Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3B

Orthogroup ID

OG0002243

Physical Location & Seq

Genomic Coordinates Forward (+)

24646428 .. 24659984

Gene Structure 13557 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3BG255100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3027 bp

ATGGCGTCAAAACATGGATCACGATCGAAAAAACTAGGTTCCAGCAATTCGAGAGCTGCTAATTCTCCGTCATCTTCGACTACATCGTCTTCGAAACAATATTTGGAAACTTCTATTGAAGGGCAGAGCTCTCCTGCATCTTCTTCAGCTCGAAGTAAGCCACAGTACTTTTACACGGAAAGTTTGCCACAAGATGCGGAAAGGTCCAAAGAAAATGTCACAGTGACGGTCCGCTTTCGTCCACTCAGTCCAAGGGAAATTCGCCAAGGAGAAGAAATTGCATGGTATGCAGATGGAGACACTATTCTGCGGAATGAGCATAATCCATCAATAGCTTATGCATACGATCGGGTGTTTGGCCCTACAACCACAACACGCCATGTCTATGATGTTGCTGCTCAGCATGTTGTTAGTGGTGCTATGGAAGGAGTCAACGGTACAATTTTTGCATATGGGGTCACCAGCAGCGGGAAGACTCACACAATGCATGGTGATCAAAGGTCGCCTGGAATTATACCCCTGGCTGTGAAAGATGCGTTTAGCATCATCCAAGAGACTCCAAACCGAGAGTTTCTTCTTCGTGTGTCATACTTGGAGATCTATAATGAGGTTGTTAATGACTTGTTGAATCCAGCAGGACAGAATTTACGAATTAGAGAGGATGCTCAGGGTACCTTTGTTGAAGGAATAAAGGAAGAAGTAGTGCTATCCCCAGCTCATGCCCTGTCACTTATAGCAGCTGGAGAAGAGCACAGGCATGTTGGATCAACAAATTTCAATTTACTCAGTAGCAGGAGCCATACTATATTTACACTGACAATCGAGAGTAGCCCGTGTGGGGAAAATAGTGAAGGTGAAGCTGTAAATCTGTCGCAATTGAACCTCATTGATCTGGCTGGTTCTGAGAGCTCAAAAGCTGAAACTACTGGTGTCAGACGAAAAGAAGGATCCTATATTAATAAGAGTCTGCTAACTCTAGGAACTGTTATATCAAAGTTGACGGATGGGAGGGCTACTCATATACCCTACAGGGATTCTAAACTGACCCGACTTCTCCAATCTTCGCTAAGCGGTCATGGGCGTGTCTCTCTCATCTGCACTGTTACTCCTTCATCAAGCAGTTCAGAAGAGACGCATAATACATTAAAGTTTGCACACCGGGCAAAGCACATTGAAATTCAAGCAGCACAAAATAAGATTATTGATGAGAAGTCACTTATCAAGAAATATCAAAATGAGATTCGTAATTTAAAGGAAGAGTTAGAACAGTTGAAGAAGGGTATTGTTACAGTTCCTCAACTAAAAGATGCTAGCGAAAATGATATTCTGCTTCTAAAACAAAAGTTAGAAGATGGCAAATTCAAACTGCAATCAAGATTGGAACAAGAGGAAGAAGCCAAAGCTGCTTTGTTAGGAAGAATCCAAAGGTTGACAAAGTTGATTTTGGTCTCCACAAAAGCAACACAGCCATCAAGGTTTCCTCACCGACCTGGTCTTAGAAGAAGACATTCCTTTGGGGAAGAGGAGCTTGCATATCTACCATACAAAAGGCGGGACTTGATTTTAGAGGATGAAAACATTGACTTGTTTGTTTCTCCTCTTGAGGGGAATACTGAAACTGCTGATGATACACTGAAGGAGGATAAGAAGACTCGGAAGCATGGACTGTTAAACTGGTTAAAGCTACGGAAACGAGATAGTGGTGGTGGGACTTTGACAAGCACTAGTGATAAATCCAGTGGAATGAAATCTACTAGTCAACCATCAACACCTCAAGCAGAAAATAGTAATTTTCATGCAGAATCGAGGCTTTCCCATTCTCTACTTACAGAGAGCTCTCCATCTGCTGATCTTTTGACTGATGCTATAGAGGATAGAGAGGTACCTCAGGATAAATTCCTGGGACAAGAGACACCTACGACCAGCATAAAATCAATTGATCAGATCGATCTATTAAGAGAGCAGCAAAAAATCTTGTCCGGAGAGGTAGCACTCCATTCAAGTGCACTGAAGCGTTTGTCTGAGGAGGCTGCAAAAAACCCCCAGAAGGACAGATTAAATATGGAGATGCGAAAGTTAAAAGATGAAATCAAGGCGAAGAATGAACAAATAGCTCTGCTGGAAAAGAAAATTGCCGATTCGTTGATTGTTTCACCCACCAAAATGGATCAAATTGAAATATCCCAATCTATTGCCGAAGTGGTGGCACAATTGAATGAGAAGTCCTTCGAACTTGAGGTTAAAGCTGCTGATAATCGTATTATTCAAGAGCAGCTAGAACAAAAGATCTGCGAATGCAAAGAATTGCAGGAAACAGTTGCCTCCCTGAAGCAGCAGCTCTCTGAGGCACTGGAGTTTAGAAATTTGAGTCCAATAATCAGTTCGCAAACTGACTCAAGAATCGTGCATGCGGGAGAGAATGCAGTGTTGAATCACACAAATGAAGTTTTCCTTACAGATAAGCAGGCGCTTGAGATTGAAGAACTGAAGCAGAAGGTGGCTGAAATGACAGAGTCAAAAGAGCAGTTAGAACTTCGGAACCAGAAACTGGTGGAAGAAAGTTCATATGCCAAAGGTTTAGCCTCAGCTGCTGCTGTTGAGCTCAAGGCATTATCAGAGGAAGTTGCCAAACTCATGAACCATAATGAGAGACTAGCTGCTGAGCTGGCCGCATCTAAGAACTCCCCCACCCAGCGTCGAAGTGGTAACACACTCCGGAATGGTCGAAGAGAGAGTCACGTCAAACAGAATGACCACAGTGGGCCCGTCTCAGAAATGAAGAGAGAACTGGCCATGAGCAAGGAGAGAGAGCTTTCATATGAGGCGGCTCTTACGGAGAAGGATAAAAGAGAGGCTGAGCTTCAAAGAAGGGTTGAGGAATCCAAGCAAAGAGAAGCATACTTGGAAAATGAACTTGCTAACATGTGGGTTCTTGTAGCAAAGTTGAAAAAAACTCATGGAGCTGAAACTGATGCTTCTGAATCAACAAGAGAGACCAGACTAACTGATGGCTTGGGGGGATGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005874 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032991 GO:0035371 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:1990752

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin

Protein Analysis

1008

Amino Acids

112.32

Weight (kDa)

6.32

Isoelectric Point (pI)

56.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	72 - 209	5.2e-19	Microtubule binding
Kinesin	PF00225	78 - 391	5.5e-106	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002243)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12020 AT3G12020 AT3G12020 AT3G12020 AT5G06670 AT5G06670 AT5G06670 AT5G06670
fragaria_vesca	FvH4_6g21780 FvH4_6g21780 FvH4_6g21780 FvH4_6g21780 FvH4_6g21780 FvH4_6g21780 FvH4_6g21780 FvH4_6g21780 FvH4_6g21780
malus_domestica	MD12G1054300.v1.1 MD14G1053100.v1.1
prunus_persica	Prupe.7G082400_v2.0.a1
pyrus_communis	pycom12g04730 pycom14g04410
rosa_chinensis	RchiOBHm_Chr3g0479141
rosa_laevigata	RLG00000023604
rosa_multiflora	Rmu_co8049936.1_g000001 Rmu_co8383491.1_g000001 Rmu_sc0002555.1_g000005
rosa_roxburghii	Rroxscaffold_6G00402700
rosa_rugosa	Rorug03G0171300 Rorug03G0171400
rosa_samantha	Rh3AG222600 Rh3BG255100 Rh3CG250700 Rh3DG247100 Rh6AG090700 Rh6BG153100 Rh6CG078500 Rh6DG074300 Rh6DG137500
rosa_wichuraiana	Rw0G001170 Rw1G014040 Rw3G019950 Rw6G024570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	85
Acc65I	GGTACC	2 cut(s)	671, 1882
AccB1I	GGYRCC	2 cut(s)	671, 1882
AccB7I	CCANNNNNTGG	1 cut(s)	2548
AccIII	TCCGGA	2 cut(s)	1979, 2715
AciI	CCGC	9 cut(s)	197, 232, 310, 468, 1071, 1552, 2411, 2670, 2825
AclWI	GGATC	5 cut(s)	25, 772, 942, 955, 2175
AcoI	YGGCCR	2 cut(s)	2667, 2790
AcsI	RAATTY	7 cut(s)	258, 643, 772, 1176, 1358, 1895, 2362
AcuI	CTGAAG	5 cut(s)	129, 1655, 2030, 2348, 2507
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	4 cut(s)	167, 439, 673, 1884
AfiI	CCNNNNNNNGG	5 cut(s)	468, 838, 1604, 2047, 2548
AflIII	ACRYGT	1 cut(s)	2922
AhlI	ACTAGT	2 cut(s)	1724, 1754
AjiI	CACGTC	1 cut(s)	2740
AjnI	CCWGG	4 cut(s)	505, 519, 1489, 1899
AjuI	GAANNNNNNNTTGG	4 cut(s)	86, 118, 2365, 2397
Alw21I	GWGCWC	6 cut(s)	131, 753, 911, 1838, 2008, 2604
Alw26I	GTCTC	9 cut(s)	291, 548, 1090, 1124, 1453, 1904, 2644, 2773, 2987
Alw44I	GTGCAC	1 cut(s)	2004
AlwI	GGATC	5 cut(s)	25, 772, 942, 955, 2175
AlwNI	CAGNNNCTG	2 cut(s)	2548, 2591
Aor13HI	TCCGGA	2 cut(s)	1979, 2715
AoxI	GGCC	4 cut(s)	358, 2667, 2762, 2790
ApaI	GGGCCC	1 cut(s)	2766
ApaLI	GTGCAC	1 cut(s)	2004
ApoI	RAATTY	7 cut(s)	258, 643, 772, 1176, 1358, 1895, 2362
ArsI	GACNNNNNNTTYG	4 cut(s)	201, 233, 2719, 2751
AseI	ATTAAT	1 cut(s)	957
Asp700I	GAANNNNTTC	2 cut(s)	1507, 2225
Asp718I	GGTACC	2 cut(s)	671, 1882
AspLEI	GCGC	1 cut(s)	2470
AspS9I	GGNCC	5 cut(s)	204, 229, 359, 2762, 2763
AsuC2I	CCSGG	1 cut(s)	1160
AsuHPI	GGTGA	5 cut(s)	451, 503, 866, 1475, 2145
AsuII	TTCGAA	2 cut(s)	92, 2229
AsuNHI	GCTAGC	1 cut(s)	1310
AvaII	GGWCC	2 cut(s)	204, 229
AxyI	CCTNAGG	1 cut(s)	1887
BaeGI	GKGCMC	2 cut(s)	2008, 2766
BaeI	ACNNNNGTAYC	2 cut(s)	663, 696
BalI	TGGCCA	1 cut(s)	2792
BamHI	GGATCC	1 cut(s)	947
BanI	GGYRCC	2 cut(s)	671, 1882
BanII	GRGCYC	5 cut(s)	131, 911, 1838, 2604, 2766
BbsI	GAAGAC	4 cut(s)	81, 479, 1510, 1655
Bbv12I	GWGCWC	6 cut(s)	131, 753, 911, 1838, 2008, 2604
BbvCI	CCTCAGC	1 cut(s)	2584
BccI	CCATC	9 cut(s)	287, 334, 998, 1346, 1477, 1771, 1849, 3002, 3015
BciT130I	CCWGG	4 cut(s)	507, 521, 1491, 1901
BclI	TGATCA	2 cut(s)	493, 1939
BcnI	CCSGG	1 cut(s)	1160
BcoDI	GTCTC	9 cut(s)	291, 548, 1090, 1124, 1453, 1904, 2644, 2773, 2987
BcuI	ACTAGT	2 cut(s)	1724, 1754
BfaI	CTAG	7 cut(s)	35, 977, 1311, 1725, 1755, 2276, 2654
BfmI	CTRYAG	2 cut(s)	1027, 1866
BfoI	RGCGCY	1 cut(s)	2471
BglII	AGATCT	2 cut(s)	597, 2286
BlpI	GCTNAGC	4 cut(s)	399, 1067, 2661, 2856
BmcAI	AGTACT	1 cut(s)	167
Bme1390I	CCNGG	5 cut(s)	507, 521, 1160, 1491, 1901
Bme18I	GGWCC	2 cut(s)	204, 229
BmgBI	CACGTC	1 cut(s)	2740
BmgT120I	GGNCC	5 cut(s)	204, 229, 359, 2762, 2763
BmiI	GGNNCC	7 cut(s)	40, 673, 797, 949, 1884, 2540, 2764
BmrFI	CCNGG	5 cut(s)	507, 521, 1160, 1491, 1901
BmtI	GCTAGC	1 cut(s)	1314
BpiI	GAAGAC	4 cut(s)	81, 479, 1510, 1655
BplI	GAGNNNNNCTC	2 cut(s)	1000, 1032
BpmI	CTGGAG	2 cut(s)	762, 2372
Bpu10I	CCTNAGC	2 cut(s)	666, 2584
Bpu1102I	GCTNAGC	4 cut(s)	399, 1067, 2661, 2856
Bpu14I	TTCGAA	2 cut(s)	92, 2229
BpuEI	CTTGAG	6 cut(s)	1622, 1758, 2255, 2380, 2492, 2588
BpuMI	CCSGG	1 cut(s)	1160
Bsa29I	ATCGAT	1 cut(s)	1947
BsaBI	GATNNNNATC	1 cut(s)	22
BsaHI	GRCGYC	1 cut(s)	5
BsaI	GGTCTC	2 cut(s)	1453, 2987
BsaJI	CCNNGG	4 cut(s)	251, 265, 519, 1900
BsaWI	WCCGGW	2 cut(s)	1979, 2715
BsaXI	ACNNNNNCTCC	2 cut(s)	1090, 1120
Bsc4I	CCNNNNNNNGG	5 cut(s)	468, 838, 1604, 2047, 2548
Bse1I	ACTGG	6 cut(s)	931, 1679, 1737, 2355, 2553, 2793
Bse21I	CCTNAGG	1 cut(s)	1887
Bse8I	GATNNNNATC	1 cut(s)	22
BseAI	TCCGGA	2 cut(s)	1979, 2715
BseBI	CCWGG	4 cut(s)	507, 521, 1491, 1901
BseCI	ATCGAT	1 cut(s)	1947
BseDI	CCNNGG	4 cut(s)	251, 265, 519, 1900
BseGI	GGATG	5 cut(s)	546, 667, 1009, 1576, 3026
BseJI	GATNNNNATC	1 cut(s)	22
BseLI	CCNNNNNNNGG	5 cut(s)	468, 838, 1604, 2047, 2548
BseNI	ACTGG	6 cut(s)	931, 1679, 1737, 2355, 2553, 2793
BseRI	GAGGAG	3 cut(s)	1538, 1587, 2039
BseSI	GKGCMC	2 cut(s)	2008, 2766
BseYI	CCCAGC	2 cut(s)	712, 2691
BsgI	GTGCAG	1 cut(s)	1081
Bsh1285I	CGRYCG	2 cut(s)	26, 349
BshFI	GGCC	4 cut(s)	360, 2669, 2764, 2792
BshNI	GGYRCC	2 cut(s)	671, 1882
BshVI	ATCGAT	1 cut(s)	1947
BsiEI	CGRYCG	2 cut(s)	26, 349
BsiHKAI	GWGCWC	6 cut(s)	131, 753, 911, 1838, 2008, 2604
BsiSI	CCGG	3 cut(s)	1159, 1980, 2716
BslFI	GGGAC	3 cut(s)	1568, 1723, 1917
BslI	CCNNNNNNNGG	5 cut(s)	468, 838, 1604, 2047, 2548
BsmAI	GTCTC	9 cut(s)	291, 548, 1090, 1124, 1453, 1904, 2644, 2773, 2987
BsmBI	CGTCTC	2 cut(s)	1124, 2773
BsmFI	GGGAC	3 cut(s)	1568, 1723, 1917
BsmI	GAATGC	2 cut(s)	2300, 2425
BsnI	GGCC	4 cut(s)	360, 2669, 2764, 2792
Bso31I	GGTCTC	2 cut(s)	1453, 2987
Bsp119I	TTCGAA	2 cut(s)	92, 2229
Bsp120I	GGGCCC	1 cut(s)	2762
Bsp1286I	GDGCHC	7 cut(s)	131, 753, 911, 1838, 2008, 2604, 2766
Bsp13I	TCCGGA	2 cut(s)	1979, 2715
Bsp1720I	GCTNAGC	4 cut(s)	399, 1067, 2661, 2856
BspACI	CCGC	9 cut(s)	197, 232, 310, 468, 1071, 1552, 2411, 2670, 2825
BspANI	GGCC	4 cut(s)	360, 2669, 2764, 2792
BspDI	ATCGAT	1 cut(s)	1947
BspEI	TCCGGA	2 cut(s)	1979, 2715
BspHI	TCATGA	1 cut(s)	2634
BspLI	GGNNCC	7 cut(s)	40, 673, 797, 949, 1884, 2540, 2764
BspOI	GCTAGC	1 cut(s)	1314
BspPI	GGATC	5 cut(s)	25, 772, 942, 955, 2175
BspQI	GCTCTTC	1 cut(s)	741
BspT104I	TTCGAA	2 cut(s)	92, 2229
BspT107I	GGYRCC	2 cut(s)	671, 1882
BspTNI	GGTCTC	2 cut(s)	1453, 2987
BsrI	ACTGG	6 cut(s)	931, 1679, 1737, 2355, 2553, 2793
BssECI	CCNNGG	4 cut(s)	251, 265, 519, 1900
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	2 cut(s)	251, 265
Bst2UI	CCWGG	4 cut(s)	507, 521, 1491, 1901
Bst6I	CTCTTC	6 cut(s)	741, 1122, 1251, 1515, 2722, 2774
BstACI	GRCGYC	1 cut(s)	5
BstAPI	GCANNNNNTGC	1 cut(s)	287
BstBI	TTCGAA	2 cut(s)	92, 2229
BstC8I	GCNNGC	5 cut(s)	1312, 1530, 2409, 2466, 2667
BstEII	GGTNACC	1 cut(s)	457
BstF5I	GGATG	5 cut(s)	546, 667, 1009, 1576, 3026
BstH2I	RGCGCY	1 cut(s)	2471
BstHHI	GCGC	1 cut(s)	2470
BstMAI	GTCTC	9 cut(s)	291, 548, 1090, 1124, 1453, 1904, 2644, 2773, 2987
BstMCI	CGRYCG	2 cut(s)	26, 349
BstMWI	GCNNNNNNNGC	6 cut(s)	287, 316, 1160, 1403, 2588, 2666
BstNI	CCWGG	4 cut(s)	507, 521, 1491, 1901
BstNSI	RCATGY	4 cut(s)	407, 761, 2411, 2926
BstPI	GGTNACC	1 cut(s)	457
BstSCI	CCNGG	5 cut(s)	505, 519, 1158, 1489, 1899
BstSFI	CTRYAG	2 cut(s)	1027, 1866
BstSLI	GKGCMC	2 cut(s)	2008, 2766
BstV2I	GAAGAC	4 cut(s)	81, 479, 1510, 1655
BstX2I	RGATCY	3 cut(s)	597, 947, 2286
BstXI	CCANNNNNNTGG	1 cut(s)	2164
BstYI	RGATCY	3 cut(s)	597, 947, 2286
Bsu15I	ATCGAT	1 cut(s)	1947
Bsu36I	CCTNAGG	1 cut(s)	1887
BsuRI	GGCC	4 cut(s)	360, 2669, 2764, 2792
BsuTUI	ATCGAT	1 cut(s)	1947
BtrI	CACGTC	1 cut(s)	2740
BtsCI	GGATG	5 cut(s)	546, 667, 1009, 1576, 3026
BtsI	GCAGTG	1 cut(s)	2430
BtsIMutI	CAGTG	9 cut(s)	228, 812, 1098, 1631, 1744, 2006, 2348, 2430, 2764
Cac8I	GCNNGC	5 cut(s)	1312, 1530, 2409, 2466, 2667
CaiI	CAGNNNCTG	2 cut(s)	2548, 2591
CciI	TCATGA	1 cut(s)	2634
CfoI	GCGC	1 cut(s)	2470
Cfr13I	GGNCC	5 cut(s)	204, 229, 359, 2762, 2763
ClaI	ATCGAT	1 cut(s)	1947
CpoI	CGGWCCG	1 cut(s)	229
CseI	GACGC	2 cut(s)	1141, 2684
CsiI	ACCWGGT	1 cut(s)	1489
Csp6I	GTAC	4 cut(s)	166, 438, 672, 1883
CspI	CGGWCCG	1 cut(s)	229
CviQI	GTAC	4 cut(s)	166, 438, 672, 1883
DraI	TTTAAA	1 cut(s)	1251
DrdI	GACNNNNNNGTC	1 cut(s)	85
DseDI	GACNNNNNNGTC	1 cut(s)	85
EaeI	YGGCCR	2 cut(s)	2667, 2790
Eam1104I	CTCTTC	6 cut(s)	741, 1122, 1251, 1515, 2722, 2774
EarI	CTCTTC	6 cut(s)	741, 1122, 1251, 1515, 2722, 2774
Ecl136II	GAGCTC	4 cut(s)	129, 909, 1836, 2602
Eco130I	CCWWGG	2 cut(s)	251, 265
Eco24I	GRGCYC	5 cut(s)	131, 911, 1838, 2604, 2766
Eco31I	GGTCTC	2 cut(s)	1453, 2987
Eco47I	GGWCC	2 cut(s)	204, 229
Eco53kI	GAGCTC	4 cut(s)	129, 909, 1836, 2602
Eco57I	CTGAAG	5 cut(s)	129, 1655, 2030, 2348, 2507
Eco81I	CCTNAGG	1 cut(s)	1887
Eco91I	GGTNACC	1 cut(s)	457
EcoICRI	GAGCTC	4 cut(s)	129, 909, 1836, 2602
EcoO65I	GGTNACC	1 cut(s)	457
EcoRII	CCWGG	4 cut(s)	505, 519, 1489, 1899
EcoT14I	CCWWGG	2 cut(s)	251, 265
EcoT22I	ATGCAT	2 cut(s)	343, 489
EcoT38I	GRGCYC	5 cut(s)	131, 911, 1838, 2604, 2766
ErhI	CCWWGG	2 cut(s)	251, 265
Esp3I	CGTCTC	2 cut(s)	1124, 2773
FalI	AAGNNNNNCTT	4 cut(s)	1512, 1544, 2436, 2468
FaqI	GGGAC	3 cut(s)	1568, 1723, 1917
FauI	CCCGC	3 cut(s)	461, 1545, 2404
FauNDI	CATATG	3 cut(s)	451, 2566, 2818
FbaI	TGATCA	2 cut(s)	493, 1939
FokI	GGATG	4 cut(s)	533, 674, 1016, 1583
FriOI	GRGCYC	5 cut(s)	131, 911, 1838, 2604, 2766
FspBI	CTAG	7 cut(s)	35, 977, 1311, 1725, 1755, 2276, 2654
GlaI	GCGC	1 cut(s)	2469
GsaI	CCCAGC	2 cut(s)	716, 2695
GsuI	CTGGAG	2 cut(s)	762, 2372
HaeII	RGCGCY	1 cut(s)	2471
HaeIII	GGCC	4 cut(s)	360, 2669, 2764, 2792
HapII	CCGG	3 cut(s)	1159, 1980, 2716
HgaI	GACGC	2 cut(s)	1141, 2684
HhaI	GCGC	1 cut(s)	2470
Hin1I	GRCGYC	1 cut(s)	5
Hin6I	GCGC	1 cut(s)	2468
HinP1I	GCGC	1 cut(s)	2468
HincII	GTYRAC	4 cut(s)	433, 999, 1431, 1760
HindII	GTYRAC	4 cut(s)	433, 999, 1431, 1760
HpaII	CCGG	3 cut(s)	1159, 1980, 2716
HphI	GGTGA	5 cut(s)	451, 503, 866, 1475, 2145
Hpy166II	GTNNAC	6 cut(s)	242, 433, 999, 1431, 1760, 2006
Hpy8I	GTNNAC	6 cut(s)	242, 433, 999, 1431, 1760, 2006
Hpy99I	CGWCG	1 cut(s)	2700
HpyCH4IV	ACGT	1 cut(s)	2739
HpyF10VI	GCNNNNNNNGC	6 cut(s)	287, 316, 1160, 1403, 2588, 2666
HpySE526I	ACGT	1 cut(s)	2739
Hsp92I	GRCGYC	1 cut(s)	5
HspAI	GCGC	1 cut(s)	2468
Kpn2I	TCCGGA	2 cut(s)	1979, 2715
KpnI	GGTACC	2 cut(s)	675, 1886
Ksp22I	TGATCA	2 cut(s)	493, 1939
LguI	GCTCTTC	1 cut(s)	741
LmnI	GCTCC	3 cut(s)	795, 1525, 2960
MabI	ACCWGGT	1 cut(s)	1489
MaeI	CTAG	7 cut(s)	35, 977, 1311, 1725, 1755, 2276, 2654
MaeII	ACGT	1 cut(s)	2739
MaeIII	GTNAC	9 cut(s)	217, 223, 457, 726, 1102, 1212, 1285, 2705, 2735
MfeI	CAATTG	3 cut(s)	875, 1935, 2210
MflI	RGATCY	3 cut(s)	597, 947, 2286
MhlI	GDGCHC	7 cut(s)	131, 753, 911, 1838, 2008, 2604, 2766
MlsI	TGGCCA	1 cut(s)	2792
MluNI	TGGCCA	1 cut(s)	2792
MlyI	GAGTC	9 cut(s)	438, 469, 550, 973, 1645, 2377, 2387, 2522, 2743
MmeI	TCCRAC	1 cut(s)	742
Mox20I	TGGCCA	1 cut(s)	2792
Mph1103I	ATGCAT	2 cut(s)	343, 489
MroI	TCCGGA	2 cut(s)	1979, 2715
MroXI	GAANNNNTTC	2 cut(s)	1507, 2225
MscI	TGGCCA	1 cut(s)	2792
MslI	CAYNNNNRTG	6 cut(s)	384, 756, 1080, 2001, 2162, 2439
Msp20I	TGGCCA	1 cut(s)	2792
MspA1I	CMGCKG	3 cut(s)	468, 740, 2588
MspI	CCGG	3 cut(s)	1159, 1980, 2716
MspR9I	CCNGG	5 cut(s)	507, 521, 1160, 1491, 1901
MunI	CAATTG	3 cut(s)	875, 1935, 2210
Mva1269I	GAATGC	2 cut(s)	2300, 2425
MvaI	CCWGG	4 cut(s)	507, 521, 1491, 1901
MwoI	GCNNNNNNNGC	6 cut(s)	287, 316, 1160, 1403, 2588, 2666
NciI	CCSGG	1 cut(s)	1160
NdeI	CATATG	3 cut(s)	451, 2566, 2818
NheI	GCTAGC	1 cut(s)	1310
NlaIV	GGNNCC	7 cut(s)	40, 673, 797, 949, 1884, 2540, 2764
NmuCI	GTSAC	6 cut(s)	217, 223, 457, 726, 1212, 2735
NsiI	ATGCAT	2 cut(s)	343, 489
NspI	RCATGY	4 cut(s)	407, 761, 2411, 2926
NspV	TTCGAA	2 cut(s)	92, 2229
PaeI	GCATGC	1 cut(s)	2411
PagI	TCATGA	1 cut(s)	2634
PciI	ACATGT	1 cut(s)	2922
PciSI	GCTCTTC	1 cut(s)	741
PcsI	WCGNNNNNNNCGW	1 cut(s)	83
PctI	GAATGC	2 cut(s)	2300, 2425
PdmI	GAANNNNTTC	2 cut(s)	1507, 2225
PflFI	GACNNNGTC	2 cut(s)	1491, 2512
PflMI	CCANNNNNTGG	1 cut(s)	2548
Ple19I	CGATCG	2 cut(s)	26, 349
PleI	GAGTC	9 cut(s)	437, 469, 550, 972, 1645, 2376, 2387, 2521, 2742
PpsI	GAGTC	9 cut(s)	437, 469, 550, 972, 1645, 2376, 2387, 2521, 2742
PscI	ACATGT	1 cut(s)	2922
PshBI	ATTAAT	1 cut(s)	957
Psp124BI	GAGCTC	4 cut(s)	131, 911, 1838, 2604
Psp6I	CCWGG	4 cut(s)	505, 519, 1489, 1899
PspEI	GGTNACC	1 cut(s)	457
PspFI	CCCAGC	2 cut(s)	712, 2691
PspGI	CCWGG	4 cut(s)	505, 519, 1489, 1899
PspN4I	GGNNCC	7 cut(s)	40, 673, 797, 949, 1884, 2540, 2764
PspOMI	GGGCCC	1 cut(s)	2762
PspPI	GGNCC	5 cut(s)	204, 229, 359, 2762, 2763
PstNI	CAGNNNCTG	2 cut(s)	2548, 2591
PsuI	RGATCY	3 cut(s)	597, 947, 2286
PsyI	GACNNNGTC	2 cut(s)	1491, 2512
PvuI	CGATCG	2 cut(s)	26, 349
PvuII	CAGCTG	2 cut(s)	740, 2588
RsaI	GTAC	4 cut(s)	167, 439, 673, 1884
RsaNI	GTAC	4 cut(s)	166, 438, 672, 1883
RseI	CAYNNNNRTG	6 cut(s)	384, 756, 1080, 2001, 2162, 2439
Rsr2I	CGGWCCG	1 cut(s)	229
RsrII	CGGWCCG	1 cut(s)	229
SacI	GAGCTC	4 cut(s)	131, 911, 1838, 2604
SapI	GCTCTTC	1 cut(s)	741
Sau96I	GGNCC	5 cut(s)	204, 229, 359, 2762, 2763
ScaI	AGTACT	1 cut(s)	167
SchI	GAGTC	9 cut(s)	438, 469, 550, 973, 1645, 2377, 2387, 2522, 2743
ScrFI	CCNGG	5 cut(s)	507, 521, 1160, 1491, 1901
SduI	GDGCHC	7 cut(s)	131, 753, 911, 1838, 2008, 2604, 2766
SexAI	ACCWGGT	1 cut(s)	1489
SfcI	CTRYAG	2 cut(s)	1027, 1866
SfuI	TTCGAA	2 cut(s)	92, 2229
SinI	GGWCC	2 cut(s)	204, 229
SmiMI	CAYNNNNRTG	6 cut(s)	384, 756, 1080, 2001, 2162, 2439
SmlI	CTYRAG	6 cut(s)	1601, 1773, 2234, 2395, 2471, 2603
SmoI	CTYRAG	6 cut(s)	1601, 1773, 2234, 2395, 2471, 2603
SpeI	ACTAGT	2 cut(s)	1724, 1754
SphI	GCATGC	1 cut(s)	2411
SsiI	CCGC	9 cut(s)	197, 232, 310, 468, 1071, 1552, 2411, 2670, 2825
SspI	AATATT	1 cut(s)	101
SspMI	CTAG	7 cut(s)	35, 977, 1311, 1725, 1755, 2276, 2654
SstI	GAGCTC	4 cut(s)	131, 911, 1838, 2604
StyD4I	CCNGG	5 cut(s)	505, 519, 1158, 1489, 1899
StyI	CCWWGG	2 cut(s)	251, 265
TaiI	ACGT	1 cut(s)	2742
TatI	WGTACW	1 cut(s)	165
TauI	GCSGC	2 cut(s)	2672, 2828
TscAI	CASTG	9 cut(s)	228, 819, 1105, 1638, 1744, 2013, 2355, 2430, 2764
TseFI	GTSAC	6 cut(s)	217, 223, 457, 726, 1212, 2735
Tsp45I	GTSAC	6 cut(s)	217, 223, 457, 726, 1212, 2735
TspGWI	ACGGA	5 cut(s)	57, 191, 1016, 1702, 2849
TspRI	CASTG	9 cut(s)	228, 819, 1105, 1638, 1744, 2013, 2355, 2430, 2764
Tth111I	GACNNNGTC	2 cut(s)	1491, 2512
Van91I	CCANNNNNTGG	1 cut(s)	2548
VneI	GTGCAC	1 cut(s)	2004
VpaK11BI	GGWCC	2 cut(s)	204, 229
VspI	ATTAAT	1 cut(s)	957
XapI	RAATTY	7 cut(s)	258, 643, 772, 1176, 1358, 1895, 2362
XceI	RCATGY	4 cut(s)	407, 761, 2411, 2926
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2699
XmnI	GAANNNNTTC	2 cut(s)	1507, 2225
XspI	CTAG	7 cut(s)	35, 977, 1311, 1725, 1755, 2276, 2654
ZrmI	AGTACT	1 cut(s)	167
Zsp2I	ATGCAT	2 cut(s)	343, 489

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.