Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0002243

Physical Location & Seq

Genomic Coordinates Forward (+)

5238456 .. 5249018

Gene Structure 10563 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1053100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3024 bp

ATGGCGTCAAAATATGGATCAAAATCAAAAAAATTAGGTTCGAGCAGTTCAAAGGCTGCTAATTCCCCTGCATCTTCGACTACAACGTCTTCAAAACAATATTTGGAAACATCTATTGAATGCCAGAGTTCTCCTGCGTCTTCTTCGGCTCGAAGTAAGCCACAGTACTTGTACTTGGAAAGTTTACCGCAAGATGTGGAAAAATCCAAAGAAAACGTCACTGTGACGGTCCGCTTTCGTCCACTCAGCCCGAGGGAAATTCGCCAAGAAGAAGAAATTGCATGGTATGCAGATGGCGACACCATTGTGCGAAATGAGCATAATCCATCAATAGCTTATGCATATGATCGGGTATTCGGCCCAACCACCACAACGCGCCATGTCTATGATGTTGCTGCTCAGCATGTTATTAGTGGTGCTATGGAAGGCGTCAATGGTACAATATTTGCGTATGGGGTGACCAGCAGCGGGAAGACTCACACTATGCATGGTGATCAAAGGTCGCCTGGAATTATACCACTGGCTGTGAAGGATGCTTTTAGCATCATCCAAGAGACTCCAAATCGAGAGTATCTTCTTCGTGTGTCATACTTGGAAATCTATAATGAGGTTGTAAATGACTTGTTGAATCCAGCAGGACAGAATTTACGAATTAGAGAAGATGCCCAGGGCACCTTTGTTGAAGGAATAAAGGAAGAAGTTGTACTGTCCCCAGCTCACGCCCTTTCACTTATAGCAGCTGGAGAAGAGCACAGGCATGTTGGCTCAACAAATTTCAATCTATTCAGTAGCAGGAGCCACACGATATTTACGCTGACAATAGAGAGTAGTCCATGTGGGGAAAATTGTGAAGGTGAAGCTGTAAGTCTGTCACAATTGAACCTCATCGATCTGGCAGGTTCTGAGAGCTCGAAAGCTGAAACTACTGGTGTCAGACGGAAAGAAGGATCCTATATTAATAGAAGTTTGCTTACTCTAGGAACTGTTATATCAAAGTTAACCGATATGAGGGCTACTCATATACCATACCGGGATTCTAAATTGACAAGACTTCTTCAATCTTCCCTAAGTGGACATGGGCGTGTATCTCTCATCTGCACTGTTACTCCTTCATCAAGCAGTTCCGAAGAGACGCATAATACATTGAAATTTGCACATCGGGCAAAACACATTGAAATTCAAGCAGCACAAAACAAGATTATTGATGAGAAGTCGCTCATTAAGAAATATCAAAATGAGATACGTAGTTTAAAGGAAGAGTTAGAAGAGTTGAAGAGGGGTATAGTCACAATTCCTCAACTAAAAGATGCTGGGGAAGATGATATTCTGCTCCTTAAACAAAAGTTAGAAGATGGCAAATACAAACTGCAATCAAGATTGGAACAAGAAGAAGAAGCCAAAGCTGCTTTGTTGGGAAGAATCCAAAGGCTGACAAAATTGATTTTGGTCTCAACAAAAGGAACACAATTTTCAAGGTTTTCCCACCGACCTGGTGTTAGAAGAAGACATTCCTTTGGTGAAGAGGAGCTTGCATACCTACCATACAAAAGGCGGGACTTGATTTTGGATGATGAAAGTGTTGAATTGTTTGTTCCTCCTCTTGAGGGGAGTACTGAAATGACTGATGACACACTGAAGGGGGAGAAGAAGACTCGGAAGCATGGACTTCTAAACTGGTTAAAGATACGGAAACGTGATAGTGGTACAGGGACGTTGACAAGCACAAGTGATAGATCCAGTGGAATAAAATCTACTATTACACCGTCAACACCTCAAGCAGAAAGCGGTAATTTTCATGTAGAATCCAGACTTTCACATTCAGCTCTTACAGAAAACTCTCCATTGGCTGATCTTTTGTTTGAGGCTAGAGAGGACAGAGAAGTTCGTCAGGAAAATTTCCTGGGACGAGAGACACCTCTGACTAGCATGAAGTCAATTGATCGGATTGATCTTTTAAGGGAGCAGCGAAAAATCTTGTCTGATGAGGTAGCACTCCGTTCAAGTGCTCTGAAGCGGTTGTCTGAGGAGGCTTCAAGACACTCCAAGAAGGATGAAATTAAGATGGAGATGCGAAAGTTCAAAGATGAAATCAAGGCGAAGAATGAGCAAATAGCTTTGGTGGAAAAGAAAATTGCAGAGTCCTTTTCACACAACAAAATGAGTCAACTGGAAGCATCCCAATCTATTGCTGAAGTGATGGAGCAATTGAATGAGAAGTCCTTTGAACTTGAGGTTAAAGCTGCTGATAATCGTATCATCCAAGAACAGCTGGAACAAAAGATTGGTGAATGCAAAGGATTGCAGGAAACAGTCGCCTCCTTGAAGCAGCAGCTTTCTGAGGCACTAGAGTTTAGAAATTTGAGCCCAATAGTCACTTCTCGAATTGGTTCAAAAAATTTGCATGAGGAACCAGGCACAGAGAAAGATAATGCGGTGTTGAATGACAAAAATGAAGTTTTCCTTCTACGGAAGCAGGTTGAAGAACTGAAGCAGAAGGTGGCTGAACTGACGGAGTCAAAGGAACAGTTAGAAGTTCGGAACAAGAAATTGGTGGAGGAGAGCTTGTACGCCAAAGGTCTAGCCTCAGCTGCTGCTGTTGAGCTCAAGGCATTATCAGAAGAAGTTTCCAAACTCATGAACCAAAATGAAAAACAAGCTGCTGAGCTAGCCGCATCTAAGAGCTCTCCAACTCTGCGTCGAAATAGTAGCACAATCCGAAATGGTCGGAGAGATAGTCACATCAAACAGGACCAAAGCGGGCTTATCTCAGAAATGAAGAGTGAACTGGCAGTGAGCAAGGAGAGAGAGCATTCATACGAGGCTGCTCTTATGGAGAAGGATAAAAGAGAGGCTGATCTTCAAAGAAGGGTTGAGGAATCCAAGCAAAGAGAAGCATACCTGGAAAATGAACTCGCCAACATGTGGGTTCTCGTGGCGAAATTAAAAAAATCCCACGGAACTGAAACTGATTCTTCGGACTCAACAAAAGAGACCCGACAAAATGATGGTTATGGGGGATGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005874 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032991 GO:0035371 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:1990752

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin

Protein Analysis

1008

Amino Acids

112.92

Weight (kDa)

6.73

Isoelectric Point (pI)

54.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	66 - 209	8.9e-20	Microtubule binding
Kinesin	PF00225	78 - 391	4.2e-105	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002243)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12020 AT3G12020 AT3G12020 AT3G12020 AT5G06670 AT5G06670 AT5G06670 AT5G06670
fragaria_vesca	FvH4_6g21780 FvH4_6g21780 FvH4_6g21780 FvH4_6g21780 FvH4_6g21780 FvH4_6g21780 FvH4_6g21780 FvH4_6g21780 FvH4_6g21780
malus_domestica	MD12G1054300.v1.1 MD14G1053100.v1.1
prunus_persica	Prupe.7G082400_v2.0.a1
pyrus_communis	pycom12g04730 pycom14g04410
rosa_chinensis	RchiOBHm_Chr3g0479141
rosa_laevigata	RLG00000023604
rosa_multiflora	Rmu_co8049936.1_g000001 Rmu_co8383491.1_g000001 Rmu_sc0002555.1_g000005
rosa_roxburghii	Rroxscaffold_6G00402700
rosa_rugosa	Rorug03G0171300 Rorug03G0171400
rosa_samantha	Rh3AG222600 Rh3BG255100 Rh3CG250700 Rh3DG247100 Rh6AG090700 Rh6BG153100 Rh6CG078500 Rh6DG074300 Rh6DG137500
rosa_wichuraiana	Rw0G001170 Rw1G014040 Rw3G019950 Rw6G024570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	85
Acc36I	ACCTGC	2 cut(s)	887, 2464
AccB1I	GGYRCC	1 cut(s)	671
AccB7I	CCANNNNNTGG	1 cut(s)	2922
AccII	CGCG	1 cut(s)	376
AciI	CCGC	9 cut(s)	188, 232, 468, 1552, 1785, 2014, 2432, 2670, 2757
AclWI	GGATC	4 cut(s)	25, 942, 955, 1728
AcsI	RAATTY	8 cut(s)	258, 643, 772, 1148, 1176, 1894, 2356, 2395
AcuI	CTGAAG	4 cut(s)	1655, 2030, 2211, 2507
AcyI	GRCGYC	2 cut(s)	5, 429
AfaI	GTAC	7 cut(s)	167, 173, 439, 705, 1612, 1705, 2567
AfiI	CCNNNNNNNGG	4 cut(s)	468, 1008, 1604, 2922
AflIII	ACRYGT	1 cut(s)	2919
AjnI	CCWGG	6 cut(s)	505, 666, 1489, 1899, 2410, 2898
AjuI	GAANNNNNNNTTGG	6 cut(s)	2265, 2297, 2359, 2391, 2531, 2563
AleI	CACNNNNGTG	1 cut(s)	305
Alw21I	GWGCWC	5 cut(s)	753, 911, 2008, 2604, 2684
Alw26I	GTCTC	5 cut(s)	548, 1124, 1453, 1904, 2984
AlwI	GGATC	4 cut(s)	25, 942, 955, 1728
AlwNI	CAGNNNCTG	2 cut(s)	902, 2591
Ama87I	CYCGRG	1 cut(s)	250
AoxI	GGCC	1 cut(s)	358
ApoI	RAATTY	8 cut(s)	258, 643, 772, 1148, 1176, 1894, 2356, 2395
ArsI	GACNNNNNNTTYG	2 cut(s)	201, 233
AseI	ATTAAT	1 cut(s)	957
Asp700I	GAANNNNTTC	2 cut(s)	1507, 2386
AspLEI	GCGC	1 cut(s)	378
AspS9I	GGNCC	3 cut(s)	229, 359, 2749
AsuC2I	CCSGG	1 cut(s)	1031
AsuHPI	GGTGA	5 cut(s)	469, 503, 866, 1529, 2297
AsuNHI	GCTAGC	1 cut(s)	2665
AvaI	CYCGRG	1 cut(s)	250
AvaII	GGWCC	2 cut(s)	229, 2749
BaeGI	GKGCMC	1 cut(s)	674
BamHI	GGATCC	1 cut(s)	947
BanI	GGYRCC	1 cut(s)	671
BanII	GRGCYC	4 cut(s)	911, 2366, 2604, 2684
BarI	GAAGNNNNNNTAC	4 cut(s)	687, 719, 955, 987
BauI	CACGAG	1 cut(s)	2930
BbsI	GAAGAC	5 cut(s)	81, 132, 479, 1510, 1655
Bbv12I	GWGCWC	5 cut(s)	753, 911, 2008, 2604, 2684
BbvCI	CCTCAGC	1 cut(s)	2584
BccI	CCATC	7 cut(s)	287, 334, 1346, 2056, 2191, 2999, 3012
BciT130I	CCWGG	6 cut(s)	507, 668, 1491, 1901, 2412, 2900
BclI	TGATCA	1 cut(s)	493
BcnI	CCSGG	1 cut(s)	1031
BcoDI	GTCTC	5 cut(s)	548, 1124, 1453, 1904, 2984
BfaI	CTAG	6 cut(s)	977, 1866, 1923, 2345, 2579, 2666
BfuAI	ACCTGC	2 cut(s)	887, 2464
BlpI	GCTNAGC	2 cut(s)	399, 2661
BmcAI	AGTACT	2 cut(s)	167, 1612
Bme1390I	CCNGG	7 cut(s)	507, 668, 1031, 1491, 1901, 2412, 2900
Bme18I	GGWCC	2 cut(s)	229, 2749
BmeT110I	CYCGRG	1 cut(s)	250
BmgT120I	GGNCC	3 cut(s)	229, 359, 2749
BmiI	GGNNCC	4 cut(s)	673, 797, 949, 2409
BmrFI	CCNGG	7 cut(s)	507, 668, 1031, 1491, 1901, 2412, 2900
BmsI	GCATC	8 cut(s)	80, 523, 552, 652, 1297, 2058, 2183, 2681
BmtI	GCTAGC	1 cut(s)	2669
BpiI	GAAGAC	5 cut(s)	81, 132, 479, 1510, 1655
BplI	GAGNNNNNCTC	2 cut(s)	1000, 1032
BpmI	CTGGAG	1 cut(s)	762
Bpu10I	CCTNAGC	1 cut(s)	2584
Bpu1102I	GCTNAGC	2 cut(s)	399, 2661
BpuEI	CTTGAG	4 cut(s)	1622, 1758, 2249, 2588
BpuMI	CCSGG	1 cut(s)	1031
Bsa29I	ATCGAT	1 cut(s)	888
BsaAI	YACGTR	1 cut(s)	1244
BsaBI	GATNNNNATC	1 cut(s)	22
BsaHI	GRCGYC	2 cut(s)	5, 429
BsaI	GGTCTC	2 cut(s)	1453, 2984
BsaJI	CCNNGG	5 cut(s)	251, 666, 667, 1900, 2953
BsaXI	ACNNNNNCTCC	6 cut(s)	1090, 1120, 2549, 2579, 2719, 2749
Bsc4I	CCNNNNNNNGG	4 cut(s)	468, 1008, 1604, 2922
Bse1I	ACTGG	6 cut(s)	525, 931, 1679, 1737, 2172, 2790
Bse8I	GATNNNNATC	1 cut(s)	22
BseBI	CCWGG	6 cut(s)	507, 668, 1491, 1901, 2412, 2900
BseCI	ATCGAT	1 cut(s)	888
BseDI	CCNNGG	5 cut(s)	251, 666, 667, 1900, 2953
BseGI	GGATG	7 cut(s)	538, 546, 1573, 2056, 2174, 2256, 3023
BseJI	GATNNNNATC	1 cut(s)	22
BseLI	CCNNNNNNNGG	4 cut(s)	468, 1008, 1604, 2922
BseMII	CTCAG	8 cut(s)	259, 413, 894, 2013, 2328, 2598, 2652, 2781
BseNI	ACTGG	6 cut(s)	525, 931, 1679, 1737, 2172, 2790
BseRI	GAGGAG	4 cut(s)	1538, 1587, 2039, 2570
BseSI	GKGCMC	1 cut(s)	674
BseYI	CCCAGC	2 cut(s)	712, 1310
BsgI	GTGCAG	1 cut(s)	1081
Bsh1236I	CGCG	1 cut(s)	376
BshFI	GGCC	1 cut(s)	360
BshNI	GGYRCC	1 cut(s)	671
BshVI	ATCGAT	1 cut(s)	888
BsiHKAI	GWGCWC	5 cut(s)	753, 911, 2008, 2604, 2684
BsiHKCI	CYCGRG	1 cut(s)	250
BsiSI	CCGG	1 cut(s)	1030
BslFI	GGGAC	4 cut(s)	694, 1568, 1723, 1917
BslI	CCNNNNNNNGG	4 cut(s)	468, 1008, 1604, 2922
BsmAI	GTCTC	5 cut(s)	548, 1124, 1453, 1904, 2984
BsmBI	CGTCTC	1 cut(s)	1124
BsmFI	GGGAC	4 cut(s)	694, 1568, 1723, 1917
BsmI	GAATGC	3 cut(s)	125, 2294, 2809
BsnI	GGCC	1 cut(s)	360
Bso31I	GGTCTC	2 cut(s)	1453, 2984
BsoBI	CYCGRG	1 cut(s)	250
Bsp1286I	GDGCHC	7 cut(s)	674, 753, 911, 2008, 2366, 2604, 2684
Bsp1720I	GCTNAGC	2 cut(s)	399, 2661
BspACI	CCGC	9 cut(s)	188, 232, 468, 1552, 1785, 2014, 2432, 2670, 2757
BspANI	GGCC	1 cut(s)	360
BspCNI	CTCAG	8 cut(s)	258, 412, 895, 2014, 2329, 2597, 2653, 2780
BspDI	ATCGAT	1 cut(s)	888
BspFNI	CGCG	1 cut(s)	376
BspHI	TCATGA	1 cut(s)	2634
BspLI	GGNNCC	4 cut(s)	673, 797, 949, 2409
BspMI	ACCTGC	2 cut(s)	887, 2464
BspOI	GCTAGC	1 cut(s)	2669
BspPI	GGATC	4 cut(s)	25, 942, 955, 1728
BspQI	GCTCTTC	1 cut(s)	741
BspT107I	GGYRCC	1 cut(s)	671
BspTNI	GGTCTC	2 cut(s)	1453, 2984
BsrI	ACTGG	6 cut(s)	525, 931, 1679, 1737, 2172, 2790
BssECI	CCNNGG	5 cut(s)	251, 666, 667, 1900, 2953
BssNI	GRCGYC	2 cut(s)	5, 429
BssSI	CACGAG	1 cut(s)	2930
Bst2BI	CACGAG	1 cut(s)	2930
Bst2UI	CCWGG	6 cut(s)	507, 668, 1491, 1901, 2412, 2900
Bst4CI	ACNGT	9 cut(s)	165, 223, 229, 708, 985, 1102, 1764, 2311, 2526
Bst6I	CTCTTC	7 cut(s)	741, 1122, 1251, 1260, 1268, 1515, 2771
BstACI	GRCGYC	2 cut(s)	5, 429
BstAPI	GCANNNNNTGC	1 cut(s)	287
BstBAI	YACGTR	1 cut(s)	1244
BstC8I	GCNNGC	3 cut(s)	1530, 2667, 2759
BstDSI	CCRYGG	1 cut(s)	2953
BstEII	GGTNACC	1 cut(s)	457
BstF5I	GGATG	7 cut(s)	538, 546, 1573, 2056, 2174, 2256, 3023
BstFNI	CGCG	1 cut(s)	376
BstHHI	GCGC	1 cut(s)	378
BstMAI	GTCTC	5 cut(s)	548, 1124, 1453, 1904, 2984
BstMWI	GCNNNNNNNGC	6 cut(s)	287, 316, 1160, 1403, 2588, 2666
BstNI	CCWGG	6 cut(s)	507, 668, 1491, 1901, 2412, 2900
BstNSI	RCATGY	3 cut(s)	407, 761, 2923
BstPI	GGTNACC	1 cut(s)	457
BstSCI	CCNGG	7 cut(s)	505, 666, 1029, 1489, 1899, 2410, 2898
BstSLI	GKGCMC	1 cut(s)	674
BstSNI	TACGTA	1 cut(s)	1244
BstUI	CGCG	1 cut(s)	376
BstV2I	GAAGAC	5 cut(s)	81, 132, 479, 1510, 1655
BstX2I	RGATCY	2 cut(s)	947, 1733
BstXI	CCANNNNNNTGG	1 cut(s)	1490
BstYI	RGATCY	2 cut(s)	947, 1733
Bsu15I	ATCGAT	1 cut(s)	888
BsuRI	GGCC	1 cut(s)	360
BsuTUI	ATCGAT	1 cut(s)	888
BtgI	CCRYGG	1 cut(s)	2953
BtsCI	GGATG	7 cut(s)	538, 546, 1573, 2056, 2174, 2256, 3023
BtsI	GCAGTG	1 cut(s)	2796
BtsIMutI	CAGTG	6 cut(s)	219, 518, 1098, 1631, 1744, 2796
BveI	ACCTGC	2 cut(s)	887, 2464
Cac8I	GCNNGC	3 cut(s)	1530, 2667, 2759
CaiI	CAGNNNCTG	2 cut(s)	902, 2591
CciI	TCATGA	1 cut(s)	2634
CfoI	GCGC	1 cut(s)	378
Cfr13I	GGNCC	3 cut(s)	229, 359, 2749
ClaI	ATCGAT	1 cut(s)	888
CpoI	CGGWCCG	1 cut(s)	229
CseI	GACGC	4 cut(s)	126, 418, 1141, 2684
CsiI	ACCWGGT	1 cut(s)	1489
Csp6I	GTAC	7 cut(s)	166, 172, 438, 704, 1611, 1704, 2566
CspCI	CAANNNNNGTGG	2 cut(s)	150, 185
CspI	CGGWCCG	1 cut(s)	229
CviQI	GTAC	7 cut(s)	166, 172, 438, 704, 1611, 1704, 2566
DraI	TTTAAA	1 cut(s)	1251
DrdI	GACNNNNNNGTC	1 cut(s)	85
DseDI	GACNNNNNNGTC	1 cut(s)	85
Eam1104I	CTCTTC	7 cut(s)	741, 1122, 1251, 1260, 1268, 1515, 2771
EarI	CTCTTC	7 cut(s)	741, 1122, 1251, 1260, 1268, 1515, 2771
Ecl136II	GAGCTC	3 cut(s)	909, 2602, 2682
Eco105I	TACGTA	1 cut(s)	1244
Eco24I	GRGCYC	4 cut(s)	911, 2366, 2604, 2684
Eco31I	GGTCTC	2 cut(s)	1453, 2984
Eco47I	GGWCC	2 cut(s)	229, 2749
Eco53kI	GAGCTC	3 cut(s)	909, 2602, 2682
Eco57I	CTGAAG	4 cut(s)	1655, 2030, 2211, 2507
Eco88I	CYCGRG	1 cut(s)	250
Eco91I	GGTNACC	1 cut(s)	457
EcoICRI	GAGCTC	3 cut(s)	909, 2602, 2682
EcoO65I	GGTNACC	1 cut(s)	457
EcoRII	CCWGG	6 cut(s)	505, 666, 1489, 1899, 2410, 2898
EcoT22I	ATGCAT	2 cut(s)	343, 489
EcoT38I	GRGCYC	4 cut(s)	911, 2366, 2604, 2684
Esp3I	CGTCTC	1 cut(s)	1124
FalI	AAGNNNNNCTT	4 cut(s)	1512, 1544, 2445, 2477
FaqI	GGGAC	4 cut(s)	694, 1568, 1723, 1917
FauI	CCCGC	3 cut(s)	461, 1545, 2750
FauNDI	CATATG	1 cut(s)	343
FbaI	TGATCA	1 cut(s)	493
FokI	GGATG	6 cut(s)	533, 545, 1580, 2063, 2161, 2243
FriOI	GRGCYC	4 cut(s)	911, 2366, 2604, 2684
FspBI	CTAG	6 cut(s)	977, 1866, 1923, 2345, 2579, 2666
GlaI	GCGC	1 cut(s)	377
GsaI	CCCAGC	2 cut(s)	716, 1314
GsuI	CTGGAG	1 cut(s)	762
HaeIII	GGCC	1 cut(s)	360
HapII	CCGG	1 cut(s)	1030
HgaI	GACGC	4 cut(s)	126, 418, 1141, 2684
HhaI	GCGC	1 cut(s)	378
Hin1I	GRCGYC	2 cut(s)	5, 429
Hin6I	GCGC	1 cut(s)	376
HinP1I	GCGC	1 cut(s)	376
HincII	GTYRAC	4 cut(s)	999, 1716, 1767, 2165
HindII	GTYRAC	4 cut(s)	999, 1716, 1767, 2165
HpaI	GTTAAC	1 cut(s)	999
HpaII	CCGG	1 cut(s)	1030
HphI	GGTGA	5 cut(s)	469, 503, 866, 1529, 2297
Hpy166II	GTNNAC	8 cut(s)	185, 242, 999, 1073, 1716, 1767, 2165, 2783
Hpy188III	TCNNGA	8 cut(s)	566, 1374, 1601, 1806, 1889, 2034, 2379, 2635
Hpy8I	GTNNAC	8 cut(s)	185, 242, 999, 1073, 1716, 1767, 2165, 2783
Hpy99I	CGWCG	1 cut(s)	2700
HpyCH4III	ACNGT	9 cut(s)	165, 223, 229, 708, 985, 1102, 1764, 2311, 2526
HpyCH4IV	ACGT	5 cut(s)	86, 216, 1243, 1693, 1712
HpyF10VI	GCNNNNNNNGC	6 cut(s)	287, 316, 1160, 1403, 2588, 2666
HpySE526I	ACGT	5 cut(s)	86, 216, 1243, 1693, 1712
Hsp92I	GRCGYC	2 cut(s)	5, 429
HspAI	GCGC	1 cut(s)	376
Ksp22I	TGATCA	1 cut(s)	493
KspAI	GTTAAC	1 cut(s)	999
LguI	GCTCTTC	1 cut(s)	741
LmnI	GCTCC	5 cut(s)	795, 1335, 1525, 1960, 2200
LweI	GCATC	8 cut(s)	80, 523, 552, 652, 1297, 2058, 2183, 2681
MabI	ACCWGGT	1 cut(s)	1489
MaeI	CTAG	6 cut(s)	977, 1866, 1923, 2345, 2579, 2666
MaeII	ACGT	5 cut(s)	86, 216, 1243, 1693, 1712
MaeIII	GTNAC	8 cut(s)	217, 223, 457, 870, 1102, 1285, 2371, 2735
MfeI	CAATTG	3 cut(s)	875, 1935, 2204
MflI	RGATCY	2 cut(s)	947, 1733
MhlI	GDGCHC	7 cut(s)	674, 753, 911, 2008, 2366, 2604, 2684
MlyI	GAGTC	7 cut(s)	469, 550, 1645, 2147, 2170, 2522, 2972
MmeI	TCCRAC	2 cut(s)	2705, 2711
Mph1103I	ATGCAT	2 cut(s)	343, 489
MroXI	GAANNNNTTC	2 cut(s)	1507, 2386
MslI	CAYNNNNRTG	4 cut(s)	305, 384, 756, 1080
MspA1I	CMGCKG	4 cut(s)	468, 740, 2269, 2588
MspI	CCGG	1 cut(s)	1030
MspR9I	CCNGG	7 cut(s)	507, 668, 1031, 1491, 1901, 2412, 2900
MunI	CAATTG	3 cut(s)	875, 1935, 2204
Mva1269I	GAATGC	3 cut(s)	125, 2294, 2809
MvaI	CCWGG	6 cut(s)	507, 668, 1491, 1901, 2412, 2900
MvnI	CGCG	1 cut(s)	376
MwoI	GCNNNNNNNGC	6 cut(s)	287, 316, 1160, 1403, 2588, 2666
NciI	CCSGG	1 cut(s)	1031
NdeI	CATATG	1 cut(s)	343
NheI	GCTAGC	1 cut(s)	2665
NlaIV	GGNNCC	4 cut(s)	673, 797, 949, 2409
NmuCI	GTSAC	7 cut(s)	217, 223, 457, 870, 1285, 2371, 2735
NsiI	ATGCAT	2 cut(s)	343, 489
NspI	RCATGY	3 cut(s)	407, 761, 2923
OliI	CACNNNNGTG	1 cut(s)	305
PagI	TCATGA	1 cut(s)	2634
PasI	CCCWGGG	1 cut(s)	667
PciI	ACATGT	1 cut(s)	2919
PciSI	GCTCTTC	1 cut(s)	741
PcsI	WCGNNNNNNNCGW	1 cut(s)	83
PctI	GAATGC	3 cut(s)	125, 2294, 2809
PdmI	GAANNNNTTC	2 cut(s)	1507, 2386
PfeI	GAWTC	6 cut(s)	628, 1034, 1419, 1802, 2876, 2969
PflFI	GACNNNGTC	1 cut(s)	2512
PflMI	CCANNNNNTGG	1 cut(s)	2922
PleI	GAGTC	7 cut(s)	469, 550, 1645, 2146, 2169, 2521, 2972
PpsI	GAGTC	7 cut(s)	469, 550, 1645, 2146, 2169, 2521, 2972
Ppu21I	YACGTR	1 cut(s)	1244
PscI	ACATGT	1 cut(s)	2919
PshBI	ATTAAT	1 cut(s)	957
Psp124BI	GAGCTC	3 cut(s)	911, 2604, 2684
Psp6I	CCWGG	6 cut(s)	505, 666, 1489, 1899, 2410, 2898
PspEI	GGTNACC	1 cut(s)	457
PspFI	CCCAGC	2 cut(s)	712, 1310
PspGI	CCWGG	6 cut(s)	505, 666, 1489, 1899, 2410, 2898
PspN4I	GGNNCC	4 cut(s)	673, 797, 949, 2409
PspPI	GGNCC	3 cut(s)	229, 359, 2749
PstNI	CAGNNNCTG	2 cut(s)	902, 2591
PsuI	RGATCY	2 cut(s)	947, 1733
PsyI	GACNNNGTC	1 cut(s)	2512
PvuII	CAGCTG	3 cut(s)	740, 2269, 2588
RsaI	GTAC	7 cut(s)	167, 173, 439, 705, 1612, 1705, 2567
RsaNI	GTAC	7 cut(s)	166, 172, 438, 704, 1611, 1704, 2566
RseI	CAYNNNNRTG	4 cut(s)	305, 384, 756, 1080
Rsr2I	CGGWCCG	1 cut(s)	229
RsrII	CGGWCCG	1 cut(s)	229
SacI	GAGCTC	3 cut(s)	911, 2604, 2684
SapI	GCTCTTC	1 cut(s)	741
Sau96I	GGNCC	3 cut(s)	229, 359, 2749
ScaI	AGTACT	2 cut(s)	167, 1612
SchI	GAGTC	7 cut(s)	469, 550, 1645, 2147, 2170, 2522, 2972
ScrFI	CCNGG	7 cut(s)	507, 668, 1031, 1491, 1901, 2412, 2900
SduI	GDGCHC	7 cut(s)	674, 753, 911, 2008, 2366, 2604, 2684
SexAI	ACCWGGT	1 cut(s)	1489
SfaNI	GCATC	8 cut(s)	80, 523, 552, 652, 1297, 2058, 2183, 2681
SinI	GGWCC	2 cut(s)	229, 2749
SmiMI	CAYNNNNRTG	4 cut(s)	305, 384, 756, 1080
SmlI	CTYRAG	4 cut(s)	1601, 1773, 2228, 2603
SmoI	CTYRAG	4 cut(s)	1601, 1773, 2228, 2603
SnaBI	TACGTA	1 cut(s)	1244
SsiI	CCGC	9 cut(s)	188, 232, 468, 1552, 1785, 2014, 2432, 2670, 2757
SspI	AATATT	2 cut(s)	101, 444
SspMI	CTAG	6 cut(s)	977, 1866, 1923, 2345, 2579, 2666
SstI	GAGCTC	3 cut(s)	911, 2604, 2684
StyD4I	CCNGG	7 cut(s)	505, 666, 1029, 1489, 1899, 2410, 2898
TaaI	ACNGT	9 cut(s)	165, 223, 229, 708, 985, 1102, 1764, 2311, 2526
TaiI	ACGT	5 cut(s)	89, 219, 1246, 1696, 1715
TaqI	TCGA	8 cut(s)	41, 77, 151, 565, 888, 911, 2380, 2698
TatI	WGTACW	4 cut(s)	165, 171, 703, 1610
TauI	GCSGC	1 cut(s)	2672
TfiI	GAWTC	6 cut(s)	628, 1034, 1419, 1802, 2876, 2969
TscAI	CASTG	6 cut(s)	226, 525, 1105, 1638, 1744, 2796
TseFI	GTSAC	7 cut(s)	217, 223, 457, 870, 1285, 2371, 2735
Tsp45I	GTSAC	7 cut(s)	217, 223, 457, 870, 1285, 2371, 2735
TspGWI	ACGGA	6 cut(s)	952, 1702, 1985, 2482, 2525, 2970
TspRI	CASTG	6 cut(s)	226, 525, 1105, 1638, 1744, 2796
Tth111I	GACNNNGTC	1 cut(s)	2512
Van91I	CCANNNNNTGG	1 cut(s)	2922
VpaK11BI	GGWCC	2 cut(s)	229, 2749
VspI	ATTAAT	1 cut(s)	957
XapI	RAATTY	8 cut(s)	258, 643, 772, 1148, 1176, 1894, 2356, 2395
XceI	RCATGY	3 cut(s)	407, 761, 2923
XmnI	GAANNNNTTC	2 cut(s)	1507, 2386
XspI	CTAG	6 cut(s)	977, 1866, 1923, 2345, 2579, 2666
ZrmI	AGTACT	2 cut(s)	167, 1612
Zsp2I	ATGCAT	2 cut(s)	343, 489

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.