Rh6BG153100
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0002243
Physical Location & Seq
Forward (+)
24666914 .. 24670895
3982 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG153100.1

Sequence Viewer

Length: 1083 bp
ATGGGTGTGGGCACATTTGTTGAAGGAATAAAGGAAGAAGTAGTGCTATCCCCAGCTCATGCCCTGTCACTTATAGCAGCTGGAGAAGAGCACAGGCATGTTGGATCAACAAATTTCAATTTACTCAGTAGCAGGAGCCATACTATATTTACACTGACAATCGAGAGTAGCCCGTGTGGGGAAAATAGTGAAGGTGAAGCTGTAAATCTGTCGCAATTGAACCTCATTGATCTGGCTGGTTCTGAGAGCTCAAAAGCTGAAACTACTGGTGTCAGACGAAAAGAAGGATCCTATATTAATAAGAGTCTGCTAACTCTAGGAACTGTTATATCAAAGTTGACGGATGGGAGGGCTACTCATATACCCTACAGGGATTCTAAACTGACCCGACTTCTCCAATCTTCACTAAGTGGTCATGGGCGTGTCTCTCTCATCTGCACTGTTACTCCTTCATCAAGCAGTTCAGAAGAGACGCATAATACATTAAAGTTTGCGCACCGGGCAAAGCACATTGAAATTCAAGCAGCACAAAATAAGATTATTGATGAGAAGTCACTTATCAAGAAATATCAAAATGAGATTCGTAATTTAAAGGAAGAGTTAGAACAGTTGAAGAAGGGTATTGTTACAGTTCCTCAACTAAAAGATGCTAGCGAAAATGATATTCTGCTTCTAAAACAAAAGTTAGAAGATGGCAAATTCAAACTGCAATCAAGATTGGAACAAGAGGAAGAAGCCAAAGCTGCTTTGTTAGGAAGAATCCAAAGGTTGACAAAGTTGATTTTGGTCTCCACAAAAGCAACACAGCCATCAAGGTTTCCTCACCGACCTGGTCTTAGAAGAAGACATTCCTTTGGGGAAGAGGAGCTTGCATATCTACCATACAAAAGGCGGGACTTGATTTTAGAGGATGAAAACATTGACTTGTTTGTTTCTCCTCTTGAGGGGAATACTGAAACTGCTGATGCGCTGAAGGAGGATAAGAAGACTCGGAAGCATGGACTGTTAAACTGGTTAAAGCTACGGAGGCAAGAAAGGCAGGAGTTCAGTTTTGTGGAAGAATTTAGCGGTAAGTATGTTTGA

Protein Analysis

360

Amino Acids

40.47

Weight (kDa)

8.82

Isoelectric Point (pI)

59.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kinesin PF00225 4 - 171 4e-58 Kinesin motor domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 495
AciI CCGC 2 cut(s) 892, 1068
AclWI GGATC 3 cut(s) 112, 282, 295
AcsI RAATTY 4 cut(s) 112, 516, 698, 1061
AcuI CTGAAG 1 cut(s) 992
AdeI CACNNNGTG 1 cut(s) 410
AfiI CCNNNNNNNGG 2 cut(s) 178, 944
AgsI TTSAA 7 cut(s) 23, 118, 220, 515, 521, 613, 703
AjnI CCWGG 1 cut(s) 829
AluBI AGCT 8 cut(s) 56, 80, 200, 249, 257, 743, 868, 1021
AluI AGCT 8 cut(s) 56, 80, 200, 249, 257, 743, 868, 1021
Alw21I GWGCWC 2 cut(s) 93, 251
Alw26I GTCTC 3 cut(s) 430, 464, 793
AlwI GGATC 3 cut(s) 112, 282, 295
ApeKI GCWGC 3 cut(s) 77, 524, 743
ApoI RAATTY 4 cut(s) 112, 516, 698, 1061
AseI ATTAAT 1 cut(s) 297
Asp700I GAANNNNTTC 1 cut(s) 847
AspLEI GCGC 2 cut(s) 496, 970
AsuC2I CCSGG 1 cut(s) 500
AsuHPI GGTGA 2 cut(s) 206, 815
AsuNHI GCTAGC 1 cut(s) 650
BaeGI GKGCMC 1 cut(s) 14
BamHI GGATCC 1 cut(s) 287
BanII GRGCYC 1 cut(s) 251
BbsI GAAGAC 2 cut(s) 850, 992
Bbv12I GWGCWC 2 cut(s) 93, 251
BbvI GCAGC 3 cut(s) 89, 536, 730
BccI CCATC 3 cut(s) 338, 686, 817
BciT130I CCWGG 1 cut(s) 831
BcnI CCSGG 1 cut(s) 500
BcoDI GTCTC 3 cut(s) 430, 464, 793
BfaI CTAG 2 cut(s) 317, 651
BfmI CTRYAG 1 cut(s) 367
BisI GCNGC 3 cut(s) 78, 525, 744
BlsI GCNGC 3 cut(s) 79, 526, 745
Bme1390I CCNGG 2 cut(s) 500, 831
BmiI GGNNCC 2 cut(s) 137, 289
BmrFI CCNGG 2 cut(s) 500, 831
BmsI GCATC 2 cut(s) 637, 955
BmtI GCTAGC 1 cut(s) 654
BpiI GAAGAC 2 cut(s) 850, 992
BplI GAGNNNNNCTC 2 cut(s) 340, 372
BpmI CTGGAG 1 cut(s) 102
BpuEI CTTGAG 1 cut(s) 962
BpuMI CCSGG 1 cut(s) 500
BsaI GGTCTC 1 cut(s) 793
BsaXI ACNNNNNCTCC 2 cut(s) 430, 460
Bsc4I CCNNNNNNNGG 2 cut(s) 178, 944
Bse1I ACTGG 2 cut(s) 271, 1016
BseBI CCWGG 1 cut(s) 831
BseGI GGATG 2 cut(s) 349, 916
BseLI CCNNNNNNNGG 2 cut(s) 178, 944
BseMII CTCAG 2 cut(s) 139, 234
BseNI ACTGG 2 cut(s) 271, 1016
BseRI GAGGAG 2 cut(s) 878, 927
BseSI GKGCMC 1 cut(s) 14
BseXI GCAGC 3 cut(s) 89, 536, 730
BseYI CCCAGC 1 cut(s) 52
BsgI GTGCAG 1 cut(s) 421
BsiHKAI GWGCWC 2 cut(s) 93, 251
BsiSI CCGG 1 cut(s) 499
BslFI GGGAC 1 cut(s) 908
BslI CCNNNNNNNGG 2 cut(s) 178, 944
BsmAI GTCTC 3 cut(s) 430, 464, 793
BsmBI CGTCTC 1 cut(s) 464
BsmFI GGGAC 1 cut(s) 908
Bso31I GGTCTC 1 cut(s) 793
Bsp1286I GDGCHC 3 cut(s) 14, 93, 251
Bsp143I GATC 3 cut(s) 104, 229, 287
BspACI CCGC 2 cut(s) 892, 1068
BspCNI CTCAG 2 cut(s) 138, 235
BspLI GGNNCC 2 cut(s) 137, 289
BspOI GCTAGC 1 cut(s) 654
BspPI GGATC 3 cut(s) 112, 282, 295
BspQI GCTCTTC 1 cut(s) 81
BspTNI GGTCTC 1 cut(s) 793
BsrI ACTGG 2 cut(s) 271, 1016
BssMI GATC 3 cut(s) 104, 229, 287
Bst2UI CCWGG 1 cut(s) 831
Bst4CI ACNGT 5 cut(s) 325, 442, 609, 631, 1005
Bst6I CTCTTC 4 cut(s) 81, 462, 591, 855
BstC8I GCNNGC 2 cut(s) 652, 870
BstDEI CTNAG 4 cut(s) 125, 243, 407, 836
BstF5I GGATG 2 cut(s) 349, 916
BstHHI GCGC 2 cut(s) 496, 970
BstKTI GATC 3 cut(s) 107, 232, 290
BstMAI GTCTC 3 cut(s) 430, 464, 793
BstMBI GATC 3 cut(s) 104, 229, 287
BstMWI GCNNNNNNNGC 4 cut(s) 500, 743, 1027, 1036
BstNI CCWGG 1 cut(s) 831
BstNSI RCATGY 1 cut(s) 101
BstSCI CCNGG 2 cut(s) 498, 829
BstSFI CTRYAG 1 cut(s) 367
BstSLI GKGCMC 1 cut(s) 14
BstV1I GCAGC 3 cut(s) 89, 536, 730
BstV2I GAAGAC 2 cut(s) 850, 992
BstX2I RGATCY 1 cut(s) 287
BstYI RGATCY 1 cut(s) 287
BtsCI GGATG 2 cut(s) 349, 916
BtsIMutI CAGTG 2 cut(s) 152, 438
Cac8I GCNNGC 2 cut(s) 652, 870
CfoI GCGC 2 cut(s) 496, 970
CseI GACGC 1 cut(s) 481
CsiI ACCWGGT 1 cut(s) 829
CviAII CATG 4 cut(s) 59, 98, 416, 998
DdeI CTNAG 4 cut(s) 125, 243, 407, 836
DpnI GATC 3 cut(s) 106, 231, 289
DpnII GATC 3 cut(s) 104, 229, 287
DraI TTTAAA 1 cut(s) 591
DraIII CACNNNGTG 1 cut(s) 410
Eam1104I CTCTTC 4 cut(s) 81, 462, 591, 855
EarI CTCTTC 4 cut(s) 81, 462, 591, 855
Ecl136II GAGCTC 1 cut(s) 249
Eco24I GRGCYC 1 cut(s) 251
Eco31I GGTCTC 1 cut(s) 793
Eco53kI GAGCTC 1 cut(s) 249
Eco57I CTGAAG 1 cut(s) 992
EcoICRI GAGCTC 1 cut(s) 249
EcoRII CCWGG 1 cut(s) 829
EcoT38I GRGCYC 1 cut(s) 251
Esp3I CGTCTC 1 cut(s) 464
FaeI CATG 4 cut(s) 62, 101, 419, 1001
FalI AAGNNNNNCTT 2 cut(s) 852, 884
FaqI GGGAC 1 cut(s) 908
FatI CATG 4 cut(s) 58, 97, 415, 997
FauI CCCGC 1 cut(s) 885
Fnu4HI GCNGC 3 cut(s) 78, 525, 744
FokI GGATG 2 cut(s) 356, 923
FriOI GRGCYC 1 cut(s) 251
Fsp4HI GCNGC 3 cut(s) 78, 525, 744
FspBI CTAG 2 cut(s) 317, 651
FspI TGCGCA 1 cut(s) 495
GlaI GCGC 2 cut(s) 495, 969
GluI GCNGC 3 cut(s) 78, 525, 744
GsaI CCCAGC 1 cut(s) 56
GsuI CTGGAG 1 cut(s) 102
HapII CCGG 1 cut(s) 499
HgaI GACGC 1 cut(s) 481
HhaI GCGC 2 cut(s) 496, 970
Hin1II CATG 4 cut(s) 62, 101, 419, 1001
Hin6I GCGC 2 cut(s) 494, 968
HinP1I GCGC 2 cut(s) 494, 968
HincII GTYRAC 2 cut(s) 339, 771
HindII GTYRAC 2 cut(s) 339, 771
HinfI GANTC 5 cut(s) 304, 374, 580, 759, 988
HpaII CCGG 1 cut(s) 499
HphI GGTGA 2 cut(s) 206, 815
Hpy166II GTNNAC 2 cut(s) 339, 771
Hpy188I TCNGA 4 cut(s) 244, 275, 466, 993
Hpy188III TCNNGA 4 cut(s) 163, 562, 714, 941
Hpy8I GTNNAC 2 cut(s) 339, 771
HpyAV CCTTC 6 cut(s) 17, 185, 278, 459, 610, 967
HpyCH4III ACNGT 5 cut(s) 325, 442, 609, 631, 1005
HpyCH4V TGCA 3 cut(s) 438, 709, 872
HpyF10VI GCNNNNNNNGC 4 cut(s) 500, 743, 1027, 1036
HpyF3I CTNAG 4 cut(s) 125, 243, 407, 836
Hsp92II CATG 4 cut(s) 62, 101, 419, 1001
HspAI GCGC 2 cut(s) 494, 968
Kzo9I GATC 3 cut(s) 104, 229, 287
LguI GCTCTTC 1 cut(s) 81
LmnI GCTCC 2 cut(s) 135, 865
Lsp1109I GCAGC 3 cut(s) 89, 536, 730
LweI GCATC 2 cut(s) 637, 955
MabI ACCWGGT 1 cut(s) 829
MaeI CTAG 2 cut(s) 317, 651
MaeIII GTNAC 4 cut(s) 66, 442, 552, 625
MalI GATC 3 cut(s) 106, 231, 289
MboI GATC 3 cut(s) 104, 229, 287
MfeI CAATTG 1 cut(s) 215
MflI RGATCY 1 cut(s) 287
MhlI GDGCHC 3 cut(s) 14, 93, 251
MluCI AATT 7 cut(s) 112, 118, 215, 516, 586, 698, 1061
MlyI GAGTC 2 cut(s) 313, 982
MmeI TCCRAC 1 cut(s) 82
MroXI GAANNNNTTC 1 cut(s) 847
MseI TTAA 5 cut(s) 297, 485, 590, 1007, 1016
MslI CAYNNNNRTG 2 cut(s) 96, 420
MspA1I CMGCKG 1 cut(s) 80
MspI CCGG 1 cut(s) 499
MspR9I CCNGG 2 cut(s) 500, 831
MunI CAATTG 1 cut(s) 215
MvaI CCWGG 1 cut(s) 831
MwoI GCNNNNNNNGC 4 cut(s) 500, 743, 1027, 1036
NciI CCSGG 1 cut(s) 500
NdeII GATC 3 cut(s) 104, 229, 287
NheI GCTAGC 1 cut(s) 650
NlaIII CATG 4 cut(s) 62, 101, 419, 1001
NlaIV GGNNCC 2 cut(s) 137, 289
NmuCI GTSAC 2 cut(s) 66, 552
NsbI TGCGCA 1 cut(s) 495
NspI RCATGY 1 cut(s) 101
PciSI GCTCTTC 1 cut(s) 81
PdmI GAANNNNTTC 1 cut(s) 847
PfeI GAWTC 3 cut(s) 374, 580, 759
PflFI GACNNNGTC 1 cut(s) 831
PkrI GCNGC 3 cut(s) 79, 526, 745
PleI GAGTC 2 cut(s) 312, 982
PpsI GAGTC 2 cut(s) 312, 982
PshBI ATTAAT 1 cut(s) 297
Psp124BI GAGCTC 1 cut(s) 251
Psp6I CCWGG 1 cut(s) 829
PspFI CCCAGC 1 cut(s) 52
PspGI CCWGG 1 cut(s) 829
PspN4I GGNNCC 2 cut(s) 137, 289
PsuI RGATCY 1 cut(s) 287
PsyI GACNNNGTC 1 cut(s) 831
PvuII CAGCTG 1 cut(s) 80
RseI CAYNNNNRTG 2 cut(s) 96, 420
SacI GAGCTC 1 cut(s) 251
SapI GCTCTTC 1 cut(s) 81
SaqAI TTAA 5 cut(s) 297, 485, 590, 1007, 1016
SatI GCNGC 3 cut(s) 78, 525, 744
Sau3AI GATC 3 cut(s) 104, 229, 287
SchI GAGTC 2 cut(s) 313, 982
ScrFI CCNGG 2 cut(s) 500, 831
SduI GDGCHC 3 cut(s) 14, 93, 251
SexAI ACCWGGT 1 cut(s) 829
SfaNI GCATC 2 cut(s) 637, 955
SfcI CTRYAG 1 cut(s) 367
SmiMI CAYNNNNRTG 2 cut(s) 96, 420
SmlI CTYRAG 1 cut(s) 941
SmoI CTYRAG 1 cut(s) 941
Sse9I AATT 7 cut(s) 112, 118, 215, 516, 586, 698, 1061
SsiI CCGC 2 cut(s) 892, 1068
SspMI CTAG 2 cut(s) 317, 651
SstI GAGCTC 1 cut(s) 251
StyD4I CCNGG 2 cut(s) 498, 829
TaaI ACNGT 5 cut(s) 325, 442, 609, 631, 1005
TaqI TCGA 1 cut(s) 162
TasI AATT 7 cut(s) 112, 118, 215, 516, 586, 698, 1061
TfiI GAWTC 3 cut(s) 374, 580, 759
Tru1I TTAA 5 cut(s) 297, 485, 590, 1007, 1016
Tru9I TTAA 5 cut(s) 297, 485, 590, 1007, 1016
TscAI CASTG 2 cut(s) 159, 445
TseFI GTSAC 2 cut(s) 66, 552
TseI GCWGC 3 cut(s) 77, 524, 743
Tsp45I GTSAC 2 cut(s) 66, 552
TspDTI ATGAA 2 cut(s) 441, 927
TspGWI ACGGA 2 cut(s) 356, 1039
TspRI CASTG 2 cut(s) 159, 445
Tth111I GACNNNGTC 1 cut(s) 831
VspI ATTAAT 1 cut(s) 297
XapI RAATTY 4 cut(s) 112, 516, 698, 1061
XceI RCATGY 1 cut(s) 101
XmnI GAANNNNTTC 1 cut(s) 847
XspI CTAG 2 cut(s) 317, 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.