pycom13g26740

AAA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0026678
Physical Location & Seq
Forward (+)
23264799 .. 23265759
961 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g26740.3

Sequence Viewer

Length: 633 bp
ATGCTAACACGTCTGAATTTTAGTCGTTTAATTTGTTGTACGTTATGTGTCTACGTATACACACGCAGGCATTGTCTTTGGATATTGTGGAACGAACCAACTTTGGTTAGGAGTGAGAGTATTTGGAAGAAGGTAATTGTTGATGCTAAGAAACGCAAATGTTTCTATAATGCTGAGGAAGACAAGGACTTGTCTCAGGCTTTGGCAGCTGCCCTTGTGGATTCGAGCAGTTTCCGGGAAGTTGATCCCGCGGAGTTCCTTTCAAAACCGTTATCTTCTCTCAGTCTAACAGAAGAGCCACAGACATCATCAAGTAGTTATAGACGAGTGGTTCATATTACTTGGTTTGGTGAGATTATAATAATATCTAATCCACTTATCCCACTACAACCGACACCGGTAAATGGTAACAACGGTAATCGCTCCGATTCAATAATTAGTGACCTCATTAAAAAACATCGTAGCCAACACGTTGGACTCTCTAATGCTGAAAAAGAGAGTGAAATAGCATACTTTATGGTTGCTCCCCCTGATAATTGCAAGGTAGACTTTGATCAGGGTCGGGTAAAAAGGTCCATGCACTACGGTACCATTGAAACAGATTTGTTAAACTTCAATTTCATGACTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12

Protein Analysis

211

Amino Acids

24.05

Weight (kDa)

7.62

Isoelectric Point (pI)

59.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0026678)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37030
pyrus_communis pycom13g26740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 359
Acc65I GGTACC 1 cut(s) 587
AccB1I GGYRCC 1 cut(s) 587
AccI GTMKAC 3 cut(s) 51, 57, 546
AccII CGCG 1 cut(s) 251
AciI CCGC 2 cut(s) 249, 251
AclWI GGATC 1 cut(s) 239
AcsI RAATTY 1 cut(s) 16
AfaI GTAC 2 cut(s) 40, 589
AfiI CCNNNNNNNGG 1 cut(s) 404
AflIII ACRYGT 2 cut(s) 8, 469
AgeI ACCGGT 1 cut(s) 397
AgsI TTSAA 4 cut(s) 264, 432, 596, 616
AjiI CACGTC 1 cut(s) 11
AluBI AGCT 1 cut(s) 209
AluI AGCT 1 cut(s) 209
Alw26I GTCTC 1 cut(s) 198
AlwI GGATC 1 cut(s) 239
ApeKI GCWGC 2 cut(s) 206, 209
ApoI RAATTY 1 cut(s) 16
AsiGI ACCGGT 1 cut(s) 397
Asp718I GGTACC 1 cut(s) 587
AspS9I GGNCC 1 cut(s) 573
AsuC2I CCSGG 1 cut(s) 236
AsuHPI GGTGA 1 cut(s) 362
AvaII GGWCC 1 cut(s) 573
BaeI ACNNNNGTAYC 2 cut(s) 571, 604
BanI GGYRCC 1 cut(s) 587
BbsI GAAGAC 1 cut(s) 186
BbvCI CCTCAGC 1 cut(s) 174
BbvI GCAGC 2 cut(s) 196, 218
BclI TGATCA 1 cut(s) 553
BcnI CCSGG 1 cut(s) 236
BcoDI GTCTC 1 cut(s) 198
BisI GCNGC 2 cut(s) 207, 210
BlsI GCNGC 2 cut(s) 208, 211
Bme1390I CCNGG 1 cut(s) 236
Bme18I GGWCC 1 cut(s) 573
BmgBI CACGTC 1 cut(s) 11
BmgT120I GGNCC 1 cut(s) 573
BmiI GGNNCC 1 cut(s) 589
BmrFI CCNGG 1 cut(s) 236
BmsI GCATC 1 cut(s) 133
BpiI GAAGAC 1 cut(s) 186
Bpu10I CCTNAGC 1 cut(s) 174
BpuMI CCSGG 1 cut(s) 236
BsaAI YACGTR 1 cut(s) 55
BsaJI CCNNGG 1 cut(s) 249
BsaWI WCCGGW 1 cut(s) 397
BsaXI ACNNNNNCTCC 2 cut(s) 103, 133
Bsc4I CCNNNNNNNGG 1 cut(s) 404
Bse118I RCCGGY 1 cut(s) 397
BseDI CCNNGG 1 cut(s) 249
BseLI CCNNNNNNNGG 1 cut(s) 404
BseMII CTCAG 3 cut(s) 165, 209, 295
BseXI GCAGC 2 cut(s) 196, 218
Bsh1236I CGCG 1 cut(s) 251
BshNI GGYRCC 1 cut(s) 587
BshTI ACCGGT 1 cut(s) 397
BsiSI CCGG 2 cut(s) 235, 398
BslI CCNNNNNNNGG 1 cut(s) 404
BsmAI GTCTC 1 cut(s) 198
Bsp143I GATC 2 cut(s) 244, 553
BspACI CCGC 2 cut(s) 249, 251
BspCNI CTCAG 3 cut(s) 166, 208, 294
BspFNI CGCG 1 cut(s) 251
BspHI TCATGA 1 cut(s) 621
BspLI GGNNCC 1 cut(s) 589
BspPI GGATC 1 cut(s) 239
BspQI GCTCTTC 1 cut(s) 288
BspT107I GGYRCC 1 cut(s) 587
BsrFI RCCGGY 1 cut(s) 397
BssAI RCCGGY 1 cut(s) 397
BssECI CCNNGG 1 cut(s) 249
BssMI GATC 2 cut(s) 244, 553
BssNAI GTATAC 1 cut(s) 58
Bst1107I GTATAC 1 cut(s) 58
Bst4CI ACNGT 3 cut(s) 270, 416, 587
Bst6I CTCTTC 1 cut(s) 288
BstBAI YACGTR 1 cut(s) 55
BstC8I GCNNGC 1 cut(s) 68
BstDEI CTNAG 4 cut(s) 147, 174, 195, 281
BstDSI CCRYGG 1 cut(s) 249
BstFNI CGCG 1 cut(s) 251
BstKTI GATC 2 cut(s) 247, 556
BstMAI GTCTC 1 cut(s) 198
BstMBI GATC 2 cut(s) 244, 553
BstMWI GCNNNNNNNGC 1 cut(s) 206
BstSCI CCNGG 1 cut(s) 234
BstSNI TACGTA 1 cut(s) 55
BstUI CGCG 1 cut(s) 251
BstV1I GCAGC 2 cut(s) 196, 218
BstV2I GAAGAC 1 cut(s) 186
BstXI CCANNNNNNTGG 1 cut(s) 473
BstZ17I GTATAC 1 cut(s) 58
BtgI CCRYGG 1 cut(s) 249
BtrI CACGTC 1 cut(s) 11
Cac8I GCNNGC 1 cut(s) 68
CciI TCATGA 1 cut(s) 621
Cfr10I RCCGGY 1 cut(s) 397
Cfr13I GGNCC 1 cut(s) 573
Cfr42I CCGCGG 1 cut(s) 252
Csp6I GTAC 2 cut(s) 39, 588
CspAI ACCGGT 1 cut(s) 397
CviAII CATG 2 cut(s) 577, 622
CviJI RGCY 4 cut(s) 200, 209, 298, 465
CviKI_1 RGCY 4 cut(s) 200, 209, 298, 465
CviQI GTAC 2 cut(s) 39, 588
DdeI CTNAG 4 cut(s) 147, 174, 195, 281
DpnI GATC 2 cut(s) 246, 555
DpnII GATC 2 cut(s) 244, 553
Eam1104I CTCTTC 1 cut(s) 288
EarI CTCTTC 1 cut(s) 288
Eco105I TACGTA 1 cut(s) 55
Eco47I GGWCC 1 cut(s) 573
FaeI CATG 2 cut(s) 580, 625
FalI AAGNNNNNCTT 2 cut(s) 533, 565
FatI CATG 2 cut(s) 576, 621
FauI CCCGC 1 cut(s) 256
FbaI TGATCA 1 cut(s) 553
FblI GTMKAC 3 cut(s) 51, 57, 546
Fnu4HI GCNGC 2 cut(s) 207, 210
Fsp4HI GCNGC 2 cut(s) 207, 210
GluI GCNGC 2 cut(s) 207, 210
HapII CCGG 2 cut(s) 235, 398
Hin1II CATG 2 cut(s) 580, 625
HinfI GANTC 3 cut(s) 221, 428, 477
HpaII CCGG 2 cut(s) 235, 398
HphI GGTGA 1 cut(s) 362
Hpy166II GTNNAC 3 cut(s) 52, 58, 547
Hpy188I TCNGA 3 cut(s) 15, 427, 632
Hpy188III TCNNGA 1 cut(s) 622
Hpy8I GTNNAC 3 cut(s) 52, 58, 547
HpyAV CCTTC 1 cut(s) 124
HpyCH4III ACNGT 3 cut(s) 270, 416, 587
HpyCH4IV ACGT 4 cut(s) 10, 41, 54, 471
HpyCH4V TGCA 2 cut(s) 540, 580
HpyF10VI GCNNNNNNNGC 1 cut(s) 206
HpyF3I CTNAG 4 cut(s) 147, 174, 195, 281
HpySE526I ACGT 4 cut(s) 10, 41, 54, 471
Hsp92II CATG 2 cut(s) 580, 625
KpnI GGTACC 1 cut(s) 591
Ksp22I TGATCA 1 cut(s) 553
KspI CCGCGG 1 cut(s) 252
Kzo9I GATC 2 cut(s) 244, 553
LguI GCTCTTC 1 cut(s) 288
LmnI GCTCC 2 cut(s) 428, 529
LpnPI CCDG 6 cut(s) 52, 182, 248, 411, 542, 543
Lsp1109I GCAGC 2 cut(s) 196, 218
LweI GCATC 1 cut(s) 133
MaeII ACGT 4 cut(s) 10, 41, 54, 471
MaeIII GTNAC 2 cut(s) 407, 440
MalI GATC 2 cut(s) 246, 555
MboI GATC 2 cut(s) 244, 553
MboII GAAGA 4 cut(s) 139, 191, 267, 305
MluCI AATT 6 cut(s) 16, 30, 135, 435, 535, 616
MlyI GAGTC 1 cut(s) 471
MmeI TCCRAC 1 cut(s) 454
MnlI CCTC 2 cut(s) 169, 455
MseI TTAA 3 cut(s) 29, 450, 608
MspA1I CMGCKG 2 cut(s) 209, 251
MspI CCGG 2 cut(s) 235, 398
MspR9I CCNGG 1 cut(s) 236
MvnI CGCG 1 cut(s) 251
MwoI GCNNNNNNNGC 1 cut(s) 206
NciI CCSGG 1 cut(s) 236
NdeII GATC 2 cut(s) 244, 553
NlaIII CATG 2 cut(s) 580, 625
NlaIV GGNNCC 1 cut(s) 589
NmuCI GTSAC 1 cut(s) 440
PagI TCATGA 1 cut(s) 621
PciSI GCTCTTC 1 cut(s) 288
PfeI GAWTC 2 cut(s) 221, 428
PfoI TCCNGGA 1 cut(s) 234
PinAI ACCGGT 1 cut(s) 397
PkrI GCNGC 2 cut(s) 208, 211
PleI GAGTC 1 cut(s) 471
PpsI GAGTC 1 cut(s) 471
Ppu21I YACGTR 1 cut(s) 55
PsiI TTATAA 1 cut(s) 359
PspN4I GGNNCC 1 cut(s) 589
PspPI GGNCC 1 cut(s) 573
PvuII CAGCTG 1 cut(s) 209
RsaI GTAC 2 cut(s) 40, 589
RsaNI GTAC 2 cut(s) 39, 588
SacII CCGCGG 1 cut(s) 252
SapI GCTCTTC 1 cut(s) 288
SaqAI TTAA 3 cut(s) 29, 450, 608
SatI GCNGC 2 cut(s) 207, 210
Sau3AI GATC 2 cut(s) 244, 553
Sau96I GGNCC 1 cut(s) 573
SchI GAGTC 1 cut(s) 471
ScrFI CCNGG 1 cut(s) 236
SetI ASST 9 cut(s) 13, 44, 57, 135, 211, 447, 474, 546, 575
SfaNI GCATC 1 cut(s) 133
Sfr303I CCGCGG 1 cut(s) 252
SgrBI CCGCGG 1 cut(s) 252
SinI GGWCC 1 cut(s) 573
SnaBI TACGTA 1 cut(s) 55
Sse9I AATT 6 cut(s) 16, 30, 135, 435, 535, 616
SsiI CCGC 2 cut(s) 249, 251
StyD4I CCNGG 1 cut(s) 234
TaaI ACNGT 3 cut(s) 270, 416, 587
TaiI ACGT 4 cut(s) 13, 44, 57, 474
TaqI TCGA 1 cut(s) 224
TasI AATT 6 cut(s) 16, 30, 135, 435, 535, 616
TfiI GAWTC 2 cut(s) 221, 428
Tru1I TTAA 3 cut(s) 29, 450, 608
Tru9I TTAA 3 cut(s) 29, 450, 608
TseFI GTSAC 1 cut(s) 440
TseI GCWGC 2 cut(s) 206, 209
Tsp45I GTSAC 1 cut(s) 440
TspDTI ATGAA 2 cut(s) 323, 610
VpaK11BI GGWCC 1 cut(s) 573
XapI RAATTY 1 cut(s) 16
XmiI GTMKAC 3 cut(s) 51, 57, 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.