Rorug03G0171300
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0002243
Physical Location & Seq
Forward (+)
14468461 .. 14470836
2376 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0171300.1

Sequence Viewer

Length: 816 bp
ATGGCTGAAAAGAATAAGAGCAAAGTTGTGTGCTCAGATGGTGATGATCACAGTGATGCTGAAGGAAGCAAAAACACATTGGATGACTTGACTCTCCCGCTACAGAAACTCAACCTTGGCCCAGAAAAGAAGAAGAAGAAGAAGCTTCTGGTTATTGGTCTTGGAGGCTTGCTTTGTCATAGAGTATATCGCACTGAAAAAATCTATATCCCAAATTATCGCCATCCGGATGCAGCATATGGAAACTACAAGGTTTACAAGAGGCCTTACTGTGAAGAATTTATGAAATTTTGTCTGGAAAGATTTGAAGTGGGAATATGGTCTTCTGCAAGGGAATGGTACTTGGACAATGCCTTGGATTGTGTGATGAAAGGGTTGGGGAGGAAGTTGTTGTTTGCTTGGGATCAAGAGGAGTGTACTGATTCGGGGTTCAAAACCTTGGAGAACCAAAAGAAGCCCCTCTTTCTGAAAGAGTTGAAGAAAATATGGGAAAACAAGGGTTTCAAAGCCTCAGTTTCATCTTCCATGGGGAAATACTCTTCATTGAACACCTTGTTAATTGATGATAAGGCTTACAAGGCTCTACTAAATCCTGCTCACACAGCCATCTTTCCTCCTGAATACAAGGTTGGCCAAGTTGGTGACAAGGCTTTAGGTCCTAATGGTGAGTTGAGGTTTTACTTGGAGGGACTTGCAGATGCCGATGATGTTACTTCTTATGTTAAAGATCATCCATTTGGACAGCCAGCAATAACAACTACTCACTCTGATTGGGATTTCTATTCAAAGATCATTACTCATTTCCAGAAAGATTAA

Protein Analysis

271

Amino Acids

30.93

Weight (kDa)

8.34

Isoelectric Point (pI)

22.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 48 - 237 1.3e-16 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 226
AciI CCGC 1 cut(s) 98
AclWI GGATC 1 cut(s) 411
AcoI YGGCCR 1 cut(s) 631
AcsI RAATTY 2 cut(s) 278, 287
AcuI CTGAAG 1 cut(s) 81
AfaI GTAC 2 cut(s) 341, 418
AgsI TTSAA 6 cut(s) 308, 433, 478, 505, 547, 786
AjuI GAANNNNNNNTTGG 2 cut(s) 612, 644
AluBI AGCT 1 cut(s) 145
AluI AGCT 1 cut(s) 145
Alw21I GWGCWC 1 cut(s) 35
AlwI GGATC 1 cut(s) 411
Aor13HI TCCGGA 1 cut(s) 226
AoxI GGCC 3 cut(s) 118, 263, 631
ApeKI GCWGC 1 cut(s) 233
ApoI RAATTY 2 cut(s) 278, 287
AspS9I GGNCC 2 cut(s) 119, 656
AsuHPI GGTGA 3 cut(s) 53, 653, 677
AvaII GGWCC 1 cut(s) 656
BalI TGGCCA 1 cut(s) 633
BbsI GAAGAC 1 cut(s) 315
Bbv12I GWGCWC 1 cut(s) 35
BbvI GCAGC 1 cut(s) 245
BccI CCATC 3 cut(s) 32, 231, 614
BclI TGATCA 1 cut(s) 46
BfmI CTRYAG 1 cut(s) 101
BisI GCNGC 1 cut(s) 234
BlsI GCNGC 1 cut(s) 235
Bme18I GGWCC 1 cut(s) 656
BmgT120I GGNCC 2 cut(s) 119, 656
BmsI GCATC 3 cut(s) 46, 220, 688
BpiI GAAGAC 1 cut(s) 315
BsaJI CCNNGG 4 cut(s) 115, 354, 438, 525
BsaWI WCCGGW 1 cut(s) 226
BseAI TCCGGA 1 cut(s) 226
BseDI CCNNGG 4 cut(s) 115, 354, 438, 525
BseGI GGATG 4 cut(s) 88, 223, 235, 730
BseMII CTCAG 2 cut(s) 48, 525
BseRI GAGGAG 1 cut(s) 425
BseXI GCAGC 1 cut(s) 245
BshFI GGCC 3 cut(s) 120, 265, 633
BsiHKAI GWGCWC 1 cut(s) 35
BsiSI CCGG 1 cut(s) 227
BslFI GGGAC 1 cut(s) 702
BsmFI GGGAC 1 cut(s) 702
BsnI GGCC 3 cut(s) 120, 265, 633
Bsp1286I GDGCHC 1 cut(s) 35
Bsp13I TCCGGA 1 cut(s) 226
Bsp143I GATC 4 cut(s) 46, 403, 727, 789
Bsp19I CCATGG 1 cut(s) 525
BspACI CCGC 1 cut(s) 98
BspANI GGCC 3 cut(s) 120, 265, 633
BspCNI CTCAG 2 cut(s) 47, 524
BspEI TCCGGA 1 cut(s) 226
BspPI GGATC 1 cut(s) 411
BssECI CCNNGG 4 cut(s) 115, 354, 438, 525
BssMI GATC 4 cut(s) 46, 403, 727, 789
BssT1I CCWWGG 4 cut(s) 115, 354, 438, 525
Bst4CI ACNGT 2 cut(s) 53, 272
Bst6I CTCTTC 1 cut(s) 544
BstC8I GCNNGC 2 cut(s) 170, 747
BstDEI CTNAG 2 cut(s) 34, 511
BstDSI CCRYGG 1 cut(s) 525
BstF5I GGATG 4 cut(s) 88, 223, 235, 730
BstKTI GATC 4 cut(s) 49, 406, 730, 792
BstMBI GATC 4 cut(s) 46, 403, 727, 789
BstMWI GCNNNNNNNGC 2 cut(s) 578, 602
BstSFI CTRYAG 1 cut(s) 101
BstV1I GCAGC 1 cut(s) 245
BstV2I GAAGAC 1 cut(s) 315
BsuRI GGCC 3 cut(s) 120, 265, 633
BtgI CCRYGG 1 cut(s) 525
BtsCI GGATG 4 cut(s) 88, 223, 235, 730
BtsIMutI CAGTG 2 cut(s) 58, 192
Cac8I GCNNGC 2 cut(s) 170, 747
Cfr13I GGNCC 2 cut(s) 119, 656
Csp6I GTAC 2 cut(s) 340, 417
CviAII CATG 1 cut(s) 526
CviQI GTAC 2 cut(s) 340, 417
DdeI CTNAG 2 cut(s) 34, 511
DpnI GATC 4 cut(s) 48, 405, 729, 791
DpnII GATC 4 cut(s) 46, 403, 727, 789
EaeI YGGCCR 1 cut(s) 631
Eam1104I CTCTTC 1 cut(s) 544
EarI CTCTTC 1 cut(s) 544
Eco130I CCWWGG 4 cut(s) 115, 354, 438, 525
Eco147I AGGCCT 1 cut(s) 265
Eco47I GGWCC 1 cut(s) 656
Eco57I CTGAAG 1 cut(s) 81
EcoO109I RGGNCCY 1 cut(s) 656
EcoT14I CCWWGG 4 cut(s) 115, 354, 438, 525
ErhI CCWWGG 4 cut(s) 115, 354, 438, 525
FaeI CATG 1 cut(s) 529
FalI AAGNNNNNCTT 2 cut(s) 446, 478
FaqI GGGAC 1 cut(s) 702
FatI CATG 1 cut(s) 525
FauI CCCGC 1 cut(s) 105
FauNDI CATATG 1 cut(s) 238
FbaI TGATCA 1 cut(s) 46
Fnu4HI GCNGC 1 cut(s) 234
FokI GGATG 4 cut(s) 95, 210, 242, 717
Fsp4HI GCNGC 1 cut(s) 234
GluI GCNGC 1 cut(s) 234
HaeIII GGCC 3 cut(s) 120, 265, 633
HapII CCGG 1 cut(s) 227
Hin1II CATG 1 cut(s) 529
HindIII AAGCTT 1 cut(s) 143
HinfI GANTC 2 cut(s) 91, 422
HpaII CCGG 1 cut(s) 227
HphI GGTGA 3 cut(s) 53, 653, 677
Hpy166II GTNNAC 2 cut(s) 256, 417
Hpy188I TCNGA 3 cut(s) 37, 468, 769
Hpy188III TCNNGA 5 cut(s) 227, 296, 407, 617, 805
Hpy8I GTNNAC 2 cut(s) 256, 417
HpyAV CCTTC 1 cut(s) 56
HpyCH4III ACNGT 2 cut(s) 53, 272
HpyCH4V TGCA 3 cut(s) 233, 329, 695
HpyF10VI GCNNNNNNNGC 2 cut(s) 578, 602
HpyF3I CTNAG 2 cut(s) 34, 511
Hsp92II CATG 1 cut(s) 529
Kpn2I TCCGGA 1 cut(s) 226
Ksp22I TGATCA 1 cut(s) 46
Kzo9I GATC 4 cut(s) 46, 403, 727, 789
LpnPI CCDG 7 cut(s) 134, 135, 240, 281, 606, 630, 759
Lsp1109I GCAGC 1 cut(s) 245
LweI GCATC 3 cut(s) 46, 220, 688
MaeIII GTNAC 2 cut(s) 641, 709
MalI GATC 4 cut(s) 48, 405, 729, 791
MboI GATC 4 cut(s) 46, 403, 727, 789
MboII GAAGA 9 cut(s) 142, 145, 148, 151, 287, 315, 490, 513, 531
MhlI GDGCHC 1 cut(s) 35
MlsI TGGCCA 1 cut(s) 633
MluCI AATT 4 cut(s) 214, 278, 287, 558
MluNI TGGCCA 1 cut(s) 633
MlyI GAGTC 1 cut(s) 85
MnlI CCTC 9 cut(s) 158, 255, 375, 403, 470, 520, 624, 666, 679
Mox20I TGGCCA 1 cut(s) 633
MroI TCCGGA 1 cut(s) 226
MscI TGGCCA 1 cut(s) 633
MseI TTAA 3 cut(s) 557, 723, 814
MslI CAYNNNNRTG 2 cut(s) 54, 228
Msp20I TGGCCA 1 cut(s) 633
MspI CCGG 1 cut(s) 227
MwoI GCNNNNNNNGC 2 cut(s) 578, 602
NcoI CCATGG 1 cut(s) 525
NdeI CATATG 1 cut(s) 238
NdeII GATC 4 cut(s) 46, 403, 727, 789
NlaIII CATG 1 cut(s) 529
NmuCI GTSAC 1 cut(s) 641
PceI AGGCCT 1 cut(s) 265
PfeI GAWTC 1 cut(s) 422
PkrI GCNGC 1 cut(s) 235
PleI GAGTC 1 cut(s) 85
PpsI GAGTC 1 cut(s) 85
PpuMI RGGWCCY 1 cut(s) 656
Psp5II RGGWCCY 1 cut(s) 656
PspPI GGNCC 2 cut(s) 119, 656
PspPPI RGGWCCY 1 cut(s) 656
RsaI GTAC 2 cut(s) 341, 418
RsaNI GTAC 2 cut(s) 340, 417
RseI CAYNNNNRTG 2 cut(s) 54, 228
SaqAI TTAA 3 cut(s) 557, 723, 814
SatI GCNGC 1 cut(s) 234
Sau3AI GATC 4 cut(s) 46, 403, 727, 789
Sau96I GGNCC 2 cut(s) 119, 656
SchI GAGTC 1 cut(s) 85
SduI GDGCHC 1 cut(s) 35
SetI ASST 8 cut(s) 117, 147, 255, 440, 554, 630, 658, 677
SfaNI GCATC 3 cut(s) 46, 220, 688
SfcI CTRYAG 1 cut(s) 101
SinI GGWCC 1 cut(s) 656
SmiMI CAYNNNNRTG 2 cut(s) 54, 228
Sse9I AATT 4 cut(s) 214, 278, 287, 558
SseBI AGGCCT 1 cut(s) 265
SsiI CCGC 1 cut(s) 98
StuI AGGCCT 1 cut(s) 265
StyI CCWWGG 4 cut(s) 115, 354, 438, 525
TaaI ACNGT 2 cut(s) 53, 272
TasI AATT 4 cut(s) 214, 278, 287, 558
TatI WGTACW 1 cut(s) 416
TfiI GAWTC 1 cut(s) 422
Tru1I TTAA 3 cut(s) 557, 723, 814
Tru9I TTAA 3 cut(s) 557, 723, 814
TscAI CASTG 2 cut(s) 58, 199
TseFI GTSAC 1 cut(s) 641
TseI GCWGC 1 cut(s) 233
Tsp45I GTSAC 1 cut(s) 641
TspDTI ATGAA 4 cut(s) 299, 383, 507, 531
TspRI CASTG 2 cut(s) 58, 199
VpaK11BI GGWCC 1 cut(s) 656
XapI RAATTY 2 cut(s) 278, 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.