Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0019572

Physical Location & Seq

Genomic Coordinates Forward (+)

17476042 .. 17479536

Gene Structure 3495 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2319 bp

ATGATGTTCATAGTTTTTCTGCTGTTCTCGCTGCACAAAGTCGAAGCAGCGTCTTCGTCTGCTTCTTCTTCCTCGGCTTCGAATTATCTGATCGGCCTCGGAAGCCATGACATAACAGGGCCTGCTGCCGATGTGAACATGATGGGATACGCAAATGCAGAGCAGATTGCATCTGGGATTCACTTCCGGCTTCGAGCTCGCGCGTTTATTGTGGCAGAACAACAACAGGGGAATCGTGTGGTGTTTGTGAATCTTGATGCATGCATGGGCTCCCAAATTGTGACAATTAAAGTGCTTGAGAGATTGAAAGCAAGGTATGGGGACCTTTACACAGAGAAGAATGTGGCAATTAGCGGGATTCACACTCATGCAGGGCCTGGGGGCTACCTTCAGTATGTTGTGTATATTGTGACTTCTCTTGGGTTTGTAAGACAATCATTTGATGTTCTTGTTGATGGCATTGAGAAAACCATTATTCAAGCTCACGAAAATCTCCGTCCCGGTTCAATTTTTGTGAATAAAGGAGAGCTCTTAGATGCTGGTGTTAACCGCAGTCCAAGCGCATATCTCAACAATCCTGCAGACGAGAGGAGAAAATACAAGTATGATGTTGATAAAGATATGACACTCATAAAATTTGAAGATGACGAGTGGGGCGCTATAGGTAGCTTTAACTGGTTTGCAACTCATGGAACTTCCATGAGTCGTACAAACTCATTGATAAGCGGAGATAACAAAGGAGCTGCAGCACGATTCATGGAGGACTGGTTTGAACAGAAAGGTGTTAAAGGTTTCGATGGCCTATTTGCCAATAGATCAGATACTGGCAGAATCCCCAGAAGAGTCTCAACCCTAGTTCCTAACGATGATGCGAAAGGCAATGAGTTGATGAGACTCGCCGCCTCCTTTCAACCTTATAAAGGACAACCTGTGACAAGGCTTTCAAGTTCTGCAAGACGGGTTAGGAATGCTTTGAGGAAGGCGGAAAAGCCTGAATTTGTATCTGCATTCTGTCAATCAAATTGTGGCGATGTAAGCCCAAATGTTCTTGGTGCATTCTGCACAGACACAGGACTTCCGTGTGATTTCTATCACAGTACCTGCAATGGCAGAAATGAATTGTGCTATGGCCGGGGACCAGGTTCCGATGAATTTGAGAGTACTCGCATAATTGGTGAAAGGCAATTCAGAAAAGCTGTGGAACTGTTTGACAAAGCAACTGAGAAGCTCAAAGGGAAGATTGGATACCAACATGCATACATAGATTTTTCCAGCCTTGAGGTCTCAGTTCCGAAAGTTGGAGGGGCTGAGGTAGTGAAAACGTGCCCCGCTGCCATGGGGTTTGCCTTTGCTGCAGGAACCACTGATGGCCCTGGAGCTTTCGATTTCAAGCAGGGCGATGACCAGGGTAATGCATTCTGGAGATTGGTAAGGAACGTGCTGAAGGCACCGGACCAGGAGCAAATCAATTGCCAGTGTCCGAAGCCTATTCTGCTTGACACTGGGGAGATGAAGGAACCATATGATTGGGCGCCCTCAATACTACCGGTTCAAATTTTACGAATTGGACAGCTTGTCATTCTCTGTGTACCTGGAGAATTTACAACCATGGCTGGAAGGCGCCTCCGCGATGCTGTCAAAGGGGTCCTTATCTCTGGAGGTAGGAAAGAATTTGACAACAATGTCCATATAGTCATTGCAGGGCTAACAAATACATATTCACAGTATGTTACCACCTTCGAGGAGTACAAAGTGCAGAGATACGAGGGAGCCTCCACGCTTTACGGACCACACACACTTGAAGCGTACATCCAAGAATTCAAGAAACTAGCTGCAGCTCTCGTCAATGGCCAAACTGTCGAACCAGGTCCACAGCCACCAGACCTCCTCGACAAGCAAATCAGCTTACTAGCACCAGTTGTCCTGGACGCCACCCCTCTTGGTGTTAATTTTGGGGATGTTAAAACCGACGTAACTCTAGATTCCACCTTCAAGAGAGGCAACACGGTTGCAGTCACCTTCTGGTCTGCTTGCCCAAGGAATGACCTCCTGACAGAAGGCACATTTGCATTGGTTGAGATTCTACAGAACGACAAAAAGACATGGGTTCCGGCCTATGATGACGATGATTTTTGCTTGAAGTTTAAGTGGTCACGGCCTGCAAAACTTAGCCCTCAGAGCTACGCAACTATTGAATGGAGGATACCGGAAAATGCAGCTTTCGGTGTCTACAGGATTAGCCATTTTGGTGCCTCTAAGAATTTACTCGGGTCCATTCGCCATTTCACAGGTTCTTCAAGTGCTTTTGTAGTAGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0005911 GO:0009506 GO:0012505 GO:0030054 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0055044

KEGG Pathways

Metabolic & Signaling

ko00600 ko01100 ko04071 map00600 map01100 map04071

Pfam Domains

Protein Families

Ceramidase_alk Ceramidse_alk_C

Protein Analysis

773

Amino Acids

84.94

Weight (kDa)

6.9

Isoelectric Point (pI)

33.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ceramidase_alk	PF04734	29 - 605	4.6e-229	Neutral/alkaline non-lysosomal ceramidase, N-terminal
Ceramidse_alk_C	PF17048	607 - 771	8.9e-56	Neutral/alkaline non-lysosomal ceramidase, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0019572)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38010 AT2G38010 AT2G38010
malus_domestica	MD12G1158800.v1.1
prunus_persica	Prupe.6G269200_v2.0.a1 Prupe.6G269200_v2.0.a1
pyrus_communis	pycom12g15070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	918
AasI	GACNNNNNNGTC	1 cut(s)	1682
Acc36I	ACCTGC	1 cut(s)	1109
AccB1I	GGYRCC	4 cut(s)	1447, 1531, 1620, 2249
AccI	GTMKAC	1 cut(s)	2229
AccII	CGCG	3 cut(s)	201, 203, 1629
AciI	CCGC	7 cut(s)	354, 550, 726, 900, 983, 1329, 1627
AcoI	YGGCCR	2 cut(s)	1129, 1849
AcsI	RAATTY	8 cut(s)	635, 995, 1151, 1554, 1598, 1670, 1817, 2260
AcuI	CTGAAG	2 cut(s)	374, 1463
AcyI	GRCGYC	3 cut(s)	1532, 1621, 1929
AfaI	GTAC	6 cut(s)	709, 1099, 1162, 1590, 1748, 1808
AfiI	CCNNNNNNNGG	2 cut(s)	920, 1298
AgeI	ACCGGT	1 cut(s)	1546
AhdI	GACNNNNNGTC	1 cut(s)	1574
AjnI	CCWGG	8 cut(s)	376, 1138, 1372, 1404, 1455, 1591, 1864, 1923
Alw21I	GWGCWC	2 cut(s)	199, 531
Alw26I	GTCTC	3 cut(s)	850, 886, 1288
AlwNI	CAGNNNCTG	4 cut(s)	122, 377, 824, 1101
Ama87I	CYCGRG	1 cut(s)	2267
AoxI	GGCC	9 cut(s)	94, 119, 374, 799, 1129, 1369, 1849, 2112, 2156
ApoI	RAATTY	8 cut(s)	635, 995, 1151, 1554, 1598, 1670, 1817, 2260
AsiGI	ACCGGT	1 cut(s)	1546
AspLEI	GCGC	5 cut(s)	203, 563, 659, 1534, 1623
AsuC2I	CCSGG	2 cut(s)	501, 1133
AsuHPI	GGTGA	2 cut(s)	1187, 2008
AsuII	TTCGAA	1 cut(s)	80
AvaI	CYCGRG	1 cut(s)	2267
AvaII	GGWCC	7 cut(s)	322, 1136, 1453, 1645, 1787, 1868, 2271
BaeGI	GKGCMC	1 cut(s)	1328
BalI	TGGCCA	1 cut(s)	1851
BanI	GGYRCC	4 cut(s)	1447, 1531, 1620, 2249
BanII	GRGCYC	3 cut(s)	199, 272, 531
BarI	GAAGNNNNNNTAC	2 cut(s)	1229, 1261
BbsI	GAAGAC	1 cut(s)	45
Bbv12I	GWGCWC	2 cut(s)	199, 531
BbvCI	CCTCAGC	1 cut(s)	1308
BccI	CCATC	4 cut(s)	136, 449, 791, 1361
BceAI	ACGGC	1 cut(s)	2171
BcgI	CGANNNNNNTGC	2 cut(s)	36, 70
BciT130I	CCWGG	8 cut(s)	378, 1140, 1374, 1406, 1457, 1593, 1866, 1925
BciVI	GTATCC	3 cut(s)	140, 1238, 2196
BcnI	CCSGG	2 cut(s)	501, 1133
BcoDI	GTCTC	3 cut(s)	850, 886, 1288
BfaI	CTAG	4 cut(s)	854, 1829, 1910, 1979
BfmI	CTRYAG	7 cut(s)	579, 660, 744, 1353, 1833, 2084, 2230
BfoI	RGCGCY	3 cut(s)	660, 1535, 1624
BfuAI	ACCTGC	1 cut(s)	1109
BfuI	GTATCC	3 cut(s)	140, 1238, 2196
BmcAI	AGTACT	1 cut(s)	1162
Bme18I	GGWCC	7 cut(s)	322, 1136, 1453, 1645, 1787, 1868, 2271
BmeRI	GACNNNNNGTC	1 cut(s)	1574
BmeT110I	CYCGRG	1 cut(s)	2267
BmrI	ACTGGG	1 cut(s)	1512
BmsI	GCATC	5 cut(s)	179, 247, 526, 859, 1621
BmuI	ACTGGG	1 cut(s)	1512
BpiI	GAAGAC	1 cut(s)	45
BplI	GAGNNNNNCTC	2 cut(s)	1757, 1789
BpmI	CTGGAG	4 cut(s)	1395, 1441, 1614, 1677
Bpu10I	CCTNAGC	1 cut(s)	1308
Bpu14I	TTCGAA	1 cut(s)	80
BpuEI	CTTGAG	2 cut(s)	317, 1298
BpuMI	CCSGG	2 cut(s)	501, 1133
BsaBI	GATNNNNATC	1 cut(s)	1089
BsaHI	GRCGYC	3 cut(s)	1532, 1621, 1929
BsaI	GGTCTC	1 cut(s)	1288
BsaJI	CCNNGG	9 cut(s)	72, 97, 377, 1132, 1335, 1372, 1405, 1608, 2036
BsaWI	WCCGGW	3 cut(s)	1450, 1546, 2206
BsaXI	ACNNNNNCTCC	4 cut(s)	752, 782, 1869, 1899
Bsc4I	CCNNNNNNNGG	2 cut(s)	920, 1298
Bse118I	RCCGGY	1 cut(s)	1546
Bse1I	ACTGG	6 cut(s)	680, 770, 829, 1474, 1507, 1916
Bse3DI	GCAATG	3 cut(s)	886, 1111, 1695
Bse8I	GATNNNNATC	1 cut(s)	1089
BseBI	CCWGG	8 cut(s)	378, 1140, 1374, 1406, 1457, 1593, 1866, 1925
BseDI	CCNNGG	9 cut(s)	72, 97, 377, 1132, 1335, 1372, 1405, 1608, 2036
BseGI	GGATG	2 cut(s)	1809, 1963
BseJI	GATNNNNATC	1 cut(s)	1089
BseLI	CCNNNNNNNGG	2 cut(s)	920, 1298
BseMI	GCAATG	3 cut(s)	886, 1111, 1695
BseMII	CTCAG	4 cut(s)	1212, 1299, 1299, 2189
BseNI	ACTGG	6 cut(s)	680, 770, 829, 1474, 1507, 1916
BseRI	GAGGAG	3 cut(s)	604, 1757, 1877
BseSI	GKGCMC	1 cut(s)	1328
BsgI	GTGCAG	3 cut(s)	17, 1045, 1775
Bsh1236I	CGCG	3 cut(s)	201, 203, 1629
BshFI	GGCC	9 cut(s)	96, 121, 376, 801, 1131, 1371, 1851, 2114, 2158
BshNI	GGYRCC	4 cut(s)	1447, 1531, 1620, 2249
BshTI	ACCGGT	1 cut(s)	1546
BsiHKAI	GWGCWC	2 cut(s)	199, 531
BsiHKCI	CYCGRG	1 cut(s)	2267
BsiSI	CCGG	7 cut(s)	187, 501, 1132, 1451, 1547, 2111, 2207
BslFI	GGGAC	3 cut(s)	335, 483, 1149
BslI	CCNNNNNNNGG	2 cut(s)	920, 1298
BsmAI	GTCTC	3 cut(s)	850, 886, 1288
BsmFI	GGGAC	3 cut(s)	335, 483, 1149
BsmI	GAATGC	4 cut(s)	973, 1007, 1055, 1415
BsnI	GGCC	9 cut(s)	96, 121, 376, 801, 1131, 1371, 1851, 2114, 2158
Bso31I	GGTCTC	1 cut(s)	1288
BsoBI	CYCGRG	1 cut(s)	2267
Bsp119I	TTCGAA	1 cut(s)	80
Bsp1286I	GDGCHC	4 cut(s)	199, 272, 531, 1328
Bsp143I	GATC	2 cut(s)	90, 815
Bsp19I	CCATGG	2 cut(s)	1335, 1608
BspACI	CCGC	7 cut(s)	354, 550, 726, 900, 983, 1329, 1627
BspANI	GGCC	9 cut(s)	96, 121, 376, 801, 1131, 1371, 1851, 2114, 2158
BspCNI	CTCAG	4 cut(s)	1213, 1298, 1300, 2188
BspFNI	CGCG	3 cut(s)	201, 203, 1629
BspMAI	CTGCAG	4 cut(s)	583, 748, 1357, 1837
BspMI	ACCTGC	1 cut(s)	1109
BspT104I	TTCGAA	1 cut(s)	80
BspT107I	GGYRCC	4 cut(s)	1447, 1531, 1620, 2249
BspTNI	GGTCTC	1 cut(s)	1288
BsrDI	GCAATG	3 cut(s)	886, 1111, 1695
BsrFI	RCCGGY	1 cut(s)	1546
BsrI	ACTGG	6 cut(s)	680, 770, 829, 1474, 1507, 1916
BssAI	RCCGGY	1 cut(s)	1546
BssECI	CCNNGG	9 cut(s)	72, 97, 377, 1132, 1335, 1372, 1405, 1608, 2036
BssMI	GATC	2 cut(s)	90, 815
BssNI	GRCGYC	3 cut(s)	1532, 1621, 1929
BssT1I	CCWWGG	3 cut(s)	1335, 1608, 2036
Bst2UI	CCWGG	8 cut(s)	378, 1140, 1374, 1406, 1457, 1593, 1866, 1925
Bst4CI	ACNGT	5 cut(s)	1097, 1206, 1725, 1858, 2008
Bst6I	CTCTTC	1 cut(s)	835
BstACI	GRCGYC	3 cut(s)	1532, 1621, 1929
BstBI	TTCGAA	1 cut(s)	80
BstC8I	GCNNGC	5 cut(s)	123, 199, 262, 2032, 2160
BstDEI	CTNAG	8 cut(s)	532, 1221, 1285, 1308, 2168, 2175, 2256, 2316
BstDSI	CCRYGG	2 cut(s)	1335, 1608
BstENI	CCTNNNNNAGG	1 cut(s)	918
BstF5I	GGATG	2 cut(s)	1809, 1963
BstFNI	CGCG	3 cut(s)	201, 203, 1629
BstH2I	RGCGCY	3 cut(s)	660, 1535, 1624
BstHHI	GCGC	5 cut(s)	203, 563, 659, 1534, 1623
BstKTI	GATC	2 cut(s)	93, 818
BstMAI	GTCTC	3 cut(s)	850, 886, 1288
BstMBI	GATC	2 cut(s)	90, 815
BstMWI	GCNNNNNNNGC	7 cut(s)	28, 102, 558, 1035, 1352, 1492, 2178
BstNI	CCWGG	8 cut(s)	378, 1140, 1374, 1406, 1457, 1593, 1866, 1925
BstNSI	RCATGY	2 cut(s)	264, 1256
BstSFI	CTRYAG	7 cut(s)	579, 660, 744, 1353, 1833, 2084, 2230
BstSLI	GKGCMC	1 cut(s)	1328
BstUI	CGCG	3 cut(s)	201, 203, 1629
BstV2I	GAAGAC	1 cut(s)	45
BstXI	CCANNNNNNTGG	1 cut(s)	1527
BsuI	GTATCC	3 cut(s)	140, 1238, 2196
BsuRI	GGCC	9 cut(s)	96, 121, 376, 801, 1131, 1371, 1851, 2114, 2158
BtgI	CCRYGG	2 cut(s)	1335, 1608
BtgZI	GCGATG	3 cut(s)	1044, 1413, 1644
BtsCI	GGATG	2 cut(s)	1809, 1963
BtsIMutI	CAGTG	3 cut(s)	1362, 1481, 1500
BveI	ACCTGC	1 cut(s)	1109
Cac8I	GCNNGC	5 cut(s)	123, 199, 262, 2032, 2160
CaiI	CAGNNNCTG	4 cut(s)	122, 377, 824, 1101
CfoI	GCGC	5 cut(s)	203, 563, 659, 1534, 1623
Cfr10I	RCCGGY	1 cut(s)	1546
CseI	GACGC	2 cut(s)	39, 1937
CsiI	ACCWGGT	2 cut(s)	1138, 1864
Csp6I	GTAC	6 cut(s)	708, 1098, 1161, 1589, 1747, 1807
CspAI	ACCGGT	1 cut(s)	1546
CspCI	CAANNNNNGTGG	2 cut(s)	1921, 1956
CviQI	GTAC	6 cut(s)	708, 1098, 1161, 1589, 1747, 1807
DdeI	CTNAG	8 cut(s)	532, 1221, 1285, 1308, 2168, 2175, 2256, 2316
DinI	GGCGCC	2 cut(s)	1533, 1622
DpnI	GATC	2 cut(s)	92, 817
DpnII	GATC	2 cut(s)	90, 815
DrdI	GACNNNNNNGTC	1 cut(s)	1682
DriI	GACNNNNNGTC	1 cut(s)	1574
DseDI	GACNNNNNNGTC	1 cut(s)	1682
EaeI	YGGCCR	2 cut(s)	1129, 1849
Eam1104I	CTCTTC	1 cut(s)	835
Eam1105I	GACNNNNNGTC	1 cut(s)	1574
EarI	CTCTTC	1 cut(s)	835
EciI	GGCGGA	1 cut(s)	998
Ecl136II	GAGCTC	2 cut(s)	197, 529
Eco130I	CCWWGG	3 cut(s)	1335, 1608, 2036
Eco24I	GRGCYC	3 cut(s)	199, 272, 531
Eco31I	GGTCTC	1 cut(s)	1288
Eco47I	GGWCC	7 cut(s)	322, 1136, 1453, 1645, 1787, 1868, 2271
Eco53kI	GAGCTC	2 cut(s)	197, 529
Eco57I	CTGAAG	2 cut(s)	374, 1463
Eco88I	CYCGRG	1 cut(s)	2267
EcoICRI	GAGCTC	2 cut(s)	197, 529
EcoNI	CCTNNNNNAGG	1 cut(s)	918
EcoO109I	RGGNCCY	4 cut(s)	119, 322, 374, 1645
EcoRI	GAATTC	1 cut(s)	1817
EcoRII	CCWGG	8 cut(s)	376, 1138, 1372, 1404, 1455, 1591, 1864, 1923
EcoT14I	CCWWGG	3 cut(s)	1335, 1608, 2036
EcoT22I	ATGCAT	4 cut(s)	262, 266, 1258, 1417
EcoT38I	GRGCYC	3 cut(s)	199, 272, 531
EgeI	GGCGCC	2 cut(s)	1533, 1622
EheI	GGCGCC	2 cut(s)	1533, 1622
ErhI	CCWWGG	3 cut(s)	1335, 1608, 2036
FalI	AAGNNNNNCTT	2 cut(s)	1632, 1664
FaqI	GGGAC	3 cut(s)	335, 483, 1149
FauI	CCCGC	2 cut(s)	347, 1336
FauNDI	CATATG	1 cut(s)	1522
FblI	GTMKAC	1 cut(s)	2229
FokI	GGATG	2 cut(s)	1796, 1970
FriOI	GRGCYC	3 cut(s)	199, 272, 531
FspBI	CTAG	4 cut(s)	854, 1829, 1910, 1979
GlaI	GCGC	5 cut(s)	202, 562, 658, 1533, 1622
GsuI	CTGGAG	4 cut(s)	1395, 1441, 1614, 1677
HaeII	RGCGCY	3 cut(s)	660, 1535, 1624
HaeIII	GGCC	9 cut(s)	96, 121, 376, 801, 1131, 1371, 1851, 2114, 2158
HapII	CCGG	7 cut(s)	187, 501, 1132, 1451, 1547, 2111, 2207
HgaI	GACGC	2 cut(s)	39, 1937
HhaI	GCGC	5 cut(s)	203, 563, 659, 1534, 1623
Hin1I	GRCGYC	3 cut(s)	1532, 1621, 1929
Hin6I	GCGC	5 cut(s)	201, 561, 657, 1532, 1621
HinP1I	GCGC	5 cut(s)	201, 561, 657, 1532, 1621
HincII	GTYRAC	1 cut(s)	547
HindII	GTYRAC	1 cut(s)	547
HpaI	GTTAAC	1 cut(s)	547
HpaII	CCGG	7 cut(s)	187, 501, 1132, 1451, 1547, 2111, 2207
HphI	GGTGA	2 cut(s)	1187, 2008
Hpy166II	GTNNAC	5 cut(s)	136, 547, 1589, 1871, 2230
Hpy188I	TCNGA	8 cut(s)	90, 101, 820, 1147, 1190, 1293, 1482, 2178
Hpy188III	TCNNGA	8 cut(s)	254, 485, 1420, 1656, 1822, 1979, 1993, 2050
Hpy8I	GTNNAC	5 cut(s)	136, 547, 1589, 1871, 2230
Hpy99I	CGWCG	1 cut(s)	1973
HpyAV	CCTTC	9 cut(s)	398, 973, 1438, 1507, 1611, 1747, 1999, 2029, 2051
HpyCH4III	ACNGT	5 cut(s)	1097, 1206, 1725, 1858, 2008
HpyCH4IV	ACGT	3 cut(s)	1322, 1437, 1971
HpyF10VI	GCNNNNNNNGC	7 cut(s)	28, 102, 558, 1035, 1352, 1492, 2178
HpyF3I	CTNAG	8 cut(s)	532, 1221, 1285, 1308, 2168, 2175, 2256, 2316
HpySE526I	ACGT	3 cut(s)	1322, 1437, 1971
Hsp92I	GRCGYC	3 cut(s)	1532, 1621, 1929
HspAI	GCGC	5 cut(s)	201, 561, 657, 1532, 1621
KasI	GGCGCC	2 cut(s)	1531, 1620
KspAI	GTTAAC	1 cut(s)	547
Kzo9I	GATC	2 cut(s)	90, 815
LmnI	GCTCC	5 cut(s)	275, 740, 1376, 1459, 1769
LweI	GCATC	5 cut(s)	179, 247, 526, 859, 1621
MabI	ACCWGGT	2 cut(s)	1138, 1864
MaeI	CTAG	4 cut(s)	854, 1829, 1910, 1979
MaeII	ACGT	3 cut(s)	1322, 1437, 1971
MaeIII	GTNAC	7 cut(s)	280, 409, 931, 1729, 1972, 2014, 2151
MalI	GATC	2 cut(s)	92, 817
MboI	GATC	2 cut(s)	90, 815
MboII	GAAGA	8 cut(s)	45, 57, 60, 349, 653, 852, 1249, 2286
MfeI	CAATTG	1 cut(s)	1468
MhlI	GDGCHC	4 cut(s)	199, 272, 531, 1328
MlsI	TGGCCA	1 cut(s)	1851
MluNI	TGGCCA	1 cut(s)	1851
Mly113I	GGCGCC	2 cut(s)	1532, 1621
MlyI	GAGTC	3 cut(s)	712, 852, 888
MmeI	TCCRAC	1 cut(s)	1279
Mox20I	TGGCCA	1 cut(s)	1851
Mph1103I	ATGCAT	4 cut(s)	262, 266, 1258, 1417
MscI	TGGCCA	1 cut(s)	1851
MseI	TTAA	7 cut(s)	288, 546, 672, 786, 1947, 1962, 2145
MslI	CAYNNNNRTG	2 cut(s)	366, 2247
Msp20I	TGGCCA	1 cut(s)	1851
MspA1I	CMGCKG	1 cut(s)	1331
MspI	CCGG	7 cut(s)	187, 501, 1132, 1451, 1547, 2111, 2207
MunI	CAATTG	1 cut(s)	1468
Mva1269I	GAATGC	4 cut(s)	973, 1007, 1055, 1415
MvaI	CCWGG	8 cut(s)	378, 1140, 1374, 1406, 1457, 1593, 1866, 1925
MvnI	CGCG	3 cut(s)	201, 203, 1629
MwoI	GCNNNNNNNGC	7 cut(s)	28, 102, 558, 1035, 1352, 1492, 2178
NarI	GGCGCC	2 cut(s)	1532, 1621
NciI	CCSGG	2 cut(s)	501, 1133
NcoI	CCATGG	2 cut(s)	1335, 1608
NdeI	CATATG	1 cut(s)	1522
NdeII	GATC	2 cut(s)	90, 815
NmeAIII	GCCGAG	1 cut(s)	53
NmuCI	GTSAC	5 cut(s)	280, 409, 931, 2014, 2151
NsiI	ATGCAT	4 cut(s)	262, 266, 1258, 1417
NspI	RCATGY	2 cut(s)	264, 1256
NspV	TTCGAA	1 cut(s)	80
PaeI	GCATGC	1 cut(s)	264
PctI	GAATGC	4 cut(s)	973, 1007, 1055, 1415
PfeI	GAWTC	8 cut(s)	178, 232, 250, 358, 753, 831, 1982, 2080
PfoI	TCCNGGA	1 cut(s)	1923
PinAI	ACCGGT	1 cut(s)	1546
PleI	GAGTC	3 cut(s)	711, 851, 888
PluTI	GGCGCC	2 cut(s)	1535, 1624
PpsI	GAGTC	3 cut(s)	711, 851, 888
PpuMI	RGGWCCY	2 cut(s)	322, 1645
PsiI	TTATAA	1 cut(s)	918
Psp124BI	GAGCTC	2 cut(s)	199, 531
Psp5II	RGGWCCY	2 cut(s)	322, 1645
Psp6I	CCWGG	8 cut(s)	376, 1138, 1372, 1404, 1455, 1591, 1864, 1923
PspGI	CCWGG	8 cut(s)	376, 1138, 1372, 1404, 1455, 1591, 1864, 1923
PspPPI	RGGWCCY	2 cut(s)	322, 1645
PstI	CTGCAG	4 cut(s)	583, 748, 1357, 1837
PstNI	CAGNNNCTG	4 cut(s)	122, 377, 824, 1101
RsaI	GTAC	6 cut(s)	709, 1099, 1162, 1590, 1748, 1808
RsaNI	GTAC	6 cut(s)	708, 1098, 1161, 1589, 1747, 1807
RseI	CAYNNNNRTG	2 cut(s)	366, 2247
SacI	GAGCTC	2 cut(s)	199, 531
SaqAI	TTAA	7 cut(s)	288, 546, 672, 786, 1947, 1962, 2145
Sau3AI	GATC	2 cut(s)	90, 815
ScaI	AGTACT	1 cut(s)	1162
SchI	GAGTC	3 cut(s)	712, 852, 888
SduI	GDGCHC	4 cut(s)	199, 272, 531, 1328
SexAI	ACCWGGT	2 cut(s)	1138, 1864
SfaNI	GCATC	5 cut(s)	179, 247, 526, 859, 1621
SfcI	CTRYAG	7 cut(s)	579, 660, 744, 1353, 1833, 2084, 2230
SfoI	GGCGCC	2 cut(s)	1533, 1622
SfuI	TTCGAA	1 cut(s)	80
SinI	GGWCC	7 cut(s)	322, 1136, 1453, 1645, 1787, 1868, 2271
SmiMI	CAYNNNNRTG	2 cut(s)	366, 2247
SmlI	CTYRAG	2 cut(s)	296, 1277
SmoI	CTYRAG	2 cut(s)	296, 1277
SphI	GCATGC	1 cut(s)	264
SsiI	CCGC	7 cut(s)	354, 550, 726, 900, 983, 1329, 1627
SspDI	GGCGCC	2 cut(s)	1531, 1620
SspMI	CTAG	4 cut(s)	854, 1829, 1910, 1979
SstI	GAGCTC	2 cut(s)	199, 531
StyI	CCWWGG	3 cut(s)	1335, 1608, 2036
TaaI	ACNGT	5 cut(s)	1097, 1206, 1725, 1858, 2008
TaiI	ACGT	3 cut(s)	1325, 1440, 1974
TaqI	TCGA	8 cut(s)	42, 80, 193, 795, 1383, 1740, 1860, 1890
TatI	WGTACW	2 cut(s)	1160, 1746
TauI	GCSGC	1 cut(s)	902
TfiI	GAWTC	8 cut(s)	178, 232, 250, 358, 753, 831, 1982, 2080
Tru1I	TTAA	7 cut(s)	288, 546, 672, 786, 1947, 1962, 2145
Tru9I	TTAA	7 cut(s)	288, 546, 672, 786, 1947, 1962, 2145
TscAI	CASTG	3 cut(s)	1369, 1481, 1507
TseFI	GTSAC	5 cut(s)	280, 409, 931, 2014, 2151
Tsp45I	GTSAC	5 cut(s)	280, 409, 931, 2014, 2151
TspDTI	ATGAA	4 cut(s)	745, 1131, 1164, 1526
TspGWI	ACGGA	3 cut(s)	485, 1068, 1800
TspRI	CASTG	3 cut(s)	1369, 1481, 1507
VpaK11BI	GGWCC	7 cut(s)	322, 1136, 1453, 1645, 1787, 1868, 2271
XagI	CCTNNNNNAGG	1 cut(s)	918
XapI	RAATTY	8 cut(s)	635, 995, 1151, 1554, 1598, 1670, 1817, 2260
XbaI	TCTAGA	1 cut(s)	1978
XceI	RCATGY	2 cut(s)	264, 1256
XmiI	GTMKAC	1 cut(s)	2229
XspI	CTAG	4 cut(s)	854, 1829, 1910, 1979
ZrmI	AGTACT	1 cut(s)	1162
Zsp2I	ATGCAT	4 cut(s)	262, 266, 1258, 1417

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.