Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0002243

Physical Location & Seq

Genomic Coordinates Forward (+)

6117055 .. 6133617

Gene Structure 16563 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1054300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3024 bp

ATGGCGTCAAAATACGGATCAAAATCAAAAAAATTAGGTTCGACCAGTTCAAAGGCTGCTAATTCCCCTGCATCTTCGACTACAACATCTTCAAAGCAATATTTGGAAACATCTATTGAATGTCAGAGCTCTCCAGCATCTTCTTCAGCTCGAAGTAAGCAAAGTAAGCAACAGTACTTGTACTCGGAAAGTTTGCCGCAAGATGGGGAAAAATCCAAAGAAAACGTCACTGTGACGGTCCGCTTTCGTCCACTCAGCCCGAGGGAAATTCGCCAAGGAGAAGAAATCGCATGGTATGCAGATGGAGACACCATTGTGCGAAATGAGCATAATCCATCAATAGCTTATGCATATGATTGGGTTTTCGGCCCTACAACAACAACGCGCCATGTCTATGATGTTGCTGCTCAGCATGTTATTAGTGGCGCTATGGAAGGCGTCAACGGTACAATTTTTGCATATGGGGTGACCAGCAGCGGGAAGACTCACACTATGCATGGTGATCAAAGGTCGCCTGGAATTATACCACTGGCTGTGAAGGATGCTTTTAGCATCATCCAAGAGACTCCAAATCGAGAGTATCTTCTTCGTGTGTCATACTTGGAAATCTATAATGAGGTTGTTAATGACTTGTTGAATCCAGCAGGACAGAATTTACGAATTAGAGAAGATGCTCAGGGCACTTTTGTTGAAGGAATAAAGGAAGAAGTTGTACTATCCCCAGCTCACGCCCTTTCACTTATAGCAGCTGGAGAAGAGCACAGGCATGTTGGTTCGACAAATTTCAATCTACTCAGTAGCAGGAGCCACACGATATTTTCGCTGACAATAGAGAGTAGTCCATGTGGGGAAAATTGTGAAGGTGAAGCTGTAAGTCTGTCGCAATTGAACCTCATCGATCTGGCAGGTTCTGAGAGCTCGAAAGCTGAAACTACTGGTGTCAGACGGAAAGAAGGATCCTATATTAATAAAAGTCTGCTAACTCTAGGAACTGTTATATCAAAGTTGACAGATACGAAGGCTACTCATATACCATACAGGGATTCTAAATTGACAAGACTTCTTCAATCTTCTCTAAGTGGACATGGGCGTGTATCTCTCATCTGCACTGTTACTCCTTCATCAAGCAGTTCCGAAGAGACACATAATACATTGAAATTTGCACATCGGGCAAAACACATTGAAATTCAAGCAGCACAAAACAAGATTATTGATGAGAAGTCACTCATTAAGAAATATCAAAACGAGATACGTAGTTTAAAGGAAGAGTTAGAACAGTTGAAGAGGGGGATAGTCACAATTCCTCAACTAAAAGATGCTGGGGAAGATGATATTCTGCTCCTTAAACAAAAGTTAGAAGATGGCAAATACAAACTGCAATCAAGACTGGAACAAGAAGAAGAAGCCAAAGCTGCTTTGTTGGGAAGAATCCAAAGGTTGACAAAATTGATTTTGGTCTCAACAAAAGAAACACAGCCGTCAAGGTTTTCTCACCGACCTGGTGTTAGAAGAAGACATTCCTTTGGTGAAGAGGAGCTTGCATACCTACCATACAAAAGGCGGGACTTGATTTTGGATGATGAAAGCATTGAATTGTTTGTTCCTCCTCTTGAGGGGAGTACTGAAACGACTGATGACATACTGAAGGGGGAGAAGAAGACTCGGAAGCATGGACTTTTAAACTGGTTAAAAATACGGAAACGTGATGGTGGTACGGGGACCTTGACAAGCACAAGTGATAAATCCAGTGGAATAACTACTAGTGCACCTTCAACACCTCAAGCGGAAAGTGGTAATTTTCATGCAGAATCCAGACTTTCACATTCAGTGCTTACAGAAAGCTTTCCATCGGCTGAGTCCGAGGCTAGAGAGGACAGAGAAGTTCTTCAGGAAAATTTCCTGGGACGAGAGACACCTCTGACTAGCATGAAGTCAATTGATCAGATCGATCTTTTAAGGGAGCAGCAAAAAATCTTGTCTGATGAGGTAGCACTCCATTTAAGTGCTCTGAAGCGGTTGTCTGAGGAGGCTTCAAGACACTCTGAAAAGGATGAAATTAAGATGGAAATGCAAAAGTTAAAAGATGAAATCAAGGCGAAGAAGGAACAAATAGCTTTGGTGGAAAAGAAAATTGGAGAGTCCTTTTCACACAACAAAATGGGTCAACTGGAAGCATCCCAATCTATTGCTGAAGTGATGGAGCAATTGAATGAGAAGTCCTTCGAACTTGAGGTTAAAGCTGCTGATAATCGCATAATCCAAGAACAGCTAGAACAAAAGATCGGGGAATGCAAAGGATTGCAGGAGACAATCGCCTCCTTGAAACAGCAGCTTTCTGAGGCACTAGAGTTCAAAAATTTGAGCCCGATAGTCACTTCTCGAACTCGTTCCAAAAACTTGGATGAGGAACTGTGTACAGAGAAAGATAATGCAGCGTTGGATGACAAAAATGAAGTTTTTCTTCTACGGAAGCAGGTCGAAGAACTGAAGCAGAAGGTGGTTGAACTGACGGAGTCAAAGGAACAGTTAGAAGTTCGGAACCAGAAACTTGTGGAGGAGAGCTCGTATGCCAAAGGTCTAGCCTCAGCTGCTGCCGTTGAGCTCAAGGCATTATCAGAAGAAGTTTCCAAACTCATGAACCAAAACGAAAAACTCACTGCTGAGCTGGCCGCATCCAAGAACTCTCCTGCACAGCGTCGAAATAGTAGCACACTCCGAAATGGTCGGAGAGAGAGTCACACCAAACAGGACCAAAGTGGGCTTGTCTCAGAAATGAAGAGAGAACTGGCGGTGAGTAAGGAGAGAGAGCATTCATACGAGGCTGCTCTTACGGAAAAGGATAAAAGAGAGGCTGAGCTTCATAGAAGGGTTGAGGAATCCAAGCAAAGAGAAGCGTACTTGGAAAATGAACTCGCCAACATGTGGGTTCTGGTGGCAAAGTTAAAAAAATCGCACGGAACTGAAACTGATTCTTCGGACTCAACGAAAGAGACCCGACAAACTGATGGTTATGCGGGATGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005874 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032991 GO:0035371 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:1990752

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin

Protein Analysis

1008

Amino Acids

112.57

Weight (kDa)

6.25

Isoelectric Point (pI)

54.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	75 - 212	6.6e-18	Microtubule binding
Kinesin	PF00225	81 - 394	4e-105	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002243)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12020 AT3G12020 AT3G12020 AT3G12020 AT5G06670 AT5G06670 AT5G06670 AT5G06670
fragaria_vesca	FvH4_6g21780 FvH4_6g21780 FvH4_6g21780 FvH4_6g21780 FvH4_6g21780 FvH4_6g21780 FvH4_6g21780 FvH4_6g21780 FvH4_6g21780
malus_domestica	MD12G1054300.v1.1 MD14G1053100.v1.1
prunus_persica	Prupe.7G082400_v2.0.a1
pyrus_communis	pycom12g04730 pycom14g04410
rosa_chinensis	RchiOBHm_Chr3g0479141
rosa_laevigata	RLG00000023604
rosa_multiflora	Rmu_co8049936.1_g000001 Rmu_co8383491.1_g000001 Rmu_sc0002555.1_g000005
rosa_roxburghii	Rroxscaffold_6G00402700
rosa_rugosa	Rorug03G0171300 Rorug03G0171400
rosa_samantha	Rh3AG222600 Rh3BG255100 Rh3CG250700 Rh3DG247100 Rh6AG090700 Rh6BG153100 Rh6CG078500 Rh6DG074300 Rh6DG137500
rosa_wichuraiana	Rw0G001170 Rw1G014040 Rw3G019950 Rw6G024570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	896, 2464
AccB7I	CCANNNNNTGG	1 cut(s)	2922
AccII	CGCG	1 cut(s)	385
AciI	CCGC	9 cut(s)	197, 241, 477, 1561, 1784, 2014, 2670, 2789, 3014
AclWI	GGATC	3 cut(s)	25, 951, 964
AcoI	YGGCCR	1 cut(s)	2667
AcsI	RAATTY	7 cut(s)	267, 652, 781, 1157, 1185, 1894, 2356
AcuI	CTGAAG	6 cut(s)	129, 1664, 1871, 2030, 2211, 2507
AcyI	GRCGYC	2 cut(s)	5, 438
AfaI	GTAC	8 cut(s)	176, 182, 448, 714, 1621, 1714, 2416, 2897
AfiI	CCNNNNNNNGG	4 cut(s)	203, 477, 1613, 2922
AflIII	ACRYGT	1 cut(s)	2919
AhlI	ACTAGT	1 cut(s)	1760
AjnI	CCWGG	3 cut(s)	514, 1498, 1899
AjuI	GAANNNNNNNTTGG	2 cut(s)	2115, 2147
AleI	CACNNNNGTG	1 cut(s)	314
Alw21I	GWGCWC	7 cut(s)	131, 762, 920, 1768, 2008, 2564, 2604
Alw26I	GTCTC	8 cut(s)	300, 557, 1133, 1462, 1904, 2300, 2770, 2984
Alw44I	GTGCAC	1 cut(s)	1764
AlwI	GGATC	3 cut(s)	25, 951, 964
AlwNI	CAGNNNCTG	2 cut(s)	911, 2591
Ama87I	CYCGRG	1 cut(s)	259
AoxI	GGCC	2 cut(s)	367, 2667
ApaLI	GTGCAC	1 cut(s)	1764
ApoI	RAATTY	7 cut(s)	267, 652, 781, 1157, 1185, 1894, 2356
ArsI	GACNNNNNNTTYG	2 cut(s)	210, 242
AseI	ATTAAT	1 cut(s)	966
Asp700I	GAANNNNTTC	6 cut(s)	1516, 1842, 1884, 2219, 2386, 2457
AspLEI	GCGC	2 cut(s)	387, 428
AspS9I	GGNCC	4 cut(s)	238, 368, 1719, 2749
AsuHPI	GGTGA	6 cut(s)	478, 512, 875, 1484, 1538, 2803
AsuII	TTCGAA	1 cut(s)	2223
AvaI	CYCGRG	1 cut(s)	259
AvaII	GGWCC	3 cut(s)	238, 1719, 2749
BaeGI	GKGCMC	2 cut(s)	683, 1768
BamHI	GGATCC	1 cut(s)	956
BanII	GRGCYC	5 cut(s)	131, 920, 2366, 2564, 2604
BarI	GAAGNNNNNNTAC	2 cut(s)	696, 728
BbsI	GAAGAC	3 cut(s)	488, 1519, 1664
Bbv12I	GWGCWC	7 cut(s)	131, 762, 920, 1768, 2008, 2564, 2604
BbvCI	CCTCAGC	1 cut(s)	2584
BceAI	ACGGC	2 cut(s)	1462, 2579
BciT130I	CCWGG	3 cut(s)	516, 1500, 1901
BclI	TGATCA	2 cut(s)	502, 1939
BcoDI	GTCTC	8 cut(s)	300, 557, 1133, 1462, 1904, 2300, 2770, 2984
BcuI	ACTAGT	1 cut(s)	1760
BfaI	CTAG	7 cut(s)	986, 1761, 1866, 1923, 2270, 2345, 2579
BfoI	RGCGCY	1 cut(s)	429
BfuAI	ACCTGC	2 cut(s)	896, 2464
BlpI	GCTNAGC	3 cut(s)	408, 2661, 2853
BmcAI	AGTACT	2 cut(s)	176, 1621
Bme1390I	CCNGG	3 cut(s)	516, 1500, 1901
Bme18I	GGWCC	3 cut(s)	238, 1719, 2749
BmeT110I	CYCGRG	1 cut(s)	259
BmgT120I	GGNCC	4 cut(s)	238, 368, 1719, 2749
BmiI	GGNNCC	4 cut(s)	806, 958, 1720, 2540
BmrFI	CCNGG	3 cut(s)	516, 1500, 1901
BmsI	GCATC	8 cut(s)	80, 146, 532, 561, 661, 1306, 2183, 2681
BpiI	GAAGAC	3 cut(s)	488, 1519, 1664
BplI	GAGNNNNNCTC	2 cut(s)	2546, 2578
BpmI	CTGGAG	2 cut(s)	117, 771
Bpu10I	CCTNAGC	2 cut(s)	675, 2584
Bpu1102I	GCTNAGC	3 cut(s)	408, 2661, 2853
Bpu14I	TTCGAA	1 cut(s)	2223
BpuEI	CTTGAG	4 cut(s)	1631, 1764, 2249, 2588
Bsa29I	ATCGAT	2 cut(s)	897, 1947
BsaAI	YACGTR	1 cut(s)	1253
BsaBI	GATNNNNATC	1 cut(s)	22
BsaHI	GRCGYC	2 cut(s)	5, 438
BsaI	GGTCTC	2 cut(s)	1462, 2984
BsaJI	CCNNGG	4 cut(s)	260, 274, 1860, 1900
BsaXI	ACNNNNNCTCC	6 cut(s)	1099, 1129, 2549, 2579, 2719, 2749
Bsc4I	CCNNNNNNNGG	4 cut(s)	203, 477, 1613, 2922
Bse1I	ACTGG	8 cut(s)	45, 534, 940, 1392, 1688, 1746, 2172, 2790
Bse8I	GATNNNNATC	1 cut(s)	22
BseBI	CCWGG	3 cut(s)	516, 1500, 1901
BseCI	ATCGAT	2 cut(s)	897, 1947
BseDI	CCNNGG	4 cut(s)	260, 274, 1860, 1900
BseGI	GGATG	9 cut(s)	547, 555, 1582, 2056, 2174, 2407, 2446, 2672, 3023
BseJI	GATNNNNATC	1 cut(s)	22
BseLI	CCNNNNNNNGG	4 cut(s)	203, 477, 1613, 2922
BseNI	ACTGG	8 cut(s)	45, 534, 940, 1392, 1688, 1746, 2172, 2790
BseRI	GAGGAG	4 cut(s)	1547, 1596, 2039, 2570
BseSI	GKGCMC	2 cut(s)	683, 1768
BseYI	CCCAGC	2 cut(s)	721, 1319
BsgI	GTGCAG	2 cut(s)	1090, 2673
Bsh1236I	CGCG	1 cut(s)	385
BshFI	GGCC	2 cut(s)	369, 2669
BshVI	ATCGAT	2 cut(s)	897, 1947
BsiHKAI	GWGCWC	7 cut(s)	131, 762, 920, 1768, 2008, 2564, 2604
BsiHKCI	CYCGRG	1 cut(s)	259
BslFI	GGGAC	3 cut(s)	1577, 1732, 1917
BslI	CCNNNNNNNGG	4 cut(s)	203, 477, 1613, 2922
BsmAI	GTCTC	8 cut(s)	300, 557, 1133, 1462, 1904, 2300, 2770, 2984
BsmFI	GGGAC	3 cut(s)	1577, 1732, 1917
BsmI	GAATGC	2 cut(s)	2294, 2809
BsnI	GGCC	2 cut(s)	369, 2669
Bso31I	GGTCTC	2 cut(s)	1462, 2984
BsoBI	CYCGRG	1 cut(s)	259
Bsp119I	TTCGAA	1 cut(s)	2223
Bsp1286I	GDGCHC	9 cut(s)	131, 683, 762, 920, 1768, 2008, 2366, 2564, 2604
Bsp1407I	TGTACA	1 cut(s)	2414
Bsp143I	GATC	8 cut(s)	17, 502, 898, 956, 1939, 1944, 1948, 2280
Bsp1720I	GCTNAGC	3 cut(s)	408, 2661, 2853
BspACI	CCGC	9 cut(s)	197, 241, 477, 1561, 1784, 2014, 2670, 2789, 3014
BspANI	GGCC	2 cut(s)	369, 2669
BspDI	ATCGAT	2 cut(s)	897, 1947
BspFNI	CGCG	1 cut(s)	385
BspHI	TCATGA	1 cut(s)	2634
BspLI	GGNNCC	4 cut(s)	806, 958, 1720, 2540
BspMI	ACCTGC	2 cut(s)	896, 2464
BspPI	GGATC	3 cut(s)	25, 951, 964
BspQI	GCTCTTC	1 cut(s)	750
BspT104I	TTCGAA	1 cut(s)	2223
BspTNI	GGTCTC	2 cut(s)	1462, 2984
BsrGI	TGTACA	1 cut(s)	2414
BsrI	ACTGG	8 cut(s)	45, 534, 940, 1392, 1688, 1746, 2172, 2790
BssECI	CCNNGG	4 cut(s)	260, 274, 1860, 1900
BssMI	GATC	8 cut(s)	17, 502, 898, 956, 1939, 1944, 1948, 2280
BssNI	GRCGYC	2 cut(s)	5, 438
BssT1I	CCWWGG	1 cut(s)	274
Bst2UI	CCWGG	3 cut(s)	516, 1500, 1901
Bst4CI	ACNGT	9 cut(s)	174, 232, 238, 446, 994, 1111, 1278, 2412, 2526
Bst6I	CTCTTC	6 cut(s)	750, 1131, 1260, 1277, 1524, 2771
BstACI	GRCGYC	2 cut(s)	5, 438
BstAPI	GCANNNNNTGC	1 cut(s)	296
BstAUI	TGTACA	1 cut(s)	2414
BstBAI	YACGTR	1 cut(s)	1253
BstBI	TTCGAA	1 cut(s)	2223
BstC8I	GCNNGC	2 cut(s)	1539, 2667
BstEII	GGTNACC	1 cut(s)	466
BstF5I	GGATG	9 cut(s)	547, 555, 1582, 2056, 2174, 2407, 2446, 2672, 3023
BstFNI	CGCG	1 cut(s)	385
BstH2I	RGCGCY	1 cut(s)	429
BstHHI	GCGC	2 cut(s)	387, 428
BstKTI	GATC	8 cut(s)	20, 505, 901, 959, 1942, 1947, 1951, 2283
BstMAI	GTCTC	8 cut(s)	300, 557, 1133, 1462, 1904, 2300, 2770, 2984
BstMBI	GATC	8 cut(s)	17, 502, 898, 956, 1939, 1944, 1948, 2280
BstMWI	GCNNNNNNNGC	7 cut(s)	166, 296, 325, 1169, 1412, 2588, 2666
BstNI	CCWGG	3 cut(s)	516, 1500, 1901
BstNSI	RCATGY	3 cut(s)	416, 770, 2923
BstPI	GGTNACC	1 cut(s)	466
BstSCI	CCNGG	3 cut(s)	514, 1498, 1899
BstSLI	GKGCMC	2 cut(s)	683, 1768
BstSNI	TACGTA	1 cut(s)	1253
BstUI	CGCG	1 cut(s)	385
BstV2I	GAAGAC	3 cut(s)	488, 1519, 1664
BstX2I	RGATCY	1 cut(s)	956
BstXI	CCANNNNNNTGG	1 cut(s)	2398
BstYI	RGATCY	1 cut(s)	956
Bsu15I	ATCGAT	2 cut(s)	897, 1947
BsuRI	GGCC	2 cut(s)	369, 2669
BsuTUI	ATCGAT	2 cut(s)	897, 1947
BtsCI	GGATG	9 cut(s)	547, 555, 1582, 2056, 2174, 2407, 2446, 2672, 3023
BtsI	GCAGTG	1 cut(s)	2655
BtsIMutI	CAGTG	6 cut(s)	228, 527, 1107, 1753, 1833, 2655
BveI	ACCTGC	2 cut(s)	896, 2464
Cac8I	GCNNGC	2 cut(s)	1539, 2667
CaiI	CAGNNNCTG	2 cut(s)	911, 2591
CciI	TCATGA	1 cut(s)	2634
CfoI	GCGC	2 cut(s)	387, 428
Cfr13I	GGNCC	4 cut(s)	238, 368, 1719, 2749
ClaI	ATCGAT	2 cut(s)	897, 1947
CpoI	CGGWCCG	1 cut(s)	238
CseI	GACGC	2 cut(s)	427, 2684
CsiI	ACCWGGT	1 cut(s)	1498
Csp6I	GTAC	8 cut(s)	175, 181, 447, 713, 1620, 1713, 2415, 2896
CspI	CGGWCCG	1 cut(s)	238
CviQI	GTAC	8 cut(s)	175, 181, 447, 713, 1620, 1713, 2415, 2896
DpnI	GATC	8 cut(s)	19, 504, 900, 958, 1941, 1946, 1950, 2282
DpnII	GATC	8 cut(s)	17, 502, 898, 956, 1939, 1944, 1948, 2280
DraI	TTTAAA	2 cut(s)	1260, 1680
EaeI	YGGCCR	1 cut(s)	2667
Eam1104I	CTCTTC	6 cut(s)	750, 1131, 1260, 1277, 1524, 2771
EarI	CTCTTC	6 cut(s)	750, 1131, 1260, 1277, 1524, 2771
Ecl136II	GAGCTC	4 cut(s)	129, 918, 2562, 2602
Eco105I	TACGTA	1 cut(s)	1253
Eco130I	CCWWGG	1 cut(s)	274
Eco24I	GRGCYC	5 cut(s)	131, 920, 2366, 2564, 2604
Eco31I	GGTCTC	2 cut(s)	1462, 2984
Eco47I	GGWCC	3 cut(s)	238, 1719, 2749
Eco53kI	GAGCTC	4 cut(s)	129, 918, 2562, 2602
Eco57I	CTGAAG	6 cut(s)	129, 1664, 1871, 2030, 2211, 2507
Eco88I	CYCGRG	1 cut(s)	259
Eco91I	GGTNACC	1 cut(s)	466
EcoICRI	GAGCTC	4 cut(s)	129, 918, 2562, 2602
EcoO109I	RGGNCCY	1 cut(s)	1719
EcoO65I	GGTNACC	1 cut(s)	466
EcoRII	CCWGG	3 cut(s)	514, 1498, 1899
EcoT14I	CCWWGG	1 cut(s)	274
EcoT22I	ATGCAT	2 cut(s)	352, 498
EcoT38I	GRGCYC	5 cut(s)	131, 920, 2366, 2564, 2604
ErhI	CCWWGG	1 cut(s)	274
FalI	AAGNNNNNCTT	4 cut(s)	1521, 1553, 2445, 2477
FaqI	GGGAC	3 cut(s)	1577, 1732, 1917
FauI	CCCGC	3 cut(s)	470, 1554, 3007
FauNDI	CATATG	2 cut(s)	352, 460
FbaI	TGATCA	2 cut(s)	502, 1939
FokI	GGATG	8 cut(s)	542, 554, 1589, 2063, 2161, 2414, 2453, 2659
FriOI	GRGCYC	5 cut(s)	131, 920, 2366, 2564, 2604
FspBI	CTAG	7 cut(s)	986, 1761, 1866, 1923, 2270, 2345, 2579
GlaI	GCGC	2 cut(s)	386, 427
GsaI	CCCAGC	2 cut(s)	725, 1323
GsuI	CTGGAG	2 cut(s)	117, 771
HaeII	RGCGCY	1 cut(s)	429
HaeIII	GGCC	2 cut(s)	369, 2669
HgaI	GACGC	2 cut(s)	427, 2684
HhaI	GCGC	2 cut(s)	387, 428
Hin1I	GRCGYC	2 cut(s)	5, 438
Hin6I	GCGC	2 cut(s)	385, 426
HinP1I	GCGC	2 cut(s)	385, 426
HincII	GTYRAC	4 cut(s)	442, 1008, 1440, 2165
HindII	GTYRAC	4 cut(s)	442, 1008, 1440, 2165
HindIII	AAGCTT	1 cut(s)	1840
HphI	GGTGA	6 cut(s)	478, 512, 875, 1484, 1538, 2803
Hpy166II	GTNNAC	8 cut(s)	251, 442, 1008, 1082, 1440, 1766, 2165, 2415
Hpy188III	TCNNGA	8 cut(s)	575, 1383, 1610, 1812, 1889, 2034, 2379, 2635
Hpy8I	GTNNAC	8 cut(s)	251, 442, 1008, 1082, 1440, 1766, 2165, 2415
Hpy99I	CGWCG	1 cut(s)	2700
HpyCH4III	ACNGT	9 cut(s)	174, 232, 238, 446, 994, 1111, 1278, 2412, 2526
HpyCH4IV	ACGT	3 cut(s)	225, 1252, 1702
HpyF10VI	GCNNNNNNNGC	7 cut(s)	166, 296, 325, 1169, 1412, 2588, 2666
HpySE526I	ACGT	3 cut(s)	225, 1252, 1702
Hsp92I	GRCGYC	2 cut(s)	5, 438
HspAI	GCGC	2 cut(s)	385, 426
Ksp22I	TGATCA	2 cut(s)	502, 1939
Kzo9I	GATC	8 cut(s)	17, 502, 898, 956, 1939, 1944, 1948, 2280
LguI	GCTCTTC	1 cut(s)	750
LmnI	GCTCC	5 cut(s)	804, 1344, 1534, 1960, 2200
LweI	GCATC	8 cut(s)	80, 146, 532, 561, 661, 1306, 2183, 2681
MabI	ACCWGGT	1 cut(s)	1498
MaeI	CTAG	7 cut(s)	986, 1761, 1866, 1923, 2270, 2345, 2579
MaeII	ACGT	3 cut(s)	225, 1252, 1702
MaeIII	GTNAC	8 cut(s)	226, 232, 466, 1111, 1221, 1294, 2371, 2735
MalI	GATC	8 cut(s)	19, 504, 900, 958, 1941, 1946, 1950, 2282
MboI	GATC	8 cut(s)	17, 502, 898, 956, 1939, 1944, 1948, 2280
MfeI	CAATTG	3 cut(s)	884, 1935, 2204
MflI	RGATCY	1 cut(s)	956
MhlI	GDGCHC	9 cut(s)	131, 683, 762, 920, 1768, 2008, 2366, 2564, 2604
MlyI	GAGTC	8 cut(s)	478, 559, 1654, 1865, 2147, 2522, 2743, 2972
MmeI	TCCRAC	2 cut(s)	2418, 2705
Mph1103I	ATGCAT	2 cut(s)	352, 498
MroXI	GAANNNNTTC	6 cut(s)	1516, 1842, 1884, 2219, 2386, 2457
MslI	CAYNNNNRTG	5 cut(s)	314, 393, 765, 1089, 2001
MspA1I	CMGCKG	3 cut(s)	477, 749, 2588
MspR9I	CCNGG	3 cut(s)	516, 1500, 1901
MunI	CAATTG	3 cut(s)	884, 1935, 2204
Mva1269I	GAATGC	2 cut(s)	2294, 2809
MvaI	CCWGG	3 cut(s)	516, 1500, 1901
MvnI	CGCG	1 cut(s)	385
MwoI	GCNNNNNNNGC	7 cut(s)	166, 296, 325, 1169, 1412, 2588, 2666
NdeI	CATATG	2 cut(s)	352, 460
NdeII	GATC	8 cut(s)	17, 502, 898, 956, 1939, 1944, 1948, 2280
NlaIV	GGNNCC	4 cut(s)	806, 958, 1720, 2540
NmuCI	GTSAC	7 cut(s)	226, 232, 466, 1221, 1294, 2371, 2735
NsiI	ATGCAT	2 cut(s)	352, 498
NspI	RCATGY	3 cut(s)	416, 770, 2923
NspV	TTCGAA	1 cut(s)	2223
OliI	CACNNNNGTG	1 cut(s)	314
PagI	TCATGA	1 cut(s)	2634
PciI	ACATGT	1 cut(s)	2919
PciSI	GCTCTTC	1 cut(s)	750
PcsI	WCGNNNNNNNCGW	1 cut(s)	2981
PctI	GAATGC	2 cut(s)	2294, 2809
PdmI	GAANNNNTTC	6 cut(s)	1516, 1842, 1884, 2219, 2386, 2457
PfeI	GAWTC	6 cut(s)	637, 1043, 1428, 1808, 2876, 2969
PflFI	GACNNNGTC	1 cut(s)	2512
PflMI	CCANNNNNTGG	1 cut(s)	2922
PleI	GAGTC	8 cut(s)	478, 559, 1654, 1864, 2146, 2521, 2742, 2972
PpsI	GAGTC	8 cut(s)	478, 559, 1654, 1864, 2146, 2521, 2742, 2972
Ppu21I	YACGTR	1 cut(s)	1253
PpuMI	RGGWCCY	1 cut(s)	1719
PscI	ACATGT	1 cut(s)	2919
PshBI	ATTAAT	1 cut(s)	966
Psp124BI	GAGCTC	4 cut(s)	131, 920, 2564, 2604
Psp5II	RGGWCCY	1 cut(s)	1719
Psp6I	CCWGG	3 cut(s)	514, 1498, 1899
PspEI	GGTNACC	1 cut(s)	466
PspFI	CCCAGC	2 cut(s)	721, 1319
PspGI	CCWGG	3 cut(s)	514, 1498, 1899
PspN4I	GGNNCC	4 cut(s)	806, 958, 1720, 2540
PspPI	GGNCC	4 cut(s)	238, 368, 1719, 2749
PspPPI	RGGWCCY	1 cut(s)	1719
PstNI	CAGNNNCTG	2 cut(s)	911, 2591
PsuI	RGATCY	1 cut(s)	956
PsyI	GACNNNGTC	1 cut(s)	2512
PvuII	CAGCTG	2 cut(s)	749, 2588
RsaI	GTAC	8 cut(s)	176, 182, 448, 714, 1621, 1714, 2416, 2897
RsaNI	GTAC	8 cut(s)	175, 181, 447, 713, 1620, 1713, 2415, 2896
RseI	CAYNNNNRTG	5 cut(s)	314, 393, 765, 1089, 2001
Rsr2I	CGGWCCG	1 cut(s)	238
RsrII	CGGWCCG	1 cut(s)	238
SacI	GAGCTC	4 cut(s)	131, 920, 2564, 2604
SapI	GCTCTTC	1 cut(s)	750
Sau3AI	GATC	8 cut(s)	17, 502, 898, 956, 1939, 1944, 1948, 2280
Sau96I	GGNCC	4 cut(s)	238, 368, 1719, 2749
ScaI	AGTACT	2 cut(s)	176, 1621
SchI	GAGTC	8 cut(s)	478, 559, 1654, 1865, 2147, 2522, 2743, 2972
ScrFI	CCNGG	3 cut(s)	516, 1500, 1901
SduI	GDGCHC	9 cut(s)	131, 683, 762, 920, 1768, 2008, 2366, 2564, 2604
SexAI	ACCWGGT	1 cut(s)	1498
SfaNI	GCATC	8 cut(s)	80, 146, 532, 561, 661, 1306, 2183, 2681
SfuI	TTCGAA	1 cut(s)	2223
SinI	GGWCC	3 cut(s)	238, 1719, 2749
SmiMI	CAYNNNNRTG	5 cut(s)	314, 393, 765, 1089, 2001
SmlI	CTYRAG	4 cut(s)	1610, 1779, 2228, 2603
SmoI	CTYRAG	4 cut(s)	1610, 1779, 2228, 2603
SnaBI	TACGTA	1 cut(s)	1253
SpeI	ACTAGT	1 cut(s)	1760
SsiI	CCGC	9 cut(s)	197, 241, 477, 1561, 1784, 2014, 2670, 2789, 3014
SspI	AATATT	1 cut(s)	101
SspMI	CTAG	7 cut(s)	986, 1761, 1866, 1923, 2270, 2345, 2579
SstI	GAGCTC	4 cut(s)	131, 920, 2564, 2604
StyD4I	CCNGG	3 cut(s)	514, 1498, 1899
StyI	CCWWGG	1 cut(s)	274
TaaI	ACNGT	9 cut(s)	174, 232, 238, 446, 994, 1111, 1278, 2412, 2526
TaiI	ACGT	3 cut(s)	228, 1255, 1705
TatI	WGTACW	5 cut(s)	174, 180, 712, 1619, 2414
TauI	GCSGC	2 cut(s)	199, 2672
TfiI	GAWTC	6 cut(s)	637, 1043, 1428, 1808, 2876, 2969
TscAI	CASTG	6 cut(s)	235, 534, 1114, 1753, 1833, 2662
TseFI	GTSAC	7 cut(s)	226, 232, 466, 1221, 1294, 2371, 2735
Tsp45I	GTSAC	7 cut(s)	226, 232, 466, 1221, 1294, 2371, 2735
TspGWI	ACGGA	7 cut(s)	30, 961, 1711, 2482, 2525, 2846, 2970
TspRI	CASTG	6 cut(s)	235, 534, 1114, 1753, 1833, 2662
Tth111I	GACNNNGTC	1 cut(s)	2512
Van91I	CCANNNNNTGG	1 cut(s)	2922
VneI	GTGCAC	1 cut(s)	1764
VpaK11BI	GGWCC	3 cut(s)	238, 1719, 2749
VspI	ATTAAT	1 cut(s)	966
XapI	RAATTY	7 cut(s)	267, 652, 781, 1157, 1185, 1894, 2356
XceI	RCATGY	3 cut(s)	416, 770, 2923
XmnI	GAANNNNTTC	6 cut(s)	1516, 1842, 1884, 2219, 2386, 2457
XspI	CTAG	7 cut(s)	986, 1761, 1866, 1923, 2270, 2345, 2579
ZrmI	AGTACT	2 cut(s)	176, 1621
Zsp2I	ATGCAT	2 cut(s)	352, 498

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.