pycom12g21630

Splicing factor 3B subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0021189
Physical Location & Seq
Forward (+)
22776931 .. 22778293
1363 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 984 bp
ATGAAAATCAGGTGGTCACTGCTCTCTCTATCTCTCTCTCTCTTGCTGCTCTTGCTGAGGCTGCTGCCCCATACGGGTATTAGATCTCACCGCGGTAAGGTTTTGGCTGCCTTCTTGAAGGCAGCTGGTTTCGTTATTCCCCTCGTGGATGCAATGTATTTCAAAAACTTCTGGATTAGAAGGATGGCTTTGGAGAGGAAAAACTACAGGCAATTGGTGGGAAACAACTGCGGAGATAGCAAACACAGTGGATGCTCTCGGTTGGAGGAGCTTCTCATTGACAGTGTCCTATATGCTTTCCAAGAGCAGACCAGTGACGATGTTAATGCGATGCTTAATGGATTTGGTGCAGTTGTGAATTCTCTTGGGCACCTTCCTCCGATTTGTCGTAACACTAAGTGGCGCTTGAATAACAAGGGTGCAAAGGCAGACACAAGCTACCGATCTTATTTCGAGAACTGCTGTTGTCATGAAGCAGTGCCAGGTGGAGCAAATGATGGGTCATCTTGGTGTCTGTCTTTTACGGTATTTCCAGCACTGATGAACGAGTATCGTGTTCCAGAGCTTAATGTGCAGAATGGTGTGTTGAAATCCCTCTCTTTCTTGTTCGATTACATTGGTGAGATGGGGAAAGACTACATCTACGCAGTGACCCCGTTGCTCGAGGATGCCCTCATGGATAGAGAGATCGTTCACAGGCAAACTGCAGCTTCTGCTGTCAAGCACATGGCTCTAGGAGTAGCAGGATTGGGATGCGAGGAGGACGCATTAGTCCACTTGCTGAACTACGTCCGGCCAAACATATTCGAGACATCCCCACACGTTATCAATGCTGTCATGGAAGCGATTGAAGGGATAGGTGTATGGGAAGATTTACAATCGCTGTATATCGGAGCTCAAGATGCTGTCGTCGCGTCGTACCCATTGCTCGAGGACGAGGAGCATAATGTATATACTAGGGCCGAGCTAATGACGTTTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
SF3b1

Protein Analysis

328

Amino Acids

36.57

Weight (kDa)

5.81

Isoelectric Point (pI)

35.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021189)

Species Orthologous Gene IDs
malus_domestica MD12G1233900.v1.1
pyrus_communis pycom12g21630
rosa_chinensis RchiOBHm_Chr5g0043931
rosa_samantha Rh5BG301300 Rh5DG310500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 770
AccB1I GGYRCC 1 cut(s) 369
AccII CGCG 2 cut(s) 93, 914
AciI CCGC 3 cut(s) 91, 93, 231
AcoI YGGCCR 1 cut(s) 794
AcsI RAATTY 1 cut(s) 358
AdeI CACNNNGTG 1 cut(s) 399
AfaI GTAC 1 cut(s) 920
AfiI CCNNNNNNNGG 2 cut(s) 74, 97
AflIII ACRYGT 1 cut(s) 820
AgsI TTSAA 5 cut(s) 118, 163, 409, 589, 851
AjnI CCWGG 1 cut(s) 481
AluBI AGCT 7 cut(s) 125, 271, 438, 565, 710, 896, 967
AluI AGCT 7 cut(s) 125, 271, 438, 565, 710, 896, 967
Alw21I GWGCWC 1 cut(s) 898
Alw26I GTCTC 1 cut(s) 803
AlwNI CAGNNNCTG 1 cut(s) 713
Ama87I CYCGRG 2 cut(s) 662, 929
AoxI GGCC 2 cut(s) 794, 960
ApeKI GCWGC 6 cut(s) 46, 61, 64, 107, 122, 707
ApoI RAATTY 1 cut(s) 358
ArsI GACNNNNNNTTYG 2 cut(s) 485, 517
AspLEI GCGC 1 cut(s) 405
AspS9I GGNCC 1 cut(s) 960
AsuHPI GGTGA 2 cut(s) 80, 632
AvaI CYCGRG 2 cut(s) 662, 929
BaeGI GKGCMC 1 cut(s) 372
BanI GGYRCC 1 cut(s) 369
BanII GRGCYC 1 cut(s) 898
BauI CACGAG 1 cut(s) 143
Bbv12I GWGCWC 1 cut(s) 898
BbvCI CCTCAGC 1 cut(s) 56
BbvI GCAGC 6 cut(s) 33, 48, 51, 94, 134, 719
BccI CCATC 3 cut(s) 178, 491, 619
BcgI CGANNNNNNTGC 2 cut(s) 308, 342
BciT130I CCWGG 1 cut(s) 483
BcoDI GTCTC 1 cut(s) 803
BfaI CTAG 2 cut(s) 734, 957
BfmI CTRYAG 2 cut(s) 205, 705
BfoI RGCGCY 1 cut(s) 406
BglII AGATCT 1 cut(s) 83
BisI GCNGC 6 cut(s) 47, 62, 65, 108, 123, 708
BlsI GCNGC 6 cut(s) 48, 63, 66, 109, 124, 709
Bme1390I CCNGG 1 cut(s) 483
BmeT110I CYCGRG 2 cut(s) 662, 929
BmgT120I GGNCC 1 cut(s) 960
BmiI GGNNCC 1 cut(s) 371
BmrFI CCNGG 1 cut(s) 483
BmsI GCATC 6 cut(s) 139, 242, 321, 658, 743, 892
Bpu10I CCTNAGC 1 cut(s) 56
BpuEI CTTGAG 1 cut(s) 882
BsaJI CCNNGG 1 cut(s) 91
Bsc4I CCNNNNNNNGG 2 cut(s) 74, 97
Bse1I ACTGG 1 cut(s) 312
Bse3DI GCAATG 2 cut(s) 159, 923
BseBI CCWGG 1 cut(s) 483
BseDI CCNNGG 1 cut(s) 91
BseGI GGATG 6 cut(s) 154, 189, 257, 673, 758, 812
BseLI CCNNNNNNNGG 2 cut(s) 74, 97
BseMI GCAATG 2 cut(s) 159, 923
BseMII CTCAG 1 cut(s) 47
BseNI ACTGG 1 cut(s) 312
BseRI GAGGAG 3 cut(s) 281, 773, 953
BseSI GKGCMC 1 cut(s) 372
BseXI GCAGC 6 cut(s) 33, 48, 51, 94, 134, 719
BsgI GTGCAG 2 cut(s) 369, 593
Bsh1236I CGCG 2 cut(s) 93, 914
BshFI GGCC 2 cut(s) 796, 962
BshNI GGYRCC 1 cut(s) 369
BsiHKAI GWGCWC 1 cut(s) 898
BsiHKCI CYCGRG 2 cut(s) 662, 929
BsiSI CCGG 1 cut(s) 793
BslI CCNNNNNNNGG 2 cut(s) 74, 97
BsmAI GTCTC 1 cut(s) 803
BsnI GGCC 2 cut(s) 796, 962
BsoBI CYCGRG 2 cut(s) 662, 929
Bsp1286I GDGCHC 2 cut(s) 372, 898
Bsp143I GATC 3 cut(s) 83, 443, 687
BspACI CCGC 3 cut(s) 91, 93, 231
BspANI GGCC 2 cut(s) 796, 962
BspCNI CTCAG 1 cut(s) 48
BspFNI CGCG 2 cut(s) 93, 914
BspHI TCATGA 1 cut(s) 469
BspLI GGNNCC 1 cut(s) 371
BspMAI CTGCAG 1 cut(s) 709
BspT107I GGYRCC 1 cut(s) 369
BsrDI GCAATG 2 cut(s) 159, 923
BsrI ACTGG 1 cut(s) 312
BssECI CCNNGG 1 cut(s) 91
BssMI GATC 3 cut(s) 83, 443, 687
BssSI CACGAG 1 cut(s) 143
Bst2BI CACGAG 1 cut(s) 143
Bst2UI CCWGG 1 cut(s) 483
Bst4CI ACNGT 3 cut(s) 248, 284, 526
BstAPI GCANNNNNTGC 1 cut(s) 713
BstDEI CTNAG 2 cut(s) 56, 396
BstDSI CCRYGG 1 cut(s) 91
BstF5I GGATG 6 cut(s) 154, 189, 257, 673, 758, 812
BstFNI CGCG 2 cut(s) 93, 914
BstH2I RGCGCY 1 cut(s) 406
BstHHI GCGC 1 cut(s) 405
BstKTI GATC 3 cut(s) 86, 446, 690
BstMAI GTCTC 1 cut(s) 803
BstMBI GATC 3 cut(s) 83, 443, 687
BstMWI GCNNNNNNNGC 7 cut(s) 52, 61, 237, 571, 713, 902, 911
BstNI CCWGG 1 cut(s) 483
BstSCI CCNGG 1 cut(s) 481
BstSFI CTRYAG 2 cut(s) 205, 705
BstSLI GKGCMC 1 cut(s) 372
BstUI CGCG 2 cut(s) 93, 914
BstV1I GCAGC 6 cut(s) 33, 48, 51, 94, 134, 719
BstX2I RGATCY 1 cut(s) 83
BstYI RGATCY 1 cut(s) 83
BsuRI GGCC 2 cut(s) 796, 962
BtgI CCRYGG 1 cut(s) 91
BtgZI GCGATG 1 cut(s) 344
BtsCI GGATG 6 cut(s) 154, 189, 257, 673, 758, 812
BtsI GCAGTG 3 cut(s) 17, 483, 654
BtsIMutI CAGTG 7 cut(s) 17, 253, 289, 319, 483, 536, 654
CaiI CAGNNNCTG 1 cut(s) 713
CciI TCATGA 1 cut(s) 469
CfoI GCGC 1 cut(s) 405
Cfr13I GGNCC 1 cut(s) 960
Cfr42I CCGCGG 1 cut(s) 94
CseI GACGC 2 cut(s) 773, 903
Csp6I GTAC 1 cut(s) 919
CspCI CAANNNNNGTGG 2 cut(s) 229, 264
CviAII CATG 4 cut(s) 470, 676, 727, 838
CviQI GTAC 1 cut(s) 919
DdeI CTNAG 2 cut(s) 56, 396
DpnI GATC 3 cut(s) 85, 445, 689
DpnII GATC 3 cut(s) 83, 443, 687
DraIII CACNNNGTG 1 cut(s) 399
DrdI GACNNNNNNGTC 1 cut(s) 770
DseDI GACNNNNNNGTC 1 cut(s) 770
EaeI YGGCCR 1 cut(s) 794
Ecl136II GAGCTC 1 cut(s) 896
Eco24I GRGCYC 1 cut(s) 898
Eco53kI GAGCTC 1 cut(s) 896
Eco88I CYCGRG 2 cut(s) 662, 929
EcoICRI GAGCTC 1 cut(s) 896
EcoRI GAATTC 1 cut(s) 358
EcoRII CCWGG 1 cut(s) 481
EcoT38I GRGCYC 1 cut(s) 898
FaeI CATG 4 cut(s) 473, 679, 730, 841
FalI AAGNNNNNCTT 4 cut(s) 172, 204, 389, 421
FatI CATG 4 cut(s) 469, 675, 726, 837
Fnu4HI GCNGC 6 cut(s) 47, 62, 65, 108, 123, 708
FokI GGATG 6 cut(s) 161, 196, 264, 680, 765, 799
FriOI GRGCYC 1 cut(s) 898
Fsp4HI GCNGC 6 cut(s) 47, 62, 65, 108, 123, 708
FspBI CTAG 2 cut(s) 734, 957
GlaI GCGC 1 cut(s) 404
GluI GCNGC 6 cut(s) 47, 62, 65, 108, 123, 708
HaeII RGCGCY 1 cut(s) 406
HaeIII GGCC 2 cut(s) 796, 962
HapII CCGG 1 cut(s) 793
HgaI GACGC 2 cut(s) 773, 903
HhaI GCGC 1 cut(s) 405
Hin1II CATG 4 cut(s) 473, 679, 730, 841
Hin6I GCGC 1 cut(s) 403
HinP1I GCGC 1 cut(s) 403
HpaII CCGG 1 cut(s) 793
HphI GGTGA 2 cut(s) 80, 632
Hpy166II GTNNAC 2 cut(s) 694, 775
Hpy188I TCNGA 2 cut(s) 381, 893
Hpy188III TCNNGA 7 cut(s) 115, 172, 454, 470, 560, 808, 899
Hpy8I GTNNAC 2 cut(s) 694, 775
Hpy99I CGWCG 2 cut(s) 914, 919
HpyAV CCTTC 5 cut(s) 112, 121, 174, 383, 845
HpyCH4III ACNGT 3 cut(s) 248, 284, 526
HpyCH4IV ACGT 3 cut(s) 789, 822, 974
HpyCH4V TGCA 5 cut(s) 152, 350, 422, 574, 707
HpyF10VI GCNNNNNNNGC 7 cut(s) 52, 61, 237, 571, 713, 902, 911
HpyF3I CTNAG 2 cut(s) 56, 396
HpySE526I ACGT 3 cut(s) 789, 822, 974
Hsp92II CATG 4 cut(s) 473, 679, 730, 841
HspAI GCGC 1 cut(s) 403
KspI CCGCGG 1 cut(s) 94
Kzo9I GATC 3 cut(s) 83, 443, 687
LmnI GCTCC 4 cut(s) 268, 488, 893, 940
Lsp1109I GCAGC 6 cut(s) 33, 48, 51, 94, 134, 719
LweI GCATC 6 cut(s) 139, 242, 321, 658, 743, 892
MaeI CTAG 2 cut(s) 734, 957
MaeII ACGT 3 cut(s) 789, 822, 974
MaeIII GTNAC 4 cut(s) 15, 314, 389, 649
MalI GATC 3 cut(s) 85, 445, 689
MboI GATC 3 cut(s) 83, 443, 687
MboII GAAGA 1 cut(s) 881
MfeI CAATTG 1 cut(s) 212
MflI RGATCY 1 cut(s) 83
MhlI GDGCHC 2 cut(s) 372, 898
MluCI AATT 2 cut(s) 212, 358
MmeI TCCRAC 1 cut(s) 243
MseI TTAA 3 cut(s) 324, 336, 567
MslI CAYNNNNRTG 1 cut(s) 508
MspA1I CMGCKG 2 cut(s) 93, 125
MspI CCGG 1 cut(s) 793
MspR9I CCNGG 1 cut(s) 483
MunI CAATTG 1 cut(s) 212
MvaI CCWGG 1 cut(s) 483
MvnI CGCG 2 cut(s) 93, 914
MwoI GCNNNNNNNGC 7 cut(s) 52, 61, 237, 571, 713, 902, 911
NdeII GATC 3 cut(s) 83, 443, 687
NlaIII CATG 4 cut(s) 473, 679, 730, 841
NlaIV GGNNCC 1 cut(s) 371
NmuCI GTSAC 3 cut(s) 15, 314, 649
PaeR7I CTCGAG 2 cut(s) 662, 929
PagI TCATGA 1 cut(s) 469
PflFI GACNNNGTC 1 cut(s) 284
PkrI GCNGC 6 cut(s) 48, 63, 66, 109, 124, 709
Psp124BI GAGCTC 1 cut(s) 898
Psp6I CCWGG 1 cut(s) 481
PspGI CCWGG 1 cut(s) 481
PspN4I GGNNCC 1 cut(s) 371
PspPI GGNCC 1 cut(s) 960
PspXI VCTCGAGB 2 cut(s) 662, 929
PstI CTGCAG 1 cut(s) 709
PstNI CAGNNNCTG 1 cut(s) 713
PsuI RGATCY 1 cut(s) 83
PsyI GACNNNGTC 1 cut(s) 284
PvuII CAGCTG 1 cut(s) 125
RsaI GTAC 1 cut(s) 920
RsaNI GTAC 1 cut(s) 919
RseI CAYNNNNRTG 1 cut(s) 508
SacI GAGCTC 1 cut(s) 898
SacII CCGCGG 1 cut(s) 94
SaqAI TTAA 3 cut(s) 324, 336, 567
SatI GCNGC 6 cut(s) 47, 62, 65, 108, 123, 708
Sau3AI GATC 3 cut(s) 83, 443, 687
Sau96I GGNCC 1 cut(s) 960
ScrFI CCNGG 1 cut(s) 483
SduI GDGCHC 2 cut(s) 372, 898
SfaNI GCATC 6 cut(s) 139, 242, 321, 658, 743, 892
SfcI CTRYAG 2 cut(s) 205, 705
Sfr274I CTCGAG 2 cut(s) 662, 929
Sfr303I CCGCGG 1 cut(s) 94
SgrBI CCGCGG 1 cut(s) 94
SlaI CTCGAG 2 cut(s) 662, 929
SmiMI CAYNNNNRTG 1 cut(s) 508
SmlI CTYRAG 3 cut(s) 662, 897, 929
SmoI CTYRAG 3 cut(s) 662, 897, 929
Sse9I AATT 2 cut(s) 212, 358
SsiI CCGC 3 cut(s) 91, 93, 231
SspMI CTAG 2 cut(s) 734, 957
SstI GAGCTC 1 cut(s) 898
StyD4I CCNGG 1 cut(s) 481
TaaI ACNGT 3 cut(s) 248, 284, 526
TaiI ACGT 3 cut(s) 792, 825, 977
TaqI TCGA 5 cut(s) 453, 609, 663, 807, 930
TasI AATT 2 cut(s) 212, 358
Tru1I TTAA 3 cut(s) 324, 336, 567
Tru9I TTAA 3 cut(s) 324, 336, 567
TscAI CASTG 7 cut(s) 24, 253, 289, 319, 483, 543, 654
TseFI GTSAC 3 cut(s) 15, 314, 649
TseI GCWGC 6 cut(s) 46, 61, 64, 107, 122, 707
Tsp45I GTSAC 3 cut(s) 15, 314, 649
TspDTI ATGAA 3 cut(s) 17, 486, 557
TspRI CASTG 7 cut(s) 24, 253, 289, 319, 483, 543, 654
Tth111I GACNNNGTC 1 cut(s) 284
XapI RAATTY 1 cut(s) 358
XhoI CTCGAG 2 cut(s) 662, 929
XspI CTAG 2 cut(s) 734, 957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.