pycom13g12680

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0028534
Physical Location & Seq
Forward (+)
8892082 .. 8893798
1717 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 756 bp
ATGATTGTGAGCCCGGAATCGGTTCTGAGTTGCACAGAGACGATTAATAATGCTGGAATGGAGTCTTCTGCAGTCAACTTTGTGCCGAACATTCATTCAGGAAGCCATAGTGACATTGGACCAAGGGATTCCATGGATGACGAGCACATTCGGATTGATGATCTGTCTGCCCATGTTGGGGCTCTCTTCAAGTGTCCCTTTCCAAGTGCATTTTATGCGGTGTTTGATGGTCACGGAGGGCCTGAGGCAGCTGCTTATATGAAGAGGCATGCCATGAGACTATTCTTTGAAGATGCTGATTTACCGCAGAGAACGGATATGGATGCTGTTTTTTTCAGAGAGTTGGAGAATTCCCACAGGAAGGCATTTCTACAGGCAGATCAAGCCTTGTCTGAGGAACACAGTGTGAGAAGTTCGTGTGGAACAACAGCATTGACTGCTCTTATACTTGGAAGGCATTTGTTGGTTGCAAATGCTGGTGACTGTCGAGCGGTTCTTTGCAAGAAAGGAACAGCAGTTGACATGTCTCGGGATCATAAACCTAGTTACTTGCCGGAACGCATGCGAGTTGAGCAGTTGGGTGGTTTCTTTGATGATGAGTATGAGTGTCTTAATGATTCTATCAGAGTCACCCGAGCCCTTGGAGATTGGGGCTTGAAGTTACCATTAGGTTCCTTGTCGCCTCTAATTGCGGAGCCTGATGTTCAGCAGGTTATGTTAACAGAAGATGATGAGTTTTTGATCGATTGGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

252

Amino Acids

27.79

Weight (kDa)

4.81

Isoelectric Point (pI)

52.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 39 - 248 3.8e-43 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028534)

Species Orthologous Gene IDs
malus_domestica MD16G1155800.v1.1
pyrus_communis pycom13g12680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 700
AccBSI CCGCTC 1 cut(s) 491
AciI CCGC 4 cut(s) 218, 305, 491, 692
AclWI GGATC 1 cut(s) 540
AcsI RAATTY 1 cut(s) 349
AdeI CACNNNGTG 1 cut(s) 406
AfiI CCNNNNNNNGG 4 cut(s) 19, 177, 178, 361
AflIII ACRYGT 1 cut(s) 522
AgsI TTSAA 3 cut(s) 190, 290, 658
AluBI AGCT 1 cut(s) 251
AluI AGCT 1 cut(s) 251
Alw21I GWGCWC 1 cut(s) 147
Alw26I GTCTC 3 cut(s) 32, 271, 531
AlwI GGATC 1 cut(s) 540
Ama87I CYCGRG 2 cut(s) 528, 633
AoxI GGCC 1 cut(s) 239
ApeKI GCWGC 2 cut(s) 248, 251
ApoI RAATTY 1 cut(s) 349
AseI ATTAAT 1 cut(s) 45
Asp700I GAANNNNTTC 1 cut(s) 21
AspS9I GGNCC 2 cut(s) 119, 239
AsuC2I CCSGG 1 cut(s) 14
AsuHPI GGTGA 2 cut(s) 491, 622
AvaI CYCGRG 2 cut(s) 528, 633
AvaII GGWCC 1 cut(s) 119
AxyI CCTNAGG 1 cut(s) 243
BanII GRGCYC 3 cut(s) 14, 184, 640
BbsI GAAGAC 1 cut(s) 57
Bbv12I GWGCWC 1 cut(s) 147
BbvI GCAGC 2 cut(s) 238, 260
BccI CCATC 1 cut(s) 221
BcnI CCSGG 1 cut(s) 14
BcoDI GTCTC 3 cut(s) 32, 271, 531
BfaI CTAG 1 cut(s) 543
BfmI CTRYAG 2 cut(s) 69, 371
BfuAI ACCTGC 1 cut(s) 700
BisI GCNGC 2 cut(s) 249, 252
BlsI GCNGC 2 cut(s) 250, 253
Bme1390I CCNGG 1 cut(s) 14
Bme18I GGWCC 1 cut(s) 119
BmeT110I CYCGRG 2 cut(s) 528, 633
BmgT120I GGNCC 2 cut(s) 119, 239
BmiI GGNNCC 2 cut(s) 673, 696
BmrFI CCNGG 1 cut(s) 14
BmsI GCATC 2 cut(s) 283, 313
BpiI GAAGAC 1 cut(s) 57
BpuMI CCSGG 1 cut(s) 14
Bsa29I ATCGAT 1 cut(s) 744
BsaJI CCNNGG 3 cut(s) 122, 132, 640
Bsc4I CCNNNNNNNGG 4 cut(s) 19, 177, 178, 361
Bse21I CCTNAGG 1 cut(s) 243
BseCI ATCGAT 1 cut(s) 744
BseDI CCNNGG 3 cut(s) 122, 132, 640
BseGI GGATG 2 cut(s) 142, 328
BseLI CCNNNNNNNGG 4 cut(s) 19, 177, 178, 361
BseMII CTCAG 3 cut(s) 17, 234, 384
BseXI GCAGC 2 cut(s) 238, 260
BshFI GGCC 1 cut(s) 241
BshVI ATCGAT 1 cut(s) 744
BsiHKAI GWGCWC 1 cut(s) 147
BsiHKCI CYCGRG 2 cut(s) 528, 633
BsiSI CCGG 2 cut(s) 14, 554
BslFI GGGAC 1 cut(s) 180
BslI CCNNNNNNNGG 4 cut(s) 19, 177, 178, 361
BsmAI GTCTC 3 cut(s) 32, 271, 531
BsmBI CGTCTC 1 cut(s) 32
BsmFI GGGAC 1 cut(s) 180
BsnI GGCC 1 cut(s) 241
BsoBI CYCGRG 2 cut(s) 528, 633
Bsp1286I GDGCHC 4 cut(s) 14, 147, 184, 640
Bsp143I GATC 4 cut(s) 160, 379, 532, 741
Bsp19I CCATGG 1 cut(s) 132
BspACI CCGC 4 cut(s) 218, 305, 491, 692
BspANI GGCC 1 cut(s) 241
BspCNI CTCAG 3 cut(s) 18, 235, 385
BspDI ATCGAT 1 cut(s) 744
BspLI GGNNCC 2 cut(s) 673, 696
BspMAI CTGCAG 1 cut(s) 73
BspMI ACCTGC 1 cut(s) 700
BspPI GGATC 1 cut(s) 540
BsrBI CCGCTC 1 cut(s) 491
BssECI CCNNGG 3 cut(s) 122, 132, 640
BssMI GATC 4 cut(s) 160, 379, 532, 741
BssT1I CCWWGG 3 cut(s) 122, 132, 640
Bst4CI ACNGT 2 cut(s) 404, 485
Bst6I CTCTTC 2 cut(s) 191, 257
BstAPI GCANNNNNTGC 2 cut(s) 215, 437
BstC8I GCNNGC 2 cut(s) 270, 563
BstDEI CTNAG 3 cut(s) 26, 243, 393
BstDSI CCRYGG 1 cut(s) 132
BstF5I GGATG 2 cut(s) 142, 328
BstKTI GATC 4 cut(s) 163, 382, 535, 744
BstMAI GTCTC 3 cut(s) 32, 271, 531
BstMBI GATC 4 cut(s) 160, 379, 532, 741
BstMWI GCNNNNNNNGC 4 cut(s) 215, 383, 437, 571
BstNSI RCATGY 3 cut(s) 272, 526, 565
BstSCI CCNGG 1 cut(s) 12
BstSFI CTRYAG 2 cut(s) 69, 371
BstV1I GCAGC 2 cut(s) 238, 260
BstV2I GAAGAC 1 cut(s) 57
Bsu15I ATCGAT 1 cut(s) 744
Bsu36I CCTNAGG 1 cut(s) 243
BsuRI GGCC 1 cut(s) 241
BsuTUI ATCGAT 1 cut(s) 744
BtgI CCRYGG 1 cut(s) 132
BtsCI GGATG 2 cut(s) 142, 328
BtsIMutI CAGTG 1 cut(s) 409
BveI ACCTGC 1 cut(s) 700
Cac8I GCNNGC 2 cut(s) 270, 563
Cfr13I GGNCC 2 cut(s) 119, 239
ClaI ATCGAT 1 cut(s) 744
CviAII CATG 6 cut(s) 133, 173, 269, 274, 523, 562
CviJI RGCY 9 cut(s) 12, 105, 182, 241, 251, 386, 638, 654, 697
CviKI_1 RGCY 9 cut(s) 12, 105, 182, 241, 251, 386, 638, 654, 697
DdeI CTNAG 3 cut(s) 26, 243, 393
DpnI GATC 4 cut(s) 162, 381, 534, 743
DpnII GATC 4 cut(s) 160, 379, 532, 741
DraIII CACNNNGTG 1 cut(s) 406
Eam1104I CTCTTC 2 cut(s) 191, 257
EarI CTCTTC 2 cut(s) 191, 257
Eco130I CCWWGG 3 cut(s) 122, 132, 640
Eco24I GRGCYC 3 cut(s) 14, 184, 640
Eco47I GGWCC 1 cut(s) 119
Eco81I CCTNAGG 1 cut(s) 243
Eco88I CYCGRG 2 cut(s) 528, 633
EcoO109I RGGNCCY 1 cut(s) 239
EcoRI GAATTC 1 cut(s) 349
EcoT14I CCWWGG 3 cut(s) 122, 132, 640
EcoT38I GRGCYC 3 cut(s) 14, 184, 640
ErhI CCWWGG 3 cut(s) 122, 132, 640
Esp3I CGTCTC 1 cut(s) 32
FaeI CATG 6 cut(s) 136, 176, 272, 277, 526, 565
FalI AAGNNNNNCTT 2 cut(s) 182, 214
FaqI GGGAC 1 cut(s) 180
FatI CATG 6 cut(s) 132, 172, 268, 273, 522, 561
Fnu4HI GCNGC 2 cut(s) 249, 252
FokI GGATG 2 cut(s) 149, 335
FriOI GRGCYC 3 cut(s) 14, 184, 640
Fsp4HI GCNGC 2 cut(s) 249, 252
FspBI CTAG 1 cut(s) 543
GluI GCNGC 2 cut(s) 249, 252
HaeIII GGCC 1 cut(s) 241
HapII CCGG 2 cut(s) 14, 554
Hin1II CATG 6 cut(s) 136, 176, 272, 277, 526, 565
HincII GTYRAC 3 cut(s) 76, 520, 720
HindII GTYRAC 3 cut(s) 76, 520, 720
HinfI GANTC 5 cut(s) 17, 62, 128, 617, 627
HpaI GTTAAC 1 cut(s) 720
HpaII CCGG 2 cut(s) 14, 554
HphI GGTGA 2 cut(s) 491, 622
Hpy166II GTNNAC 3 cut(s) 76, 520, 720
Hpy188I TCNGA 5 cut(s) 27, 153, 338, 394, 626
Hpy188III TCNNGA 2 cut(s) 99, 530
Hpy8I GTNNAC 3 cut(s) 76, 520, 720
HpyAV CCTTC 2 cut(s) 355, 447
HpyCH4III ACNGT 2 cut(s) 404, 485
HpyCH4V TGCA 5 cut(s) 33, 71, 209, 470, 501
HpyF10VI GCNNNNNNNGC 4 cut(s) 215, 383, 437, 571
HpyF3I CTNAG 3 cut(s) 26, 243, 393
Hsp92II CATG 6 cut(s) 136, 176, 272, 277, 526, 565
KspAI GTTAAC 1 cut(s) 720
Kzo9I GATC 4 cut(s) 160, 379, 532, 741
LmnI GCTCC 1 cut(s) 694
Lsp1109I GCAGC 2 cut(s) 238, 260
LweI GCATC 2 cut(s) 283, 313
MaeI CTAG 1 cut(s) 543
MaeIII GTNAC 6 cut(s) 110, 230, 479, 545, 628, 660
MalI GATC 4 cut(s) 162, 381, 534, 743
MbiI CCGCTC 1 cut(s) 491
MboI GATC 4 cut(s) 160, 379, 532, 741
MboII GAAGA 5 cut(s) 57, 178, 274, 302, 737
MhlI GDGCHC 4 cut(s) 14, 147, 184, 640
MluCI AATT 2 cut(s) 349, 687
MlyI GAGTC 2 cut(s) 71, 636
MmeI TCCRAC 1 cut(s) 324
MnlI CCTC 5 cut(s) 230, 238, 258, 388, 693
MroXI GAANNNNTTC 1 cut(s) 21
MseI TTAA 3 cut(s) 45, 612, 719
MspA1I CMGCKG 1 cut(s) 251
MspI CCGG 2 cut(s) 14, 554
MspR9I CCNGG 1 cut(s) 14
MwoI GCNNNNNNNGC 4 cut(s) 215, 383, 437, 571
NciI CCSGG 1 cut(s) 14
NcoI CCATGG 1 cut(s) 132
NdeII GATC 4 cut(s) 160, 379, 532, 741
NlaIII CATG 6 cut(s) 136, 176, 272, 277, 526, 565
NlaIV GGNNCC 2 cut(s) 673, 696
NmuCI GTSAC 4 cut(s) 110, 230, 479, 628
NspI RCATGY 3 cut(s) 272, 526, 565
PaeI GCATGC 2 cut(s) 272, 565
PciI ACATGT 1 cut(s) 522
PdmI GAANNNNTTC 1 cut(s) 21
PfeI GAWTC 3 cut(s) 17, 128, 617
PkrI GCNGC 2 cut(s) 250, 253
PleI GAGTC 2 cut(s) 70, 635
PpsI GAGTC 2 cut(s) 70, 635
PscI ACATGT 1 cut(s) 522
PshBI ATTAAT 1 cut(s) 45
PspN4I GGNNCC 2 cut(s) 673, 696
PspPI GGNCC 2 cut(s) 119, 239
PstI CTGCAG 1 cut(s) 73
PvuII CAGCTG 1 cut(s) 251
SaqAI TTAA 3 cut(s) 45, 612, 719
SatI GCNGC 2 cut(s) 249, 252
Sau3AI GATC 4 cut(s) 160, 379, 532, 741
Sau96I GGNCC 2 cut(s) 119, 239
SchI GAGTC 2 cut(s) 71, 636
ScrFI CCNGG 1 cut(s) 14
SduI GDGCHC 4 cut(s) 14, 147, 184, 640
SetI ASST 4 cut(s) 253, 544, 673, 714
SfaNI GCATC 2 cut(s) 283, 313
SfcI CTRYAG 2 cut(s) 69, 371
SinI GGWCC 1 cut(s) 119
SphI GCATGC 2 cut(s) 272, 565
Sse9I AATT 2 cut(s) 349, 687
SsiI CCGC 4 cut(s) 218, 305, 491, 692
SspMI CTAG 1 cut(s) 543
StyD4I CCNGG 1 cut(s) 12
StyI CCWWGG 3 cut(s) 122, 132, 640
TaaI ACNGT 2 cut(s) 404, 485
TaqI TCGA 2 cut(s) 487, 744
TasI AATT 2 cut(s) 349, 687
TfiI GAWTC 3 cut(s) 17, 128, 617
Tru1I TTAA 3 cut(s) 45, 612, 719
Tru9I TTAA 3 cut(s) 45, 612, 719
TscAI CASTG 1 cut(s) 409
TseFI GTSAC 4 cut(s) 110, 230, 479, 628
TseI GCWGC 2 cut(s) 248, 251
Tsp45I GTSAC 4 cut(s) 110, 230, 479, 628
TspDTI ATGAA 2 cut(s) 83, 275
TspGWI ACGGA 2 cut(s) 249, 329
TspRI CASTG 1 cut(s) 409
VpaK11BI GGWCC 1 cut(s) 119
VspI ATTAAT 1 cut(s) 45
XapI RAATTY 1 cut(s) 349
XceI RCATGY 3 cut(s) 272, 526, 565
XcmI CCANNNNNNNNNTGG 1 cut(s) 113
XmnI GAANNNNTTC 1 cut(s) 21
XspI CTAG 1 cut(s) 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.