pycom13g20380

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0029445
Physical Location & Seq
Forward (+)
16453924 .. 16455591
1668 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g20380.1

Sequence Viewer

Length: 768 bp
ATGCGAATCGATTCGCAGCTCAAGTCACTTAACAAACGAACCCAACACGATATGATCAAACTTACTCACATTCCTCATCATCCCCTGCTAGAGGTTTCAACCTCCATCTTCAATCCCATGCCGAACTGCATACCATGGATCACTCTGATCATTTTCTTGACTGTAACAGAAATGGCGGTGGCACTGACGCAGTCTCCCATTCCCACCACTCTAGAAGGCCCGTTCGTGCCTGCAACTCGGCGCTTCGATCCATCGCTTCGCAGAGGCAGTGATGACTTGCCAATGGACCATCCCAGGCTCAAGAAGAACGTGACATCAAATTTCCCAGAACAGATTGCCCTTGCTATTTCTTCACCCACTGCAATGTGGGTTTCTTGGGTTACTGGGGATGCCCAGATTGGTAAAACTGTGAGACCACTTGATCCTTCAACAGTTGGGAGTGAGGTGAGGTATGGGGAAGAAAGTGGCAAGTATACGAGTGTGAAGAAAGGGGTTTCAGTTGTTTACAGTCAATTGTACCCTTTTGCTGGTCTCTTGAATTACACCTCTGCCATCATTCATCATGTCAGGATTGATGGTCTTAAACCAGGTATGAAATATTATTACAAGTGTGGGGATAGTTCTATCCCTGCTATGAGTCAAGAGCAAGCCCTTGAAACTTTACCAGAGCCTGGCGAAAACACATATCCTCGTCGAATAGCTGTTGTTGGAGATTTGGGTCTCACTAGCAATACCAGTACAACCATTGATCGATTTAATCCAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

256

Amino Acids

28.32

Weight (kDa)

7.8

Isoelectric Point (pI)

42.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pur_ac_phosph_N PF16656 109 - 214 5.7e-17 Purple acid Phosphatase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029445)

Species Orthologous Gene IDs
pyrus_communis pycom13g20380 pycom16g19780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 671
AccI GTMKAC 1 cut(s) 473
AciI CCGC 1 cut(s) 176
AclWI GGATC 3 cut(s) 146, 242, 416
AcsI RAATTY 1 cut(s) 319
AfaI GTAC 2 cut(s) 518, 739
AfiI CCNNNNNNNGG 3 cut(s) 91, 527, 671
AgsI TTSAA 5 cut(s) 99, 112, 429, 538, 656
AjnI CCWGG 3 cut(s) 293, 586, 670
AluBI AGCT 2 cut(s) 19, 701
AluI AGCT 2 cut(s) 19, 701
Alw26I GTCTC 4 cut(s) 198, 406, 536, 725
AlwI GGATC 3 cut(s) 146, 242, 416
AlwNI CAGNNNCTG 1 cut(s) 671
AoxI GGCC 1 cut(s) 217
ApeKI GCWGC 1 cut(s) 16
ApoI RAATTY 1 cut(s) 319
Asp700I GAANNNNTTC 1 cut(s) 10
AspLEI GCGC 1 cut(s) 243
AspS9I GGNCC 2 cut(s) 218, 286
AsuHPI GGTGA 2 cut(s) 345, 457
AvaII GGWCC 1 cut(s) 286
BbvI GCAGC 1 cut(s) 28
BccI CCATC 5 cut(s) 113, 259, 297, 560, 569
BciT130I CCWGG 3 cut(s) 295, 588, 672
BclI TGATCA 2 cut(s) 54, 147
BcoDI GTCTC 4 cut(s) 198, 406, 536, 725
BfaI CTAG 3 cut(s) 89, 212, 726
BfoI RGCGCY 1 cut(s) 244
BisI GCNGC 1 cut(s) 17
BlsI GCNGC 1 cut(s) 18
Bme1390I CCNGG 3 cut(s) 295, 588, 672
Bme18I GGWCC 1 cut(s) 286
BmgT120I GGNCC 2 cut(s) 218, 286
BmrFI CCNGG 3 cut(s) 295, 588, 672
BmrI ACTGGG 1 cut(s) 393
BmsI GCATC 1 cut(s) 379
BmuI ACTGGG 1 cut(s) 393
BpuEI CTTGAG 2 cut(s) 5, 284
Bsa29I ATCGAT 2 cut(s) 9, 751
BsaI GGTCTC 3 cut(s) 406, 536, 725
BsaJI CCNNGG 2 cut(s) 134, 293
BsaXI ACNNNNNCTCC 2 cut(s) 178, 208
Bsc4I CCNNNNNNNGG 3 cut(s) 91, 527, 671
Bse1I ACTGG 2 cut(s) 388, 735
Bse3DI GCAATG 1 cut(s) 369
BseBI CCWGG 3 cut(s) 295, 588, 672
BseCI ATCGAT 2 cut(s) 9, 751
BseDI CCNNGG 2 cut(s) 134, 293
BseGI GGATG 3 cut(s) 79, 289, 394
BseLI CCNNNNNNNGG 3 cut(s) 91, 527, 671
BseMI GCAATG 1 cut(s) 369
BseNI ACTGG 2 cut(s) 388, 735
BseXI GCAGC 1 cut(s) 28
BshFI GGCC 1 cut(s) 219
BshVI ATCGAT 2 cut(s) 9, 751
BslI CCNNNNNNNGG 3 cut(s) 91, 527, 671
BsmAI GTCTC 4 cut(s) 198, 406, 536, 725
BsnI GGCC 1 cut(s) 219
Bso31I GGTCTC 3 cut(s) 406, 536, 725
Bsp143I GATC 6 cut(s) 54, 138, 147, 247, 421, 748
Bsp19I CCATGG 1 cut(s) 134
BspACI CCGC 1 cut(s) 176
BspANI GGCC 1 cut(s) 219
BspDI ATCGAT 2 cut(s) 9, 751
BspPI GGATC 3 cut(s) 146, 242, 416
BspTNI GGTCTC 3 cut(s) 406, 536, 725
BsrDI GCAATG 1 cut(s) 369
BsrI ACTGG 2 cut(s) 388, 735
BssECI CCNNGG 2 cut(s) 134, 293
BssMI GATC 6 cut(s) 54, 138, 147, 247, 421, 748
BssNAI GTATAC 1 cut(s) 474
BssT1I CCWWGG 1 cut(s) 134
Bst1107I GTATAC 1 cut(s) 474
Bst2UI CCWGG 3 cut(s) 295, 588, 672
Bst4CI ACNGT 4 cut(s) 163, 409, 433, 509
BstC8I GCNNGC 2 cut(s) 231, 648
BstDSI CCRYGG 1 cut(s) 134
BstENI CCTNNNNNAGG 1 cut(s) 89
BstF5I GGATG 3 cut(s) 79, 289, 394
BstH2I RGCGCY 1 cut(s) 244
BstHHI GCGC 1 cut(s) 243
BstKTI GATC 6 cut(s) 57, 141, 150, 250, 424, 751
BstMAI GTCTC 4 cut(s) 198, 406, 536, 725
BstMBI GATC 6 cut(s) 54, 138, 147, 247, 421, 748
BstNI CCWGG 3 cut(s) 295, 588, 672
BstSCI CCNGG 3 cut(s) 293, 586, 670
BstV1I GCAGC 1 cut(s) 28
BstZ17I GTATAC 1 cut(s) 474
Bsu15I ATCGAT 2 cut(s) 9, 751
BsuRI GGCC 1 cut(s) 219
BsuTUI ATCGAT 2 cut(s) 9, 751
BtgI CCRYGG 1 cut(s) 134
BtgZI GCGATG 1 cut(s) 237
BtsCI GGATG 3 cut(s) 79, 289, 394
BtsI GCAGTG 2 cut(s) 274, 357
BtsIMutI CAGTG 3 cut(s) 182, 274, 357
Cac8I GCNNGC 2 cut(s) 231, 648
CaiI CAGNNNCTG 1 cut(s) 671
CfoI GCGC 1 cut(s) 243
Cfr13I GGNCC 2 cut(s) 218, 286
ClaI ATCGAT 2 cut(s) 9, 751
CseI GACGC 1 cut(s) 196
CsiI ACCWGGT 1 cut(s) 586
Csp6I GTAC 2 cut(s) 517, 738
CviAII CATG 3 cut(s) 118, 135, 563
CviJI RGCY 6 cut(s) 19, 219, 298, 650, 670, 701
CviKI_1 RGCY 6 cut(s) 19, 219, 298, 650, 670, 701
CviQI GTAC 2 cut(s) 517, 738
DpnI GATC 6 cut(s) 56, 140, 149, 249, 423, 750
DpnII GATC 6 cut(s) 54, 138, 147, 247, 421, 748
Eco130I CCWWGG 1 cut(s) 134
Eco31I GGTCTC 3 cut(s) 406, 536, 725
Eco47I GGWCC 1 cut(s) 286
EcoNI CCTNNNNNAGG 1 cut(s) 89
EcoRII CCWGG 3 cut(s) 293, 586, 670
EcoT14I CCWWGG 1 cut(s) 134
ErhI CCWWGG 1 cut(s) 134
FaeI CATG 3 cut(s) 121, 138, 566
FatI CATG 3 cut(s) 117, 134, 562
FbaI TGATCA 2 cut(s) 54, 147
FblI GTMKAC 1 cut(s) 473
Fnu4HI GCNGC 1 cut(s) 17
FokI GGATG 3 cut(s) 66, 276, 401
Fsp4HI GCNGC 1 cut(s) 17
FspBI CTAG 3 cut(s) 89, 212, 726
GlaI GCGC 1 cut(s) 242
GluI GCNGC 1 cut(s) 17
HaeII RGCGCY 1 cut(s) 244
HaeIII GGCC 1 cut(s) 219
HgaI GACGC 1 cut(s) 196
HhaI GCGC 1 cut(s) 243
Hin1II CATG 3 cut(s) 121, 138, 566
Hin6I GCGC 1 cut(s) 241
HinP1I GCGC 1 cut(s) 241
HinfI GANTC 3 cut(s) 6, 11, 637
HphI GGTGA 2 cut(s) 345, 457
Hpy166II GTNNAC 2 cut(s) 474, 505
Hpy188I TCNGA 1 cut(s) 147
Hpy188III TCNNGA 7 cut(s) 157, 212, 301, 535, 568, 641, 761
Hpy8I GTNNAC 2 cut(s) 474, 505
Hpy99I CGWCG 1 cut(s) 696
HpyAV CCTTC 2 cut(s) 209, 435
HpyCH4III ACNGT 4 cut(s) 163, 409, 433, 509
HpyCH4IV ACGT 1 cut(s) 309
HpyCH4V TGCA 3 cut(s) 129, 233, 362
HpySE526I ACGT 1 cut(s) 309
Hsp92II CATG 3 cut(s) 121, 138, 566
HspAI GCGC 1 cut(s) 241
Ksp22I TGATCA 2 cut(s) 54, 147
Kzo9I GATC 6 cut(s) 54, 138, 147, 247, 421, 748
Lsp1109I GCAGC 1 cut(s) 28
LweI GCATC 1 cut(s) 379
MabI ACCWGGT 1 cut(s) 586
MaeI CTAG 3 cut(s) 89, 212, 726
MaeII ACGT 1 cut(s) 309
MaeIII GTNAC 4 cut(s) 24, 163, 310, 379
MalI GATC 6 cut(s) 56, 140, 149, 249, 423, 750
MboI GATC 6 cut(s) 54, 138, 147, 247, 421, 748
MboII GAAGA 5 cut(s) 100, 316, 342, 470, 496
MfeI CAATTG 1 cut(s) 512
MluCI AATT 3 cut(s) 319, 512, 538
MlyI GAGTC 1 cut(s) 646
MmeI TCCRAC 1 cut(s) 688
MnlI CCTC 8 cut(s) 84, 85, 112, 257, 436, 441, 556, 699
MroXI GAANNNNTTC 1 cut(s) 10
MseI TTAA 3 cut(s) 30, 582, 756
MslI CAYNNNNRTG 1 cut(s) 362
MspR9I CCNGG 3 cut(s) 295, 588, 672
MunI CAATTG 1 cut(s) 512
MvaI CCWGG 3 cut(s) 295, 588, 672
NcoI CCATGG 1 cut(s) 134
NdeII GATC 6 cut(s) 54, 138, 147, 247, 421, 748
NlaIII CATG 3 cut(s) 121, 138, 566
NmeAIII GCCGAG 1 cut(s) 217
NmuCI GTSAC 2 cut(s) 24, 310
PdmI GAANNNNTTC 1 cut(s) 10
PfeI GAWTC 2 cut(s) 6, 11
PflFI GACNNNGTC 1 cut(s) 190
PflMI CCANNNNNTGG 1 cut(s) 671
PkrI GCNGC 1 cut(s) 18
PleI GAGTC 1 cut(s) 645
PpsI GAGTC 1 cut(s) 645
Psp6I CCWGG 3 cut(s) 293, 586, 670
PspGI CCWGG 3 cut(s) 293, 586, 670
PspPI GGNCC 2 cut(s) 218, 286
PstNI CAGNNNCTG 1 cut(s) 671
PsyI GACNNNGTC 1 cut(s) 190
RsaI GTAC 2 cut(s) 518, 739
RsaNI GTAC 2 cut(s) 517, 738
RseI CAYNNNNRTG 1 cut(s) 362
SaqAI TTAA 3 cut(s) 30, 582, 756
SatI GCNGC 1 cut(s) 17
Sau3AI GATC 6 cut(s) 54, 138, 147, 247, 421, 748
Sau96I GGNCC 2 cut(s) 218, 286
SchI GAGTC 1 cut(s) 646
ScrFI CCNGG 3 cut(s) 295, 588, 672
SetI ASST 9 cut(s) 21, 96, 104, 312, 447, 452, 548, 592, 703
SexAI ACCWGGT 1 cut(s) 586
SfaNI GCATC 1 cut(s) 379
SinI GGWCC 1 cut(s) 286
SmiMI CAYNNNNRTG 1 cut(s) 362
SmlI CTYRAG 2 cut(s) 20, 299
SmoI CTYRAG 2 cut(s) 20, 299
Sse9I AATT 3 cut(s) 319, 512, 538
SsiI CCGC 1 cut(s) 176
SspI AATATT 1 cut(s) 599
SspMI CTAG 3 cut(s) 89, 212, 726
StyD4I CCNGG 3 cut(s) 293, 586, 670
StyI CCWWGG 1 cut(s) 134
TaaI ACNGT 4 cut(s) 163, 409, 433, 509
TaiI ACGT 1 cut(s) 312
TaqI TCGA 4 cut(s) 9, 246, 694, 751
TasI AATT 3 cut(s) 319, 512, 538
TatI WGTACW 1 cut(s) 737
TfiI GAWTC 2 cut(s) 6, 11
Tru1I TTAA 3 cut(s) 30, 582, 756
Tru9I TTAA 3 cut(s) 30, 582, 756
TscAI CASTG 3 cut(s) 189, 274, 364
TseFI GTSAC 2 cut(s) 24, 310
TseI GCWGC 1 cut(s) 16
Tsp45I GTSAC 2 cut(s) 24, 310
TspDTI ATGAA 2 cut(s) 548, 608
TspRI CASTG 3 cut(s) 189, 274, 364
Tth111I GACNNNGTC 1 cut(s) 190
Van91I CCANNNNNTGG 1 cut(s) 671
VpaK11BI GGWCC 1 cut(s) 286
XagI CCTNNNNNAGG 1 cut(s) 89
XapI RAATTY 1 cut(s) 319
XbaI TCTAGA 1 cut(s) 211
XmiI GTMKAC 1 cut(s) 473
XmnI GAANNNNTTC 1 cut(s) 10
XspI CTAG 3 cut(s) 89, 212, 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.