pycom12g04720

Colon cancer-associated protein Mic1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0012938
Physical Location & Seq
Reverse (-)
4621188 .. 4625901
4714 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1875 bp
ATGGATTTCAATCTCAGTGGGCTGGACTTGTTTGCTTACAATTCTGAGTCAAAATCACTCCAATTGGTTGAGACGAGGAAATTGAATGTGAGTTGGTATGTTTACACACATGAAAGTCGCATGGTTCTTCTTGCTTCAGGAATGCAGTGCAAAACCTTCAATGGTTTTCAGCTCTCATCTGCAGGCATTATTCGTCTGCCAAAGTTTGAGATGGCAATGGCTAAATCTGAGGCTAACAATAAACCTGTCCTTGCTGCTGAAGATATTTTTATAGTCACTGTCTATGGCAGAATATACTGTCTGCAAGTTGATAGGATTGCAATGCTCCTTCACTCTTACAGGTTTTACCGTGATGTGGTTGTACAACAGGGTTCTTTGCCAATTTATTCAAGCAAGGTTGCTGTGAGTGTGGTTGATAATGTATTGCTTGTGCATCAAGTTGATGCGAAGGTTGTTATACTATATGATATATTTGCAGATTCTCGGGCACCCATATCAGCCCCACTTCCTCTACTATTCAGGGGTTTCCCCAGGTCTAATTCTTCTTCCCTGAGATCAAATAGAGAAGATAGTGAAAGTTCAGAAGTTAATGTTTTAAGTGACCATGAAGCAATCGTCTACGGAGATAATTGGACTTTTCTCGTTCCTGATCTCATCTGTGATGTTGCTAATCAACTTTTGTGGAAGATTCATTTAGATTTGGAGGCAATTTCTGCCAGTAGCTCAGAAGTACCATCAGTACTTGAATTCTTGCAACGGCGGAAGTTAGAAGCCAATAAGGCTAAACAACTGTGCTTATCAATAGCACGCACTGTTATTCTAGAACGCAGACCTGTGTCTACAGTGTCTCGGGCATTAGACGTTTTAGTTTCCTCCTACTCCCACTCTATTAAGACAGGCACCTATCTTAAGGGAATAAAATCTGCAAAAACATTGCCTTCTGGTGAGCCACAGACGAGTGGCCCTAGATCTAGTGCTGATGTATCTTCTAGAAGAGTAGACACAGTTGGGAAGTCCATTAAATATGAATCTGCTGCTGGATTGGACAGTGAATCGCCTAACAGATTTTTAACCTATTCAAATTCCGACTCTGAGGATAGTACAAGCTTTGAAGCGCCAAGGGCCACTTCAAATAACTCTCAATTGTTCGATGGTAAAATGGACAGGGGGAAGTTAACGGGTGCTGAAACTTCTGGCGGTGAAATTCGGTCTTCATCTTTGCAATATCAGGTTCTTAGATCCGGCAACAGCCCATTGGATGCTAATGCGTCGGAGCAGCAAGAGTCCCAACTGACCTCTCCAGTAATTTCATCTTATGAAATGTACAGTTTTGTGTTCGCTCCTGTTGAGGAAGAGATTATTGGAGAACCTTCATACTTGGTTGCCATCATTGTTGAGTTTCTTCGTAGTGCTAATATTGAAAAGGTTGAAGTCCATCCTAATCTCTATGTCCTGACCGTACAATTACTATCACGCAGTGAGCGATATGCAGAACTTGGGCAGTTTGTCTTAAACAAGATTCTTGAACCTTCTAAAGAAGTAGCAATGCAACTCCTAGAGTCTGGCCGTCAGAACTCTCGAACAAGGAAGTTGGGTTTAGATATGCTGAGGCAGCTTTCTTTACACCATGACTATGTACTGTTACTAGTGCAAGATGGATATTACCTTGAAGCATTACGCTATGCACGGAAGTACAAGGTCAGTACCATTCGACCTTCGCTGTTTCTCGAGTCAGCATGTACCTCCAATGACTTGCAAAATTTAGCTGCAGTCCTTAGATTCTTTTCGGATTTCATTCCTGGATTCAGAGACACATCGGATCACGACACTTACTACCGCATTCTCAGCGAGAGGAACTCATCCATTGCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mic1

Protein Analysis

625

Amino Acids

69.72

Weight (kDa)

6.03

Isoelectric Point (pI)

50.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RMC1_C PF07035 415 - 597 2.9e-45 Regulator of MON1-CCZ1 complex, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012938)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12010
fragaria_vesca FvH4_6g21770
malus_domestica MD12G1054100.v1.1 MD14G1053000.v1.1
prunus_persica Prupe.7G082700_v2.0.a1
pyrus_communis pycom12g04720 pycom14g04400
rosa_chinensis RchiOBHm_Chr3g0479121
rosa_laevigata RLG00000023605
rosa_multiflora Rmu_sc0000038.1_g000003
rosa_roxburghii Rroxscaffold_6G00402710
rosa_rugosa Rorug03G0171200 Rorug03G0171300
rosa_samantha Rh3AG222300 Rh3BG254800 Rh3CG250500 Rh3DG246800
rosa_wichuraiana Rw3G019930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 487, 899
AccI GTMKAC 3 cut(s) 618, 839, 999
AciI CCGC 3 cut(s) 760, 1197, 1837
AclWI GGATC 2 cut(s) 1233, 1827
AcoI YGGCCR 1 cut(s) 1564
AcsI RAATTY 4 cut(s) 746, 1081, 1203, 1759
AcuI CTGAAG 2 cut(s) 120, 279
AdeI CACNNNGTG 1 cut(s) 1478
AfiI CCNNNNNNNGG 1 cut(s) 355
AflII CTTAAG 1 cut(s) 908
AhlI ACTAGT 1 cut(s) 1645
AjnI CCWGG 2 cut(s) 530, 1798
AjuI GAANNNNNNNTTGG 4 cut(s) 1111, 1143, 1344, 1376
AluBI AGCT 5 cut(s) 172, 723, 1107, 1615, 1766
AluI AGCT 5 cut(s) 172, 723, 1107, 1615, 1766
Alw26I GTCTC 3 cut(s) 65, 852, 1803
AlwI GGATC 2 cut(s) 1233, 1827
Ama87I CYCGRG 3 cut(s) 483, 849, 1727
AoxI GGCC 3 cut(s) 961, 1122, 1564
ApeKI GCWGC 6 cut(s) 254, 1034, 1276, 1612, 1766, 1868
ApoI RAATTY 4 cut(s) 746, 1081, 1203, 1759
AspLEI GCGC 1 cut(s) 1117
AspS9I GGNCC 2 cut(s) 962, 1122
AsuHPI GGTGA 2 cut(s) 956, 1211
AvaI CYCGRG 3 cut(s) 483, 849, 1727
BaeGI GKGCMC 1 cut(s) 490
BanI GGYRCC 2 cut(s) 487, 899
BbsI GAAGAC 1 cut(s) 1203
BbvCI CCTCAGC 1 cut(s) 1607
BbvI GCAGC 6 cut(s) 241, 1021, 1288, 1624, 1753, 1855
BccI CCATC 6 cut(s) 205, 742, 1145, 1394, 1443, 1649
BceAI ACGGC 2 cut(s) 773, 1551
BciT130I CCWGG 2 cut(s) 532, 1800
BcoDI GTCTC 3 cut(s) 65, 852, 1803
BcuI ACTAGT 1 cut(s) 1645
BfaI CTAG 6 cut(s) 821, 966, 972, 990, 1556, 1646
BfmI CTRYAG 3 cut(s) 180, 840, 1767
BfoI RGCGCY 1 cut(s) 1118
BfrI CTTAAG 1 cut(s) 908
BglI GCCNNNNNGGC 1 cut(s) 779
BglII AGATCT 1 cut(s) 968
BisI GCNGC 6 cut(s) 255, 1035, 1277, 1613, 1767, 1869
BlsI GCNGC 6 cut(s) 256, 1036, 1278, 1614, 1768, 1870
BmcAI AGTACT 1 cut(s) 741
Bme1390I CCNGG 2 cut(s) 532, 1800
BmeT110I CYCGRG 3 cut(s) 483, 849, 1727
BmgT120I GGNCC 2 cut(s) 962, 1122
BmiI GGNNCC 2 cut(s) 489, 901
BmrFI CCNGG 2 cut(s) 532, 1800
BmsI GCATC 3 cut(s) 433, 442, 1249
BoxI GACNNNNGTC 1 cut(s) 835
BpiI GAAGAC 1 cut(s) 1203
BplI GAGNNNNNCTC 2 cut(s) 1841, 1873
BpmI CTGGAG 1 cut(s) 1284
Bpu10I CCTNAGC 1 cut(s) 1607
BsaBI GATNNNNATC 1 cut(s) 9
BsaJI CCNNGG 2 cut(s) 530, 1118
Bsc4I CCNNNNNNNGG 1 cut(s) 355
Bse1I ACTGG 2 cut(s) 717, 1301
Bse3DI GCAATG 5 cut(s) 222, 327, 932, 1551, 1863
Bse8I GATNNNNATC 1 cut(s) 9
BseBI CCWGG 2 cut(s) 532, 1800
BseDI CCNNGG 2 cut(s) 530, 1118
BseGI GGATG 3 cut(s) 1264, 1435, 1859
BseJI GATNNNNATC 1 cut(s) 9
BseLI CCNNNNNNNGG 1 cut(s) 355
BseMI GCAATG 5 cut(s) 222, 327, 932, 1551, 1863
BseMII CTCAG 8 cut(s) 28, 36, 219, 542, 738, 1083, 1598, 1858
BseNI ACTGG 2 cut(s) 717, 1301
BseSI GKGCMC 1 cut(s) 490
BseXI GCAGC 6 cut(s) 241, 1021, 1288, 1624, 1753, 1855
BshFI GGCC 3 cut(s) 963, 1124, 1566
BshNI GGYRCC 2 cut(s) 487, 899
BsiHKCI CYCGRG 3 cut(s) 483, 849, 1727
BsiSI CCGG 1 cut(s) 1242
BslFI GGGAC 1 cut(s) 1270
BslI CCNNNNNNNGG 1 cut(s) 355
BsmAI GTCTC 3 cut(s) 65, 852, 1803
BsmBI CGTCTC 1 cut(s) 65
BsmFI GGGAC 1 cut(s) 1270
BsmI GAATGC 2 cut(s) 147, 1839
BsnI GGCC 3 cut(s) 963, 1124, 1566
BsoBI CYCGRG 3 cut(s) 483, 849, 1727
Bsp1286I GDGCHC 1 cut(s) 490
Bsp1407I TGTACA 2 cut(s) 361, 1323
Bsp143I GATC 5 cut(s) 554, 649, 968, 1238, 1819
BspACI CCGC 3 cut(s) 760, 1197, 1837
BspANI GGCC 3 cut(s) 963, 1124, 1566
BspCNI CTCAG 8 cut(s) 27, 37, 220, 543, 737, 1084, 1599, 1857
BspLI GGNNCC 2 cut(s) 489, 901
BspMAI CTGCAG 2 cut(s) 184, 1771
BspPI GGATC 2 cut(s) 1233, 1827
BspT107I GGYRCC 2 cut(s) 487, 899
BspTI CTTAAG 1 cut(s) 908
BsrDI GCAATG 5 cut(s) 222, 327, 932, 1551, 1863
BsrGI TGTACA 2 cut(s) 361, 1323
BsrI ACTGG 2 cut(s) 717, 1301
BssECI CCNNGG 2 cut(s) 530, 1118
BssMI GATC 5 cut(s) 554, 649, 968, 1238, 1819
BssT1I CCWWGG 1 cut(s) 1118
Bst2UI CCWGG 2 cut(s) 532, 1800
Bst6I CTCTTC 2 cut(s) 988, 1347
BstAFI CTTAAG 1 cut(s) 908
BstAPI GCANNNNNTGC 1 cut(s) 713
BstAUI TGTACA 2 cut(s) 361, 1323
BstC8I GCNNGC 2 cut(s) 184, 808
BstF5I GGATG 3 cut(s) 1264, 1435, 1859
BstH2I RGCGCY 1 cut(s) 1118
BstHHI GCGC 1 cut(s) 1117
BstKTI GATC 5 cut(s) 557, 652, 971, 1241, 1822
BstMAI GTCTC 3 cut(s) 65, 852, 1803
BstMBI GATC 5 cut(s) 554, 649, 968, 1238, 1819
BstMWI GCNNNNNNNGC 5 cut(s) 713, 779, 1121, 1612, 1845
BstNI CCWGG 2 cut(s) 532, 1800
BstNSI RCATGY 1 cut(s) 1740
BstPAI GACNNNNGTC 1 cut(s) 835
BstSCI CCNGG 2 cut(s) 530, 1798
BstSFI CTRYAG 3 cut(s) 180, 840, 1767
BstSLI GKGCMC 1 cut(s) 490
BstV1I GCAGC 6 cut(s) 241, 1021, 1288, 1624, 1753, 1855
BstV2I GAAGAC 1 cut(s) 1203
BstX2I RGATCY 2 cut(s) 968, 1238
BstYI RGATCY 2 cut(s) 968, 1238
BsuRI GGCC 3 cut(s) 963, 1124, 1566
BtsCI GGATG 3 cut(s) 1264, 1435, 1859
BtsI GCAGTG 2 cut(s) 152, 1483
BtsIMutI CAGTG 7 cut(s) 22, 152, 276, 810, 849, 1054, 1483
Cac8I GCNNGC 2 cut(s) 184, 808
CfoI GCGC 1 cut(s) 1117
Cfr13I GGNCC 2 cut(s) 962, 1122
CseI GACGC 1 cut(s) 1257
CspCI CAANNNNNGTGG 3 cut(s) 33, 662, 697
CviAII CATG 5 cut(s) 110, 121, 605, 1628, 1737
DpnI GATC 5 cut(s) 556, 651, 970, 1240, 1821
DpnII GATC 5 cut(s) 554, 649, 968, 1238, 1819
DraIII CACNNNGTG 1 cut(s) 1478
EaeI YGGCCR 1 cut(s) 1564
Eam1104I CTCTTC 2 cut(s) 988, 1347
EarI CTCTTC 2 cut(s) 988, 1347
EciI GGCGGA 1 cut(s) 775
Eco130I CCWWGG 1 cut(s) 1118
Eco57I CTGAAG 2 cut(s) 120, 279
Eco88I CYCGRG 3 cut(s) 483, 849, 1727
EcoRI GAATTC 1 cut(s) 746
EcoRII CCWGG 2 cut(s) 530, 1798
EcoT14I CCWWGG 1 cut(s) 1118
ErhI CCWWGG 1 cut(s) 1118
Esp3I CGTCTC 1 cut(s) 65
FaeI CATG 5 cut(s) 113, 124, 608, 1631, 1740
FalI AAGNNNNNCTT 2 cut(s) 1111, 1143
FaqI GGGAC 1 cut(s) 1270
FatI CATG 5 cut(s) 109, 120, 604, 1627, 1736
FblI GTMKAC 3 cut(s) 618, 839, 999
Fnu4HI GCNGC 6 cut(s) 255, 1035, 1277, 1613, 1767, 1869
FokI GGATG 3 cut(s) 1271, 1422, 1846
Fsp4HI GCNGC 6 cut(s) 255, 1035, 1277, 1613, 1767, 1869
FspBI CTAG 6 cut(s) 821, 966, 972, 990, 1556, 1646
GlaI GCGC 1 cut(s) 1116
GluI GCNGC 6 cut(s) 255, 1035, 1277, 1613, 1767, 1869
GsuI CTGGAG 1 cut(s) 1284
HaeII RGCGCY 1 cut(s) 1118
HaeIII GGCC 3 cut(s) 963, 1124, 1566
HapII CCGG 1 cut(s) 1242
HgaI GACGC 1 cut(s) 1257
HhaI GCGC 1 cut(s) 1117
Hin1II CATG 5 cut(s) 113, 124, 608, 1631, 1740
Hin6I GCGC 1 cut(s) 1115
HinP1I GCGC 1 cut(s) 1115
HincII GTYRAC 1 cut(s) 1176
HindII GTYRAC 1 cut(s) 1176
HindIII AAGCTT 1 cut(s) 1105
HpaI GTTAAC 1 cut(s) 1176
HpaII CCGG 1 cut(s) 1242
HphI GGTGA 2 cut(s) 956, 1211
Hpy166II GTNNAC 5 cut(s) 103, 619, 840, 1000, 1176
Hpy188III TCNNGA 9 cut(s) 138, 647, 821, 990, 1453, 1523, 1578, 1727, 1823
Hpy8I GTNNAC 5 cut(s) 103, 619, 840, 1000, 1176
Hpy99I CGWCG 1 cut(s) 1273
HpyAV CCTTC 7 cut(s) 166, 338, 442, 948, 1380, 1539, 1725
HpyCH4IV ACGT 1 cut(s) 861
HpyF10VI GCNNNNNNNGC 5 cut(s) 713, 779, 1121, 1612, 1845
HpySE526I ACGT 1 cut(s) 861
Hsp92II CATG 5 cut(s) 113, 124, 608, 1631, 1740
HspAI GCGC 1 cut(s) 1115
KspAI GTTAAC 1 cut(s) 1176
Kzo9I GATC 5 cut(s) 554, 649, 968, 1238, 1819
LmnI GCTCC 3 cut(s) 330, 1273, 1345
Lsp1109I GCAGC 6 cut(s) 241, 1021, 1288, 1624, 1753, 1855
LweI GCATC 3 cut(s) 433, 442, 1249
MaeI CTAG 6 cut(s) 821, 966, 972, 990, 1556, 1646
MaeII ACGT 1 cut(s) 861
MaeIII GTNAC 3 cut(s) 274, 599, 1641
MalI GATC 5 cut(s) 556, 651, 970, 1240, 1821
MboI GATC 5 cut(s) 554, 649, 968, 1238, 1819
MfeI CAATTG 2 cut(s) 62, 1142
MflI RGATCY 2 cut(s) 968, 1238
MhlI GDGCHC 1 cut(s) 490
MlyI GAGTC 5 cut(s) 56, 1082, 1292, 1568, 1739
MmeI TCCRAC 2 cut(s) 1110, 1251
MseI TTAA 8 cut(s) 588, 596, 891, 909, 1020, 1070, 1175, 1511
MslI CAYNNNNRTG 1 cut(s) 1632
MspCI CTTAAG 1 cut(s) 908
MspI CCGG 1 cut(s) 1242
MspR9I CCNGG 2 cut(s) 532, 1800
MunI CAATTG 2 cut(s) 62, 1142
Mva1269I GAATGC 2 cut(s) 147, 1839
MvaI CCWGG 2 cut(s) 532, 1800
MwoI GCNNNNNNNGC 5 cut(s) 713, 779, 1121, 1612, 1845
NdeII GATC 5 cut(s) 554, 649, 968, 1238, 1819
NlaIII CATG 5 cut(s) 113, 124, 608, 1631, 1740
NlaIV GGNNCC 2 cut(s) 489, 901
NmuCI GTSAC 2 cut(s) 274, 599
NspI RCATGY 1 cut(s) 1740
PaeR7I CTCGAG 1 cut(s) 1727
PcsI WCGNNNNNNNCGW 1 cut(s) 1480
PctI GAATGC 2 cut(s) 147, 1839
PfeI GAWTC 7 cut(s) 479, 688, 1028, 1052, 1519, 1779, 1803
PfoI TCCNGGA 1 cut(s) 1798
PkrI GCNGC 6 cut(s) 256, 1036, 1278, 1614, 1768, 1870
PleI GAGTC 5 cut(s) 55, 1082, 1291, 1567, 1738
PpsI GAGTC 5 cut(s) 55, 1082, 1291, 1567, 1738
PshAI GACNNNNGTC 1 cut(s) 835
Psp6I CCWGG 2 cut(s) 530, 1798
PspGI CCWGG 2 cut(s) 530, 1798
PspN4I GGNNCC 2 cut(s) 489, 901
PspPI GGNCC 2 cut(s) 962, 1122
PstI CTGCAG 2 cut(s) 184, 1771
PsuI RGATCY 2 cut(s) 968, 1238
RseI CAYNNNNRTG 1 cut(s) 1632
SaqAI TTAA 8 cut(s) 588, 596, 891, 909, 1020, 1070, 1175, 1511
SatI GCNGC 6 cut(s) 255, 1035, 1277, 1613, 1767, 1869
Sau3AI GATC 5 cut(s) 554, 649, 968, 1238, 1819
Sau96I GGNCC 2 cut(s) 962, 1122
ScaI AGTACT 1 cut(s) 741
SchI GAGTC 5 cut(s) 56, 1082, 1292, 1568, 1739
ScrFI CCNGG 2 cut(s) 532, 1800
SduI GDGCHC 1 cut(s) 490
SfaNI GCATC 3 cut(s) 433, 442, 1249
SfcI CTRYAG 3 cut(s) 180, 840, 1767
Sfr274I CTCGAG 1 cut(s) 1727
SlaI CTCGAG 1 cut(s) 1727
SmiMI CAYNNNNRTG 1 cut(s) 1632
SmlI CTYRAG 2 cut(s) 908, 1727
SmoI CTYRAG 2 cut(s) 908, 1727
SpeI ACTAGT 1 cut(s) 1645
SsiI CCGC 3 cut(s) 760, 1197, 1837
SspI AATATT 1 cut(s) 1417
SspMI CTAG 6 cut(s) 821, 966, 972, 990, 1556, 1646
StyD4I CCNGG 2 cut(s) 530, 1798
StyI CCWWGG 1 cut(s) 1118
TaiI ACGT 1 cut(s) 864
TaqI TCGA 4 cut(s) 1149, 1579, 1711, 1728
TaqII GACCGA 1 cut(s) 1197
TatI WGTACW 6 cut(s) 361, 739, 1100, 1323, 1636, 1692
TfiI GAWTC 7 cut(s) 479, 688, 1028, 1052, 1519, 1779, 1803
Tru1I TTAA 8 cut(s) 588, 596, 891, 909, 1020, 1070, 1175, 1511
Tru9I TTAA 8 cut(s) 588, 596, 891, 909, 1020, 1070, 1175, 1511
TscAI CASTG 7 cut(s) 22, 152, 283, 817, 849, 1054, 1483
TseFI GTSAC 2 cut(s) 274, 599
TseI GCWGC 6 cut(s) 254, 1034, 1276, 1612, 1766, 1868
Tsp45I GTSAC 2 cut(s) 274, 599
TspDTI ATGAA 9 cut(s) 126, 621, 680, 1041, 1203, 1299, 1332, 1362, 1783
TspGWI ACGGA 2 cut(s) 636, 1702
TspRI CASTG 7 cut(s) 22, 152, 283, 817, 849, 1054, 1483
Vha464I CTTAAG 1 cut(s) 908
XapI RAATTY 4 cut(s) 746, 1081, 1203, 1759
XbaI TCTAGA 2 cut(s) 820, 989
XceI RCATGY 1 cut(s) 1740
XhoI CTCGAG 1 cut(s) 1727
XmiI GTMKAC 3 cut(s) 618, 839, 999
XspI CTAG 6 cut(s) 821, 966, 972, 990, 1556, 1646
ZrmI AGTACT 1 cut(s) 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.