cut(s) 124, 229
LweI GCATC 2 cut(s) 370, 384
MaeI CTAG 1 cut(s) 432
MaeII ACGT 1 cut(s) 497
MaeIII GTNAC 3 cut(s) 79, 101, 130
MalI GATC 2 cut(s) 360, 490
MboI GATC 2 cut(s) 358, 488
MboII GAAGA 4 cut(s) 17, 31, 199, 202
MfeI CAATTG 1 cut(s) 141
MhlI GDGCHC 2 cut(s) 214, 281
MlsI TGGCCA 2 cut(s) 169, 408
MluCI AATT 1 cut(s) 141
MluNI TGGCCA 2 cut(s) 169, 408
MnlI CCTC 4 cut(s) 80, 144, 248, 291
Mox20I TGGCCA 2 cut(s) 169, 408
MscI TGGCCA 2 cut(s) 169, 408
MseI TTAA 1 cut(s) 204
MslI CAYNNNNRTG 2 cut(s) 163, 414
Msp20I TGGCCA 2 cut(s) 169, 408
MspA1I CMGCKG 1 cut(s) 399
MspI CCGG 1 cut(s) 161
MunI CAATTG 1 cut(s) 141
MvnI CGCG 1 cut(s) 425
MwoI GCNNNNNNNGC 4 cut(s) 223, 226, 362, 405
NdeII GATC 2 cut(s) 358, 488
NlaIII CATG 2 cut(s) 413, 419
NlaIV GGNNCC 2 cut(s) 159, 481
NmuCI GTSAC 1 cut(s) 130
NspI RCATGY 1 cut(s) 419
PaeI GCATGC 1 cut(s) 419
PcsI WCGNNNNNNNCGW 1 cut(s) 248
PfeI GAWTC 2 cut(s) 92, 262
PflFI GACNNNGTC 1 cut(s) 304
PkrI GCNGC 3 cut(s) 139, 219, 228
Ple19I CGATCG 1 cut(s) 491
Ppu21I YACGTR 1 cut(s) 498
PspN4I GGNNCC 2 cut(s) 159, 481
PspPI GGNCC 2 cut(s) 300, 479
PstI CTGCAG 1 cut(s) 219
PstNI CAGNNNCTG 1 cut(s) 134
PsyI GACNNNGTC 1 cut(s) 304
PvuI CGATCG 1 cut(s) 491
RsaI GTAC 3 cut(s) 147, 340, 428
RsaNI GTAC 3 cut(s) 146, 339, 427
RseI CAYNNNNRTG 2 cut(s) 163, 414
SaqAI TTAA 1 cut(s) 204
SatI GCNGC 3 cut(s) 138, 218, 227
Sau3AI GATC 2 cut(s) 358, 488
Sau96I GGNCC 2 cut(s) 300, 479
SduI GDGCHC 2 cut(s) 214, 281
SetI ASST 2 cut(s) 119, 500
SfaNI GCATC 2 cut(s) 370, 384
SfcI CTRYAG 1 cut(s) 215
SinI GGWCC 2 cut(s) 300, 479
SmiMI CAYNNNNRTG 2 cut(s) 163, 414
SphI GCATGC 1 cut(s) 419
Sse9I AATT 1 cut(s) 141
SsiI CCGC 3 cut(s) 226, 274, 399
SspMI CTAG 1 cut(s) 432
StyI CCWWGG 1 cut(s) 441
TaaI ACNGT 3 cut(s) 130, 283, 338
TaiI ACGT 1 cut(s) 500
TaqI TCGA 4 cut(s) 72, 378, 487, 491
TasI AATT 1 cut(s) 141
TatI WGTACW 1 cut(s) 145
TauI GCSGC 1 cut(s) 229
TfiI GAWTC 2 cut(s) 92, 262
Tru1I TTAA 1 cut(s) 204
Tru9I TTAA 1 cut(s) 204
TscAI CASTG 4 cut(s) 112, 135, 219, 288
TseFI GTSAC 1 cut(s) 130
TseI GCWGC 2 cut(s) 137, 217
Tsp45I GTSAC 1 cut(s) 130
TspDTI ATGAA 2 cut(s) 5, 209
TspRI CASTG 4 cut(s) 112, 135, 219, 288
Tth111I GACNNNGTC 1 cut(s) 304
VneI GTGCAC 1 cut(s) 210
VpaK11BI GGWCC 2 cut(s) 300, 479
XceI RCATGY 1 cut(s) 419
XcmI CCANNNNNNNNNTGG 2 cut(s) 118, 448
XspI CTAG 1 cut(s) 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.