pycom13g28250

Component of the EKC KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0029449
Physical Location & Seq
Forward (+)
24177873 .. 24179591
1719 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 384 bp
ATGGGAGCACCGCTACAAGTTGCTGCAATTGTTGTCAGGGTTCTTTCGCAATTGTGGAAGAAGCCCATTGTTGCAGTTAATCATTGTATTGCACATATTGAGATGGGAAGTGTTGTGACCGGTGCTGATGATCCTGTTGTCTTATATGTTAGTGCTGGGAACACACAGCTTGCAAAGAAAGGAGAACATTTTATAGATCTTCCTTATGTTGTCAAAGGAATGGATGTATCTTTTAGTGGAATTTTGAGCTATATTGAAGCTACCGCTGTGGAGAAGCTCAAGAATAATGAGTGCACATCTGCAGACCTATGCTACTCCCTTCAGATGAGAAAATGTGTTTGCAATGCTTGTGGAGATAACGGAACGTGCAATGGCACAATGTGA

Protein Analysis

128

Amino Acids

13.55

Weight (kDa)

6.25

Isoelectric Point (pI)

24.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TsaD PF00814 2 - 57 8.5e-13 tRNA N6-adenosine threonylcarbamoyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0029449)

Species Orthologous Gene IDs
pyrus_communis pycom13g28250
rosa_chinensis RchiOBHm_Chr1g0329841

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 11, 264
AclWI GGATC 1 cut(s) 125
AcsI RAATTY 1 cut(s) 240
AcuI CTGAAG 1 cut(s) 305
AdeI CACNNNGTG 1 cut(s) 381
AgeI ACCGGT 1 cut(s) 119
AgsI TTSAA 1 cut(s) 257
AluBI AGCT 4 cut(s) 169, 249, 260, 277
AluI AGCT 4 cut(s) 169, 249, 260, 277
Alw21I GWGCWC 2 cut(s) 10, 296
Alw44I GTGCAC 1 cut(s) 292
AlwI GGATC 1 cut(s) 125
ApaLI GTGCAC 1 cut(s) 292
ApeKI GCWGC 1 cut(s) 23
ApoI RAATTY 1 cut(s) 240
AsiGI ACCGGT 1 cut(s) 119
BaeGI GKGCMC 1 cut(s) 296
Bbv12I GWGCWC 2 cut(s) 10, 296
BbvI GCAGC 1 cut(s) 10
BccI CCATC 1 cut(s) 97
BfmI CTRYAG 1 cut(s) 300
BglII AGATCT 1 cut(s) 196
BisI GCNGC 1 cut(s) 24
BlsI GCNGC 1 cut(s) 25
BpuEI CTTGAG 1 cut(s) 263
BsaWI WCCGGW 1 cut(s) 119
Bse118I RCCGGY 1 cut(s) 119
Bse3DI GCAATG 2 cut(s) 349, 376
BseGI GGATG 1 cut(s) 229
BseMI GCAATG 2 cut(s) 349, 376
BseSI GKGCMC 1 cut(s) 296
BseXI GCAGC 1 cut(s) 10
BseYI CCCAGC 1 cut(s) 155
BshTI ACCGGT 1 cut(s) 119
BsiHKAI GWGCWC 2 cut(s) 10, 296
BsiSI CCGG 1 cut(s) 120
Bsp1286I GDGCHC 2 cut(s) 10, 296
Bsp143I GATC 2 cut(s) 130, 196
BspACI CCGC 2 cut(s) 11, 264
BspMAI CTGCAG 1 cut(s) 304
BspPI GGATC 1 cut(s) 125
BsrDI GCAATG 2 cut(s) 349, 376
BsrFI RCCGGY 1 cut(s) 119
BssAI RCCGGY 1 cut(s) 119
BssMI GATC 2 cut(s) 130, 196
BstC8I GCNNGC 1 cut(s) 171
BstF5I GGATG 1 cut(s) 229
BstKTI GATC 2 cut(s) 133, 199
BstMBI GATC 2 cut(s) 130, 196
BstSFI CTRYAG 1 cut(s) 300
BstSLI GKGCMC 1 cut(s) 296
BstV1I GCAGC 1 cut(s) 10
BstX2I RGATCY 1 cut(s) 196
BstYI RGATCY 1 cut(s) 196
BtsCI GGATG 1 cut(s) 229
Cac8I GCNNGC 1 cut(s) 171
Cfr10I RCCGGY 1 cut(s) 119
CspAI ACCGGT 1 cut(s) 119
CspCI CAANNNNNGTGG 2 cut(s) 331, 366
CviJI RGCY 5 cut(s) 64, 169, 249, 260, 277
CviKI_1 RGCY 5 cut(s) 64, 169, 249, 260, 277
DpnI GATC 2 cut(s) 132, 198
DpnII GATC 2 cut(s) 130, 196
DraIII CACNNNGTG 1 cut(s) 381
Eco57I CTGAAG 1 cut(s) 305
FaiI YATR 7 cut(s) 96, 145, 147, 194, 207, 252, 310
Fnu4HI GCNGC 1 cut(s) 24
FokI GGATG 1 cut(s) 236
Fsp4HI GCNGC 1 cut(s) 24
GluI GCNGC 1 cut(s) 24
GsaI CCCAGC 1 cut(s) 159
HapII CCGG 1 cut(s) 120
HpaII CCGG 1 cut(s) 120
Hpy166II GTNNAC 1 cut(s) 294
Hpy188I TCNGA 1 cut(s) 324
Hpy188III TCNNGA 1 cut(s) 280
Hpy8I GTNNAC 1 cut(s) 294
HpyAV CCTTC 1 cut(s) 329
HpyCH4IV ACGT 1 cut(s) 365
HpyCH4V TGCA 8 cut(s) 26, 74, 92, 173, 294, 302, 342, 369
HpySE526I ACGT 1 cut(s) 365
Kzo9I GATC 2 cut(s) 130, 196
LmnI GCTCC 1 cut(s) 5
LpnPI CCDG 4 cut(s) 22, 133, 141, 147
Lsp1109I GCAGC 1 cut(s) 10
MaeII ACGT 1 cut(s) 365
MaeIII GTNAC 1 cut(s) 115
MalI GATC 2 cut(s) 132, 198
MboI GATC 2 cut(s) 130, 196
MboII GAAGA 2 cut(s) 70, 191
MfeI CAATTG 2 cut(s) 27, 50
MflI RGATCY 1 cut(s) 196
MhlI GDGCHC 2 cut(s) 10, 296
MluCI AATT 3 cut(s) 27, 50, 240
MseI TTAA 1 cut(s) 78
MspA1I CMGCKG 1 cut(s) 266
MspI CCGG 1 cut(s) 120
MunI CAATTG 2 cut(s) 27, 50
NdeII GATC 2 cut(s) 130, 196
NmuCI GTSAC 1 cut(s) 115
PinAI ACCGGT 1 cut(s) 119
PkrI GCNGC 1 cut(s) 25
PspFI CCCAGC 1 cut(s) 155
PstI CTGCAG 1 cut(s) 304
PsuI RGATCY 1 cut(s) 196
SaqAI TTAA 1 cut(s) 78
SatI GCNGC 1 cut(s) 24
Sau3AI GATC 2 cut(s) 130, 196
SduI GDGCHC 2 cut(s) 10, 296
SetI ASST 6 cut(s) 171, 251, 262, 279, 309, 368
SfcI CTRYAG 1 cut(s) 300
SgeI CNNG 9 cut(s) 29, 49, 132, 146, 168, 182, 292, 360, 378
SmlI CTYRAG 1 cut(s) 278
SmoI CTYRAG 1 cut(s) 278
Sse9I AATT 3 cut(s) 27, 50, 240
SsiI CCGC 2 cut(s) 11, 264
TaiI ACGT 1 cut(s) 368
TasI AATT 3 cut(s) 27, 50, 240
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TseFI GTSAC 1 cut(s) 115
TseI GCWGC 1 cut(s) 23
Tsp45I GTSAC 1 cut(s) 115
TspGWI ACGGA 1 cut(s) 375
VneI GTGCAC 1 cut(s) 292
XapI RAATTY 1 cut(s) 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.