pycom12g13530

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0029259
Physical Location & Seq
Reverse (-)
16030195 .. 16031106
912 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g13530.2

Sequence Viewer

Length: 849 bp
ATGAATCCATCTGAAATGCTTGTTACCTCTGCTGATTCCCGGATTCGAATTTTGGATGGCACAAACGTGACTCATAAGTTTAGAGGTATGCAGAGCTCATCCAATGCTCAGAGTTTCCGAAATACAAGCAGTCAAATTGCAGCTTCATTCACTCCAAACGGAAAGTATGTTGTATGTGCTAGTGAAGACTCCCATGTGTATGTTTGGAAGCGCGAAGAACCCCGAATCTCAGCCACGGGTAAAAAAAGCATCATCAGTACCAATTCCCACGAGCATTTTCAGTGCAAAGATGTTTCAGCAGCAATACCATGGCCGGGCACCATAAAGGGCGACCCTCCACCTGTCCCCGTGCAGCATTCCAAGAGAAACTCAAAACGCTCAACGCAACAGCCGCAATCCGGCAATGCATCCCCTACTCAAGAAGATGCAGGTGTAAGCAAGAGAATGCTACCGCCTCTTCCGAAGAAAAACAACAAAGACAACAACACCACAACCAATAAGGGAGATGCATCAGATCAAACACCTTCAACTCCTCCGCCGGAAGATCAAGATCCTGCTCAAATCTCTCGAACAGAATCCGGGATTGGAGAGTCGTTTAATTCAGACCCTTCCTCTATTAGGTATGGTGATTCAAGTTCTATGTCTGCTGGTAGTGCCTCCACATCATCTTGGTCTTCCTCTTGGTCATGGCTCGACAATATAGGCAACAGCCATGCTAGCCAAACAACACAAGCAACAGCTTGGGGTTTGGTAATTGTGACAGCCACTCTGGAAGGCGAAATCAGAGCATACCAAAATTTCGGGCTGCCGCGAAAGATGCAGAACAATATTTTTGGAGGCCCTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

283

Amino Acids

30.44

Weight (kDa)

8.83

Isoelectric Point (pI)

53.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029259)

Species Orthologous Gene IDs
pyrus_communis pycom04g11400 pycom12g13530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 419
Acc36I ACCTGC 1 cut(s) 419
AccB1I GGYRCC 1 cut(s) 317
AccII CGCG 2 cut(s) 213, 811
AciI CCGC 4 cut(s) 392, 452, 536, 809
AclWI GGATC 1 cut(s) 545
AcoI YGGCCR 1 cut(s) 311
AcsI RAATTY 2 cut(s) 48, 796
AfaI GTAC 1 cut(s) 259
AfiI CCNNNNNNNGG 3 cut(s) 314, 398, 618
AgsI TTSAA 2 cut(s) 528, 633
AjuI GAANNNNNNNTTGG 2 cut(s) 567, 599
AleI CACNNNNGTG 1 cut(s) 65
AluBI AGCT 3 cut(s) 96, 143, 740
AluI AGCT 3 cut(s) 96, 143, 740
Alw21I GWGCWC 1 cut(s) 98
AlwI GGATC 1 cut(s) 545
AoxI GGCC 2 cut(s) 311, 838
ApeKI GCWGC 4 cut(s) 140, 299, 352, 805
ApoI RAATTY 2 cut(s) 48, 796
AspLEI GCGC 1 cut(s) 213
AspS9I GGNCC 1 cut(s) 839
AsuC2I CCSGG 3 cut(s) 40, 315, 580
AsuHPI GGTGA 1 cut(s) 638
AsuII TTCGAA 1 cut(s) 46
AsuNHI GCTAGC 1 cut(s) 716
BaeGI GKGCMC 1 cut(s) 320
BanI GGYRCC 1 cut(s) 317
BanII GRGCYC 1 cut(s) 98
BauI CACGAG 1 cut(s) 269
BbsI GAAGAC 2 cut(s) 192, 666
Bbv12I GWGCWC 1 cut(s) 98
BbvI GCAGC 4 cut(s) 152, 311, 364, 792
BccI CCATC 2 cut(s) 16, 50
BcnI CCSGG 3 cut(s) 40, 315, 580
BfaI CTAG 2 cut(s) 180, 717
BfuAI ACCTGC 1 cut(s) 419
BisI GCNGC 6 cut(s) 141, 300, 353, 392, 806, 809
BlsI GCNGC 6 cut(s) 142, 301, 354, 393, 807, 810
Bme1390I CCNGG 3 cut(s) 40, 315, 580
BmgT120I GGNCC 1 cut(s) 839
BmiI GGNNCC 1 cut(s) 319
BmrFI CCNGG 3 cut(s) 40, 315, 580
BmsI GCATC 6 cut(s) 258, 415, 416, 496, 518, 807
BmtI GCTAGC 1 cut(s) 720
BpiI GAAGAC 2 cut(s) 192, 666
Bpu14I TTCGAA 1 cut(s) 46
BpuEI CTTGAG 1 cut(s) 402
BpuMI CCSGG 3 cut(s) 40, 315, 580
BsaBI GATNNNNATC 1 cut(s) 549
BsaJI CCNNGG 2 cut(s) 234, 308
Bsc4I CCNNNNNNNGG 3 cut(s) 314, 398, 618
Bse3DI GCAATG 1 cut(s) 409
Bse8I GATNNNNATC 1 cut(s) 549
BseDI CCNNGG 2 cut(s) 234, 308
BseGI GGATG 3 cut(s) 61, 98, 407
BseJI GATNNNNATC 1 cut(s) 549
BseLI CCNNNNNNNGG 3 cut(s) 314, 398, 618
BseMI GCAATG 1 cut(s) 409
BseMII CTCAG 2 cut(s) 122, 243
BseRI GAGGAG 1 cut(s) 522
BseSI GKGCMC 1 cut(s) 320
BseXI GCAGC 4 cut(s) 152, 311, 364, 792
BsgI GTGCAG 1 cut(s) 371
Bsh1236I CGCG 2 cut(s) 213, 811
BshFI GGCC 2 cut(s) 313, 840
BshNI GGYRCC 1 cut(s) 317
BsiHKAI GWGCWC 1 cut(s) 98
BsiSI CCGG 5 cut(s) 40, 314, 399, 539, 579
BslFI GGGAC 1 cut(s) 329
BslI CCNNNNNNNGG 3 cut(s) 314, 398, 618
BsmFI GGGAC 1 cut(s) 329
BsmI GAATGC 2 cut(s) 355, 450
BsnI GGCC 2 cut(s) 313, 840
Bsp119I TTCGAA 1 cut(s) 46
Bsp1286I GDGCHC 2 cut(s) 98, 320
Bsp143I GATC 3 cut(s) 514, 544, 550
Bsp19I CCATGG 1 cut(s) 308
BspACI CCGC 4 cut(s) 392, 452, 536, 809
BspANI GGCC 2 cut(s) 313, 840
BspCNI CTCAG 2 cut(s) 121, 242
BspFNI CGCG 2 cut(s) 213, 811
BspLI GGNNCC 1 cut(s) 319
BspMI ACCTGC 1 cut(s) 419
BspOI GCTAGC 1 cut(s) 720
BspPI GGATC 1 cut(s) 545
BspT104I TTCGAA 1 cut(s) 46
BspT107I GGYRCC 1 cut(s) 317
BsrDI GCAATG 1 cut(s) 409
BssECI CCNNGG 2 cut(s) 234, 308
BssMI GATC 3 cut(s) 514, 544, 550
BssSI CACGAG 1 cut(s) 269
BssT1I CCWWGG 1 cut(s) 308
Bst2BI CACGAG 1 cut(s) 269
Bst6I CTCTTC 1 cut(s) 462
BstBI TTCGAA 1 cut(s) 46
BstC8I GCNNGC 1 cut(s) 718
BstDEI CTNAG 2 cut(s) 108, 229
BstDSI CCRYGG 2 cut(s) 234, 308
BstENI CCTNNNNNAGG 1 cut(s) 616
BstF5I GGATG 3 cut(s) 61, 98, 407
BstFNI CGCG 2 cut(s) 213, 811
BstHHI GCGC 1 cut(s) 213
BstKTI GATC 3 cut(s) 517, 547, 553
BstMBI GATC 3 cut(s) 514, 544, 550
BstMWI GCNNNNNNNGC 4 cut(s) 391, 653, 717, 817
BstSCI CCNGG 3 cut(s) 38, 313, 578
BstSLI GKGCMC 1 cut(s) 320
BstUI CGCG 2 cut(s) 213, 811
BstV1I GCAGC 4 cut(s) 152, 311, 364, 792
BstV2I GAAGAC 2 cut(s) 192, 666
BstX2I RGATCY 1 cut(s) 550
BstYI RGATCY 1 cut(s) 550
BsuRI GGCC 2 cut(s) 313, 840
BtgI CCRYGG 2 cut(s) 234, 308
BtsCI GGATG 3 cut(s) 61, 98, 407
BtsIMutI CAGTG 1 cut(s) 287
BveI ACCTGC 1 cut(s) 419
Cac8I GCNNGC 1 cut(s) 718
CfoI GCGC 1 cut(s) 213
Cfr13I GGNCC 1 cut(s) 839
Csp6I GTAC 1 cut(s) 258
CviAII CATG 4 cut(s) 194, 309, 687, 713
CviQI GTAC 1 cut(s) 258
DdeI CTNAG 2 cut(s) 108, 229
DpnI GATC 3 cut(s) 516, 546, 552
DpnII GATC 3 cut(s) 514, 544, 550
EaeI YGGCCR 1 cut(s) 311
Eam1104I CTCTTC 1 cut(s) 462
EarI CTCTTC 1 cut(s) 462
EciI GGCGGA 1 cut(s) 525
Ecl136II GAGCTC 1 cut(s) 96
Eco130I CCWWGG 1 cut(s) 308
Eco24I GRGCYC 1 cut(s) 98
Eco53kI GAGCTC 1 cut(s) 96
EcoICRI GAGCTC 1 cut(s) 96
EcoNI CCTNNNNNAGG 1 cut(s) 616
EcoO109I RGGNCCY 1 cut(s) 839
EcoT14I CCWWGG 1 cut(s) 308
EcoT22I ATGCAT 2 cut(s) 409, 511
EcoT38I GRGCYC 1 cut(s) 98
ErhI CCWWGG 1 cut(s) 308
FaeI CATG 4 cut(s) 197, 312, 690, 716
FaqI GGGAC 1 cut(s) 329
FatI CATG 4 cut(s) 193, 308, 686, 712
Fnu4HI GCNGC 6 cut(s) 141, 300, 353, 392, 806, 809
FokI GGATG 3 cut(s) 68, 85, 394
FriOI GRGCYC 1 cut(s) 98
Fsp4HI GCNGC 6 cut(s) 141, 300, 353, 392, 806, 809
FspBI CTAG 2 cut(s) 180, 717
GlaI GCGC 1 cut(s) 212
GluI GCNGC 6 cut(s) 141, 300, 353, 392, 806, 809
HaeIII GGCC 2 cut(s) 313, 840
HapII CCGG 5 cut(s) 40, 314, 399, 539, 579
HhaI GCGC 1 cut(s) 213
Hin1II CATG 4 cut(s) 197, 312, 690, 716
Hin6I GCGC 1 cut(s) 211
HinP1I GCGC 1 cut(s) 211
HinfI GANTC 9 cut(s) 4, 35, 43, 70, 188, 225, 575, 590, 629
HpaII CCGG 5 cut(s) 40, 314, 399, 539, 579
HphI GGTGA 1 cut(s) 638
Hpy188I TCNGA 7 cut(s) 13, 111, 119, 462, 514, 604, 785
Hpy188III TCNNGA 4 cut(s) 419, 548, 567, 770
HpyAV CCTTC 3 cut(s) 534, 618, 767
HpyCH4IV ACGT 1 cut(s) 66
HpyCH4V TGCA 8 cut(s) 91, 140, 285, 352, 407, 428, 509, 820
HpyF10VI GCNNNNNNNGC 4 cut(s) 391, 653, 717, 817
HpyF3I CTNAG 2 cut(s) 108, 229
HpySE526I ACGT 1 cut(s) 66
Hsp92II CATG 4 cut(s) 197, 312, 690, 716
HspAI GCGC 1 cut(s) 211
Kzo9I GATC 3 cut(s) 514, 544, 550
Lsp1109I GCAGC 4 cut(s) 152, 311, 364, 792
LweI GCATC 6 cut(s) 258, 415, 416, 496, 518, 807
MaeI CTAG 2 cut(s) 180, 717
MaeII ACGT 1 cut(s) 66
MaeIII GTNAC 3 cut(s) 22, 67, 757
MalI GATC 3 cut(s) 516, 546, 552
MboI GATC 3 cut(s) 514, 544, 550
MboII GAAGA 7 cut(s) 197, 227, 434, 449, 475, 554, 666
MflI RGATCY 1 cut(s) 550
MhlI GDGCHC 2 cut(s) 98, 320
MluCI AATT 6 cut(s) 48, 135, 262, 598, 753, 796
MlyI GAGTC 3 cut(s) 64, 182, 599
MnlI CCTC 9 cut(s) 37, 77, 345, 465, 543, 622, 667, 688, 830
Mph1103I ATGCAT 2 cut(s) 409, 511
MseI TTAA 2 cut(s) 597, 847
MslI CAYNNNNRTG 2 cut(s) 65, 198
MspI CCGG 5 cut(s) 40, 314, 399, 539, 579
MspR9I CCNGG 3 cut(s) 40, 315, 580
Mva1269I GAATGC 2 cut(s) 355, 450
MvnI CGCG 2 cut(s) 213, 811
MwoI GCNNNNNNNGC 4 cut(s) 391, 653, 717, 817
NciI CCSGG 3 cut(s) 40, 315, 580
NcoI CCATGG 1 cut(s) 308
NdeII GATC 3 cut(s) 514, 544, 550
NheI GCTAGC 1 cut(s) 716
NlaIII CATG 4 cut(s) 197, 312, 690, 716
NlaIV GGNNCC 1 cut(s) 319
NmuCI GTSAC 2 cut(s) 67, 757
NsiI ATGCAT 2 cut(s) 409, 511
NspV TTCGAA 1 cut(s) 46
OliI CACNNNNGTG 1 cut(s) 65
PaqCI CACCTGC 1 cut(s) 419
PctI GAATGC 2 cut(s) 355, 450
PfeI GAWTC 6 cut(s) 4, 35, 43, 225, 575, 629
PfoI TCCNGGA 2 cut(s) 38, 578
PkrI GCNGC 6 cut(s) 142, 301, 354, 393, 807, 810
PleI GAGTC 3 cut(s) 64, 182, 598
PpsI GAGTC 3 cut(s) 64, 182, 598
Psp124BI GAGCTC 1 cut(s) 98
PspN4I GGNNCC 1 cut(s) 319
PspPI GGNCC 1 cut(s) 839
PsuI RGATCY 1 cut(s) 550
RsaI GTAC 1 cut(s) 259
RsaNI GTAC 1 cut(s) 258
RseI CAYNNNNRTG 2 cut(s) 65, 198
SacI GAGCTC 1 cut(s) 98
SaqAI TTAA 2 cut(s) 597, 847
SatI GCNGC 6 cut(s) 141, 300, 353, 392, 806, 809
Sau3AI GATC 3 cut(s) 514, 544, 550
Sau96I GGNCC 1 cut(s) 839
SchI GAGTC 3 cut(s) 64, 182, 599
ScrFI CCNGG 3 cut(s) 40, 315, 580
SduI GDGCHC 2 cut(s) 98, 320
SfaNI GCATC 6 cut(s) 258, 415, 416, 496, 518, 807
SfuI TTCGAA 1 cut(s) 46
SmiMI CAYNNNNRTG 2 cut(s) 65, 198
SmlI CTYRAG 1 cut(s) 417
SmoI CTYRAG 1 cut(s) 417
Sse9I AATT 6 cut(s) 48, 135, 262, 598, 753, 796
SsiI CCGC 4 cut(s) 392, 452, 536, 809
SspI AATATT 1 cut(s) 829
SspMI CTAG 2 cut(s) 180, 717
SstI GAGCTC 1 cut(s) 98
StyD4I CCNGG 3 cut(s) 38, 313, 578
StyI CCWWGG 1 cut(s) 308
TaiI ACGT 1 cut(s) 69
TaqI TCGA 3 cut(s) 46, 568, 693
TasI AATT 6 cut(s) 48, 135, 262, 598, 753, 796
TauI GCSGC 2 cut(s) 394, 811
TfiI GAWTC 6 cut(s) 4, 35, 43, 225, 575, 629
Tru1I TTAA 2 cut(s) 597, 847
Tru9I TTAA 2 cut(s) 597, 847
TscAI CASTG 1 cut(s) 287
TseFI GTSAC 2 cut(s) 67, 757
TseI GCWGC 4 cut(s) 140, 299, 352, 805
Tsp45I GTSAC 2 cut(s) 67, 757
TspDTI ATGAA 2 cut(s) 17, 135
TspGWI ACGGA 1 cut(s) 174
TspRI CASTG 1 cut(s) 287
XagI CCTNNNNNAGG 1 cut(s) 616
XapI RAATTY 2 cut(s) 48, 796
XspI CTAG 2 cut(s) 180, 717
Zsp2I ATGCAT 2 cut(s) 409, 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.