Rh5BG301300

Splicing factor 3B subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0021189
Physical Location & Seq
Reverse (-)
38886553 .. 38886885
333 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG301300.1

Sequence Viewer

Length: 333 bp
ATGGCTTTAGGAGTAGCTGGATTGGGTTGTGAGGAGGCGTTAGTCCACTTGCTGAACTATGTCTGGCCAAACATATTTGAGACATCCCCACATGTTATAAATGCTGTTATGGAAGCCATTGAAGGGATGAGGGTGGCTTTGGGTCCTGCTGTTGTTCTCAATTACTGTCTCCAGGGGCTGTTCCATCCTGCTAGGAAGGTTAGGGAGGCGTACTGGAAGATTTACAACTCACTCTATATTGGAGCTCAAGATGCTCTTGTGGCGGCCTACCCTTTGCTGGAGGACGAGGAGCATAATGTGTATACTAGGCCAGAGCTGACAATGTTCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
SF3b1

Protein Analysis

110

Amino Acids

12.33

Weight (kDa)

5.01

Isoelectric Point (pI)

40.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021189)

Species Orthologous Gene IDs
malus_domestica MD12G1233900.v1.1
pyrus_communis pycom12g21630
rosa_chinensis RchiOBHm_Chr5g0043931
rosa_samantha Rh5BG301300 Rh5DG310500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 98
AccI GTMKAC 1 cut(s) 302
AciI CCGC 1 cut(s) 263
AcoI YGGCCR 1 cut(s) 65
AfaI GTAC 1 cut(s) 212
AfiI CCNNNNNNNGG 2 cut(s) 123, 277
AflIII ACRYGT 1 cut(s) 91
AgsI TTSAA 1 cut(s) 122
AjnI CCWGG 1 cut(s) 171
AluBI AGCT 3 cut(s) 17, 245, 316
AluI AGCT 3 cut(s) 17, 245, 316
Alw21I GWGCWC 1 cut(s) 247
Alw26I GTCTC 2 cut(s) 74, 173
AlwNI CAGNNNCTG 1 cut(s) 178
AoxI GGCC 3 cut(s) 65, 264, 308
AspS9I GGNCC 1 cut(s) 143
AvaII GGWCC 1 cut(s) 143
BalI TGGCCA 1 cut(s) 67
BanII GRGCYC 1 cut(s) 247
Bbv12I GWGCWC 1 cut(s) 247
BccI CCATC 1 cut(s) 192
BciT130I CCWGG 1 cut(s) 173
BcoDI GTCTC 2 cut(s) 74, 173
BfaI CTAG 2 cut(s) 192, 306
BisI GCNGC 1 cut(s) 264
BlsI GCNGC 1 cut(s) 265
Bme1390I CCNGG 1 cut(s) 173
Bme18I GGWCC 1 cut(s) 143
BmgT120I GGNCC 1 cut(s) 143
BmiI GGNNCC 1 cut(s) 144
BmrFI CCNGG 1 cut(s) 173
BmsI GCATC 1 cut(s) 241
BpmI CTGGAG 2 cut(s) 155, 299
BpuEI CTTGAG 1 cut(s) 231
BsaJI CCNNGG 1 cut(s) 172
Bsc4I CCNNNNNNNGG 2 cut(s) 123, 277
Bse1I ACTGG 1 cut(s) 218
BseBI CCWGG 1 cut(s) 173
BseDI CCNNGG 1 cut(s) 172
BseGI GGATG 3 cut(s) 83, 132, 184
BseLI CCNNNNNNNGG 2 cut(s) 123, 277
BseNI ACTGG 1 cut(s) 218
BseRI GAGGAG 2 cut(s) 47, 302
BshFI GGCC 3 cut(s) 67, 266, 310
BsiHKAI GWGCWC 1 cut(s) 247
BslI CCNNNNNNNGG 2 cut(s) 123, 277
BsmAI GTCTC 2 cut(s) 74, 173
BsnI GGCC 3 cut(s) 67, 266, 310
Bsp1286I GDGCHC 1 cut(s) 247
BspACI CCGC 1 cut(s) 263
BspANI GGCC 3 cut(s) 67, 266, 310
BspLI GGNNCC 1 cut(s) 144
BsrI ACTGG 1 cut(s) 218
BssECI CCNNGG 1 cut(s) 172
BssNAI GTATAC 1 cut(s) 303
Bst1107I GTATAC 1 cut(s) 303
Bst2UI CCWGG 1 cut(s) 173
Bst4CI ACNGT 1 cut(s) 167
BstF5I GGATG 3 cut(s) 83, 132, 184
BstMAI GTCTC 2 cut(s) 74, 173
BstMWI GCNNNNNNNGC 2 cut(s) 251, 260
BstNI CCWGG 1 cut(s) 173
BstNSI RCATGY 1 cut(s) 95
BstSCI CCNGG 1 cut(s) 171
BstZ17I GTATAC 1 cut(s) 303
BsuRI GGCC 3 cut(s) 67, 266, 310
BtsCI GGATG 3 cut(s) 83, 132, 184
CaiI CAGNNNCTG 1 cut(s) 178
Cfr13I GGNCC 1 cut(s) 143
Csp6I GTAC 1 cut(s) 211
CviAII CATG 1 cut(s) 92
CviQI GTAC 1 cut(s) 211
EaeI YGGCCR 1 cut(s) 65
Ecl136II GAGCTC 1 cut(s) 245
Eco24I GRGCYC 1 cut(s) 247
Eco47I GGWCC 1 cut(s) 143
Eco53kI GAGCTC 1 cut(s) 245
EcoICRI GAGCTC 1 cut(s) 245
EcoO109I RGGNCCY 1 cut(s) 143
EcoRII CCWGG 1 cut(s) 171
EcoT38I GRGCYC 1 cut(s) 247
FaeI CATG 1 cut(s) 95
FalI AAGNNNNNCTT 2 cut(s) 240, 272
FatI CATG 1 cut(s) 91
FauNDI CATATG 1 cut(s) 329
FblI GTMKAC 1 cut(s) 302
Fnu4HI GCNGC 1 cut(s) 264
FokI GGATG 3 cut(s) 70, 139, 171
FriOI GRGCYC 1 cut(s) 247
Fsp4HI GCNGC 1 cut(s) 264
FspBI CTAG 2 cut(s) 192, 306
GluI GCNGC 1 cut(s) 264
GsuI CTGGAG 2 cut(s) 155, 299
HaeIII GGCC 3 cut(s) 67, 266, 310
Hin1II CATG 1 cut(s) 95
Hpy166II GTNNAC 2 cut(s) 46, 303
Hpy188III TCNNGA 1 cut(s) 248
Hpy8I GTNNAC 2 cut(s) 46, 303
HpyAV CCTTC 2 cut(s) 116, 190
HpyCH4III ACNGT 1 cut(s) 167
HpyF10VI GCNNNNNNNGC 2 cut(s) 251, 260
Hsp92II CATG 1 cut(s) 95
LmnI GCTCC 2 cut(s) 242, 289
LpnPI CCDG 9 cut(s) 3, 49, 158, 159, 185, 199, 201, 263, 324
LweI GCATC 1 cut(s) 241
MaeI CTAG 2 cut(s) 192, 306
MboII GAAGA 1 cut(s) 229
MhlI GDGCHC 1 cut(s) 247
MlsI TGGCCA 1 cut(s) 67
MluCI AATT 1 cut(s) 160
MluNI TGGCCA 1 cut(s) 67
MnlI CCTC 6 cut(s) 25, 28, 123, 199, 274, 280
Mox20I TGGCCA 1 cut(s) 67
MscI TGGCCA 1 cut(s) 67
Msp20I TGGCCA 1 cut(s) 67
MspR9I CCNGG 1 cut(s) 173
MvaI CCWGG 1 cut(s) 173
MwoI GCNNNNNNNGC 2 cut(s) 251, 260
NdeI CATATG 1 cut(s) 329
NlaIII CATG 1 cut(s) 95
NlaIV GGNNCC 1 cut(s) 144
NspI RCATGY 1 cut(s) 95
PciI ACATGT 1 cut(s) 91
PkrI GCNGC 1 cut(s) 265
PpuMI RGGWCCY 1 cut(s) 143
PscI ACATGT 1 cut(s) 91
PsiI TTATAA 1 cut(s) 98
Psp124BI GAGCTC 1 cut(s) 247
Psp5II RGGWCCY 1 cut(s) 143
Psp6I CCWGG 1 cut(s) 171
PspGI CCWGG 1 cut(s) 171
PspN4I GGNNCC 1 cut(s) 144
PspPI GGNCC 1 cut(s) 143
PspPPI RGGWCCY 1 cut(s) 143
PstNI CAGNNNCTG 1 cut(s) 178
RsaI GTAC 1 cut(s) 212
RsaNI GTAC 1 cut(s) 211
SacI GAGCTC 1 cut(s) 247
SatI GCNGC 1 cut(s) 264
Sau96I GGNCC 1 cut(s) 143
ScrFI CCNGG 1 cut(s) 173
SduI GDGCHC 1 cut(s) 247
SetI ASST 4 cut(s) 19, 201, 247, 318
SfaNI GCATC 1 cut(s) 241
SinI GGWCC 1 cut(s) 143
SmlI CTYRAG 1 cut(s) 246
SmoI CTYRAG 1 cut(s) 246
Sse9I AATT 1 cut(s) 160
SsiI CCGC 1 cut(s) 263
SspMI CTAG 2 cut(s) 192, 306
SstI GAGCTC 1 cut(s) 247
StyD4I CCNGG 1 cut(s) 171
TaaI ACNGT 1 cut(s) 167
TasI AATT 1 cut(s) 160
TauI GCSGC 1 cut(s) 266
TspDTI ATGAA 1 cut(s) 316
VpaK11BI GGWCC 1 cut(s) 143
XceI RCATGY 1 cut(s) 95
XmiI GTMKAC 1 cut(s) 302
XspI CTAG 2 cut(s) 192, 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.