pycom12g22850

RNA polymerase-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0024684
Physical Location & Seq
Forward (+)
23622451 .. 23632238
9788 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 678 bp
ATGGAGGAAGTGACTCTAGAGGTGATGATTCTGACGATGGTCGTGGCTATGCAAGCAGGAAGCCCTCTGGACCTCAATGATCTTTGCAAGGATGAAGACGATAGGCAGAAGCTCGCAGAGATGTCTGAACTTCAAAGAGAGTTAATTTTGTCCGAAAGAGCACAGAAGAAAGATGACAAGAGTTTAAAGGAGAATTTCCGACCAAAGTGGGACAAGGGGGAGGCCCCACAGTCCCGGAAAGAGGCTCCCCCTCTTACAACCTCTCAGGTGCGTTCATCAGCCAGATCTGCTGACAGGGCAACTTTCAAGGATGATGCATTGAATGAGTTAATAGCAAAATGTTTGAAGCGGCAGGACCATGAGGCTCACCTCAAGTTGAGAGATGTTTCTCGTAGTGAAGATGATGGATCATCAGGGGATGATGCAATGATTGACAGTGATGATGAAAGGTCAGAGGGACTAACTTTTGATGATATAAAAGAGATTACCATTCGAAGGTCGAAACTTGCAAAGTGGTTTATGGAGCCCTTCCTGGAAGAGTTAATTGTGGTTTGCGTTGTGAGGGTTGGAATTAGGAGGTCAAAATCTAGGACTATCAGTGGTGGGAGCAGTACGGGCAAACATGGCGGCAACTGCAGCAGCTTTGGAAGCTGCGGCTGGTGCGGGGAAATTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000122 GO:0000428 GO:0000988 GO:0000989 GO:0000993 GO:0001076 GO:0001098 GO:0001099 GO:0001701 GO:0001704 GO:0001706 GO:0001711 GO:0001824 GO:0001832 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0007275 GO:0007369 GO:0007492 GO:0008023 GO:0008150 GO:0008152 GO:0008213 GO:0009653 GO:0009790 GO:0009792 GO:0009888 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009909 GO:0009910 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010638 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016591 GO:0016593 GO:0018022 GO:0018023 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0019827 GO:0019899 GO:0030154 GO:0030880 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032259 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0033043 GO:0033044 GO:0034243 GO:0034968 GO:0035987 GO:0036211 GO:0040007 GO:0043009 GO:0043170 GO:0043175 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045165 GO:0045309 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048581 GO:0048589 GO:0048598 GO:0048646 GO:0048831 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050815 GO:0051093 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051219 GO:0051239 GO:0051241 GO:0051246 GO:0051247 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0051568 GO:0051569 GO:0051571 GO:0055029 GO:0060255 GO:0060795 GO:0061695 GO:0065007 GO:0070013 GO:0070063 GO:0071704 GO:0071840 GO:0080090 GO:0080182 GO:0097159 GO:0098727 GO:0099122 GO:0140110 GO:1901363 GO:1901564 GO:1902275 GO:1902494 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1905269 GO:1990234 GO:1990269 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Plus-3

Protein Analysis

226

Amino Acids

25.21

Weight (kDa)

5.15

Isoelectric Point (pI)

58.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Plus-3 PF03126 158 - 195 4e-07 Plus-3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0024684)

Species Orthologous Gene IDs
pyrus_communis pycom12g22700 pycom12g22760 pycom12g22850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 349, 627, 654, 663
AclWI GGATC 1 cut(s) 415
AcsI RAATTY 1 cut(s) 193
AfaI GTAC 1 cut(s) 613
AfiI CCNNNNNNNGG 2 cut(s) 241, 495
AgsI TTSAA 4 cut(s) 134, 307, 322, 346
AjnI CCWGG 1 cut(s) 531
AluBI AGCT 3 cut(s) 112, 642, 651
AluI AGCT 3 cut(s) 112, 642, 651
Alw21I GWGCWC 1 cut(s) 163
AlwI GGATC 1 cut(s) 415
AoxI GGCC 1 cut(s) 222
ApeKI GCWGC 3 cut(s) 636, 639, 651
ApoI RAATTY 1 cut(s) 193
AspS9I GGNCC 3 cut(s) 70, 223, 355
AsuC2I CCSGG 1 cut(s) 235
AsuHPI GGTGA 2 cut(s) 34, 359
AsuII TTCGAA 1 cut(s) 493
AvaII GGWCC 2 cut(s) 70, 355
BanII GRGCYC 1 cut(s) 528
BbsI GAAGAC 1 cut(s) 102
Bbv12I GWGCWC 1 cut(s) 163
BbvI GCAGC 3 cut(s) 638, 648, 651
BccI CCATC 2 cut(s) 31, 398
BciT130I CCWGG 1 cut(s) 533
BcnI CCSGG 1 cut(s) 235
BfaI CTAG 2 cut(s) 17, 588
BfmI CTRYAG 1 cut(s) 634
BglII AGATCT 1 cut(s) 284
BisI GCNGC 6 cut(s) 350, 628, 637, 640, 652, 655
BlsI GCNGC 6 cut(s) 351, 629, 638, 641, 653, 656
Bme1390I CCNGG 2 cut(s) 235, 533
Bme18I GGWCC 2 cut(s) 70, 355
BmgT120I GGNCC 3 cut(s) 70, 223, 355
BmiI GGNNCC 3 cut(s) 225, 246, 525
BmrFI CCNGG 2 cut(s) 235, 533
BmsI GCATC 2 cut(s) 304, 412
BoxI GACNNNNGTC 1 cut(s) 38
BpiI GAAGAC 1 cut(s) 102
Bpu14I TTCGAA 1 cut(s) 493
BpuEI CTTGAG 1 cut(s) 356
BpuMI CCSGG 1 cut(s) 235
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 495
Bse3DI GCAATG 1 cut(s) 432
BseBI CCWGG 1 cut(s) 533
BseGI GGATG 3 cut(s) 97, 316, 424
BseLI CCNNNNNNNGG 2 cut(s) 241, 495
BseMI GCAATG 1 cut(s) 432
BseMII CTCAG 1 cut(s) 278
BseXI GCAGC 3 cut(s) 638, 648, 651
BshFI GGCC 1 cut(s) 224
BsiHKAI GWGCWC 1 cut(s) 163
BsiSI CCGG 1 cut(s) 235
BslFI GGGAC 3 cut(s) 217, 224, 471
BslI CCNNNNNNNGG 2 cut(s) 241, 495
BsmFI GGGAC 3 cut(s) 217, 224, 471
BsnI GGCC 1 cut(s) 224
Bsp119I TTCGAA 1 cut(s) 493
Bsp1286I GDGCHC 2 cut(s) 163, 528
Bsp143I GATC 3 cut(s) 79, 284, 407
BspACI CCGC 4 cut(s) 349, 627, 654, 663
BspANI GGCC 1 cut(s) 224
BspCNI CTCAG 1 cut(s) 277
BspLI GGNNCC 3 cut(s) 225, 246, 525
BspMAI CTGCAG 1 cut(s) 638
BspPI GGATC 1 cut(s) 415
BspT104I TTCGAA 1 cut(s) 493
BsrDI GCAATG 1 cut(s) 432
BssMI GATC 3 cut(s) 79, 284, 407
Bst2UI CCWGG 1 cut(s) 533
Bst4CI ACNGT 2 cut(s) 231, 437
Bst6I CTCTTC 1 cut(s) 531
BstBI TTCGAA 1 cut(s) 493
BstC8I GCNNGC 2 cut(s) 54, 114
BstDEI CTNAG 1 cut(s) 264
BstF5I GGATG 3 cut(s) 97, 316, 424
BstKTI GATC 3 cut(s) 82, 287, 410
BstMBI GATC 3 cut(s) 79, 284, 407
BstMWI GCNNNNNNNGC 9 cut(s) 53, 287, 296, 615, 624, 633, 636, 648, 660
BstNI CCWGG 1 cut(s) 533
BstPAI GACNNNNGTC 1 cut(s) 38
BstSCI CCNGG 2 cut(s) 233, 531
BstSFI CTRYAG 1 cut(s) 634
BstV1I GCAGC 3 cut(s) 638, 648, 651
BstV2I GAAGAC 1 cut(s) 102
BstX2I RGATCY 1 cut(s) 284
BstYI RGATCY 1 cut(s) 284
BsuRI GGCC 1 cut(s) 224
BtsCI GGATG 3 cut(s) 97, 316, 424
BtsIMutI CAGTG 2 cut(s) 442, 604
Cac8I GCNNGC 2 cut(s) 54, 114
Cfr13I GGNCC 3 cut(s) 70, 223, 355
Csp6I GTAC 1 cut(s) 612
CviAII CATG 2 cut(s) 359, 623
CviQI GTAC 1 cut(s) 612
DdeI CTNAG 1 cut(s) 264
DpnI GATC 3 cut(s) 81, 286, 409
DpnII GATC 3 cut(s) 79, 284, 407
DraI TTTAAA 1 cut(s) 186
Eam1104I CTCTTC 1 cut(s) 531
EarI CTCTTC 1 cut(s) 531
Eco24I GRGCYC 1 cut(s) 528
Eco47I GGWCC 2 cut(s) 70, 355
EcoO109I RGGNCCY 1 cut(s) 223
EcoRII CCWGG 1 cut(s) 531
EcoT22I ATGCAT 1 cut(s) 319
EcoT38I GRGCYC 1 cut(s) 528
FaeI CATG 2 cut(s) 362, 626
FaiI YATR 5 cut(s) 50, 360, 476, 521, 624
FaqI GGGAC 3 cut(s) 217, 224, 471
FatI CATG 2 cut(s) 358, 622
FauI CCCGC 1 cut(s) 656
Fnu4HI GCNGC 6 cut(s) 350, 628, 637, 640, 652, 655
FokI GGATG 3 cut(s) 104, 323, 431
FriOI GRGCYC 1 cut(s) 528
Fsp4HI GCNGC 6 cut(s) 350, 628, 637, 640, 652, 655
FspBI CTAG 2 cut(s) 17, 588
GluI GCNGC 6 cut(s) 350, 628, 637, 640, 652, 655
HaeIII GGCC 1 cut(s) 224
HapII CCGG 1 cut(s) 235
Hin1II CATG 2 cut(s) 362, 626
HinfI GANTC 2 cut(s) 13, 28
HpaII CCGG 1 cut(s) 235
HphI GGTGA 2 cut(s) 34, 359
Hpy188I TCNGA 5 cut(s) 33, 127, 154, 200, 454
Hpy188III TCNNGA 2 cut(s) 17, 68
HpyAV CCTTC 2 cut(s) 489, 538
HpyCH4III ACNGT 2 cut(s) 231, 437
HpyCH4V TGCA 6 cut(s) 52, 87, 317, 425, 509, 636
HpyF10VI GCNNNNNNNGC 9 cut(s) 53, 287, 296, 615, 624, 633, 636, 648, 660
HpyF3I CTNAG 1 cut(s) 264
Hsp92II CATG 2 cut(s) 362, 626
Kzo9I GATC 3 cut(s) 79, 284, 407
LmnI GCTCC 3 cut(s) 250, 523, 606
Lsp1109I GCAGC 3 cut(s) 638, 648, 651
LweI GCATC 2 cut(s) 304, 412
MaeI CTAG 2 cut(s) 17, 588
MaeIII GTNAC 1 cut(s) 10
MalI GATC 3 cut(s) 81, 286, 409
MboI GATC 3 cut(s) 79, 284, 407
MboII GAAGA 4 cut(s) 107, 178, 410, 548
MflI RGATCY 1 cut(s) 284
MhlI GDGCHC 2 cut(s) 163, 528
MluCI AATT 5 cut(s) 144, 193, 543, 570, 669
MlyI GAGTC 1 cut(s) 7
MmeI TCCRAC 2 cut(s) 223, 547
Mph1103I ATGCAT 1 cut(s) 319
MseI TTAA 4 cut(s) 143, 185, 329, 542
MspI CCGG 1 cut(s) 235
MspR9I CCNGG 2 cut(s) 235, 533
MvaI CCWGG 1 cut(s) 533
MwoI GCNNNNNNNGC 9 cut(s) 53, 287, 296, 615, 624, 633, 636, 648, 660
NciI CCSGG 1 cut(s) 235
NdeII GATC 3 cut(s) 79, 284, 407
NlaIII CATG 2 cut(s) 362, 626
NlaIV GGNNCC 3 cut(s) 225, 246, 525
NmuCI GTSAC 1 cut(s) 10
NsiI ATGCAT 1 cut(s) 319
NspV TTCGAA 1 cut(s) 493
PfeI GAWTC 1 cut(s) 28
PfoI TCCNGGA 2 cut(s) 233, 531
PkrI GCNGC 6 cut(s) 351, 629, 638, 641, 653, 656
PleI GAGTC 1 cut(s) 7
PpsI GAGTC 1 cut(s) 7
PshAI GACNNNNGTC 1 cut(s) 38
Psp6I CCWGG 1 cut(s) 531
PspGI CCWGG 1 cut(s) 531
PspN4I GGNNCC 3 cut(s) 225, 246, 525
PspPI GGNCC 3 cut(s) 70, 223, 355
PstI CTGCAG 1 cut(s) 638
PsuI RGATCY 1 cut(s) 284
RsaI GTAC 1 cut(s) 613
RsaNI GTAC 1 cut(s) 612
SaqAI TTAA 4 cut(s) 143, 185, 329, 542
SatI GCNGC 6 cut(s) 350, 628, 637, 640, 652, 655
Sau3AI GATC 3 cut(s) 79, 284, 407
Sau96I GGNCC 3 cut(s) 70, 223, 355
SchI GAGTC 1 cut(s) 7
ScrFI CCNGG 2 cut(s) 235, 533
SduI GDGCHC 2 cut(s) 163, 528
SfaNI GCATC 2 cut(s) 304, 412
SfcI CTRYAG 1 cut(s) 634
SfuI TTCGAA 1 cut(s) 493
SinI GGWCC 2 cut(s) 70, 355
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
Sse9I AATT 5 cut(s) 144, 193, 543, 570, 669
SsiI CCGC 4 cut(s) 349, 627, 654, 663
SspMI CTAG 2 cut(s) 17, 588
StyD4I CCNGG 2 cut(s) 233, 531
TaaI ACNGT 2 cut(s) 231, 437
TaqI TCGA 2 cut(s) 493, 500
TasI AATT 5 cut(s) 144, 193, 543, 570, 669
TauI GCSGC 3 cut(s) 352, 630, 657
TfiI GAWTC 1 cut(s) 28
Tru1I TTAA 4 cut(s) 143, 185, 329, 542
Tru9I TTAA 4 cut(s) 143, 185, 329, 542
TscAI CASTG 2 cut(s) 442, 604
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 3 cut(s) 636, 639, 651
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 3 cut(s) 108, 264, 459
TspRI CASTG 2 cut(s) 442, 604
VpaK11BI GGWCC 2 cut(s) 70, 355
XapI RAATTY 1 cut(s) 193
XbaI TCTAGA 1 cut(s) 16
XspI CTAG 2 cut(s) 17, 588
Zsp2I ATGCAT 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.